Identification
Name: Bifunctional protein BirA
Synonyms:
  • Biotin operon repressor
  • Biotin--[acetyl-CoA-carboxylase] synthetase
  • Biotin--protein ligase
Gene Name: birA
Enzyme Class:
Biological Properties
General Function: Involved in biotin-[acetyl-CoA-carboxylase] ligase activity
Specific Function: BirA acts both as a biotin-operon repressor and as the enzyme that synthesizes the corepressor, acetyl-CoA:carbon-dioxide ligase. This protein also activates biotin to form biotinyl-5'- adenylate and transfers the biotin moiety to biotin-accepting proteins
Cellular Location: Not Available
KEGG Pathways:
KEGG Reactions:
Thumb+ThumbThumb+Thumb
Thumb+Apo-[carboxylase]Thumb+Holo-[carboxylase]
Biotinyl-5'-AMP + Apo-[carboxylase] ↔ Adenosine monophosphate + Holo-[carboxylase]
ReactionCard
Thumb+Thumb+Apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)]Thumb+Thumb+[Acetyl-CoA:carbon-dioxide ligase (ADP-forming)]
Adenosine triphosphate + Biotin + Apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)] ↔ Adenosine monophosphate + Pyrophosphate + [Acetyl-CoA:carbon-dioxide ligase (ADP-forming)]
ReactionCard
SMPDB Reactions:
Thumb+Thumbdiphosphate+Thumb+Thumb
Thumb+apo-[carboxylase]Thumb+Biotin-Carboxyl Carrying Protein
Biotinyl-5'-AMP + apo-[carboxylase] → Adenosine monophosphate + Biotin-Carboxyl Carrying Protein
ReactionCard
Thumb+Thumb+Biotin-Carboxyl Carrying ProteinThumb+Thumb+Pyrophosphate+Biotinylated [BCCP monomer]
Adenosine triphosphate + Biotin + Biotin-Carboxyl Carrying Protein → Hydrogen ion + Adenosine monophosphate + Pyrophosphate + Biotinylated [BCCP monomer]
ReactionCard
PseudoCyc/BioCyc Reactions:
Thumb+ThumbThumb+Thumb
Thumb+Apo-[carboxylase]Thumb+Holo-[carboxylase]
Biotinyl-5'-AMP + Apo-[carboxylase] ↔ Adenosine monophosphate + Holo-[carboxylase]
ReactionCard
Thumb+Thumb+apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)]Thumb+Thumb+[acetyl-CoA:carbon-dioxide ligase (ADP-forming)]
Adenosine triphosphate + Biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)] → Adenosine monophosphate + Pyrophosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)]
ReactionCard
Thumb+Thumb+Apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)]Thumb+Thumb+[Acetyl-CoA:carbon-dioxide ligase (ADP-forming)]
Adenosine triphosphate + Biotin + Apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)] ↔ Adenosine monophosphate + Pyrophosphate + [Acetyl-CoA:carbon-dioxide ligase (ADP-forming)]
ReactionCard
Thumb+Thumbdiphosphate+Thumb+Thumb
Thumb+apo-[carboxylase]Thumb+Biotin-Carboxyl Carrying Protein
Biotinyl-5'-AMP + apo-[carboxylase] → Adenosine monophosphate + Biotin-Carboxyl Carrying Protein
ReactionCard
PAMDB110776+PAMDB110477PAMDB110246+PAMDB110478
Complex Reactions:
Thumb+Thumb+apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)]Thumb+Thumb+[acetyl-CoA:carbon-dioxide ligase (ADP-forming)]
Adenosine triphosphate + Biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)] → Adenosine monophosphate + Pyrophosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)]
ReactionCard
Transports: Not Available
Metabolites:
PAMDB IDNameView
PAMDB000014Adenosine monophosphateMetaboCard
PAMDB000148Adenosine triphosphateMetaboCard
PAMDB000008BiotinMetaboCard
PAMDB000655Biotinyl-5'-AMPMetaboCard
PAMDB001633Hydrogen ionMetaboCard
PAMDB000633PyrophosphateMetaboCard
GO Classification:
Function
biotin-protein ligase activity
biotin-[acetyl-CoA-carboxylase] ligase activity
catalytic activity
ligase activity
ligase activity, forming carbon-nitrogen bonds
transcription regulator activity
transcription repressor activity
Process
biological regulation
macromolecule metabolic process
macromolecule modification
metabolic process
protein modification process
regulation of biological process
regulation of gene expression
regulation of macromolecule metabolic process
regulation of metabolic process
regulation of transcription
regulation of transcription, DNA-dependent
Gene Properties
Locus tag: PA4280
Strand: -
Entrez Gene ID: 881668
Accession: NP_252970.1
GI: 15599476
Sequence start: 4787477
Sequence End: 4788415
Sequence Length: 938
Gene Sequence:
>PA4280
ATGCAGACCTTGCTGAAACTACTTCAAGACGGGCGATTCCACTCCGGTGAGGAGTTGGGGGCGGTACTGGGCATCAGTCGCAGTGCAGTCTGGAAACGCTTGCAGCACCTGGAGGCTGAACATGGCCTGCAGTTCCACAAGGTGCGTGGCCGGGGTTACCGCCTGGCGAGTCCCCTCAGTCTCCTGGATCCGCGGAAGATCGACAGCCTCTGGCCTGTCGAGATCATGCCCACCGTCGATTCGACCAATAGCGAGGCCATGCGCAGGCTCGCGGCTGGAGCCTCGACGCCTTTCGTTATCGTCGCCGAGCAGCAGACTGCCGGGCGTGGACGTCGCGGGCGAGCCTGGGTCAGTCCCTACGGAGAGAACCTCTATTATTCTCTGGCCATTACTGTCAGGGGGGGCGCTCGGGAGCTCGAAGGGTTGAGCCTGGTTGTCGGTCTCGCGGTGCTCCGGGCCCTGGAGTCTTTGGGCGTCCAGGACACCGGGTTGAAGTGGCCGAACGACCTTCTGCAGGGAGGCAAGAAGATCGCCGGAATTCTCCTGGAGCTGAGTGGCGACCCCGCGGATCTGTGTCATGTCGTTATCGGCATAGGGATCAACGTCAACATGAAGACCGCCACTGATATCGGTCAGCCTTGGACCTCCCTGCGCGAAGCGCTGGGGGAGCTGGTGGATCGTTCGCGGCTGCTTTCTGCTCTCAATCGGCAGCTGGCTGATTATCTCGATAGGCATTCGCGCGAAGGCTTTGCTGCAAGTCGCGAGGAGTGGGAGTCCTGCAATCTCTGGCAGGGTGCCCAGGTCATTCTCTCGACTGCCCTGAGTTCCATCGAGGGTCGTGTGCTGGGTGTCGATAGCCGAGGAGCGCTGCGTCTTGAGGTGGATGGCGTGGAGCAGGGGTTCAGTGGAGGGGAGCTTAGTTTGAGGTTGCGTCAATGA
Protein Properties
Protein Residues: 312
Protein Molecular Weight: 34 kDa
Protein Theoretical pI: 7.67
Hydropathicity (GRAVY score): -0.125
Charge at pH 7 (predicted): 1.39
Protein Sequence:
>PA4280
MQTLLKLLQDGRFHSGEELGAVLGISRSAVWKRLQHLEAEHGLQFHKVRGRGYRLASPLSLLDPRKIDSLWPVEIMPTVDSTNSEAMRRLAAGASTPFVIVAEQQTAGRGRRGRAWVSPYGENLYYSLAITVRGGARELEGLSLVVGLAVLRALESLGVQDTGLKWPNDLLQGGKKIAGILLELSGDPADLCHVVIGIGINVNMKTATDIGQPWTSLREALGELVDRSRLLSALNRQLADYLDRHSREGFAASREEWESCNLWQGAQVILSTALSSIEGRVLGVDSRGALRLEVDGVEQGFSGGELSLRLRQ
References
External Links:
Resource Link
Genome ID: PA4280
Entrez Gene ID: 881668
NCBI Protein ID: 15599476
General Reference: PaperBLAST - Find papers about PA4280 and its homologs