Aspartyl-tRNA synthetase (PA0963)

Identification
Name: Aspartyl-tRNA synthetase
Synonyms:
  • Aspartate--tRNA ligase
  • AspRS
Gene Name: aspS
Enzyme Class:
Biological Properties
General Function: Involved in nucleotide binding
Specific Function: ATP + L-aspartate + tRNA(Asp) = AMP + diphosphate + L-aspartyl-tRNA(Asp)
Cellular Location: Cytoplasm
KEGG Pathways:
KEGG Reactions:
Thumb+Thumb+tRNA(Asp)+tRNA(Asp) ↔ Thumb+L-Aspartyl-tRNA(Asp)+Thumb+L-Aspartyl-tRNA(Asp)
L-Aspartic acid + Adenosine triphosphate + tRNA(Asp) + tRNA(Asp) ↔ Adenosine monophosphate + L-Aspartyl-tRNA(Asp) + Pyrophosphate + L-Aspartyl-tRNA(Asp)
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tRNA(Asp)+Thumb+Thumb ↔ L-Aspartyl-tRNA(Asp)+Thumb+Thumb
tRNA(Asp) + L-Aspartic acid + Adenosine triphosphate ↔ L-Aspartyl-tRNA(Asp) + Pyrophosphate + Adenosine monophosphate
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SMPDB Reactions:
L-Aspartic acid+Thumb+Thumb+tRNA(Asp)+Thumb → Pyrophosphate+Thumb+L-aspartyl-tRNA(Asp)
L-Aspartic acid + Adenosine triphosphate + Hydrogen ion + tRNA(Asp) + L-Aspartic acid → Pyrophosphate + Adenosine monophosphate + L-aspartyl-tRNA(Asp)
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PseudoCyc/BioCyc Reactions:
Thumb+Thumb+tRNA(Asp)+tRNA(Asp) ↔ Thumb+L-Aspartyl-tRNA(Asp)+Thumb+L-Aspartyl-tRNA(Asp)
L-Aspartic acid + Adenosine triphosphate + tRNA(Asp) + tRNA(Asp) ↔ Adenosine monophosphate + L-Aspartyl-tRNA(Asp) + Pyrophosphate + L-Aspartyl-tRNA(Asp)
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tRNA(Asp)+Thumb+Thumb ↔ L-Aspartyl-tRNA(Asp)+Thumb+Thumb
tRNA(Asp) + L-Aspartic acid + Adenosine triphosphate ↔ L-Aspartyl-tRNA(Asp) + Pyrophosphate + Adenosine monophosphate
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Thumb+Thumb+tRNA(Asp) → Thumb+Thumb+L-aspartyl-tRNA(Asp)
Adenosine triphosphate + L-Aspartic acid + tRNA(Asp) → Adenosine monophosphate + Pyrophosphate + L-aspartyl-tRNA(Asp)
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L-Aspartic acid+Thumb+Thumb+tRNA(Asp)+Thumb → Pyrophosphate+Thumb+L-aspartyl-tRNA(Asp)
L-Aspartic acid + Adenosine triphosphate + Hydrogen ion + tRNA(Asp) + L-Aspartic acid → Pyrophosphate + Adenosine monophosphate + L-aspartyl-tRNA(Asp)
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Thumb
tRNAasp + L-aspartate + ATP + H+ → L-aspartyl-tRNAasp + AMP + diphosphate
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Complex Reactions:
Thumb+Thumb+tRNA(Asp) → Thumb+Thumb+L-aspartyl-tRNA(Asp)
Adenosine triphosphate + L-Aspartic acid + tRNA(Asp) → Adenosine monophosphate + Pyrophosphate + L-aspartyl-tRNA(Asp)
ReactionCard
Transports: Not Available
Metabolites:
PAMDB IDNameView
PAMDB000014Adenosine monophosphateMetaboCard
PAMDB000148Adenosine triphosphateMetaboCard
PAMDB001633Hydrogen ionMetaboCard
PAMDB000080L-Aspartic acidMetaboCard
PAMDB000633PyrophosphateMetaboCard
GO Classification:
Component
cell part
cytoplasm
intracellular part
Function
adenyl nucleotide binding
adenyl ribonucleotide binding
aminoacyl-tRNA ligase activity
aspartate-tRNA ligase activity
ATP binding
binding
catalytic activity
ligase activity
ligase activity, forming aminoacyl-tRNA and related compounds
ligase activity, forming carbon-oxygen bonds
nucleic acid binding
nucleoside binding
nucleotide binding
purine nucleoside binding
Process
aspartyl-tRNA aminoacylation
biosynthetic process
cellular macromolecule biosynthetic process
cellular macromolecule metabolic process
macromolecule biosynthetic process
macromolecule metabolic process
metabolic process
ncRNA metabolic process
RNA metabolic process
translation
tRNA aminoacylation
tRNA aminoacylation for protein translation
tRNA metabolic process
Gene Properties
Locus tag: PA0963
Strand: +
Entrez Gene ID: 881917
Accession: NP_249654.1
GI: 15596160
Sequence start: 1048459
Sequence End: 1050234
Sequence Length: 1775
Gene Sequence: 
>PA0963
ATGATGCGCAGCCACTATTGCGGCCAACTGAACGAGAGCCTGGACGGCCAGGAAGTCACCCTTTGCGGTTGGGTTCACCGCCGCCGCGACCATGGTGGAGTGATCTTCCTCGATGTGCGCGATCGCGAGGGGCTGGCCCAGGTGGTGTTCGATCCGGACCGTGCGGAAACCTTCGCCAAGGCCGACCGGGTGCGCAGCGAGTTCGTGGTGAAGATCACCGGCAAGGTGCGCCTGCGCCCTGAAGGCGCGCGCAACCCGAACATGGCGTCTGGCTCCATCGAGGTACTGGGCTACGAACTGGAAGTGCTGAACCAGGCCGAGACTCCGCCGTTCCCGTTGGATGAATATTCCGATGTGGGCGAGGAAACGCGCCTGCGCTACCGCTTCATCGACCTGCGTCGTCCGGAGATGGCCGCCAAGCTGAAGCTGCGTGCGCGCATCACCAGCAGCATTCGCCGCTACCTGGACGACAACGGCTTCCTCGACGTCGAGACCCCGATCCTCGGCCGGCCGACGCCGGAAGGCGCGCGCGATTACCTGGTGCCGAGCCGTACCTACCCGGGCCACTTCTTCGCCCTGCCGCAGTCGCCACAGCTGTTCAAGCAACTGCTGATGGTCGCCGGCTTCGATCGCTACTACCAGATCGCCAAGTGCTTCCGTGACGAGGACCTGCGTGCCGACCGCCAGCCGGAATTCACCCAGATCGACATCGAGACCAGCTTCCTCGACGAGAGCGACATCATCGGCATCACCGAGAAGATGGTGCGCCAGCTGTTCAAGGAAGTGCTGGATGTCGAGTTCGACGAGTTCCCGCACATGCCGTTCGAAGAGGCCATGCGCCGCTACGGTTCGGACAAGCCCGACCTGCGCATCCCGCTGGAGTTGGTGGACGTCGCCGACCAGTTGAAGGAAGTGGAGTTCAAGGTCTTCTCCGGTCCGGCCAACGATCCGAAGGGGCGTGTCGCCGCCCTGCGTGTACCGGGCGCCGCCTCCATGCCGCGCAGCCAGATCGACGACTACACCAAGTTCGTCGGCATCTATGGTGCCAAGGGCCTGGCCTACATCAAGGTCAACGAGCGCGCCAAGGGCGTGGAAGGCCTGCAGTCGCCGATCGTGAAGTTCATCCCCGAGGCCAACCTGAACGTGATCCTCGATCGCGTTGGCGCGGTCGACGGCGACATCGTGTTCTTTGGCGCCGACAAGGCCAAGATCGTTTGCGATGCCCTGGGCGCGCTGCGTATCAAGGTCGGCCATGACCTCAAGCTGCTCACCCGGGAGTGGGCGCCCATGTGGGTCGTGGACTTCCCGATGTTCGAGGAGAACGACGACGGCTCGCTGTCCGCGCTGCACCACCCGTTCACCTCGCCCAAGTGCACCCCGGCTGAGCTTGAGGCCAATCCGGGCGCGGCACTTTCCCGCGCCTACGACATGGTGCTCAACGGTACCGAGCTGGGTGGCGGTTCCATCCGTATCCATGACAAGTCCATGCAGCAGGCGGTGTTCCGTGTGCTGGGCATCGACGAGGCGGAACAGGAAGAGAAATTCGGCTTCCTGCTCGACGCGCTCAAGTACGGTGCGCCGCCGCATGGCGGCCTGGCCTTCGGCCTGGATCGCCTGGTCATGCTGATGACCGGTGCATCGTCTATCCGCGAAGTGATCGCCTTCCCGAAGACCCAGAGCGCTGGCGACGTCATGACCCAGGCACCGGGTTCCGTGGACGGCAAGGCCCTGCGCGAGCTGCACATTCGCCTGCGCGAGCAGCCGAAGGCCGAGTGA
Protein Properties
Protein Residues: 591
Protein Molecular Weight: 66.2 kDa
Protein Theoretical pI: 5.21
Hydropathicity (GRAVY score): -0.297
Charge at pH 7 (predicted): -13.46
Protein Sequence: 
>PA0963
MMRSHYCGQLNESLDGQEVTLCGWVHRRRDHGGVIFLDVRDREGLAQVVFDPDRAETFAKADRVRSEFVVKITGKVRLRPEGARNPNMASGSIEVLGYELEVLNQAETPPFPLDEYSDVGEETRLRYRFIDLRRPEMAAKLKLRARITSSIRRYLDDNGFLDVETPILGRPTPEGARDYLVPSRTYPGHFFALPQSPQLFKQLLMVAGFDRYYQIAKCFRDEDLRADRQPEFTQIDIETSFLDESDIIGITEKMVRQLFKEVLDVEFDEFPHMPFEEAMRRYGSDKPDLRIPLELVDVADQLKEVEFKVFSGPANDPKGRVAALRVPGAASMPRSQIDDYTKFVGIYGAKGLAYIKVNERAKGVEGLQSPIVKFIPEANLNVILDRVGAVDGDIVFFGADKAKIVCDALGALRIKVGHDLKLLTREWAPMWVVDFPMFEENDDGSLSALHHPFTSPKCTPAELEANPGAALSRAYDMVLNGTELGGGSIRIHDKSMQQAVFRVLGIDEAEQEEKFGFLLDALKYGAPPHGGLAFGLDRLVMLMTGASSIREVIAFPKTQSAGDVMTQAPGSVDGKALRELHIRLREQPKAE
References
External Links:
Resource Link
Genome ID: PA0963
Entrez Gene ID: 881917
NCBI Protein ID: 15596160
General Reference: PaperBLAST - Find papers about PA0963 and its homologs