P. aeruginosa Metabolome Database Protein PA4280

Identification

Name:

Bifunctional protein BirA

Synonyms:

Biotin operon repressor
Biotin--[acetyl-CoA-carboxylase] synthetase
Biotin--protein ligase

Gene Name:

birA

Enzyme Class:

6.3.4.15

Biological Properties

General Function:

Involved in biotin-[acetyl-CoA-carboxylase] ligase activity

Specific Function:

BirA acts both as a biotin-operon repressor and as the enzyme that synthesizes the corepressor, acetyl-CoA:carbon-dioxide ligase. This protein also activates biotin to form biotinyl-5'- adenylate and transfers the biotin moiety to biotin-accepting proteins

Cellular Location:

Not Available

KEGG Pathways:

Biotin metabolism pae00780
Metabolic pathways pae01100

KEGG Reactions:

↔

Adenosine triphosphate + Biotin ↔ Pyrophosphate + Biotinyl-5'-AMP
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Apo-[carboxylase]

↔

Holo-[carboxylase]

Biotinyl-5'-AMP + Apo-[carboxylase] ↔ Adenosine monophosphate + Holo-[carboxylase]
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Apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)]

↔

[Acetyl-CoA:carbon-dioxide ligase (ADP-forming)]

Adenosine triphosphate + Biotin + Apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)] ↔ Adenosine monophosphate + Pyrophosphate + [Acetyl-CoA:carbon-dioxide ligase (ADP-forming)]
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SMPDB Reactions:

→

diphosphate

Biotin + Adenosine triphosphate → diphosphate + Biotinyl-5'-AMP + Pyrophosphate
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apo-[carboxylase]

→

Biotin-Carboxyl Carrying Protein

Biotinyl-5'-AMP + apo-[carboxylase] → Adenosine monophosphate + Biotin-Carboxyl Carrying Protein
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Biotin-Carboxyl Carrying Protein

→

Pyrophosphate

Biotinylated [BCCP monomer]

Adenosine triphosphate + Biotin + Biotin-Carboxyl Carrying Protein → Hydrogen ion + Adenosine monophosphate + Pyrophosphate + Biotinylated [BCCP monomer]
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PseudoCyc/BioCyc Reactions:

↔

Adenosine triphosphate + Biotin ↔ Pyrophosphate + Biotinyl-5'-AMP
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Apo-[carboxylase]

↔

Holo-[carboxylase]

Biotinyl-5'-AMP + Apo-[carboxylase] ↔ Adenosine monophosphate + Holo-[carboxylase]
ReactionCard

apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)]

→

[acetyl-CoA:carbon-dioxide ligase (ADP-forming)]

Adenosine triphosphate + Biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)] → Adenosine monophosphate + Pyrophosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)]
ReactionCard

Apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)]

↔

[Acetyl-CoA:carbon-dioxide ligase (ADP-forming)]

Adenosine triphosphate + Biotin + Apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)] ↔ Adenosine monophosphate + Pyrophosphate + [Acetyl-CoA:carbon-dioxide ligase (ADP-forming)]
ReactionCard

→

diphosphate

Biotin + Adenosine triphosphate → diphosphate + Biotinyl-5'-AMP + Pyrophosphate
ReactionCard

apo-[carboxylase]

→

Biotin-Carboxyl Carrying Protein

Biotinyl-5'-AMP + apo-[carboxylase] → Adenosine monophosphate + Biotin-Carboxyl Carrying Protein
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→

biotin + ATP → diphosphate + AMP
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Complex Reactions:

apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)]

→

[acetyl-CoA:carbon-dioxide ligase (ADP-forming)]

Adenosine triphosphate + Biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)] → Adenosine monophosphate + Pyrophosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)]
ReactionCard

Transports:

Not Available

Metabolites:

PAMDB ID	Name	View
PAMDB000014	Adenosine monophosphate	MetaboCard
PAMDB000148	Adenosine triphosphate	MetaboCard
PAMDB000008	Biotin	MetaboCard
PAMDB000655	Biotinyl-5'-AMP	MetaboCard
PAMDB001633	Hydrogen ion	MetaboCard
PAMDB000633	Pyrophosphate	MetaboCard

GO Classification:

Function
biotin-protein ligase activity
biotin-[acetyl-CoA-carboxylase] ligase activity
catalytic activity
ligase activity
ligase activity, forming carbon-nitrogen bonds
transcription regulator activity
transcription repressor activity
Process
biological regulation
macromolecule metabolic process
macromolecule modification
metabolic process
protein modification process
regulation of biological process
regulation of gene expression
regulation of macromolecule metabolic process
regulation of metabolic process
regulation of transcription
regulation of transcription, DNA-dependent

Gene Properties

Locus tag:

PA4280

Strand:

Entrez Gene ID:

881668

Accession:

NP_252970.1

GI:

15599476

Sequence start:

4787477

Sequence End:

4788415

Sequence Length:

938

Gene Sequence:

>PA4280
ATGCAGACCTTGCTGAAACTACTTCAAGACGGGCGATTCCACTCCGGTGAGGAGTTGGGGGCGGTACTGGGCATCAGTCGCAGTGCAGTCTGGAAACGCTTGCAGCACCTGGAGGCTGAACATGGCCTGCAGTTCCACAAGGTGCGTGGCCGGGGTTACCGCCTGGCGAGTCCCCTCAGTCTCCTGGATCCGCGGAAGATCGACAGCCTCTGGCCTGTCGAGATCATGCCCACCGTCGATTCGACCAATAGCGAGGCCATGCGCAGGCTCGCGGCTGGAGCCTCGACGCCTTTCGTTATCGTCGCCGAGCAGCAGACTGCCGGGCGTGGACGTCGCGGGCGAGCCTGGGTCAGTCCCTACGGAGAGAACCTCTATTATTCTCTGGCCATTACTGTCAGGGGGGGCGCTCGGGAGCTCGAAGGGTTGAGCCTGGTTGTCGGTCTCGCGGTGCTCCGGGCCCTGGAGTCTTTGGGCGTCCAGGACACCGGGTTGAAGTGGCCGAACGACCTTCTGCAGGGAGGCAAGAAGATCGCCGGAATTCTCCTGGAGCTGAGTGGCGACCCCGCGGATCTGTGTCATGTCGTTATCGGCATAGGGATCAACGTCAACATGAAGACCGCCACTGATATCGGTCAGCCTTGGACCTCCCTGCGCGAAGCGCTGGGGGAGCTGGTGGATCGTTCGCGGCTGCTTTCTGCTCTCAATCGGCAGCTGGCTGATTATCTCGATAGGCATTCGCGCGAAGGCTTTGCTGCAAGTCGCGAGGAGTGGGAGTCCTGCAATCTCTGGCAGGGTGCCCAGGTCATTCTCTCGACTGCCCTGAGTTCCATCGAGGGTCGTGTGCTGGGTGTCGATAGCCGAGGAGCGCTGCGTCTTGAGGTGGATGGCGTGGAGCAGGGGTTCAGTGGAGGGGAGCTTAGTTTGAGGTTGCGTCAATGA

Protein Properties

Protein Residues:

312

Protein Molecular Weight:

34 kDa

Protein Theoretical pI:

7.67

Hydropathicity (GRAVY score):

-0.125

Charge at pH 7 (predicted):

1.39

Protein Sequence:

>PA4280
MQTLLKLLQDGRFHSGEELGAVLGISRSAVWKRLQHLEAEHGLQFHKVRGRGYRLASPLSLLDPRKIDSLWPVEIMPTVDSTNSEAMRRLAAGASTPFVIVAEQQTAGRGRRGRAWVSPYGENLYYSLAITVRGGARELEGLSLVVGLAVLRALESLGVQDTGLKWPNDLLQGGKKIAGILLELSGDPADLCHVVIGIGINVNMKTATDIGQPWTSLREALGELVDRSRLLSALNRQLADYLDRHSREGFAASREEWESCNLWQGAQVILSTALSSIEGRVLGVDSRGALRLEVDGVEQGFSGGELSLRLRQ

References

External Links:

Resource	Link
Genome ID:	PA4280
Entrez Gene ID:	881668
NCBI Protein ID:	15599476

General Reference:

PaperBLAST - Find papers about PA4280 and its homologs

Bifunctional protein BirA (PA4280)