Thiamine-phosphate pyrophosphorylase (PA3976)

Identification
Name: Thiamine-phosphate pyrophosphorylase
Synonyms:
  • TMP pyrophosphorylase
  • TMP-PPase
  • Thiamine-phosphate synthase
Gene Name: thiE
Enzyme Class:
Biological Properties
General Function: Involved in catalytic activity
Specific Function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP)
Cellular Location: Not Available
KEGG Pathways:
KEGG Reactions:
Thumb+Thumb+Thumb ↔ Thumb+Thumb
2-Methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-Methyl-5-(2-phosphoethyl)-thiazole + Hydrogen ion ↔ Pyrophosphate + Thiamine monophosphate
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Thumb+Thumb ↔ Thumb+Thumb
2-Methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-Methyl-5-(2-phosphoethyl)-thiazole ↔ Pyrophosphate + Thiamine monophosphate
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SMPDB Reactions:
4-amino-2-methyl-5-diphosphomethylpyrimidine+Thumb+2 Thumb+Thumb → Thiamine monophosphate+Thumb+diphosphate+Thumb+Thumb
4-amino-2-methyl-5-diphosphomethylpyrimidine + 2-((2R,5Z)-2-Carboxy-4-methylthiazol-5(2H)-ylidene)ethyl phosphate + 2 Hydrogen ion + 2-Methyl-4-amino-5-hydroxymethylpyrimidine diphosphate → Thiamine monophosphate + Carbon dioxide + diphosphate + Thiamine monophosphate + Pyrophosphate
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Thumb+Thumb+Thumb → Thumb+Pyrophosphate+Thumb
2-Methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 2-((2R,5Z)-2-Carboxy-4-methylthiazol-5(2H)-ylidene)ethyl phosphate + Hydrogen ion → Carbon dioxide + Pyrophosphate + Thiamine monophosphate
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PseudoCyc/BioCyc Reactions:
Thumb+Thumb+Thumb ↔ Thumb+Thumb
2-Methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-Methyl-5-(2-phosphoethyl)-thiazole + Hydrogen ion ↔ Pyrophosphate + Thiamine monophosphate
ReactionCard
2-[(2R,5Z)-(2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate+Thumb+Thumb → Thumb+Thumb+Thumb
2-[(2R,5Z)-(2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate + 2-Methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + Hydrogen ion → Thiamine monophosphate + Carbon dioxide + Pyrophosphate
ReactionCard
Thumb+Thumb+Thumb → Thumb+Thumb
Hydrogen ion + 4-Methyl-5-(2-phosphoethyl)-thiazole + 2-Methyl-4-amino-5-hydroxymethylpyrimidine diphosphate → Thiamine monophosphate + Pyrophosphate
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Complex Reactions:
Thumb+Thumb → Thumb+Thumb
2-Methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-Methyl-5-(2-phosphoethyl)-thiazole → Pyrophosphate + Thiamine monophosphate
ReactionCard
Transports: Not Available
Metabolites:
PAMDB IDNameView
PAMDB0034432-((2R,5Z)-2-Carboxy-4-methylthiazol-5(2H)-ylidene)ethyl phosphateMetaboCard
PAMDB0008952-Methyl-4-amino-5-hydroxymethylpyrimidine diphosphateMetaboCard
PAMDB0009424-Methyl-5-(2-phosphoethyl)-thiazoleMetaboCard
PAMDB000568Carbon dioxideMetaboCard
PAMDB001633Hydrogen ionMetaboCard
PAMDB000633PyrophosphateMetaboCard
PAMDB000462Thiamine monophosphateMetaboCard
GO Classification:
Function
catalytic activity
thiamin-phosphate diphosphorylase activity
transferase activity
transferase activity, transferring alkyl or aryl (other than methyl) groups
Process
cellular aromatic compound metabolic process
cellular metabolic process
metabolic process
thiamin and derivative metabolic process
thiamin biosynthetic process
thiamin metabolic process
Gene Properties
Locus tag: PA3976
Strand: +
Entrez Gene ID: 880858
Accession: NP_252665.1
GI: 15599171
Sequence start: 4456695
Sequence End: 4457324
Sequence Length: 629
Gene Sequence: 
>PA3976
ATGAAACTGCGCGGCCTGTACGCCATCACCGATAGCCAGCTACTGGACGACGGCCGCCTGCTGCCCTACGTCGAAGCCGCGCTCCGCGGCGGCGCGCGCCTGCTGCAATACCGCGACAAATCCAGCGACCAGGCGCGGCGCCTGCGCGAGGCGGAAAGCCTGCGCGAACTCTGCGAACGCCACGGCGCGCAGTTGATCGTCAACGACGACGCCGAACTCGCCGCGCGCCTGGGCGTCGGCCTGCACCTCGGCCAGACCGACGGTTCGCTGTCGGCGGCGCGCGCCCTGCTCGGCCGCCAGGCGATCATCGGCGCCACCTGCCACGCCCAACTGGAACTGGCCGAGCAGGCGGTGGCCGAGGGCGCCAGCTACGTCGCCTTCGGCCGCTTCTTCAATTCCAGCACCAAACCCGGCGCCCCCGCCGCCAGCGTCGAACTGCTCGACCAGGCCCGGCCGCGCCTGCACCTGCCGATCACCGCCATCGGCGGCATCAGCCTCGACACCGCGCCCGGCCTGATCGCCCGCGGCGTCGATCTGGTCGCCGTGATCCATGCGCTGTTCGCCGCCGCGTCCGCCGCCGAGGTGGAACGCCGCGCCCGCGCCTTCAGCGCCCTGTTCGAACCTGCCTGA
Protein Properties
Protein Residues: 209
Protein Molecular Weight: 22.1 kDa
Protein Theoretical pI: 7.06
Hydropathicity (GRAVY score): 0.076
Charge at pH 7 (predicted): 0.14
Protein Sequence: 
>PA3976
MKLRGLYAITDSQLLDDGRLLPYVEAALRGGARLLQYRDKSSDQARRLREAESLRELCERHGAQLIVNDDAELAARLGVGLHLGQTDGSLSAARALLGRQAIIGATCHAQLELAEQAVAEGASYVAFGRFFNSSTKPGAPAASVELLDQARPRLHLPITAIGGISLDTAPGLIARGVDLVAVIHALFAAASAAEVERRARAFSALFEPA
References
External Links:
Resource Link
Genome ID: PA3976
Entrez Gene ID: 880858
NCBI Protein ID: 15599171
General Reference: PaperBLAST - Find papers about PA3976 and its homologs