NADH-quinone oxidoreductase subunit J (PA2645)

Identification
Name: NADH-quinone oxidoreductase subunit J
Synonyms:
  • NADH dehydrogenase I subunit J
  • NDH-1 subunit J
  • NUO10
Gene Name: nuoJ
Enzyme Class:
Biological Properties
General Function: Involved in NADH dehydrogenase (ubiquinone) activity
Specific Function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient
Cellular Location: Cell inner membrane; Multi-pass membrane protein
KEGG Pathways:
KEGG Reactions:
Thumb+Acceptor ↔ Thumb+Reduced acceptor
Ubiquinol-8 + Acceptor ↔ Ubiquinone-1 + Reduced acceptor
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Thumb+Thumb ↔ Thumb+Thumb
NADH + Ubiquinone-1 ↔ NAD + Ubiquinol-8
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SMPDB Reactions:
Thumb+5 Thumb+Thumb → Thumb+Thumb+Thumb
NADH + 5 Hydrogen ion + Ubiquinone-1 → Hydrogen ion + NAD + Ubiquinol-1
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Thumb+4 Thumb+2 Thumb+menaquinone-8 ? Thumb+Thumb+Thumb+Electron+4 Thumb
NADH + 4 Hydrogen ion + 2 Hydrogen ion + menaquinone-8 ? NAD + Hydrogen ion + Menaquinol 8 + Electron + 4 Hydrogen ion
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"a menaquinone"+4 Thumb+Thumb → "a menaquinol"+Thumb
"a menaquinone" + 4 Hydrogen ion + NADH → "a menaquinol" + NAD
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4 Thumb+Thumb+"a menaquinone" → Thumb+"a menaquinol"
4 Hydrogen ion + NADH + "a menaquinone" → NAD + "a menaquinol"
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PseudoCyc/BioCyc Reactions:
Thumb+Acceptor ↔ Thumb+Reduced acceptor
Ubiquinol-8 + Acceptor ↔ Ubiquinone-1 + Reduced acceptor
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Thumb+Thumb → Thumb+Thumb
NADH + Quinone → NAD + Hydroquinone
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Thumb+Thumb → Thumb+Thumb
NADH + Quinone → NAD + Hydroquinone
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Thumb+Thumb → Thumb+Thumb
NADH + Quinone → NAD + Hydroquinone
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Thumb+Thumb → Thumb+Thumb
NADH + Quinone → NAD + Hydroquinone
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Thumb+Thumb → Thumb+Thumb
NADH + Quinone → NAD + Hydroquinone
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Thumb+Thumb ↔ Thumb+Thumb
NADH + Ubiquinone-1 ↔ NAD + Ubiquinol-8
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Thumb+5 Thumb+Thumb → Thumb+Thumb+Thumb
NADH + 5 Hydrogen ion + Ubiquinone-1 → Hydrogen ion + NAD + Ubiquinol-1
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Thumb+4 Thumb+2 Thumb+menaquinone-8 ? Thumb+Thumb+Thumb+Electron+4 Thumb
NADH + 4 Hydrogen ion + 2 Hydrogen ion + menaquinone-8 ? NAD + Hydrogen ion + Menaquinol 8 + Electron + 4 Hydrogen ion
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Thumb
NADH + an oxidized unknown electron acceptor + H+ = NAD+ + an reduced unknown electron acceptor
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PAMDB110540+PAMDB001633+ETR-Quinones → PAMDB110542+ETR-Quinols
NADH + Hydrogen ion + ETR-Quinones → NAD+ + ETR-Quinols
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PAMDB000610+PAMDB110540+PAMDB001633 → PAMDB120506+PAMDB110542
Menaquinone + NADH + Hydrogen ion → menadiol + NAD+
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Complex Reactions:
Thumb+4 Thumb+Thumb → Thumb+Thumb+3 Thumb
2-Demethylmenaquinone 8 + 4 Hydrogen ion + NADH → 2-Demethylmenaquinol 8 + NAD + 3 Hydrogen ion
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4 Thumb+Thumb+Thumb → Thumb+Thumb+3 Thumb
4 Hydrogen ion + NADH + Ubiquinone-8 → NAD + Ubiquinol-8 + 3 Hydrogen ion
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4 Thumb+Menaquinone 8+Thumb → Thumb+Thumb+3 Thumb
4 Hydrogen ion + Menaquinone 8 + NADH → Menaquinol 8 + NAD + 3 Hydrogen ion
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Thumb+Thumb → Thumb+Thumb
NADH + Quinone → NAD + Hydroquinone
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Thumb+Thumb → Thumb+Thumb
NADH + Quinone → NAD + Hydroquinone
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Thumb+Thumb → Thumb+Thumb
NADH + Quinone → NAD + Hydroquinone
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Thumb+Thumb → Thumb+Thumb
NADH + Quinone → NAD + Hydroquinone
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Thumb+Thumb → Thumb+Thumb
NADH + Quinone → NAD + Hydroquinone
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Transports: Not Available
Metabolites:
PAMDB IDNameView
PAMDB0015632-Demethylmenaquinol 8MetaboCard
PAMDB0015642-Demethylmenaquinone 8MetaboCard
PAMDB001633Hydrogen ionMetaboCard
PAMDB000459HydroquinoneMetaboCard
PAMDB001653Menaquinol 8MetaboCard
PAMDB000197NADMetaboCard
PAMDB000398NADHMetaboCard
PAMDB003450QuinoneMetaboCard
PAMDB001968Ubiquinol-1MetaboCard
PAMDB000237Ubiquinol-8MetaboCard
PAMDB001833Ubiquinone-1MetaboCard
PAMDB001700Ubiquinone-8MetaboCard
GO Classification:
Function
catalytic activity
NADH dehydrogenase (quinone) activity
NADH dehydrogenase (ubiquinone) activity
NADH dehydrogenase activity
oxidoreductase activity
oxidoreductase activity, acting on NADH or NADPH
Process
metabolic process
oxidation reduction
Gene Properties
Locus tag: PA2645
Strand: +
Entrez Gene ID: 882354
Accession: NP_251335.1
GI: 15597841
Sequence start: 2992002
Sequence End: 2992502
Sequence Length: 500
Gene Sequence: 
>PA2645
GTGGAATTCGCTTTCTATTTCGCTGCCGGCGTCGCCGTACTGGCCACGCTGCGGGTCATCACCAACAGCAACCCGGTGCATGCCCTGCTCTACCTGATCATTTCCCTGCTGGCCATCTCGATGACCTTCTTCAGCCTCGGCGCACCCTTCGCCGGTGCCCTGGAGATCATCGTCTACGCTGGCGCCATCATGGTTCTGTTCGTCTTCGTGGTGATGATGCTCAACCTCGGCCCGGCCGTGGTCGAGCAGGAGCGCAAGTGGCTCACGCCCGGCATCTGGGTCGGGCCTTCGGCCCTGGCCCTGGTCCTGCTGGTGGAACTGCTGGTGGTCCTCGCCCGCACCCCGAGCGGTGCCGGCATCGGCCATACCACGGTAGATGCCAAGGCGGTCGGCATCAGCCTCTACGGCCCGTACCTGCTGGTGGTCGAACTGGCCTCCATGCTGTTGCTCGCGGCCCTGGTCGCTGCCTATCACCTGGGTCGTCAAGACGCCAAACAATAA
Protein Properties
Protein Residues: 166
Protein Molecular Weight: 17.6 kDa
Protein Theoretical pI: 6.8
Hydropathicity (GRAVY score): 1.198
Charge at pH 7 (predicted): -0.3
Protein Sequence: 
>PA2645
MEFAFYFAAGVAVLATLRVITNSNPVHALLYLIISLLAISMTFFSLGAPFAGALEIIVYAGAIMVLFVFVVMMLNLGPAVVEQERKWLTPGIWVGPSALALVLLVELLVVLARTPSGAGIGHTTVDAKAVGISLYGPYLLVVELASMLLLAALVAAYHLGRQDAKQ
References
External Links:
Resource Link
Genome ID: PA2645
Entrez Gene ID: 882354
NCBI Protein ID: 15597841
General Reference: PaperBLAST - Find papers about PA2645 and its homologs