Identification
Name: Isoleucyl-tRNA synthetase
Synonyms:
  • Isoleucine--tRNA ligase
  • IleRS
Gene Name: ileS
Enzyme Class:
Biological Properties
General Function: Involved in nucleotide binding
Specific Function: Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile)
Cellular Location: Cytoplasm
KEGG Pathways:
  • Aminoacyl-tRNA biosynthesis pae00970
  • Valine, leucine and isoleucine biosynthesis pae00290
KEGG Reactions:
Thumb+Thumb+tRNA(Ile)+tRNA(Ile)Thumb+L-Isoleucyl-tRNA(Ile)+Thumb+L-Isoleucyl-tRNA(Ile)
Adenosine triphosphate + L-Isoleucine + tRNA(Ile) + tRNA(Ile) ↔ Adenosine monophosphate + L-Isoleucyl-tRNA(Ile) + Pyrophosphate + L-Isoleucyl-tRNA(Ile)
ReactionCard
Thumb+Thumb+tRNA(Ile)Thumb+Thumb+L-Isoleucyl-tRNA(Ile)
SMPDB Reactions:
L-Isoleucine+Thumb+Thumb+tRNA(Ile)+ThumbL-Isoleucyl-tRNA(Ile)+Thumb+Pyrophosphate
L-Isoleucine + Adenosine triphosphate + Hydrogen ion + tRNA(Ile) + L-Isoleucine → L-Isoleucyl-tRNA(Ile) + Adenosine monophosphate + Pyrophosphate
ReactionCard
PseudoCyc/BioCyc Reactions:
Thumb+Thumb+tRNA(Ile)+tRNA(Ile)Thumb+L-Isoleucyl-tRNA(Ile)+Thumb+L-Isoleucyl-tRNA(Ile)
Adenosine triphosphate + L-Isoleucine + tRNA(Ile) + tRNA(Ile) ↔ Adenosine monophosphate + L-Isoleucyl-tRNA(Ile) + Pyrophosphate + L-Isoleucyl-tRNA(Ile)
ReactionCard
Thumb+Thumb+tRNA(Ile)Thumb+Thumb+L-Isoleucyl-tRNA(Ile)
Thumb+Thumb+tRNA(Ile)Thumb+Thumb+L-isoleucyl-tRNA(Ile)
L-Isoleucine+Thumb+Thumb+tRNA(Ile)+ThumbL-Isoleucyl-tRNA(Ile)+Thumb+Pyrophosphate
L-Isoleucine + Adenosine triphosphate + Hydrogen ion + tRNA(Ile) + L-Isoleucine → L-Isoleucyl-tRNA(Ile) + Adenosine monophosphate + Pyrophosphate
ReactionCard
Thumb
a tRNAile + L-isoleucine + ATP + H+ → an L-isoleucyl-[tRNAile] + AMP + diphosphate
ReactionCard
Complex Reactions:
Thumb+Thumb+tRNA(Ile)Thumb+Thumb+L-isoleucyl-tRNA(Ile)
Transports: Not Available
Metabolites:
PAMDB IDNameView
PAMDB000014Adenosine monophosphateMetaboCard
PAMDB000148Adenosine triphosphateMetaboCard
PAMDB001633Hydrogen ionMetaboCard
PAMDB000071L-IsoleucineMetaboCard
PAMDB000633PyrophosphateMetaboCard
GO Classification:
Component
cell part
cytoplasm
intracellular part
Function
adenyl nucleotide binding
adenyl ribonucleotide binding
aminoacyl-tRNA ligase activity
ATP binding
binding
catalytic activity
isoleucine-tRNA ligase activity
ligase activity
ligase activity, forming aminoacyl-tRNA and related compounds
ligase activity, forming carbon-oxygen bonds
nucleoside binding
nucleotide binding
purine nucleoside binding
Process
biosynthetic process
cellular macromolecule biosynthetic process
cellular macromolecule metabolic process
isoleucyl-tRNA aminoacylation
macromolecule biosynthetic process
macromolecule metabolic process
metabolic process
ncRNA metabolic process
RNA metabolic process
translation
tRNA aminoacylation
tRNA aminoacylation for protein translation
tRNA metabolic process
Gene Properties
Locus tag: PA4560
Strand: -
Entrez Gene ID: 878194
Accession: NP_253250.1
GI: 15599756
Sequence start: 5106951
Sequence End: 5109782
Sequence Length: 2831
Gene Sequence:
>PA4560
ATGACCGACTACAAAGCGACGCTAAACCTTCCGGAAACCGCCTTCCCAATGAAAGCCGGCCTGCCCCAGCGCGAGCCGGAAACCCTGAAGTTCTGGAACGACATCGGTCTGTACCAGAAGCTGCGGGCCATTGGCGGCGACCGTCCGAAGTTCGTCCTGCACGATGGCCCTCCGTACGCCAACGGCAGCATTCACATCGGTCACGCGGTCAACAAGATTCTCAAGGACATCATTGTCCGCTCCAAGACCCTGGCGGGCTACGACGCGCCCTATGTCCCGGGCTGGGATTGCCACGGCCTGCCCATCGAGCACAAGGTCGAGACCACCCACGGCAAGAATCTTCCGGCGGACAAGACCCGCGAACTGTGCCGCGAATACGCGGCCGAGCAGATCGAAGGGCAGAAGGCCGACTTCATTCGCCTGGGCGTGCTCGGCGAGTGGGACAACCCCTACAAGACCATGAATTTCGCCAACGAGGCGAACGAAATCCGCGCCCTCGCGGAAATGGTCAAGCAGGACTTCGTGTTCAAGGGCCTGAAGCCGGTCAACTGGTGCTTCGACTGTGGTTCGGCGCTGGCCGAGGCCGAGGTCGAGTACGCCGACAAGAAGTCGCCGACCATCGACGTCGGCTTCCCGGTGGCGGATGCCGACAAGCTGGCCGCGGCCTTCGGCCTGGCGGCCCTGGACAAGCCGGCGCAGATCGTCATCTGGACCACCACGCCCTGGACCATCCCGGCCAACCAGGCGCTGAACGTGCATCCGGAGATCGACTACGCGCTGGTCGATGCCGGCGATCGCTACCTGGTGCTGGCCGAGGCCCTGGTCGAGTCCTGCCTGGCGCGCTACCAGCGCGAGGGCAAGGTGGTCGCCACCGCCAAGGGCGAGGCGCTGGAACTGATCAACTTCCGCCATCCGTTCTACGAGCGCCTGTCGCCGGTCTACCTGGCCGACTACGTGGCCCTCGACGCCGGCACCGGTATCGTCCACTCATCGCCGGCCTACGGCGAAGACGACTTCTACACCTGCAAGCGCTACGGGATGAGCAACGACGACATCCTCAGCCCGGTGCAGAGCAACGGCGTCTACGTCGACTCGTTGCCGTTCTTCGGCGGTCAGTTCATCTGGAAGGCCAACCCCAACGTGGTGGCCAAGCTGGAAGAGGTCGGCAGCCTGCTGGCCCACGAAACCATCAATCACAGCTATATGCACTGCTGGCGGCACAAGACGCCGCTGATCTATCGCGCCACCGCGCAGTGGTTCGTCGGCATGGACAAGCAGCCCAGGCAGGGTGCTTCCCTGCGCGAGCGAGCGCTGGAAGCCATCACCCAGACCGAATTCGTCCCGGGCTGGGGCCAGGCGCGTCTGCACGGAATGATCGCCGGGCGTCCCGACTGGTGCATCTCGCGCCAGCGCAACTGGGGCGTACCGATCCCGTTCTTCCTGCACAAGGCCAGTGGCGAACTGCATCCACGCACCGTGGAGCTGATGGAAGAGGTCGCGCAGCGAGTCGAGAAGGAAGGCATCGAAGCCTGGTTCAAGCTCGACGCCGCCGAGCTGCTCGGCGAGGAGGCCGCGCAGTACGAGAAGATCAACGACACCCTGGACGTCTGGTTCGACTCCGGCACCACCCATTGGCACGTGCTGCGTGGTTCCCACCGGATCGGCCATGCCAGCGGCCCGGTCGCCGATCTCTACCTGGAAGGTTCCGACCAGCACCGCGGCTGGTTCCACTCCTCGCTGCTGACCGGCTGCGCGATCGACAACCATGCGCCGTACCGCCAGTTGCTGACCCACGGTTTCACCGTCGACGAGTCCGGCCGCAAGATGTCCAAGTCGCTGGGCAACACCGTGGTGCCGCAGACCGTCATCGATACCCTGGGCGCCGACATCCTGCGCCTGTGGGTGGCTTCGACCGATTACTCGGGCGAGATCGCGGTATCCCAGCAGATTCTCCAGCGTAGCGCCGATGCCTACCGGCGGATCCGCAATACCACGCGCTTCCTGCTGTCCAACCTGAACGGTTTCGATCCGGCCACGGATCTGCTGCCGCCCCAGGAAATGCTGGCCCTGGATCGCTGGGCGGTCGATCGCGCCCTGCTCTTGCAGCGCGAGATCGAAGAGGCGTACCGCGAGTACCGCTTCTGGAACGTCTATTCCAAGGTGCACAACTTCTGCGTACAGGAGCTGGGCGGGTTCTACTTGGACATCATCAAGGACCGCCAGTACACCACCGGCGCCAACAGCGTCGCCCGGCGCTCCTGCCAGACCGCACTGTTCCACATCGCCGAGGCCCTCGTACGCTGGATCGCCCCGATCCTGGCGTTCACCGCCGAGGAAGTCTGGAAGTTCCTGCCCGGCGAGCGCGCCGAGTCGGTAATGCTCGCCACCTGGTACGACGGCCTCAACGAACTGCCGGCCGACGTGACACTGAATCGCCAGTACTGGGAACAGGTCATGGCGGTGAAGGCCGCGGTCAACAAGGAGCTGGAGAACCAGCGCGCGGCCAAGGCCGTGGGCGGCAACCTGCAGGCCGAGGTCACCCTGTATGCGGAAGACGCGCTGCAGGCCCAATTGGCCAAGCTGGGCAACGAGCTACGCTTCGTCCTGATCACCTCGACCGCGACCCTGGCGCCGCTGTCGGCGGCTCCGGCCGACGCGGTGGACAGCGAAGTCGCCGGCCTCAAGCTGAAAGTGGTCAAGTCGACCCACGCCAAGTGCGGTCGTTGCTGGCACCATCGCGAGGACGTCGGTCAGCACGCTGCGCATCCTGACCTGTGCGGTCGCTGCATCGAGAACATCGAAGGCAGCGGCGAGGTTCGCCACTATGCCTGA
Protein Properties
Protein Residues: 943
Protein Molecular Weight: 105.5 kDa
Protein Theoretical pI: 6.09
Hydropathicity (GRAVY score): -0.265
Charge at pH 7 (predicted): -16.7
Protein Sequence:
>PA4560
MTDYKATLNLPETAFPMKAGLPQREPETLKFWNDIGLYQKLRAIGGDRPKFVLHDGPPYANGSIHIGHAVNKILKDIIVRSKTLAGYDAPYVPGWDCHGLPIEHKVETTHGKNLPADKTRELCREYAAEQIEGQKADFIRLGVLGEWDNPYKTMNFANEANEIRALAEMVKQDFVFKGLKPVNWCFDCGSALAEAEVEYADKKSPTIDVGFPVADADKLAAAFGLAALDKPAQIVIWTTTPWTIPANQALNVHPEIDYALVDAGDRYLVLAEALVESCLARYQREGKVVATAKGEALELINFRHPFYERLSPVYLADYVALDAGTGIVHSSPAYGEDDFYTCKRYGMSNDDILSPVQSNGVYVDSLPFFGGQFIWKANPNVVAKLEEVGSLLAHETINHSYMHCWRHKTPLIYRATAQWFVGMDKQPRQGASLRERALEAITQTEFVPGWGQARLHGMIAGRPDWCISRQRNWGVPIPFFLHKASGELHPRTVELMEEVAQRVEKEGIEAWFKLDAAELLGEEAAQYEKINDTLDVWFDSGTTHWHVLRGSHRIGHASGPVADLYLEGSDQHRGWFHSSLLTGCAIDNHAPYRQLLTHGFTVDESGRKMSKSLGNTVVPQTVIDTLGADILRLWVASTDYSGEIAVSQQILQRSADAYRRIRNTTRFLLSNLNGFDPATDLLPPQEMLALDRWAVDRALLLQREIEEAYREYRFWNVYSKVHNFCVQELGGFYLDIIKDRQYTTGANSVARRSCQTALFHIAEALVRWIAPILAFTAEEVWKFLPGERAESVMLATWYDGLNELPADVTLNRQYWEQVMAVKAAVNKELENQRAAKAVGGNLQAEVTLYAEDALQAQLAKLGNELRFVLITSTATLAPLSAAPADAVDSEVAGLKLKVVKSTHAKCGRCWHHREDVGQHAAHPDLCGRCIENIEGSGEVRHYA
References
External Links:
Resource Link
Genome ID: PA4560
Entrez Gene ID: 878194
NCBI Protein ID: 15599756
General Reference: PaperBLAST - Find papers about PA4560 and its homologs