NADH-quinone oxidoreductase subunit N (PA2649)

Identification
Name: NADH-quinone oxidoreductase subunit N
Synonyms:
  • NADH dehydrogenase I subunit N
  • NDH-1 subunit N
  • NUO14
Gene Name: nuoN
Enzyme Class:
Biological Properties
General Function: Involved in NADH dehydrogenase (ubiquinone) activity
Specific Function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient
Cellular Location: Cell inner membrane; Multi-pass membrane protein
KEGG Pathways:
KEGG Reactions:
Thumb+Acceptor ↔ Thumb+Reduced acceptor
Ubiquinol-8 + Acceptor ↔ Ubiquinone-1 + Reduced acceptor
ReactionCard
Thumb+Thumb ↔ Thumb+Thumb
NADH + Ubiquinone-1 ↔ NAD + Ubiquinol-8
ReactionCard
SMPDB Reactions:
Thumb+5 Thumb+Thumb → Thumb+Thumb+Thumb
NADH + 5 Hydrogen ion + Ubiquinone-1 → Hydrogen ion + NAD + Ubiquinol-1
ReactionCard
Thumb+4 Thumb+2 Thumb+menaquinone-8 ? Thumb+Thumb+Thumb+Electron+4 Thumb
NADH + 4 Hydrogen ion + 2 Hydrogen ion + menaquinone-8 ? NAD + Hydrogen ion + Menaquinol 8 + Electron + 4 Hydrogen ion
ReactionCard
"a menaquinone"+4 Thumb+Thumb → "a menaquinol"+Thumb
"a menaquinone" + 4 Hydrogen ion + NADH → "a menaquinol" + NAD
ReactionCard
4 Thumb+Thumb+"a menaquinone" → Thumb+"a menaquinol"
4 Hydrogen ion + NADH + "a menaquinone" → NAD + "a menaquinol"
ReactionCard
PseudoCyc/BioCyc Reactions:
Thumb+Acceptor ↔ Thumb+Reduced acceptor
Ubiquinol-8 + Acceptor ↔ Ubiquinone-1 + Reduced acceptor
ReactionCard
Thumb+Thumb → Thumb+Thumb
NADH + Quinone → NAD + Hydroquinone
ReactionCard
Thumb+Thumb ↔ Thumb+Thumb
NADH + Ubiquinone-1 ↔ NAD + Ubiquinol-8
ReactionCard
Thumb+5 Thumb+Thumb → Thumb+Thumb+Thumb
NADH + 5 Hydrogen ion + Ubiquinone-1 → Hydrogen ion + NAD + Ubiquinol-1
ReactionCard
Thumb+4 Thumb+2 Thumb+menaquinone-8 ? Thumb+Thumb+Thumb+Electron+4 Thumb
NADH + 4 Hydrogen ion + 2 Hydrogen ion + menaquinone-8 ? NAD + Hydrogen ion + Menaquinol 8 + Electron + 4 Hydrogen ion
ReactionCard
Thumb
NADH + an oxidized unknown electron acceptor + H+ = NAD+ + an reduced unknown electron acceptor
ReactionCard
PAMDB110540+PAMDB001633+ETR-Quinones → PAMDB110542+ETR-Quinols
NADH + Hydrogen ion + ETR-Quinones → NAD+ + ETR-Quinols
ReactionCard
PAMDB000610+PAMDB110540+PAMDB001633 → PAMDB120506+PAMDB110542
Menaquinone + NADH + Hydrogen ion → menadiol + NAD+
ReactionCard
Complex Reactions:
Thumb+4 Thumb+Thumb → Thumb+Thumb+3 Thumb
2-Demethylmenaquinone 8 + 4 Hydrogen ion + NADH → 2-Demethylmenaquinol 8 + NAD + 3 Hydrogen ion
ReactionCard
4 Thumb+Thumb+Thumb → Thumb+Thumb+3 Thumb
4 Hydrogen ion + NADH + Ubiquinone-8 → NAD + Ubiquinol-8 + 3 Hydrogen ion
ReactionCard
4 Thumb+Menaquinone 8+Thumb → Thumb+Thumb+3 Thumb
4 Hydrogen ion + Menaquinone 8 + NADH → Menaquinol 8 + NAD + 3 Hydrogen ion
ReactionCard
Thumb+Thumb → Thumb+Thumb
NADH + Quinone → NAD + Hydroquinone
ReactionCard
Transports: Not Available
Metabolites:
PAMDB IDNameView
PAMDB0015632-Demethylmenaquinol 8MetaboCard
PAMDB0015642-Demethylmenaquinone 8MetaboCard
PAMDB001633Hydrogen ionMetaboCard
PAMDB000459HydroquinoneMetaboCard
PAMDB001653Menaquinol 8MetaboCard
PAMDB000197NADMetaboCard
PAMDB000398NADHMetaboCard
PAMDB003450QuinoneMetaboCard
PAMDB001968Ubiquinol-1MetaboCard
PAMDB000237Ubiquinol-8MetaboCard
PAMDB001833Ubiquinone-1MetaboCard
PAMDB001700Ubiquinone-8MetaboCard
GO Classification:
Function
catalytic activity
NADH dehydrogenase (quinone) activity
NADH dehydrogenase (ubiquinone) activity
NADH dehydrogenase activity
oxidoreductase activity
oxidoreductase activity, acting on NADH or NADPH
Process
ATP synthesis coupled electron transport
cellular metabolic process
electron transport chain
generation of precursor metabolites and energy
metabolic process
oxidation reduction
respiratory electron transport chain
Gene Properties
Locus tag: PA2649
Strand: +
Entrez Gene ID: 882358
Accession: NP_251339.1
GI: 15597845
Sequence start: 2996265
Sequence End: 2997725
Sequence Length: 1460
Gene Sequence: 
>PA2649
ATGACCTTTACGATCCAACACTTCATCGCGCTCCTGCCACTGCTGATCACCAGCGCCACCCTGGTGGTGGTGATGCTGGCGGTAGCCTGGAAACGCAATCACTCGTTCACCGCCACCCTCTCGGTGATCGGCCTCAACCTGGCCCTGCTGTCGCTGCTGCCGGTCCTCGGCGTCACCCCGATCGAGGTGACACCGCTGGTGCTGGTGGACAACTACGCCTGCTTCTACATGGCCCTGGTGCTGGTCTCCGCCCTGGCCTGCGTGACCCTCGCCCACGCCTACATGGAAAGCTATCCGGGTAACCGCGAAGAGCTGTACCTGCTCCTGCTGCTGGCCACCGCCGGCGGCCTGGTGCTGGTCAGCGCGCAGCACCTGGCCAGCCTGTTCATCGGCCTGGAGCTGCTCTCGGTCCCGGTCTACGGGATGGTCGCCTACGCCTTCTTCAACAAGCGCTCGCTGGAAGCCGGCATCAAGTACACCGTGCTCTCGGCCGCCGGCTCCGCCTTCCTGCTGTTCGGCATGGCCCTGCTCTACGCCGAGTCCGGTACCCTGGGCTTCGCCGGCCTTGGCGCCAAGGTCGCCGAGCACGTGCTGAGCGGCCCGCTGGTCAGCGTTGGCGTAGGCATGATGCTGGTCGGCCTGGGCTTCAAGCTGTCGCTGGTGCCCTTCCACCTGTGGACCCCGGATGTCTACGAAGGCGCCCCGGCGCCGGTCTCGGCGTTCCTCGCCACCGCCAGCAAGGTCGCGGTGTTCGCCGTGCTGCTGCGGCTGTTCCAGATCGCCCCGGCGGCCCTGGACAACCAACTGCTGAACATCTCCCTGAGCGTCATCGCGGTCGCCTCGATCCTCTTCGGCAACCTGCTGGCACTGACCCAGAGCAACATCAAGCGCCTGCTCGGCTACTCGTCCATCGCCCACCTGGGCTACCTGCTGGTCGCCCTGATCGCCAGCAAGGGCATGGCCGTGGAAGCGGTCGGCGTCTACCTGGCTACCTACGTACTGACCTCCCTCGGCGCGTTCGGCGTGATCACCCTGATGTCCACCCCGTACAGCGGCCGCGACGCCGATGCGCTGTTCGAGTACCGCGGCTTGTTCTGGCGCCGGCCGGTACTGACCGCGGTGATGACCGTGATGATGCTGTCGCTGGCAGGCATCCCGCTGACCGCCGGCTTCATCGGCAAGTTCTACGTGATCGCGGTCGGCGTCGAGTCGCACCTGTGGTGGCTGATCGGCGCCCTGGTGCTGGGCAGCGCCATCGGCCTGTACTACTACCTGCGGGTCATGGTCACCCTGTTCCTGGTCGAGCCCGGCATCCGCCAGCACGACGCACCGTTCAACTGGGGCCAGCGCGCCGGCGGCATCATGCTGGTCGCCATCGCCCTGCTGGCGTTCTTCCTCGGCGTCTACCCGCAGCCGCTGCTGGAAATCCTCCAGCATTCCGGCCTGGCCCTCGCCGGCTGA
Protein Properties
Protein Residues: 486
Protein Molecular Weight: 51.7 kDa
Protein Theoretical pI: 8.18
Hydropathicity (GRAVY score): 1.052
Charge at pH 7 (predicted): 3.32
Protein Sequence: 
>PA2649
MTFTIQHFIALLPLLITSATLVVVMLAVAWKRNHSFTATLSVIGLNLALLSLLPVLGVTPIEVTPLVLVDNYACFYMALVLVSALACVTLAHAYMESYPGNREELYLLLLLATAGGLVLVSAQHLASLFIGLELLSVPVYGMVAYAFFNKRSLEAGIKYTVLSAAGSAFLLFGMALLYAESGTLGFAGLGAKVAEHVLSGPLVSVGVGMMLVGLGFKLSLVPFHLWTPDVYEGAPAPVSAFLATASKVAVFAVLLRLFQIAPAALDNQLLNISLSVIAVASILFGNLLALTQSNIKRLLGYSSIAHLGYLLVALIASKGMAVEAVGVYLATYVLTSLGAFGVITLMSTPYSGRDADALFEYRGLFWRRPVLTAVMTVMMLSLAGIPLTAGFIGKFYVIAVGVESHLWWLIGALVLGSAIGLYYYLRVMVTLFLVEPGIRQHDAPFNWGQRAGGIMLVAIALLAFFLGVYPQPLLEILQHSGLALAG
References
External Links:
Resource Link
Genome ID: PA2649
Entrez Gene ID: 882358
NCBI Protein ID: 15597845
General Reference: PaperBLAST - Find papers about PA2649 and its homologs