Adenosine monophosphate (PAMDB000014)

General function:

Involved in catalytic activity

Specific function:

A phosphate monoester + H(2)O = an alcohol + phosphate

Gene Name:

phoA

Locus Tag:

PA3296

Molecular weight:

50.4 kDa

Reactions

General function:

Involved in nucleotide binding

Specific function:

ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp)

Gene Name:

trpS

Locus Tag:

PA4439

Molecular weight:

49 kDa

Reactions

General function:

Involved in nucleotide binding

Specific function:

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile)

Gene Name:

ileS

Locus Tag:

PA4560

Molecular weight:

105.5 kDa

Reactions

General function:

Involved in nucleotide binding

Specific function:

Edits incorrectly charged Ser-tRNA(Ala) and Gly- tRNA(Ala) but not incorrectly charged Ser-tRNA(Thr)

Gene Name:

alaS

Locus Tag:

PA0903

Molecular weight:

94.7 kDa

Reactions

General function:

Involved in nucleotide binding

Specific function:

Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation

Gene Name:

metG

Locus Tag:

PA3482

Molecular weight:

74.9 kDa

Reactions

General function:

Involved in nucleotide binding

Specific function:

ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-tRNA(Gly)

Gene Name:

glyQ

Locus Tag:

PA0009

Molecular weight:

36.1 kDa

Reactions

General function:

Involved in arginine-tRNA ligase activity

Specific function:

ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-tRNA(Gly)

Gene Name:

glyS

Locus Tag:

PA0008

Molecular weight:

74 kDa

Reactions

General function:

Involved in nucleotide binding

Specific function:

ATP + L-glutamine + tRNA(Gln) = AMP + diphosphate + L-glutaminyl-tRNA(Gln)

Gene Name:

glnS

Locus Tag:

PA1794

Molecular weight:

62.9 kDa

Reactions

General function:

Involved in GMP synthase (glutamine-hydrolyzing) activity

Specific function:

Catalyzes the synthesis of GMP from XMP

Gene Name:

guaA

Locus Tag:

PA3769

Molecular weight:

58 kDa

Reactions

General function:

Involved in nucleotide binding

Specific function:

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

Gene Name:

gltX

Locus Tag:

PA3134

Molecular weight:

56.7 kDa

Reactions

General function:

Involved in biotin-[acetyl-CoA-carboxylase] ligase activity

Specific function:

BirA acts both as a biotin-operon repressor and as the enzyme that synthesizes the corepressor, acetyl-CoA:carbon-dioxide ligase. This protein also activates biotin to form biotinyl-5'- adenylate and transfers the biotin moiety to biotin-accepting proteins

Gene Name:

birA

Locus Tag:

PA4280

Molecular weight:

34 kDa

Reactions

General function:

Involved in nucleotide binding

Specific function:

Catalyzes the attachment of valine to tRNA(Val). As ValRS can inadvertently accommodate and process structurally similar amino acids such as threonine, to avoid such errors, it has a "posttransfer" editing activity that hydrolyzes mischarged Thr-tRNA(Val) in a tRNA-dependent manner

Gene Name:

valS

Locus Tag:

PA3834

Molecular weight:

107.7 kDa

Reactions

General function:

Involved in RNA binding

Specific function:

ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe)

Gene Name:

pheT

Locus Tag:

PA2739

Molecular weight:

86.8 kDa

Reactions

General function:

Involved in nucleotide binding

Specific function:

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu)

Gene Name:

leuS

Locus Tag:

PA3987

Molecular weight:

97.6 kDa

Reactions

General function:

Involved in nucleotide binding

Specific function:

ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe)

Gene Name:

pheS

Locus Tag:

PA2740

Molecular weight:

38.1 kDa

Reactions

General function:

Involved in metabolic process

Specific function:

Catalyzes the removal of elemental sulfur and selenium atoms from cysteine and selenocysteine to produce alanine. Functions as a sulfur delivery protein for NAD, biotin and Fe-S cluster synthesis. Transfers sulfur on 'Cys-456' of thiI in a transpersulfidation reaction. Transfers sulfur on 'Cys-19' of tusA in a transpersulfidation reaction. Functions also as a selenium delivery protein in the pathway for the biosynthesis of selenophosphate

Gene Name:

iscS

Locus Tag:

PA3814

Molecular weight:

44.7 kDa

Reactions

General function:

Involved in argininosuccinate synthase activity

Specific function:

ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate

Gene Name:

argG

Locus Tag:

PA3525

Molecular weight:

45.3 kDa

Reactions

General function:

Involved in magnesium ion binding

Specific function:

ATP + D-ribose 5-phosphate = AMP + 5-phospho- alpha-D-ribose 1-diphosphate

Gene Name:

prs

Locus Tag:

PA4670

Molecular weight:

34 kDa

Reactions

General function:

Involved in hydrolase activity

Specific function:

Nucleotidase with a broad substrate specificity as it can dephosphorylate various ribo- and deoxyribonucleoside 5'- monophosphates and ribonucleoside 3'-monophosphates with highest affinity to 3'-AMP. Also hydrolyzes polyphosphate (exopolyphosphatase activity) with the preference for short-chain- length substrates (P20-25). Might be involved in the regulation of dNTP and NTP pools, and in the turnover of 3'-mononucleotides produced by numerous intracellular RNases (T1, T2, and F) during the degradation of various RNAs. Also plays a significant physiological role in stress-response and is required for the survival of Pseudomonas aeruginosa in stationary growth phase

Gene Name:

surE

Locus Tag:

PA3625

Molecular weight:

26.4 kDa

Reactions

General function:

Involved in nucleotide binding

Specific function:

Catalyzes the attachment of serine to tRNA(Ser). Is also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec)

Gene Name:

serS

Locus Tag:

PA2612

Molecular weight:

47.2 kDa

Reactions

General function:

Involved in nucleotide binding

Specific function:

ThrS is also a translational repressor protein, it controls the translation of its own gene by binding to its mRNA

Gene Name:

thrS

Locus Tag:

PA2744

Molecular weight:

73.1 kDa

Reactions

General function:

Involved in nucleotide binding

Specific function:

ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys)

Gene Name:

lysS

Locus Tag:

PA3700

Molecular weight:

57.3 kDa

Reactions

General function:

Involved in nucleotide binding

Specific function:

With YjeK might be involved in the post-translational modification of elongation factor P (EF-P)

Gene Name:

poxA

Locus Tag:

PA5513

Molecular weight:

32.4 kDa

General function:

Involved in N6-(1,2-dicarboxyethyl)AMP AMP-lyase (fumarate-forming) activity

Specific function:

N(6)-(1,2-dicarboxyethyl)AMP = fumarate + AMP

Gene Name:

purB

Locus Tag:

PA2629

Molecular weight:

50.5 kDa

Reactions

General function:

Involved in phosphopantothenate--cysteine ligase activity

Specific function:

Catalyzes two steps in the biosynthesis of coenzyme A. In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4-phosphopantothenoylcysteine, in the latter compound is decarboxylated to form 4'-phosphopantotheine

Gene Name:

coaBC

Locus Tag:

PA5320

Molecular weight:

43.1 kDa

Reactions

General function:

Involved in catalytic activity

Specific function:

Involved in regulation of AMP concentrations

Gene Name:

amn

Locus Tag:

PA3970

Molecular weight:

56.2 kDa

Reactions

General function:

Involved in acid phosphatase activity

Specific function:

Dephosphorylates several organic phosphomonoesters and catalyzes the transfer of low-energy phosphate groups from phosphomonoesters to hydroxyl groups of various organic compounds. Preferentially acts on aryl phosphoesters. Might function as a broad-spectrum dephosphorylating enzyme able to scavenge both 3'- and 5'-nucleotides and also additional organic phosphomonoesters

Gene Name:

aphA

Locus Tag:

PA1409

Molecular weight:

38 kDa

Reactions

General function:

Involved in nucleoside-triphosphate diphosphatase activity

Specific function:

Specific function unknown

Gene Name:

mazG

Locus Tag:

PA0935

Molecular weight:

31.2 kDa

Reactions

General function:

Involved in amino acid binding

Specific function:

In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the formation of pppGpp which is then hydrolyzed to form ppGpp

Gene Name:

relA

Locus Tag:

PA0934

Molecular weight:

83.8 kDa

Reactions

General function:

Involved in catalytic activity

Specific function:

In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes both the synthesis and degradation of ppGpp. The second messengers ppGpp and c-di-GMP together control biofilm formation in response to translational stress; ppGpp represses biofilm formation while c- di-GMP induces it

Gene Name:

spoT

Locus Tag:

PA5338

Molecular weight:

78.9 kDa

Reactions

General function:

Involved in nucleotide binding

Specific function:

Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr)

Gene Name:

tyrS

Locus Tag:

PA4138

Molecular weight:

45.1 kDa

Reactions

General function:

Involved in nucleotide binding

Specific function:

ATP + L-arginine + tRNA(Arg) = AMP + diphosphate + L-arginyl-tRNA(Arg)

Gene Name:

argS

Locus Tag:

PA5051

Molecular weight:

65.2 kDa

Reactions

General function:

Involved in DNA ligase (NAD+) activity

Specific function:

DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA

Gene Name:

ligA

Locus Tag:

PA1529

Molecular weight:

86.8 kDa

Reactions

General function:

Involved in catalytic activity

Specific function:

Synthesizes selenophosphate from selenide and ATP

Gene Name:

selD

Locus Tag:

PA1642

Molecular weight:

36.4 kDa

Reactions

General function:

Involved in nucleotide binding

Specific function:

Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids such as alanine and cysteine, to avoid such errors it has two additional distinct editing activities against alanine. One activity is designated as 'pretransfer' editing and involves the tRNA(Pro)-independent hydrolysis of activated Ala-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Ala-tRNA(Pro). Misacylated Cys-tRNA(Pro) is not edited by ProRS, but instead may be edited in trans by ybaK

Gene Name:

proS

Locus Tag:

PA0956

Molecular weight:

63.1 kDa

Reactions

General function:

Involved in NAD+ synthase (glutamine-hydrolyzing) activity

Specific function:

Catalyzes a key step in NAD biosynthesis, transforming deamido-NAD into NAD by a two-step reaction

Gene Name:

nadE

Locus Tag:

PA4920

Molecular weight:

29.7 kDa

Reactions

General function:

Involved in cysteine-tRNA ligase activity

Specific function:

ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys)

Gene Name:

cysS

Locus Tag:

PA1795

Molecular weight:

51.2 kDa

Reactions

General function:

Involved in nucleotide binding

Specific function:

ATP + L-aspartate + tRNA(Asp) = AMP + diphosphate + L-aspartyl-tRNA(Asp)

Gene Name:

aspS

Locus Tag:

PA0963

Molecular weight:

66.2 kDa

Reactions

General function:

Involved in magnesium ion binding

Specific function:

Converts 3'(2')-phosphoadenosine 5'-phosphate (PAP) to AMP. May also convert adenosine 3'-phosphate 5'-phosphosulfate (PAPS) to adenosine 5'-phosphosulfate (APS). Has 10000-fold lower activity towards inositol 1,4-bisphosphate (Ins(1,4)P2)

Gene Name:

cysQ

Locus Tag:

PA5175

Molecular weight:

29.8 kDa

Reactions

General function:

Involved in catalytic activity

Specific function:

Catalyzes the phosphorylation of pyruvate to phosphoenolpyruvate

Gene Name:

ppsA

Locus Tag:

PA1770

Molecular weight:

85.8 kDa

Reactions

General function:

Involved in sulfurtransferase activity

Specific function:

Catalyzes the 2-thiolation of uridine at the wobble position (U34) of tRNA(Lys), tRNA(Glu) and tRNA(Gln), leading to the formation of s(2)U34, the first step of tRNA-mnm(5)s(2)U34 synthesis. Sulfur is provided by iscS, via a sulfur-relay system. Binds ATP and its substrate tRNAs

Gene Name:

mnmA

Locus Tag:

PA2626

Molecular weight:

41.9 kDa

General function:

Involved in 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase activity

Specific function:

ATP + 2-amino-4-hydroxy-6-hydroxymethyl-7,8- dihydropteridine = AMP + (2-amino-4-hydroxy-7,8-dihydropteridin-6- yl)methyl diphosphate

Gene Name:

folK

Locus Tag:

PA4728

Molecular weight:

18 kDa

Reactions

General function:

Involved in acetate-CoA ligase activity

Specific function:

Enables the cell to use acetate during aerobic growth to generate energy via the TCA cycle, and biosynthetic compounds via the glyoxylate shunt. Acetylates CheY, the response regulator involved in flagellar movement and chemotaxis

Gene Name:

acs

Locus Tag:

PA0887

Molecular weight:

71.8 kDa

Reactions

General function:

Involved in Mo-molybdopterin cofactor biosynthetic process

Specific function:

Involved in sulfur transfer in the conversion of molybdopterin precursor Z to molybdopterin

Gene Name:

moaD

Locus Tag:

PA3917

Molecular weight:

9.2 kDa

General function:

Involved in catalytic activity

Specific function:

Catalyzes the condensation of pantoate with beta-alanine in an ATP-dependent reaction via a pantoyl-adenylate intermediate

Gene Name:

panC

Locus Tag:

PA4730

Molecular weight:

30.8 kDa

Reactions

General function:

Involved in hydrolase activity

Specific function:

NAD(+) + H(2)O = AMP + NMN

Gene Name:

nudC

Locus Tag:

PA1823

Molecular weight:

31.4 kDa

Reactions

General function:

Involved in RNA processing

Specific function:

Catalyzes the conversion of 3'-phosphate to a 2',3'- cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps:(A) adenylation of the enzyme by ATP; (B) the enzyme acts on RNA-N3'P to produce RNA-N3'PP5'A; (C) a non catalytic nucleophilic attack by the adjacent 2'hydroxyl on the phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing

Gene Name:

rtcA

Locus Tag:

PA4585

Molecular weight:

36.6 kDa

Reactions

General function:

Involved in nucleotide binding

Specific function:

ATP + L-histidine + tRNA(His) = AMP + diphosphate + L-histidyl-tRNA(His)

Gene Name:

hisS

Locus Tag:

PA3802

Molecular weight:

47.5 kDa

Reactions

General function:

Involved in ATP binding

Specific function:

Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. This small ubiquitous enzyme involved in the energy metabolism and nucleotide synthesis, is essential for maintenance and cell growth

Gene Name:

adk

Locus Tag:

PA3686

Molecular weight:

23.1 kDa

Reactions

General function:

Involved in catalytic activity

Specific function:

Catalyzes the esterification, concomitant with transport, of exogenous long-chain fatty acids into metabolically active CoA thioesters for subsequent degradation or incorporation into phospholipids

Gene Name:

fadD

Locus Tag:

PA3299

Molecular weight:

61.7 kDa

Reactions

General function:

Involved in adenine phosphoribosyltransferase activity

Specific function:

Catalyzes a salvage reaction resulting in the formation of AMP, that is energically less costly than de novo synthesis

Gene Name:

apt

Locus Tag:

PA1543

Molecular weight:

19.8 kDa

Reactions

General function:

Involved in hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides

Specific function:

Acts on ADP-mannose and ADP-glucose as well as ADP- ribose. Prevents glycogen biosynthesis. The reaction catalyzed by this enzyme is a limiting step of the gluconeogenic process

Gene Name:

nudF

Locus Tag:

PA4971

Molecular weight:

23.1 kDa

Reactions

General function:

Involved in RNA binding

Specific function:

Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. This is a step in the synthesis of thiazole, in the thiamine biosynthesis pathway. The sulfur is donated as persulfide by iscS

Gene Name:

thiI

Locus Tag:

PA5118

Molecular weight:

54.8 kDa

Reactions

General function:

Involved in fatty acid biosynthetic process

Specific function:

Carrier of the growing fatty acid chain in fatty acid biosynthesis

Gene Name:

acpP

Locus Tag:

PA2966

Molecular weight:

8.7 kDa

General function:

threonylcarbamoyladenosine biosynthetic process

Specific function:

Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. Is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaE and TsaB. TsaD likely plays a direct catalytic role in this reaction. May also be involved in the metabolism of glycated proteins, but does not show sialoglycoprotease activity against glycophorin A.

Gene Name:

tsaD

Locus Tag:

PA0580

Molecular weight:

36.6 kDa

Reactions