Menaquinol 8 (PAMDB001653)

General function:

Involved in oxidation-reduction process

Specific function:

Transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Does not couple the redox reaction to proton translocation

Gene Name:

ndh

Locus Tag:

PA4538

Molecular weight:

47.4 kDa

Reactions

General function:

Involved in oxidoreductase activity

Specific function:

The nitrate reductase enzyme complex allows Pseudomonas aeruginosa to use nitrate as an electron acceptor during anaerobic growth. The alpha chain is the actual site of nitrate reduction

Gene Name:

narG

Locus Tag:

PA3875

Molecular weight:

141 kDa

Reactions

General function:

Involved in catalytic activity

Specific function:

(S)-dihydroorotate + a quinone = orotate + a quinol

Gene Name:

pyrD

Locus Tag:

PA3050

Molecular weight:

36.1 kDa

Reactions

General function:

Involved in electron carrier activity

Specific function:

Formate dehydrogenase allows Pseudomonas aeruginosa to use formate as major electron donor during anaerobic respiration, when nitrate is used as electron acceptor. The beta chain is an electron transfer unit containing 4 cysteine clusters involved in the formation of iron-sulfur centers. Electrons are transferred from the gamma chain to the molybdenum cofactor of the alpha subunit

Gene Name:

fdnH

Locus Tag:

PA4811

Molecular weight:

33.8 kDa

General function:

Involved in respiratory electron transport chain

Specific function:

Formate dehydrogenase allows Pseudomonas aeruginosa to use formate as major electron donor during anaerobic respiration, when nitrate is used as electron acceptor. Subunit gamma is the cytochrome b556(FDN) component of the formate dehydrogenase

Gene Name:

fdnI

Locus Tag:

PA4810

Molecular weight:

23.9 kDa

General function:

Involved in catalytic activity

Specific function:

Specific function unknown

Gene Name:

glcD

Locus Tag:

PA5355

Molecular weight:

53.7 kDa

General function:

Involved in oxidoreductase activity, acting on NADH or NADPH

Specific function:

NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient

Gene Name:

nuoA

Locus Tag:

PA2637

Molecular weight:

15 kDa

Reactions

General function:

Involved in NADH dehydrogenase (ubiquinone) activity

Specific function:

NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient

Gene Name:

nuoB

Locus Tag:

PA2638

Molecular weight:

25.4 kDa

Reactions

General function:

Involved in oxidoreductase activity

Specific function:

NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient

Gene Name:

nuoE

Locus Tag:

PA2640

Molecular weight:

18.1 kDa

Reactions

General function:

Involved in oxidation-reduction process

Specific function:

NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient. This subunit may bind ubiquinone

Gene Name:

nuoH

Locus Tag:

PA2643

Molecular weight:

36.7 kDa

Reactions

General function:

Involved in electron carrier activity

Specific function:

NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient

Gene Name:

nuoI

Locus Tag:

PA2644

Molecular weight:

20.6 kDa

Reactions

General function:

Involved in NADH dehydrogenase (ubiquinone) activity

Specific function:

NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient

Gene Name:

nuoJ

Locus Tag:

PA2645

Molecular weight:

17.6 kDa

Reactions

General function:

Involved in oxidoreductase activity, acting on NADH or NADPH

Specific function:

There are 2 NADH dehydrogenases in Pseudomonas aeruginosa, however only this complex is able to use dNADH (reduced nicotinamide hypoxanthine dinucleotide, deamino-NADH) and dNADH-DB (dimethoxy- 5-methyl-6-decyl-1,4-benzoquinone) as substrates

Gene Name:

nuoK

Locus Tag:

PA2646

Molecular weight:

11 kDa

Reactions

General function:

Involved in NADH dehydrogenase (ubiquinone) activity

Specific function:

NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient

Gene Name:

nuoM

Locus Tag:

PA2648

Molecular weight:

55.7 kDa

Reactions

General function:

Involved in NADH dehydrogenase (ubiquinone) activity

Specific function:

NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient

Gene Name:

nuoN

Locus Tag:

PA2649

Molecular weight:

51.7 kDa

Reactions

General function:

Involved in electron carrier activity

Specific function:

Catalyzes the oxidation of L-aspartate to iminoaspartate

Gene Name:

nadB

Locus Tag:

PA0761

Molecular weight:

60 kDa

Reactions

General function:

Involved in iron-sulfur cluster binding

Specific function:

The nitrate reductase enzyme complex allows Pseudomonas aeruginosa to use nitrate as an electron acceptor during anaerobic growth. The beta chain is an electron transfer unit containing four cysteine clusters involved in the formation of iron-sulfur centers. Electrons are transferred from the gamma chain to the molybdenum cofactor of the alpha subunit

Gene Name:

narH

Locus Tag:

PA3874

Molecular weight:

58.1 kDa

Reactions

General function:

Involved in nitrate reductase activity

Specific function:

The nitrate reductase enzyme complex allows Pseudomonas aeruginosa to use nitrate as an electron acceptor during anaerobic growth. The gamma chain is a membrane-embedded heme-iron unit resembling cytochrome b, which transfers electrons from quinones to the beta subunit

Gene Name:

narI

Locus Tag:

PA3872

Molecular weight:

25 kDa

Reactions

General function:

Involved in formate dehydrogenase (NAD+) activity

Specific function:

Formate dehydrogenase allows Pseudomonas aeruginosa to use formate as major electron donor during anaerobic respiration, when nitrate is used as electron acceptor. The alpha subunit forms the active site

Gene Name:

fdnG

Locus Tag:

PA4812

Molecular weight:

104.7 kDa

Reactions

General function:

Involved in NADH dehydrogenase (ubiquinone) activity

Specific function:

NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient

Gene Name:

nuoF

Locus Tag:

PA2641

Molecular weight:

48.7 kDa

Reactions

General function:

Involved in catalytic activity

Specific function:

(S)-lactate + 2 ferricytochrome c = pyruvate + 2 ferrocytochrome c + 2 H(+)

Gene Name:

lldD

Locus Tag:

PA4771

Molecular weight:

41.1 kDa

Reactions

General function:

Involved in oxidoreductase activity, acting on NADH or NADPH

Specific function:

NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient

Gene Name:

nuoC

Locus Tag:

PA2639

Molecular weight:

68.3 kDa

Reactions

General function:

Involved in electron carrier activity

Specific function:

NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient

Gene Name:

nuoG

Locus Tag:

PA2642

Molecular weight:

99 kDa

Reactions

General function:

Involved in NADH dehydrogenase (ubiquinone) activity

Specific function:

NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient

Gene Name:

nuoL

Locus Tag:

PA2647

Molecular weight:

66.2 kDa

Reactions

General function:

Involved in oxidoreductase activity

Specific function:

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein napC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism

Gene Name:

napA

Locus Tag:

PA1174

Molecular weight:

92.9 kDa

Reactions

General function:

Involved in malate dehydrogenase (quinone) activity

Specific function:

(S)-malate + a quinone = oxaloacetate + reduced quinone

Gene Name:

mqo

Locus Tag:

PA3452

Molecular weight:

57.2 kDa

Reactions

General function:

Involved in iron-sulfur cluster binding

Specific function:

Specific function unknown

Gene Name:

glcF

Locus Tag:

PA5353

Molecular weight:

44.7 kDa

General function:

Involved in coenzyme binding

Specific function:

Specific function unknown

Gene Name:

mdaB

Locus Tag:

PA2580

Molecular weight:

22 kDa

General function:

Involved in catalytic activity

Specific function:

Specific function unknown

Gene Name:

glcE

Locus Tag:

PA5354

Molecular weight:

38.2 kDa

General function:

Involved in protein disulfide oxidoreductase activity

Specific function:

Required for disulfide bond formation in some periplasmic proteins such as phoA or ompA. Acts by transferring its disulfide bond to other proteins and is reduced in the process. DsbA is reoxidized by dsbB. It is required for pilus biogenesis

Gene Name:

dsbA

Locus Tag:

PA5489

Molecular weight:

23.4 kDa

General function:

Involved in methyltransferase activity

Specific function:

Methyltransferase required for the conversion of dimethylmenaquinone (DMKH2) to menaquinone (MKH2) and the conversion of 2-polyprenyl-6-methoxy-1,4-benzoquinol (DDMQH2) to 2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinol (DMQH2)

Gene Name:

ubiE

Locus Tag:

PA5063

Molecular weight:

28.3 kDa

Reactions

General function:

Energy production and conversion

Specific function:

Small subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme napC protein, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism

Gene Name:

napB

Locus Tag:

PA1173

Molecular weight:

17.9 kDa

General function:

Involved in protein disulfide oxidoreductase activity

Specific function:

Required for disulfide bond formation in some periplasmic proteins such as phoA or ompA. Acts by oxidizing the dsbA protein

Gene Name:

dsbB

Locus Tag:

PA0538

Molecular weight:

18.1 kDa

General function:

Involved in heme binding

Specific function:

Mediates electron flow from quinones to the napAB complex

Gene Name:

napC

Locus Tag:

PA1172

Molecular weight:

22.7 kDa

General function:

Involved in unfolded protein binding

Specific function:

Chaperone required for proper molybdenum cofactor insertion and final assembly of the membrane-bound respiratory nitrate reductase 1. Required for the insertion of the molybdenum into the apo-NarG subunit, maybe by keeping NarG in an appropriate competent-open conformation for the molybdenum cofactor insertion to occur. NarJ maintains the apoNarGH complex in a soluble state. Upon insertion of the molybdenum cofactor, NarJ seems to dissociate from the activated soluble NarGH complex, before its association with the NarI subunit on the membrane

Gene Name:

narJ

Locus Tag:

PA3873

Molecular weight:

27.3 kDa