Pyrophosphate (PAMDB000633)

General function:

Involved in nucleic acid binding

Specific function:

In addition to polymerase activity, this DNA polymerase exhibits 3' to 5' and 5' to 3' exonuclease activity. It is able to utilize nicked circular duplex DNA as a template and can unwind the parental DNA strand from its template

Gene Name:

polA

Locus Tag:

PA5493

Molecular weight:

99.8 kDa

Reactions

General function:

Involved in anthranilate phosphoribosyltransferase activity

Specific function:

Chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate

Gene Name:

trpD

Locus Tag:

PA0650

Molecular weight:

37.4 kDa

Reactions

General function:

Involved in adenylate cyclase activity

Specific function:

ATP = 3',5'-cyclic AMP + diphosphate

Gene Name:

cyaA

Locus Tag:

PA5272

Molecular weight:

108.7 kDa

Reactions

General function:

Involved in nucleotide binding

Specific function:

ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp)

Gene Name:

trpS

Locus Tag:

PA4439

Molecular weight:

49 kDa

Reactions

General function:

Involved in nucleotide binding

Specific function:

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile)

Gene Name:

ileS

Locus Tag:

PA4560

Molecular weight:

105.5 kDa

Reactions

General function:

Involved in nucleotide binding

Specific function:

Edits incorrectly charged Ser-tRNA(Ala) and Gly- tRNA(Ala) but not incorrectly charged Ser-tRNA(Thr)

Gene Name:

alaS

Locus Tag:

PA0903

Molecular weight:

94.7 kDa

Reactions

General function:

Involved in nucleotide binding

Specific function:

Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation

Gene Name:

metG

Locus Tag:

PA3482

Molecular weight:

74.9 kDa

Reactions

General function:

Involved in nucleotide binding

Specific function:

ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-tRNA(Gly)

Gene Name:

glyQ

Locus Tag:

PA0009

Molecular weight:

36.1 kDa

Reactions

General function:

Involved in arginine-tRNA ligase activity

Specific function:

ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-tRNA(Gly)

Gene Name:

glyS

Locus Tag:

PA0008

Molecular weight:

74 kDa

Reactions

General function:

Involved in nucleotide binding

Specific function:

ATP + L-glutamine + tRNA(Gln) = AMP + diphosphate + L-glutaminyl-tRNA(Gln)

Gene Name:

glnS

Locus Tag:

PA1794

Molecular weight:

62.9 kDa

Reactions

General function:

Involved in DNA binding

Specific function:

DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. The epsilon subunit contain the editing function and is a proofreading 3'-5' exonuclease

Gene Name:

dnaQ

Locus Tag:

PA1816

Molecular weight:

26.8 kDa

Reactions

General function:

Involved in GMP synthase (glutamine-hydrolyzing) activity

Specific function:

Catalyzes the synthesis of GMP from XMP

Gene Name:

guaA

Locus Tag:

PA3769

Molecular weight:

58 kDa

Reactions

General function:

Involved in nucleotide binding

Specific function:

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

Gene Name:

gltX

Locus Tag:

PA3134

Molecular weight:

56.7 kDa

Reactions

General function:

Involved in lipopolysaccharide biosynthetic process

Specific function:

Activates KDO (a required 8-carbon sugar) for incorporation into bacterial lipopolysaccharide in Gram-negative bacteria

Gene Name:

kdsB

Locus Tag:

PA2979

Molecular weight:

27.6 kDa

Reactions

General function:

Involved in biotin-[acetyl-CoA-carboxylase] ligase activity

Specific function:

BirA acts both as a biotin-operon repressor and as the enzyme that synthesizes the corepressor, acetyl-CoA:carbon-dioxide ligase. This protein also activates biotin to form biotinyl-5'- adenylate and transfers the biotin moiety to biotin-accepting proteins

Gene Name:

birA

Locus Tag:

PA4280

Molecular weight:

34 kDa

Reactions

General function:

Involved in nucleotide binding

Specific function:

The gamma chain seems to interact with the delta subunit to transfer the beta subunit on the DNA

Gene Name:

dnaX

Locus Tag:

PA1532

Molecular weight:

73.3 kDa

Reactions

General function:

Involved in RNA binding

Specific function:

Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing inorganic pyrophosphate. Also shows highest phosphatase activity in the presence of Ni(2+) and hydrolyzes pyrophosphate, canonical 5'-nucleoside tri- and diphosphates, NADP, and 2'-AMP with the production of Pi. Displays a metal-independent phosphodiesterase activity toward 2',3'-cAMP, 2',3'-cGMP, and 2',3'-cCMP. Without metal or in the presence of Mg(2+), this protein hydrolyzes 2',3'-cyclic substrates with the formation of 2'-nucleotides, whereas in the presence of Ni(2+), it also produces some 3'-nucleotides. These phosphohydrolase activities are probably involved in the repair of the tRNA 3'-CCA terminus degraded by intracellular RNases

Gene Name:

cca

Locus Tag:

PA0584

Molecular weight:

45.6 kDa

Reactions

General function:

Involved in hydrolase activity

Specific function:

This enzyme is involved in nucleotide metabolism:it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA

Gene Name:

dut

Locus Tag:

PA5321

Molecular weight:

15.9 kDa

Reactions

General function:

Involved in phosphoribosyl-AMP cyclohydrolase activity

Specific function:

1-(5-phosphoribosyl)-ATP + H(2)O = 1-(5- phosphoribosyl)-AMP + diphosphate

Gene Name:

hisI

Locus Tag:

PA5066

Molecular weight:

15.4 kDa

Reactions

General function:

Involved in nucleotide binding

Specific function:

Catalyzes the attachment of valine to tRNA(Val). As ValRS can inadvertently accommodate and process structurally similar amino acids such as threonine, to avoid such errors, it has a "posttransfer" editing activity that hydrolyzes mischarged Thr-tRNA(Val) in a tRNA-dependent manner

Gene Name:

valS

Locus Tag:

PA3834

Molecular weight:

107.7 kDa

Reactions

General function:

Involved in RNA binding

Specific function:

ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe)

Gene Name:

pheT

Locus Tag:

PA2739

Molecular weight:

86.8 kDa

Reactions

General function:

Involved in nucleotide binding

Specific function:

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu)

Gene Name:

leuS

Locus Tag:

PA3987

Molecular weight:

97.6 kDa

Reactions

General function:

Involved in nucleotide binding

Specific function:

ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe)

Gene Name:

pheS

Locus Tag:

PA2740

Molecular weight:

38.1 kDa

Reactions

General function:

Involved in metabolic process

Specific function:

Catalyzes the removal of elemental sulfur and selenium atoms from cysteine and selenocysteine to produce alanine. Functions as a sulfur delivery protein for NAD, biotin and Fe-S cluster synthesis. Transfers sulfur on 'Cys-456' of thiI in a transpersulfidation reaction. Transfers sulfur on 'Cys-19' of tusA in a transpersulfidation reaction. Functions also as a selenium delivery protein in the pathway for the biosynthesis of selenophosphate

Gene Name:

iscS

Locus Tag:

PA3814

Molecular weight:

44.7 kDa

Reactions

General function:

Involved in argininosuccinate synthase activity

Specific function:

ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate

Gene Name:

argG

Locus Tag:

PA3525

Molecular weight:

45.3 kDa

Reactions

General function:

Involved in catalytic activity

Specific function:

Reversibly transfers an adenylyl group from ATP to 4'- phosphopantetheine, yielding dephospho-CoA (dPCoA) and pyrophosphate

Gene Name:

coaD

Locus Tag:

PA0363

Molecular weight:

17.8 kDa

Reactions

General function:

Involved in catalytic activity

Specific function:

Catalyzes the reversible adenylation of nicotinate mononucleotide (NaMN) to nicotinic acid adenine dinucleotide (NaAD)

Gene Name:

nadD

Locus Tag:

PA4006

Molecular weight:

23.8 kDa

Reactions

General function:

Involved in hydrolase activity

Specific function:

Master regulator of 5'-dependent mRNA decay. Accelerates the degradation of transcripts by removing pyrophosphate from the 5'-end of triphosphorylated RNA, leading to a more labile monophosphorylated state that can stimulate subsequent ribonuclease cleavage. Preferentially hydrolyzes diadenosine penta-phosphate with ATP as one of the reaction products. Also able to hydrolyze diadenosine hexa- and tetra-phosphate. Has no activity on diadenosine tri-phosphate, ADP-ribose, NADH and UDP- glucose. In the meningitis causing strain Pseudomonas aeruginosa K1, has been shown to play a role in HBMEC (human brain microvascular endothelial cells) invasion in vitro

Gene Name:

rppH

Locus Tag:

PA0336

Molecular weight:

18.7 kDa

General function:

Involved in magnesium ion binding

Specific function:

Diphosphate + H(2)O = 2 phosphate

Gene Name:

ppa

Locus Tag:

PA4031

Molecular weight:

19.4 kDa

Reactions

General function:

Involved in catalytic activity

Specific function:

Catalyzes the reversible transfer of the terminal phosphate of ATP to form a long-chain polyphosphate (polyP). Can form linear polymers of orthophosphate with chain lengths up to 1000 or more. Can also act in the reverse direction to form ATP in the presence of excess ADP. Can also use GTP instead of ATP; but the efficiency of GTP is 5% that of ATP

Gene Name:

ppk

Locus Tag:

PA5242

Molecular weight:

83.2 kDa

Reactions

General function:

Involved in orotate phosphoribosyltransferase activity

Specific function:

Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP)

Gene Name:

pyrE

Locus Tag:

PA5331

Molecular weight:

23.3 kDa

Reactions

General function:

Involved in GTP cyclohydrolase II activity

Specific function:

Catalyzes the conversion of GTP to 2,5-diamino-6- ribosylamino-4(3H)-pyrimidinone 5'-phosphate (DARP), formate and pyrophosphate

Gene Name:

ribA

Locus Tag:

PA4047

Molecular weight:

22.1 kDa

Reactions

General function:

Involved in DNA-directed RNA polymerase activity

Specific function:

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. This subunit plays an important role in subunit assembly since its dimerization is the first step in the sequential assembly of subunits to form the holoenzyme

Gene Name:

rpoA

Locus Tag:

PA4238

Molecular weight:

36.6 kDa

Reactions

General function:

Involved in DNA-directed RNA polymerase activity

Specific function:

Promotes RNA polymerase assembly. Latches the N- and C- terminal regions of the beta' subunit thereby facilitating its interaction with the beta and alpha subunits

Gene Name:

rpoZ

Locus Tag:

PA5337

Molecular weight:

9.8 kDa

Reactions

General function:

Involved in methionine adenosyltransferase activity

Specific function:

Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the enzyme. Is essential for growth

Gene Name:

metK

Locus Tag:

PA0546

Molecular weight:

42.7 kDa

Reactions

General function:

Involved in hydrolase activity

Specific function:

Nucleotidase with a broad substrate specificity as it can dephosphorylate various ribo- and deoxyribonucleoside 5'- monophosphates and ribonucleoside 3'-monophosphates with highest affinity to 3'-AMP. Also hydrolyzes polyphosphate (exopolyphosphatase activity) with the preference for short-chain- length substrates (P20-25). Might be involved in the regulation of dNTP and NTP pools, and in the turnover of 3'-mononucleotides produced by numerous intracellular RNases (T1, T2, and F) during the degradation of various RNAs. Also plays a significant physiological role in stress-response and is required for the survival of Pseudomonas aeruginosa in stationary growth phase

Gene Name:

surE

Locus Tag:

PA3625

Molecular weight:

26.4 kDa

Reactions

General function:

Involved in nucleoside metabolic process

Specific function:

Catalyzes the conversion of uracil and 5-phospho-alpha- D-ribose 1-diphosphate (PRPP) to UMP and diphosphate

Gene Name:

upp

Locus Tag:

PA4646

Molecular weight:

22.9 kDa

Reactions

General function:

Involved in nucleotide binding

Specific function:

Catalyzes the attachment of serine to tRNA(Ser). Is also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec)

Gene Name:

serS

Locus Tag:

PA2612

Molecular weight:

47.2 kDa

Reactions

General function:

Involved in nucleotide binding

Specific function:

ThrS is also a translational repressor protein, it controls the translation of its own gene by binding to its mRNA

Gene Name:

thrS

Locus Tag:

PA2744

Molecular weight:

73.1 kDa

Reactions

General function:

Involved in nucleotide binding

Specific function:

ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys)

Gene Name:

lysS

Locus Tag:

PA3700

Molecular weight:

57.3 kDa

Reactions

General function:

Involved in nucleotide binding

Specific function:

With YjeK might be involved in the post-translational modification of elongation factor P (EF-P)

Gene Name:

poxA

Locus Tag:

PA5513

Molecular weight:

32.4 kDa

General function:

Involved in DNA binding

Specific function:

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates

Gene Name:

rpoC

Locus Tag:

PA4269

Molecular weight:

154.4 kDa

Reactions

General function:

Involved in DNA binding

Specific function:

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates

Gene Name:

rpoB

Locus Tag:

PA4270

Molecular weight:

150.8 kDa

Reactions

General function:

Involved in DNA binding

Specific function:

DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped onto DNA, it slides freely (bidirectional and ATP- independent) along duplex DNA

Gene Name:

dnaN

Locus Tag:

PA0002

Molecular weight:

40.7 kDa

Reactions

General function:

Involved in RNA binding

Specific function:

Polymerase that creates the 3' poly(A) tail found in some mRNA's. Seems to be involved in plasmid copy number control

Gene Name:

pcnB

Locus Tag:

PA4727

Molecular weight:

53.3 kDa

Reactions

General function:

Involved in transferase activity, transferring phosphorus-containing groups

Specific function:

CTP + phosphatidate = diphosphate + CDP- diacylglycerol

Gene Name:

cdsA

Locus Tag:

PA3651

Molecular weight:

28.9 kDa

Reactions

General function:

Involved in phosphopantothenate--cysteine ligase activity

Specific function:

Catalyzes two steps in the biosynthesis of coenzyme A. In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4-phosphopantothenoylcysteine, in the latter compound is decarboxylated to form 4'-phosphopantotheine

Gene Name:

coaBC

Locus Tag:

PA5320

Molecular weight:

43.1 kDa

Reactions

General function:

Involved in dihydropteroate synthase activity

Specific function:

DHPS catalyzes the formation of the immediate precursor of folic acid. It is implicated in resistance to sulfonamide

Gene Name:

folP

Locus Tag:

PA4750

Molecular weight:

30.5 kDa

Reactions

General function:

Involved in magnesium ion binding

Specific function:

Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-GlcNAc. Responsible for the acetylation of Glc-N-1-P to give GlcNAc-1-P and for the uridyl transfer from UTP to GlcNAc-1-P which produces UDP-GlcNAc

Gene Name:

glmU

Locus Tag:

PA5552

Molecular weight:

48.9 kDa

Reactions

General function:

Involved in nucleotide binding

Specific function:

ATP-dependent phosphorylation of adenosylcobinamide and adds GMP to adenosylcobinamide phosphate

Gene Name:

cobU

Locus Tag:

PA1279

Molecular weight:

36.5 kDa

Reactions

General function:

Involved in UTP:glucose-1-phosphate uridylyltransferase activity

Specific function:

May play a role in stationary phase survival

Gene Name:

galU

Locus Tag:

PA2023

Molecular weight:

31.2 kDa

Reactions

General function:

Involved in nucleoside-triphosphate diphosphatase activity

Specific function:

Specific function unknown

Gene Name:

mazG

Locus Tag:

PA0935

Molecular weight:

31.2 kDa

Reactions

General function:

Involved in magnesium ion binding

Specific function:

Degradation of inorganic polyphosphates. Orthophosphate is released progressively from the ends of polyphosphate of circa 500 residues long, while chains of circa 15 residues compete poorly with polyphosphate as substrate

Gene Name:

ppx

Locus Tag:

PA5241

Molecular weight:

56.4 kDa

Reactions

General function:

Involved in amidophosphoribosyltransferase activity

Specific function:

5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H(2)O

Gene Name:

purF

Locus Tag:

PA3108

Molecular weight:

55.4 kDa

Reactions

General function:

Involved in catalytic activity

Specific function:

In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes both the synthesis and degradation of ppGpp. The second messengers ppGpp and c-di-GMP together control biofilm formation in response to translational stress; ppGpp represses biofilm formation while c- di-GMP induces it

Gene Name:

spoT

Locus Tag:

PA5338

Molecular weight:

78.9 kDa

Reactions

General function:

Involved in FMN adenylyltransferase activity

Specific function:

ATP + riboflavin = ADP + FMN

Gene Name:

ribF

Locus Tag:

PA4561

Molecular weight:

34.3 kDa

Reactions

General function:

Involved in nucleotide binding

Specific function:

Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr)

Gene Name:

tyrS

Locus Tag:

PA4138

Molecular weight:

45.1 kDa

Reactions

General function:

Involved in 3'-5' exonuclease activity

Specific function:

DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The alpha chain is the DNA polymerase

Gene Name:

dnaE

Locus Tag:

PA3640

Molecular weight:

130.9 kDa

Reactions

General function:

Involved in nucleotide binding

Specific function:

ATP + L-arginine + tRNA(Arg) = AMP + diphosphate + L-arginyl-tRNA(Arg)

Gene Name:

argS

Locus Tag:

PA5051

Molecular weight:

65.2 kDa

Reactions

General function:

Involved in ATP binding

Specific function:

Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

Gene Name:

miaA

Locus Tag:

PA4945

Molecular weight:

35.8 kDa

Reactions

General function:

Involved in nucleotide binding

Specific function:

Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids such as alanine and cysteine, to avoid such errors it has two additional distinct editing activities against alanine. One activity is designated as 'pretransfer' editing and involves the tRNA(Pro)-independent hydrolysis of activated Ala-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Ala-tRNA(Pro). Misacylated Cys-tRNA(Pro) is not edited by ProRS, but instead may be edited in trans by ybaK

Gene Name:

proS

Locus Tag:

PA0956

Molecular weight:

63.1 kDa

Reactions

General function:

Involved in nicotinate phosphoribosyltransferase activity

Specific function:

Nicotinate D-ribonucleotide + diphosphate = nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate

Gene Name:

pncB

Locus Tag:

PA4919

Molecular weight:

46.1 kDa

Reactions

General function:

Involved in NAD+ synthase (glutamine-hydrolyzing) activity

Specific function:

Catalyzes a key step in NAD biosynthesis, transforming deamido-NAD into NAD by a two-step reaction

Gene Name:

nadE

Locus Tag:

PA4920

Molecular weight:

29.7 kDa

Reactions

General function:

Involved in catalytic activity

Specific function:

ATP + sulfate = diphosphate + adenylyl sulfate

Gene Name:

cysD

Locus Tag:

PA4443

Molecular weight:

35.5 kDa

Reactions

General function:

Involved in nucleotide binding

Specific function:

Thought to be involved in DNA repair and/or mutagenesis

Gene Name:

polB

Locus Tag:

PA1886

Molecular weight:

89.7 kDa

Reactions

General function:

Involved in cysteine-tRNA ligase activity

Specific function:

ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys)

Gene Name:

cysS

Locus Tag:

PA1795

Molecular weight:

51.2 kDa

Reactions

General function:

Involved in nucleotide binding

Specific function:

ATP + L-aspartate + tRNA(Asp) = AMP + diphosphate + L-aspartyl-tRNA(Asp)

Gene Name:

aspS

Locus Tag:

PA0963

Molecular weight:

66.2 kDa

Reactions

General function:

Involved in isoprenoid biosynthetic process

Specific function:

Geranyl diphosphate + isopentenyl diphosphate = diphosphate + trans,trans-farnesyl diphosphate

Gene Name:

ispA

Locus Tag:

PA4043

Molecular weight:

31.5 kDa

Reactions

General function:

Involved in GTPase activity

Specific function:

May be the GTPase, regulating ATP sulfurylase activity

Gene Name:

cysN

Locus Tag:

PA4442

Molecular weight:

69.3 kDa

Reactions

General function:

Involved in nucleotidyltransferase activity

Specific function:

Involved in the biosynthesis of the capsular polysaccharide colanic acid

Gene Name:

manC

Locus Tag:

PA3551

Molecular weight:

53.1 kDa

Reactions

General function:

Involved in sulfurtransferase activity

Specific function:

Catalyzes the 2-thiolation of uridine at the wobble position (U34) of tRNA(Lys), tRNA(Glu) and tRNA(Gln), leading to the formation of s(2)U34, the first step of tRNA-mnm(5)s(2)U34 synthesis. Sulfur is provided by iscS, via a sulfur-relay system. Binds ATP and its substrate tRNAs

Gene Name:

mnmA

Locus Tag:

PA2626

Molecular weight:

41.9 kDa

General function:

Involved in amino acid binding

Specific function:

Modifies, by uridylylation or deuridylylation the PII (glnB) regulatory protein

Gene Name:

glnD

Locus Tag:

PA3658

Molecular weight:

103.4 kDa

Reactions

General function:

Involved in acetate-CoA ligase activity

Specific function:

Enables the cell to use acetate during aerobic growth to generate energy via the TCA cycle, and biosynthetic compounds via the glyoxylate shunt. Acetylates CheY, the response regulator involved in flagellar movement and chemotaxis

Gene Name:

acs

Locus Tag:

PA0887

Molecular weight:

71.8 kDa

Reactions

General function:

Involved in DNA binding

Specific function:

DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The delta subunit seems to interact with the gamma subunit to transfer the beta subunit on the DNA

Gene Name:

holA

Locus Tag:

PA3989

Molecular weight:

37.4 kDa

Reactions

General function:

Involved in DNA binding

Specific function:

DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity

Gene Name:

holB

Locus Tag:

PA2961

Molecular weight:

35.7 kDa

Reactions

General function:

Involved in DNA binding

Specific function:

DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity

Gene Name:

holC

Locus Tag:

PA3832

Molecular weight:

16.1 kDa

Reactions

General function:

Involved in catalytic activity

Specific function:

Involved in the catabolism of quinolinic acid (QA)

Gene Name:

nadC

Locus Tag:

PA4524

Molecular weight:

30.6 kDa

Reactions

General function:

Involved in catalytic activity

Specific function:

Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP)

Gene Name:

thiE

Locus Tag:

PA3976

Molecular weight:

22.1 kDa

Reactions

General function:

Involved in [glutamate-ammonia-ligase] adenylyltransferase activity

Specific function:

Adenylation and deadenylation of glutamate--ammonia ligase

Gene Name:

glnE

Locus Tag:

PA5014

Molecular weight:

110.1 kDa

Reactions

General function:

Involved in catalytic activity

Specific function:

Catalyzes the condensation of pantoate with beta-alanine in an ATP-dependent reaction via a pantoyl-adenylate intermediate

Gene Name:

panC

Locus Tag:

PA4730

Molecular weight:

30.8 kDa

Reactions

General function:

Involved in ATP binding

Specific function:

Catalyzes two reactions:the first one is the production of beta-formyl glycinamide ribonucleotide (GAR) from formate, ATP and beta GAR; the second, a side reaction, is the production of acetyl phosphate and ADP from acetate and ATP

Gene Name:

purT

Locus Tag:

PA3751

Molecular weight:

42.3 kDa

Reactions

General function:

Involved in glucose-1-phosphate thymidylyltransferase activity

Specific function:

Catalyzes the formation of dTDP-glucose, from dTTP and glucose 1-phosphate, as well as its pyrophosphorolysis

Gene Name:

rmlA1

Locus Tag:

PA5163

Molecular weight:

32.5 kDa

Reactions

General function:

Involved in RNA processing

Specific function:

Catalyzes the conversion of 3'-phosphate to a 2',3'- cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps:(A) adenylation of the enzyme by ATP; (B) the enzyme acts on RNA-N3'P to produce RNA-N3'PP5'A; (C) a non catalytic nucleophilic attack by the adjacent 2'hydroxyl on the phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing

Gene Name:

rtcA

Locus Tag:

PA4585

Molecular weight:

36.6 kDa

Reactions

General function:

Involved in hydrolase activity

Specific function:

Hydrolyzes O6 atom-containing purine bases deoxyinosine triphosphate (dITP) and xanthosine triphosphate (XTP) as well as 2'-deoxy-N-6-hydroxylaminopurine triposphate (dHAPTP) to nucleotide monophosphate and pyrophosphate. Probably excludes non- standard purines from DNA precursor pool, preventing thus incorporation into DNA and avoiding chromosomal lesions

Gene Name:

rdgB

Locus Tag:

PA0387

Molecular weight:

21.2 kDa

Reactions

General function:

Involved in transferase activity, transferring alkyl or aryl (other than methyl) groups

Specific function:

Generates undecaprenyl pyrophosphate (UPP) from isopentenyl pyrophosphate (IPP). UPP is the precursor of glycosyl carrier lipid in the biosynthesis of bacterial cell wall polysaccharide components such as peptidoglycan and lipopolysaccharide

Gene Name:

uppS

Locus Tag:

PA3652

Molecular weight:

28 kDa

Reactions

General function:

Involved in ATP phosphoribosyltransferase activity

Specific function:

Catalyzes the condensation of ATP and 5-phosphoribose 1- diphosphate to form N'-(5'-phosphoribosyl)-ATP (PR-ATP). Has a crucial role in the pathway because the rate of histidine biosynthesis seems to be controlled primarily by regulation of hisG enzymatic activity

Gene Name:

hisG

Locus Tag:

PA4449

Molecular weight:

22.8 kDa

Reactions

General function:

Involved in nucleotide binding

Specific function:

ATP + L-histidine + tRNA(His) = AMP + diphosphate + L-histidyl-tRNA(His)

Gene Name:

hisS

Locus Tag:

PA3802

Molecular weight:

47.5 kDa

Reactions

General function:

Involved in catalytic activity

Specific function:

Catalyzes the esterification, concomitant with transport, of exogenous long-chain fatty acids into metabolically active CoA thioesters for subsequent degradation or incorporation into phospholipids

Gene Name:

fadD

Locus Tag:

PA3299

Molecular weight:

61.7 kDa

Reactions

General function:

Involved in adenine phosphoribosyltransferase activity

Specific function:

Catalyzes a salvage reaction resulting in the formation of AMP, that is energically less costly than de novo synthesis

Gene Name:

apt

Locus Tag:

PA1543

Molecular weight:

19.8 kDa

Reactions

General function:

Involved in damaged DNA binding

Specific function:

Poorly processive, error-prone DNA polymerase involved in untargeted mutagenesis. Copies undamaged DNA at stalled replication forks, which arise in vivo from mismatched or misaligned primer ends. These misaligned primers can be extended by polIV. Exhibits no 3'-5' exonuclease (proofreading) activity. Overexpression of polIV results in increased frameshift mutagenesis. It is required for stationary-phase adaptive mutation, which provides the bacterium with flexibility in dealing with environmental stress, enhancing long-term survival and evolutionary fitness. May be involved in translesional synthesis, in conjunction with the beta clamp from polIII

Gene Name:

dinB

Locus Tag:

PA0923

Molecular weight:

38.9 kDa

Reactions

General function:

Involved in phosphotransferase activity, alcohol group as acceptor

Specific function:

Catalyzes the ADP transfer to D-glycero-D-manno-heptose 1-phosphate, yielding ADP-D,D-heptose

Gene Name:

hldE

Locus Tag:

PA4996

Molecular weight:

50.3 kDa

Reactions

General function:

Involved in catalytic activity

Specific function:

Links a guanosine 5'-phosphate to molydopterin (MPT) forming molybdopterin guanine dinucleotide (MGD)

Gene Name:

mobA

Locus Tag:

PA3030

Molecular weight:

21.9 kDa

Reactions

General function:

Involved in prenyltransferase activity

Specific function:

Synthesis of 3-octaprenyl-4-hydroxybenzoate

Gene Name:

ubiA

Locus Tag:

PA5358

Molecular weight:

33 kDa

Reactions

General function:

Involved in RNA binding

Specific function:

Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. This is a step in the synthesis of thiazole, in the thiamine biosynthesis pathway. The sulfur is donated as persulfide by iscS

Gene Name:

thiI

Locus Tag:

PA5118

Molecular weight:

54.8 kDa

Reactions

General function:

Involved in isoprenoid biosynthetic process

Specific function:

Supplies octaprenyl diphosphate, the precursor for the side chain of the isoprenoid quinones ubiquinone and menaquinone

Gene Name:

ispB

Locus Tag:

PA4569

Molecular weight:

34.9 kDa

Reactions

General function:

Involved in protoheme IX farnesyltransferase activity

Specific function:

Converts heme B (protoheme IX) to heme O by substitution of the vinyl group on carbon 2 of heme B porphyrin ring with a hydroxyethyl farnesyl side group

Gene Name:

cyoE

Locus Tag:

PA1321

Molecular weight:

31.8 kDa

General function:

Involved in Mo-molybdopterin cofactor biosynthetic process

Specific function:

Catalyzes the adenylation by ATP of the carboxyl group of the C-terminal glycine of sulfur carrier protein moaD

Gene Name:

moeB

Locus Tag:

PA4663

Molecular weight:

26.7 kDa

Reactions

General function:

Involved in Mo-molybdopterin cofactor biosynthetic process

Specific function:

Together with moaA, is involved in the conversion of a guanosine derivative (5'-GTP) into molybdopterin precursor Z

Gene Name:

moaC

Locus Tag:

PA3918

Molecular weight:

17.3 kDa

General function:

Involved in fatty acid biosynthetic process

Specific function:

Carrier of the growing fatty acid chain in fatty acid biosynthesis

Gene Name:

acpP

Locus Tag:

PA2966

Molecular weight:

8.7 kDa

General function:

Replication, recombination and repair

Specific function:

Involved in the GO system responsible for removing an oxidatively damaged form of guanine (7,8-dihydro-8-oxoguanine) from DNA and the nucleotide pool. 8-oxo-dGTP is inserted opposite dA and dC residues of template DNA with almost equal efficiency thus leading to A.T to G.C transversions. MutT specifically degrades 8-oxo-dGTP to the monophosphate

Gene Name:

mutT

Locus Tag:

PA4400

Molecular weight:

34 kDa

Reactions

General function:

threonylcarbamoyladenosine biosynthetic process

Specific function:

Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. Catalyzes the conversion of L-threonine, bicarbonate/CO(2) and ATP to give threonylcarbamoyl-AMP (TC-AMP) as the acyladenylate intermediate, with the release of pyrophosphate. Is also able to catalyze the reverse reaction in vitro, i.e. the formation of ATP from TC-AMP and PPi. Shows higher affinity for the full-length tRNA(Thr) lacking only the t(6)A37 modification than for its fully modified counterpart. Could also be required for the maturation of 16S rRNA. Binds to double-stranded RNA but does not interact tightly with either of the ribosomal subunits, or the 70S particles.

Gene Name:

tsaC

Locus Tag:

PA0022

Molecular weight:

20.4 kDa

Reactions

General function:

organic phosphonate catabolic process

Specific function:

Catalyzes the hydrolysis of alpha-D-ribose 1-methylphosphonate triphosphate (RPnTP) to form alpha-D-ribose 1-methylphosphonate phosphate (PRPn) and diphosphate.

Gene Name:

phnM

Locus Tag:

PA3374

Molecular weight:

41.6 kDa

Reactions