DNA ligase (PA1529)

Identification
Name: DNA ligase
Synonyms:
  • Polydeoxyribonucleotide synthase [NAD+]
Gene Name: ligA
Enzyme Class:
Biological Properties
General Function: Involved in DNA ligase (NAD+) activity
Specific Function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA
Cellular Location: Cytoplasmic
KEGG Pathways:
KEGG Reactions:
Thumb+Thumb ↔ Thumb+2 Thumb+Thumb+Thumb
Water + NAD ↔ Adenosine monophosphate + 2 Hydrogen ion + Nicotinamide ribotide + NMN
ReactionCard
Thumb+DNA ↔ Thumb+Thumb
NAD + DNA ↔ Adenosine monophosphate + NMN
ReactionCard
SMPDB Reactions: Not Available
PseudoCyc/BioCyc Reactions:
Thumb+Thumb ↔ Thumb+2 Thumb+Thumb+Thumb
Water + NAD ↔ Adenosine monophosphate + 2 Hydrogen ion + Nicotinamide ribotide + NMN
ReactionCard
DNAn+(deoxynucleotides)(m)+Thumb → (deoxynucleotides)(m)+Thumb+Thumb
DNAn + (deoxynucleotides)(m) + NAD → (deoxynucleotides)(m) + Nicotinamide ribotide + Adenosine monophosphate
ReactionCard
Complex Reactions:
Thumb+(deoxyribonucleotide)(n)+(deoxyribonucleotide)(m) → Thumb+Thumb+(deoxyribonucleotide)(n+m)
NAD + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) → Adenosine monophosphate + NMN + (deoxyribonucleotide)(n+m)
ReactionCard
Transports: Not Available
Metabolites:
PAMDB IDNameView
PAMDB000014Adenosine monophosphateMetaboCard
PAMDB001633Hydrogen ionMetaboCard
PAMDB000197NADMetaboCard
PAMDB000095Nicotinamide ribotideMetaboCard
PAMDB003612NMNMetaboCard
PAMDB000142WaterMetaboCard
GO Classification:
Component
cell part
intracellular
Function
binding
catalytic activity
DNA binding
DNA ligase (NAD+) activity
DNA ligase activity
ligase activity
ligase activity, forming phosphoric ester bonds
nucleic acid binding
Process
cellular macromolecule metabolic process
DNA metabolic process
DNA repair
DNA replication
macromolecule metabolic process
metabolic process
Gene Properties
Locus tag: PA1529
Strand: +
Entrez Gene ID: 879269
Accession: NP_250220.1
GI: 15596726
Sequence start: 1666025
Sequence End: 1668409
Sequence Length: 2384
Gene Sequence: 
>PA1529
ATGACCGACACCCAGGCTGCCGCCGAACGCATCGCGCAACTGCGAACCGAACTGGACACCCACAATTACCGTTACTACGTGCTCGACGAACCGAGCATTCCCGACGCCGAATACGATCGCCTGTTCCGCGAGTTGCAGGCGCTGGAGGCCGAGTACCCGCAACTGCTGACGCCCGACTCGCCGACCCAGCGGGTCAGCGGCACGCCGGCCTCGGCGTTCGGCGAAGTGCGCCATGAGATCCCCATGCTCAGCCTCGGCAACGCCTTCGAGGAGCAGGACCTGCTGGACTTCGACCGCCGCGTACGCGAAGGACTGGCCGACCTGTTGCCTGGCGGCGACCTGCTCGGCGGTGGCGCGGAGGTCGAGTACAGTTGCGAGCCGAAGCTGGACGGCCTGGCCGTCAGCCTGCTCTACGAACGCGGCCAACTGGTGCGCGGCGCCACCCGTGGCGATGGCAGCACCGGCGAGGACATCACCAGCAACGTACGCACCATTCGCAACGTACCGCTGAAGCTGCACGGCGAAGGCTGGCCGGAGATCCTCGAAGTGCGCGGCGAGGTGTTCATGTCCAAGGCCGGTTTCGAGGCGCTCAACGCCAAGGCCGTGGAAACCGGCGGCAAAACCTTCGCCAACCCGCGCAACGCGGCGGCCGGCAGCCTGCGCCAGCTGGATTCAAAGATCACCGCCAGTCGCCCGCTGGAATTCTGTGCCTACGGCTTCGGCCAGGTCAGCGGGACGCTGCCGGACACCCAGGTGGGCATCCTCGAGGCGTTCCGCGGCTGGGGTATCCCCATCAGTCGCGAGCTGCGCCTGGTCAAGGGCGCCCAGGCCTGCCGCGACTACTACGACGACATCGGTCGCCGACGCGATGCGCTGGCCTACGAGATCGACGGCGTGGTGTTCAAGGTCAACCGCATCGCCTTCCAGCGCGAACTGGGCTTCCGCGCGCGCGAGCCGCGTTGGGCGATCGCCCACAAGTTCCCCGCGCGCGAGGAGTTGACCGAGCTGCTGGGTGTCGAGTTCCAGGTCGGCCGCACCGGCGCCGTGACCCCGGTGGCACGCCTCAAGCCGGTGCAGGTGGCCGGCGTCACGGTGTCCAATGCGACCCTGCACAACATGGATGAAGTCGCCCGCCTGGGCCTGCGCATCGGTGATACGGTGGTGATCCGCCGTGCTGGCGATGTGATTCCGCAGGTCATGCAGGTGGTGCTCGAGCGCCGCCCGGCGGATGCGCAGGCTATCGAGGTGCCCGAGCATTGCCCGGTCTGCGGCTCGGCCGTGGAGCGGACCCAGTTGGTCAAGCGCAGCAAGGGCAAGGAGTCGATCAGCGAGGGCGCCATCTATCGCTGCGTCGGCCGCCTCTCCTGTCAGGCGCAGTTGAAGCAGGCGATCATCCATTTCGTATCGCGCCGGGCAATGGATATCGACGGTCTTGGCGACAAGATCGTCGAGCAACTGGTGGATCGCGGGCTGGTCGCCTCGCCGGCGGATCTCTATACCCTGACCTATGAACAGGTGTTCGAGCTGGAAGGGTTCGCCGAGCTGTCCACCAACAACCTGCTGACGGCTATCGCCGATAGCCGCAAGCCTTCGCTGGCGCGCTTCATCTTCGCTCTGGGGATTCCCGACGTCGGCGAGGAAACCGCCAAGCTGCTGGCGCGTTCGCTGGGTTCGCTCGAGCGGATCGGCAAGGCGCTCCCGGAAGTGTTGACCTACCTGCCGGACGTCGGCGCCGAGGTAGCCTACGAGATCCACAACTTCTTCGCCGACGAGCACAACCGCCAGGTGATCGCCCAGTTGCGCGATGCCGAACATGGCGTGCAGTTGCAGGAAGAGGGCGAGGTCGCCGCGGAGTTCGCCGCCTGTGCCTCGCTGGCCGGTTTCATCGACAAGCTGAACATTCCCTTCATCGCCGCCACCGGCGCGGAAAAGCTGGCTAGCCGCTTCGGTTCGCTCGACGGCATCATCCGTGCCGACTGGCTCGATCTGCGCCAGGTGGAGCGTCTGCCCGAGCGAGCGGCGAAAAGCCTGCGCGATTTCTTCGACGAGCCGGCCAACGTGCAGCGTGCCCTGGCCATCGAGGCGCAGTTGCGTGAGTTCGGCATGCATTGGCAGAGCGAGCGCAAGGCGGTCGAAGGCCTGCCTCTGGCCGGGCAGACCTGGGTGCTGACCGGCACCCTGGAGGCGATGAGCCGGGACGTGGCCAAGGACAAGCTGGAAGGGCTCGGCGCCAAGGTGGCCGGCTCGGTGTCGGCCAAGACCCACTGCGTGGTCGCCGGGCCCGGCGCCGGCTCGAAGCTGGCCAAGGCCAACGAGCTGGGCGTGAAGGTGCTCGACGAAGACGGCCTGCTGAAACTGTTCGACGAGCACGGCGTCGCCCGCTGA
Protein Properties
Protein Residues: 794
Protein Molecular Weight: 86.8 kDa
Protein Theoretical pI: 5.08
Hydropathicity (GRAVY score): -0.177
Charge at pH 7 (predicted): -19.07
Protein Sequence: 
>PA1529
MTDTQAAAERIAQLRTELDTHNYRYYVLDEPSIPDAEYDRLFRELQALEAEYPQLLTPDSPTQRVSGTPASAFGEVRHEIPMLSLGNAFEEQDLLDFDRRVREGLADLLPGGDLLGGGAEVEYSCEPKLDGLAVSLLYERGQLVRGATRGDGSTGEDITSNVRTIRNVPLKLHGEGWPEILEVRGEVFMSKAGFEALNAKAVETGGKTFANPRNAAAGSLRQLDSKITASRPLEFCAYGFGQVSGTLPDTQVGILEAFRGWGIPISRELRLVKGAQACRDYYDDIGRRRDALAYEIDGVVFKVNRIAFQRELGFRAREPRWAIAHKFPAREELTELLGVEFQVGRTGAVTPVARLKPVQVAGVTVSNATLHNMDEVARLGLRIGDTVVIRRAGDVIPQVMQVVLERRPADAQAIEVPEHCPVCGSAVERTQLVKRSKGKESISEGAIYRCVGRLSCQAQLKQAIIHFVSRRAMDIDGLGDKIVEQLVDRGLVASPADLYTLTYEQVFELEGFAELSTNNLLTAIADSRKPSLARFIFALGIPDVGEETAKLLARSLGSLERIGKALPEVLTYLPDVGAEVAYEIHNFFADEHNRQVIAQLRDAEHGVQLQEEGEVAAEFAACASLAGFIDKLNIPFIAATGAEKLASRFGSLDGIIRADWLDLRQVERLPERAAKSLRDFFDEPANVQRALAIEAQLREFGMHWQSERKAVEGLPLAGQTWVLTGTLEAMSRDVAKDKLEGLGAKVAGSVSAKTHCVVAGPGAGSKLAKANELGVKVLDEDGLLKLFDEHGVAR
References
External Links:
Resource Link
Genome ID: PA1529
Entrez Gene ID: 879269
NCBI Protein ID: 15596726
General Reference: PaperBLAST - Find papers about PA1529 and its homologs