| Identification |
| Name: |
Isoleucyl-tRNA synthetase |
| Synonyms: |
- Isoleucine--tRNA ligase
- IleRS
|
| Gene Name: |
ileS |
| Enzyme Class: |
|
| Biological Properties |
| General Function: |
Involved in nucleotide binding |
| Specific Function: |
Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) |
| Cellular Location: |
Cytoplasm |
| KEGG Pathways: |
- Aminoacyl-tRNA biosynthesis pae00970
- Valine, leucine and isoleucine biosynthesis pae00290
|
| KEGG Reactions: |
|
 | + |  | + | tRNA(Ile) | + | tRNA(Ile) | ↔ |  | + | L-Isoleucyl-tRNA(Ile) | + |  | + | L-Isoleucyl-tRNA(Ile) |
| | |
| + | | + | tRNA(Ile) | ↔ | | + | | + | L-Isoleucyl-tRNA(Ile) |
| |
|
| SMPDB Reactions: |
|
L-Isoleucine | + | | + |  | + | tRNA(Ile) | + | | → | L-Isoleucyl-tRNA(Ile) | + | | + | Pyrophosphate |
| |
|
| PseudoCyc/BioCyc Reactions: |
|
| Complex Reactions: |
|
| + | | + | tRNA(Ile) | → | | + | | + | L-isoleucyl-tRNA(Ile) |
| |
|
| Transports: |
Not Available |
| Metabolites: |
|
| GO Classification: |
| Component |
|---|
| cell part | | cytoplasm | | intracellular part | | Function |
|---|
| adenyl nucleotide binding | | adenyl ribonucleotide binding | | aminoacyl-tRNA ligase activity | | ATP binding | | binding | | catalytic activity | | isoleucine-tRNA ligase activity | | ligase activity | | ligase activity, forming aminoacyl-tRNA and related compounds | | ligase activity, forming carbon-oxygen bonds | | nucleoside binding | | nucleotide binding | | purine nucleoside binding | | Process |
|---|
| biosynthetic process | | cellular macromolecule biosynthetic process | | cellular macromolecule metabolic process | | isoleucyl-tRNA aminoacylation | | macromolecule biosynthetic process | | macromolecule metabolic process | | metabolic process | | ncRNA metabolic process | | RNA metabolic process | | translation | | tRNA aminoacylation | | tRNA aminoacylation for protein translation | | tRNA metabolic process |
|
| Gene Properties |
| Locus tag: |
PA4560 |
| Strand: |
- |
| Entrez Gene ID: |
878194 |
| Accession: |
NP_253250.1 |
| GI: |
15599756 |
| Sequence start: |
5106951 |
| Sequence End: |
5109782 |
| Sequence Length: |
2831 |
| Gene Sequence: |
>PA4560
ATGACCGACTACAAAGCGACGCTAAACCTTCCGGAAACCGCCTTCCCAATGAAAGCCGGCCTGCCCCAGCGCGAGCCGGAAACCCTGAAGTTCTGGAACGACATCGGTCTGTACCAGAAGCTGCGGGCCATTGGCGGCGACCGTCCGAAGTTCGTCCTGCACGATGGCCCTCCGTACGCCAACGGCAGCATTCACATCGGTCACGCGGTCAACAAGATTCTCAAGGACATCATTGTCCGCTCCAAGACCCTGGCGGGCTACGACGCGCCCTATGTCCCGGGCTGGGATTGCCACGGCCTGCCCATCGAGCACAAGGTCGAGACCACCCACGGCAAGAATCTTCCGGCGGACAAGACCCGCGAACTGTGCCGCGAATACGCGGCCGAGCAGATCGAAGGGCAGAAGGCCGACTTCATTCGCCTGGGCGTGCTCGGCGAGTGGGACAACCCCTACAAGACCATGAATTTCGCCAACGAGGCGAACGAAATCCGCGCCCTCGCGGAAATGGTCAAGCAGGACTTCGTGTTCAAGGGCCTGAAGCCGGTCAACTGGTGCTTCGACTGTGGTTCGGCGCTGGCCGAGGCCGAGGTCGAGTACGCCGACAAGAAGTCGCCGACCATCGACGTCGGCTTCCCGGTGGCGGATGCCGACAAGCTGGCCGCGGCCTTCGGCCTGGCGGCCCTGGACAAGCCGGCGCAGATCGTCATCTGGACCACCACGCCCTGGACCATCCCGGCCAACCAGGCGCTGAACGTGCATCCGGAGATCGACTACGCGCTGGTCGATGCCGGCGATCGCTACCTGGTGCTGGCCGAGGCCCTGGTCGAGTCCTGCCTGGCGCGCTACCAGCGCGAGGGCAAGGTGGTCGCCACCGCCAAGGGCGAGGCGCTGGAACTGATCAACTTCCGCCATCCGTTCTACGAGCGCCTGTCGCCGGTCTACCTGGCCGACTACGTGGCCCTCGACGCCGGCACCGGTATCGTCCACTCATCGCCGGCCTACGGCGAAGACGACTTCTACACCTGCAAGCGCTACGGGATGAGCAACGACGACATCCTCAGCCCGGTGCAGAGCAACGGCGTCTACGTCGACTCGTTGCCGTTCTTCGGCGGTCAGTTCATCTGGAAGGCCAACCCCAACGTGGTGGCCAAGCTGGAAGAGGTCGGCAGCCTGCTGGCCCACGAAACCATCAATCACAGCTATATGCACTGCTGGCGGCACAAGACGCCGCTGATCTATCGCGCCACCGCGCAGTGGTTCGTCGGCATGGACAAGCAGCCCAGGCAGGGTGCTTCCCTGCGCGAGCGAGCGCTGGAAGCCATCACCCAGACCGAATTCGTCCCGGGCTGGGGCCAGGCGCGTCTGCACGGAATGATCGCCGGGCGTCCCGACTGGTGCATCTCGCGCCAGCGCAACTGGGGCGTACCGATCCCGTTCTTCCTGCACAAGGCCAGTGGCGAACTGCATCCACGCACCGTGGAGCTGATGGAAGAGGTCGCGCAGCGAGTCGAGAAGGAAGGCATCGAAGCCTGGTTCAAGCTCGACGCCGCCGAGCTGCTCGGCGAGGAGGCCGCGCAGTACGAGAAGATCAACGACACCCTGGACGTCTGGTTCGACTCCGGCACCACCCATTGGCACGTGCTGCGTGGTTCCCACCGGATCGGCCATGCCAGCGGCCCGGTCGCCGATCTCTACCTGGAAGGTTCCGACCAGCACCGCGGCTGGTTCCACTCCTCGCTGCTGACCGGCTGCGCGATCGACAACCATGCGCCGTACCGCCAGTTGCTGACCCACGGTTTCACCGTCGACGAGTCCGGCCGCAAGATGTCCAAGTCGCTGGGCAACACCGTGGTGCCGCAGACCGTCATCGATACCCTGGGCGCCGACATCCTGCGCCTGTGGGTGGCTTCGACCGATTACTCGGGCGAGATCGCGGTATCCCAGCAGATTCTCCAGCGTAGCGCCGATGCCTACCGGCGGATCCGCAATACCACGCGCTTCCTGCTGTCCAACCTGAACGGTTTCGATCCGGCCACGGATCTGCTGCCGCCCCAGGAAATGCTGGCCCTGGATCGCTGGGCGGTCGATCGCGCCCTGCTCTTGCAGCGCGAGATCGAAGAGGCGTACCGCGAGTACCGCTTCTGGAACGTCTATTCCAAGGTGCACAACTTCTGCGTACAGGAGCTGGGCGGGTTCTACTTGGACATCATCAAGGACCGCCAGTACACCACCGGCGCCAACAGCGTCGCCCGGCGCTCCTGCCAGACCGCACTGTTCCACATCGCCGAGGCCCTCGTACGCTGGATCGCCCCGATCCTGGCGTTCACCGCCGAGGAAGTCTGGAAGTTCCTGCCCGGCGAGCGCGCCGAGTCGGTAATGCTCGCCACCTGGTACGACGGCCTCAACGAACTGCCGGCCGACGTGACACTGAATCGCCAGTACTGGGAACAGGTCATGGCGGTGAAGGCCGCGGTCAACAAGGAGCTGGAGAACCAGCGCGCGGCCAAGGCCGTGGGCGGCAACCTGCAGGCCGAGGTCACCCTGTATGCGGAAGACGCGCTGCAGGCCCAATTGGCCAAGCTGGGCAACGAGCTACGCTTCGTCCTGATCACCTCGACCGCGACCCTGGCGCCGCTGTCGGCGGCTCCGGCCGACGCGGTGGACAGCGAAGTCGCCGGCCTCAAGCTGAAAGTGGTCAAGTCGACCCACGCCAAGTGCGGTCGTTGCTGGCACCATCGCGAGGACGTCGGTCAGCACGCTGCGCATCCTGACCTGTGCGGTCGCTGCATCGAGAACATCGAAGGCAGCGGCGAGGTTCGCCACTATGCCTGA |
| Protein Properties |
| Protein Residues: |
943 |
| Protein Molecular Weight: |
105.5 kDa |
| Protein Theoretical pI: |
6.09 |
| Hydropathicity (GRAVY score): |
-0.265 |
| Charge at pH 7 (predicted): |
-16.7 |
| Protein Sequence: |
>PA4560
MTDYKATLNLPETAFPMKAGLPQREPETLKFWNDIGLYQKLRAIGGDRPKFVLHDGPPYANGSIHIGHAVNKILKDIIVRSKTLAGYDAPYVPGWDCHGLPIEHKVETTHGKNLPADKTRELCREYAAEQIEGQKADFIRLGVLGEWDNPYKTMNFANEANEIRALAEMVKQDFVFKGLKPVNWCFDCGSALAEAEVEYADKKSPTIDVGFPVADADKLAAAFGLAALDKPAQIVIWTTTPWTIPANQALNVHPEIDYALVDAGDRYLVLAEALVESCLARYQREGKVVATAKGEALELINFRHPFYERLSPVYLADYVALDAGTGIVHSSPAYGEDDFYTCKRYGMSNDDILSPVQSNGVYVDSLPFFGGQFIWKANPNVVAKLEEVGSLLAHETINHSYMHCWRHKTPLIYRATAQWFVGMDKQPRQGASLRERALEAITQTEFVPGWGQARLHGMIAGRPDWCISRQRNWGVPIPFFLHKASGELHPRTVELMEEVAQRVEKEGIEAWFKLDAAELLGEEAAQYEKINDTLDVWFDSGTTHWHVLRGSHRIGHASGPVADLYLEGSDQHRGWFHSSLLTGCAIDNHAPYRQLLTHGFTVDESGRKMSKSLGNTVVPQTVIDTLGADILRLWVASTDYSGEIAVSQQILQRSADAYRRIRNTTRFLLSNLNGFDPATDLLPPQEMLALDRWAVDRALLLQREIEEAYREYRFWNVYSKVHNFCVQELGGFYLDIIKDRQYTTGANSVARRSCQTALFHIAEALVRWIAPILAFTAEEVWKFLPGERAESVMLATWYDGLNELPADVTLNRQYWEQVMAVKAAVNKELENQRAAKAVGGNLQAEVTLYAEDALQAQLAKLGNELRFVLITSTATLAPLSAAPADAVDSEVAGLKLKVVKSTHAKCGRCWHHREDVGQHAAHPDLCGRCIENIEGSGEVRHYA |
| References |
| External Links: |
|
| General Reference: |
PaperBLAST - Find papers about PA4560 and its homologs
|
