tRNA-specific 2-thiouridylase mnmA (PA2626)

Identification
Name: tRNA-specific 2-thiouridylase mnmA
Synonyms: Not Available
Gene Name: mnmA
Enzyme Class: Not Available
Biological Properties
General Function: Involved in sulfurtransferase activity
Specific Function: Catalyzes the 2-thiolation of uridine at the wobble position (U34) of tRNA(Lys), tRNA(Glu) and tRNA(Gln), leading to the formation of s(2)U34, the first step of tRNA-mnm(5)s(2)U34 synthesis. Sulfur is provided by iscS, via a sulfur-relay system. Binds ATP and its substrate tRNAs
Cellular Location: Cytoplasm
KEGG Pathways:
KEGG Reactions: Not Available
SMPDB Reactions: Not Available
PseudoCyc/BioCyc Reactions:
a tRNA uridine34+a [TusE sulfur carrier protein]-S-sulfanylcysteine+Thumb → a tRNA 2-thiouridine34+a [TusE sulfur carrier protein]-L-cysteine+Thumb+Thumb
a tRNA uridine34 + a [TusE sulfur carrier protein]-S-sulfanylcysteine + Adenosine triphosphate → a tRNA 2-thiouridine34 + a [TusE sulfur carrier protein]-L-cysteine + Adenosine monophosphate + Pyrophosphate
ReactionCard
Complex Reactions: Not Available
Transports: Not Available
Metabolites:
PAMDB IDNameView
PAMDB000014Adenosine monophosphateMetaboCard
PAMDB000148Adenosine triphosphateMetaboCard
PAMDB000633PyrophosphateMetaboCard
GO Classification:
Component
cell part
cytoplasm
intracellular part
Function
catalytic activity
transferase activity
Process
cellular macromolecule metabolic process
macromolecule metabolic process
metabolic process
ncRNA metabolic process
RNA metabolic process
tRNA metabolic process
tRNA processing
Gene Properties
Locus tag: PA2626
Strand: +
Entrez Gene ID: 882332
Accession: NP_251316.1
GI: 15597822
Sequence start: 2969987
Sequence End: 2971114
Sequence Length: 1127
Gene Sequence: 
>PA2626
ATGTCTGAATCCGCCCCCACCCCACGCCGCGAGCGCGTGATCGTCGGCATGTCCGGCGGCGTCGACTCGTCCGTCTCCGCCCTGCTCCTGTTGCAGCAGGGCTATCAGGTCGAAGGCCTGTTCATGAAGAACTGGGACGAGGACGACGGCACCGAATACTGTACCGCCCGCGAGGACCTGGCCGACGCCCAGGCCGTCTGCGACCGGATCGGCATCAAGCTGCACACGGCGAACTTCGCCGCGGAATACTGGGATAACGTGTTCGAGCACTTCCTTGCCGAGTACAAGGCCGGCCGTACGCCGAACCCGGACATCCTCTGCAACCGCGAAATCAAGTTCAAGGCGTTCCTCGACTACGCGCTGATGCTCGGCGCGGACCTGATCGCCACCGGCCACTATGTGCGGCGCCGCGACCGCGACGGGCGCACCGAGCTGCTCAAGGGCCTGGACCCGAACAAGGACCAGAGCTACTTCCTGCACGCGGTGGGCGGCGAGCAGATCGCCCGCAGCCTGTTCCCCGTCGGCGAACTGGAGAAGCCGGAAGTCCGCGCCATCGCCGAGAAGCATGGCCTCGCCACGGCGAAGAAGAAGGACTCCACGGGCATCTGCTTCATTGGCGAGCGACGCTTCACCGATTTCCTCAAGCAGTACCTGCCGGCCCAGCCGGGCGACATCGAGACCACCGAAGGCAAGGTCATCGGTCGCCACAGCGGCCTGATGTACCACACCATCGGCCAGCGCCAGGGCCTCGGCATCGGCGGTCTCAAGGAAGCCGGCGACGATCCCTGGTACGTGCTCGGCAAGGACCTGCAACGCAACGTGCTGCTGGTCGGCCAGGGCAACGACCACCCGCTGCTGTTCTCCCGCGCCCTGCTGGCCTCGCGAATCTACTGGGTCAACCCGGTCGAACTGGAGCGGCCGCGCCGCCTGCGGGCCAAGGTGCGCTACCGGCAGAGCGACCAGGACTGCGTCCTGGAGAAAACCGCCGAAGGCTACCGCGCGGTGTTCGACGAACCGCAGCGCGCAGTGACCCCGGGGCAATCGGTGGTGTTCTACGACGGCGACGTCTGCCTCGGCGGCGGCGTGATCGAAACGGCGGAAGCCTGGGATTTCGGAGGACGCCCGTGA
Protein Properties
Protein Residues: 375
Protein Molecular Weight: 41.9 kDa
Protein Theoretical pI: 5.92
Hydropathicity (GRAVY score): -0.419
Charge at pH 7 (predicted): -5.24
Protein Sequence: 
>PA2626
MSESAPTPRRERVIVGMSGGVDSSVSALLLLQQGYQVEGLFMKNWDEDDGTEYCTAREDLADAQAVCDRIGIKLHTANFAAEYWDNVFEHFLAEYKAGRTPNPDILCNREIKFKAFLDYALMLGADLIATGHYVRRRDRDGRTELLKGLDPNKDQSYFLHAVGGEQIARSLFPVGELEKPEVRAIAEKHGLATAKKKDSTGICFIGERRFTDFLKQYLPAQPGDIETTEGKVIGRHSGLMYHTIGQRQGLGIGGLKEAGDDPWYVLGKDLQRNVLLVGQGNDHPLLFSRALLASRIYWVNPVELERPRRLRAKVRYRQSDQDCVLEKTAEGYRAVFDEPQRAVTPGQSVVFYDGDVCLGGGVIETAEAWDFGGRP
References
External Links:
Resource Link
Genome ID: PA2626
Entrez Gene ID: 882332
NCBI Protein ID: 15597822
General Reference: PaperBLAST - Find papers about PA2626 and its homologs