Identification
Name: RNA 3'-terminal phosphate cyclase
Synonyms:
  • RNA cyclase
  • RNA-3'-phosphate cyclase
Gene Name: rtcA
Enzyme Class:
Biological Properties
General Function: Involved in RNA processing
Specific Function: Catalyzes the conversion of 3'-phosphate to a 2',3'- cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps:(A) adenylation of the enzyme by ATP; (B) the enzyme acts on RNA-N3'P to produce RNA-N3'PP5'A; (C) a non catalytic nucleophilic attack by the adjacent 2'hydroxyl on the phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing
Cellular Location: Cytoplasm
KEGG Pathways: Not Available
KEGG Reactions:
Thumb+RNA 3'-terminal-phosphateThumb+Thumb+RNA terminal-2',3'-cyclic-phosphate
Adenosine triphosphate + RNA 3'-terminal-phosphate ↔ Adenosine monophosphate + Pyrophosphate + RNA terminal-2',3'-cyclic-phosphate
ReactionCard
SMPDB Reactions: Not Available
PseudoCyc/BioCyc Reactions:
Thumb+RNA 3'-terminal-phosphateThumb+Thumb+RNA terminal-2',3'-cyclic-phosphate
Adenosine triphosphate + RNA 3'-terminal-phosphate → Adenosine monophosphate + Pyrophosphate + RNA terminal-2',3'-cyclic-phosphate
ReactionCard
Thumb+RNA 3'-terminal-phosphateThumb+Thumb+RNA terminal-2',3'-cyclic-phosphate
Adenosine triphosphate + RNA 3'-terminal-phosphate ↔ Adenosine monophosphate + Pyrophosphate + RNA terminal-2',3'-cyclic-phosphate
ReactionCard
Thumb
an RNA 3'-terminal-phosphate + an RNA 3'-terminal-phosphate + ATP → an RNA terminal-2',3'-cyclic-phosphate + an RNA terminal-2',3'-cyclic-phosphate + AMP + diphosphate
ReactionCard
Complex Reactions:
Thumb+RNA 3'-terminal-phosphateThumb+Thumb+RNA terminal-2',3'-cyclic-phosphate
Adenosine triphosphate + RNA 3'-terminal-phosphate → Adenosine monophosphate + Pyrophosphate + RNA terminal-2',3'-cyclic-phosphate
ReactionCard
Transports: Not Available
Metabolites:
PAMDB IDNameView
PAMDB000014Adenosine monophosphateMetaboCard
PAMDB000148Adenosine triphosphateMetaboCard
PAMDB000633PyrophosphateMetaboCard
GO Classification:
Component
cell part
cytoplasm
intracellular part
Function
catalytic activity
cyclase activity
ligase activity
ligase activity, forming phosphoric ester bonds
RNA-3'-phosphate cyclase activity
Process
cellular macromolecule metabolic process
macromolecule metabolic process
metabolic process
RNA metabolic process
RNA processing
Gene Properties
Locus tag: PA4585
Strand: +
Entrez Gene ID: 881010
Accession: NP_253275.1
GI: 15599781
Sequence start: 5134689
Sequence End: 5135714
Sequence Length: 1025
Gene Sequence:
>PA4585
ATGAGAAAGGACCTCATCGAACTCGACGGCAGCGAAGGCGGCGGGCAGATCCTGCGTAGCGCCCTGAGCCTGTCGATGACCAGCGGCCAGCCGCTGCGCATCCGCAACATCCGTGGCCGGCGCAGCCGTCCGGGGCTGCTGCGGCAGCACCTCACCGCGGTGCGCGCGGCGGCGGAAATCTGCGCGGCCGAGGTCGAGGGTGCCGAGCTCGGCTCACGCGAGCTGGCGTTCCGGCCCGGTGCGATCCGCGCCGGCGACTACGCCTTCGCCATCGGCTCCGCCGGCAGTTGCTCGCTGGTACTGCAAACCCTGCTGCCGGCGCTGCTCGCGGCCAACGGCGAAAGCCGGGTGAGGATCAGCGGCGGCACCCACAATCCACTGGCGCCGCCGGCGGACTTTCTCCGCGACAGCTGGCTGCCGCTGTTGCAGCGGATGGGCGCGGAAGTCGACCTGGAACTGCTGCGCCACGGCTTCGTACCCGCCGGCGGTGGCGAGCTGCTGGCGCGGGTACGCCCGGCACGCTGGCGGCCGCTGCAACTGGAGCATCCCGGCGCGGCGCTGCGGCGCCAGGCGCGGGCGCTGCTCGCCGGGATTCCCGGGCACGTCGGCGAGCGTGAGCTGGAGAGGGTCCGCCAGCGCCTCGGTTGGAGCGACGAAGAACGCCAGCTGGAGTTCCTCGCGGAGGACCAGGGGCCGGGCAACGCCCTGCTGCTGCGGATCGACTGCGAGCACATCTGCGCGACCTTCTGCGCCTTCGGCCAGGCCGGCGTCAGCGCCGAACGGGTGGCGGAACAGGTCGCGACGCAAGCCATCGGCTGGATGGAAAGCGGCTGCGCGGCGGACGAGCACCTCGCCGACCAATTGCTGCTGCCCATGGCCCTGGCCGGCGCCGGCAGCTTCACCACGCCGCGGCTGAGCGCCCATCTGCAAAGCAACCGCCGGGTTATCGAACGCTTCCTGCCGGTGAGGATCGGCGACCAGGCGCTGGACGGCGGCGGTCATCGCATCGTCATCACATCGGCTTGA
Protein Properties
Protein Residues: 341
Protein Molecular Weight: 36.6 kDa
Protein Theoretical pI: 7.89
Hydropathicity (GRAVY score): -0.088
Charge at pH 7 (predicted): 2.99
Protein Sequence:
>PA4585
MRKDLIELDGSEGGGQILRSALSLSMTSGQPLRIRNIRGRRSRPGLLRQHLTAVRAAAEICAAEVEGAELGSRELAFRPGAIRAGDYAFAIGSAGSCSLVLQTLLPALLAANGESRVRISGGTHNPLAPPADFLRDSWLPLLQRMGAEVDLELLRHGFVPAGGGELLARVRPARWRPLQLEHPGAALRRQARALLAGIPGHVGERELERVRQRLGWSDEERQLEFLAEDQGPGNALLLRIDCEHICATFCAFGQAGVSAERVAEQVATQAIGWMESGCAADEHLADQLLLPMALAGAGSFTTPRLSAHLQSNRRVIERFLPVRIGDQALDGGGHRIVITSA
References
External Links:
Resource Link
Genome ID: PA4585
Entrez Gene ID: 881010
NCBI Protein ID: 15599781
General Reference: PaperBLAST - Find papers about PA4585 and its homologs