Tyrosyl-tRNA synthetase (PA4138)

Identification
Name: Tyrosyl-tRNA synthetase
Synonyms:
  • Tyrosine--tRNA ligase
  • TyrRS
Gene Name: tyrS
Enzyme Class:
Biological Properties
General Function: Involved in nucleotide binding
Specific Function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr)
Cellular Location: Cytoplasm
KEGG Pathways:
KEGG Reactions:
Thumb+tRNA(Tyr)+Thumb+Thumb ↔ Thumb+Thumb+L-Tyrosyl-tRNA(Tyr)+Thumb
Adenosine triphosphate + tRNA(Tyr) + L-Tyrosine + tRNA(Tyr) ↔ Adenosine monophosphate + Pyrophosphate + L-Tyrosyl-tRNA(Tyr) + L-Tyrosyl-tRNA(Tyr)
ReactionCard
Thumb+Thumb+tRNA(Tyr) ↔ Thumb+Thumb+L-Tyrosyl-tRNA(Tyr)
Adenosine triphosphate + L-Tyrosine + tRNA(Tyr) ↔ Adenosine monophosphate + Pyrophosphate + L-Tyrosyl-tRNA(Tyr)
ReactionCard
SMPDB Reactions:
L-Tyrosine+Thumb+Thumb+tRNA(Tyr)+Thumb → Thumb+Pyrophosphate+L-tyrosyl-tRNA(Tyr)
L-Tyrosine + Adenosine triphosphate + Hydrogen ion + tRNA(Tyr) + L-Tyrosine → Adenosine monophosphate + Pyrophosphate + L-tyrosyl-tRNA(Tyr)
ReactionCard
PseudoCyc/BioCyc Reactions:
Thumb+tRNA(Tyr)+Thumb+Thumb ↔ Thumb+Thumb+L-Tyrosyl-tRNA(Tyr)+Thumb
Adenosine triphosphate + tRNA(Tyr) + L-Tyrosine + tRNA(Tyr) ↔ Adenosine monophosphate + Pyrophosphate + L-Tyrosyl-tRNA(Tyr) + L-Tyrosyl-tRNA(Tyr)
ReactionCard
Thumb+Thumb+tRNA(Tyr) ↔ Thumb+Thumb+L-Tyrosyl-tRNA(Tyr)
Adenosine triphosphate + L-Tyrosine + tRNA(Tyr) ↔ Adenosine monophosphate + Pyrophosphate + L-Tyrosyl-tRNA(Tyr)
ReactionCard
Thumb+Thumb+tRNA(Tyr) → Thumb+Thumb+L-tyrosyl-tRNA(Tyr)
Adenosine triphosphate + L-Tyrosine + tRNA(Tyr) → Adenosine monophosphate + Pyrophosphate + L-tyrosyl-tRNA(Tyr)
ReactionCard
L-Tyrosine+Thumb+Thumb+tRNA(Tyr)+Thumb → Thumb+Pyrophosphate+L-tyrosyl-tRNA(Tyr)
L-Tyrosine + Adenosine triphosphate + Hydrogen ion + tRNA(Tyr) + L-Tyrosine → Adenosine monophosphate + Pyrophosphate + L-tyrosyl-tRNA(Tyr)
ReactionCard
Thumb
a tRNAtyr + L-tyrosine + ATP + H+ → an L-tyrosyl-[tRNAtyr] + AMP + diphosphate
ReactionCard
Complex Reactions:
Thumb+Thumb+tRNA(Tyr) → Thumb+Thumb+L-tyrosyl-tRNA(Tyr)
Adenosine triphosphate + L-Tyrosine + tRNA(Tyr) → Adenosine monophosphate + Pyrophosphate + L-tyrosyl-tRNA(Tyr)
ReactionCard
Transports: Not Available
Metabolites:
PAMDB IDNameView
PAMDB000014Adenosine monophosphateMetaboCard
PAMDB000148Adenosine triphosphateMetaboCard
PAMDB001633Hydrogen ionMetaboCard
PAMDB000060L-TyrosineMetaboCard
PAMDB004405L-Tyrosyl-tRNA(Tyr)MetaboCard
PAMDB000633PyrophosphateMetaboCard
PAMDB005478tRNA(Tyr)MetaboCard
GO Classification:
Component
cell part
cytoplasm
intracellular part
Function
adenyl nucleotide binding
adenyl ribonucleotide binding
aminoacyl-tRNA ligase activity
ATP binding
binding
catalytic activity
ligase activity
ligase activity, forming aminoacyl-tRNA and related compounds
ligase activity, forming carbon-oxygen bonds
nucleic acid binding
nucleoside binding
nucleotide binding
purine nucleoside binding
RNA binding
tyrosine-tRNA ligase activity
Process
biosynthetic process
cellular macromolecule biosynthetic process
cellular macromolecule metabolic process
macromolecule biosynthetic process
macromolecule metabolic process
metabolic process
ncRNA metabolic process
RNA metabolic process
translation
tRNA aminoacylation
tRNA aminoacylation for protein translation
tRNA metabolic process
tyrosyl-tRNA aminoacylation
Gene Properties
Locus tag: PA4138
Strand: -
Entrez Gene ID: 880105
Accession: NP_252827.1
GI: 15599333
Sequence start: 4627947
Sequence End: 4629185
Sequence Length: 1238
Gene Sequence: 
>PA4138
GTGTCCGTCCCGACCCATCAGCAAGACCTGATCGCCCTGCTCGAAGAGCGTGGCTTCGTCCACCAGTGCACCGACCGCGACGGCCTCGCCGCGCACCTGGCCGCCGGTCCGGCAACCGCCTACCTGGGCTTCGACGCCACCGCCGACAGCCTGCACGTCGGCCACCTACAGGGCCTGATGCTGATGCGCTGGCTACAGAAGGCCGGGCACCGCCCGTTGCTGCTGATCGGTGGCGCGACCACCCGGATCGGCGATCCGAGCTTCCGCGATTCGAGCCGGCCGATCCTCACCGAGGCGCAGATCCAGGCCAACATCGACGGCATCGCCCGGGTTTTCTCGCGCTACGTCGAACTGCACGACGACAGCCTGGTGAACAACGCCGAATGGCTGGACGGCGTCGGCTACCTGGAGTTCCTCGACCGGGTCGGCCGGCACTTCTCGATCAACCGCCTGCTGACCTTCGACGCCATCAGGCAGCGCCTGGACCGCGAGCATTCGCTGTCGTTCCTCGAGTTCGGCTACACCCTGCTGCAGGCCTACGATTTCGTCGAACTGTCGCGCCGGCGCGGCTGCACCCTGCAACTCGGCGGCGCCGACCAGTGGGCGAACATCATCAACGGCGTGGAGCTGTCGCGACGCCAGGGCGGCGCCCAGCTGTTCGGCCTGACCATGCCGCTGCTGGCCACCAGTGACGGGCGCAAGATGGGCAAGTCGGCGCAGGGTGCGGTATGGCTCAACGCCGAGCGCCTGGCGCCGTTCGACTTCTGGCAGTTCTGGCGCAACTGCGATGATCGCGACGTCGGTCGCTTCCTCGCCCTGTTCAGCGAACTGCCGATGGATGAGGTGCGCCGCCTGGGCGCCTTGCAGGGCGCCGAGCTGAACGAAGCGAAGGTGGTCCTGGCCAATGCCGCCACCGCCCTGGCGCACGGCGAGCACGCCGCGCGATCCGCCGCCGATGCCGCGCGCGGGGTGTTCGCCGACGGTACGCGGGACAGCGGCCTGCCGGTGATGAAGCTATCCCGCGCGCGCCTGGCGCAGGGACTGTCGCTGACCGACCTGCTGCTGGAACACGCGATCCAGCCCTCCCGCAGCGCGGTCCGCCGCCTCGCCGCCGGCGGCGGCCTGCGCCTGGACGGCACGCCGGTCAGCGATCCGGACACGCCGCTGGCGGGCGAGGTCGACGGGCTGCGCTTGAGCCTGGGCAAGAAGCAGCATTTGCACCTGCGACTCGAGGATTGA
Protein Properties
Protein Residues: 412
Protein Molecular Weight: 45.1 kDa
Protein Theoretical pI: 6.76
Hydropathicity (GRAVY score): -0.156
Charge at pH 7 (predicted): -1.71
Protein Sequence: 
>PA4138
MSVPTHQQDLIALLEERGFVHQCTDRDGLAAHLAAGPATAYLGFDATADSLHVGHLQGLMLMRWLQKAGHRPLLLIGGATTRIGDPSFRDSSRPILTEAQIQANIDGIARVFSRYVELHDDSLVNNAEWLDGVGYLEFLDRVGRHFSINRLLTFDAIRQRLDREHSLSFLEFGYTLLQAYDFVELSRRRGCTLQLGGADQWANIINGVELSRRQGGAQLFGLTMPLLATSDGRKMGKSAQGAVWLNAERLAPFDFWQFWRNCDDRDVGRFLALFSELPMDEVRRLGALQGAELNEAKVVLANAATALAHGEHAARSAADAARGVFADGTRDSGLPVMKLSRARLAQGLSLTDLLLEHAIQPSRSAVRRLAAGGGLRLDGTPVSDPDTPLAGEVDGLRLSLGKKQHLHLRLED
References
External Links:
Resource Link
Genome ID: PA4138
Entrez Gene ID: 880105
NCBI Protein ID: 15599333
General Reference: PaperBLAST - Find papers about PA4138 and its homologs