NADH-quinone oxidoreductase subunit M (PA2648)

Identification
Name: NADH-quinone oxidoreductase subunit M
Synonyms:
  • NADH dehydrogenase I subunit M
  • NDH-1 subunit M
  • NUO13
Gene Name: nuoM
Enzyme Class:
Biological Properties
General Function: Involved in NADH dehydrogenase (ubiquinone) activity
Specific Function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient
Cellular Location: Cell inner membrane; Multi-pass membrane protein
KEGG Pathways:
KEGG Reactions:
Thumb+Acceptor ↔ Thumb+Reduced acceptor
Ubiquinol-8 + Acceptor ↔ Ubiquinone-1 + Reduced acceptor
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Thumb+Thumb ↔ Thumb+Thumb
NADH + Ubiquinone-1 ↔ NAD + Ubiquinol-8
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SMPDB Reactions:
Thumb+5 Thumb+Thumb → Thumb+Thumb+Thumb
NADH + 5 Hydrogen ion + Ubiquinone-1 → Hydrogen ion + NAD + Ubiquinol-1
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Thumb+4 Thumb+2 Thumb+menaquinone-8 ? Thumb+Thumb+Thumb+Electron+4 Thumb
NADH + 4 Hydrogen ion + 2 Hydrogen ion + menaquinone-8 ? NAD + Hydrogen ion + Menaquinol 8 + Electron + 4 Hydrogen ion
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"a menaquinone"+4 Thumb+Thumb → "a menaquinol"+Thumb
"a menaquinone" + 4 Hydrogen ion + NADH → "a menaquinol" + NAD
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4 Thumb+Thumb+"a menaquinone" → Thumb+"a menaquinol"
4 Hydrogen ion + NADH + "a menaquinone" → NAD + "a menaquinol"
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PseudoCyc/BioCyc Reactions:
Thumb+Acceptor ↔ Thumb+Reduced acceptor
Ubiquinol-8 + Acceptor ↔ Ubiquinone-1 + Reduced acceptor
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Thumb+Thumb → Thumb+Thumb
NADH + Quinone → NAD + Hydroquinone
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Thumb+Thumb → Thumb+Thumb
NADH + Quinone → NAD + Hydroquinone
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Thumb+Thumb ↔ Thumb+Thumb
NADH + Ubiquinone-1 ↔ NAD + Ubiquinol-8
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Thumb+5 Thumb+Thumb → Thumb+Thumb+Thumb
NADH + 5 Hydrogen ion + Ubiquinone-1 → Hydrogen ion + NAD + Ubiquinol-1
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Thumb+4 Thumb+2 Thumb+menaquinone-8 ? Thumb+Thumb+Thumb+Electron+4 Thumb
NADH + 4 Hydrogen ion + 2 Hydrogen ion + menaquinone-8 ? NAD + Hydrogen ion + Menaquinol 8 + Electron + 4 Hydrogen ion
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Thumb
NADH + an oxidized unknown electron acceptor + H+ = NAD+ + an reduced unknown electron acceptor
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PAMDB110540+PAMDB001633+ETR-Quinones → PAMDB110542+ETR-Quinols
NADH + Hydrogen ion + ETR-Quinones → NAD+ + ETR-Quinols
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PAMDB000610+PAMDB110540+PAMDB001633 → PAMDB120506+PAMDB110542
Menaquinone + NADH + Hydrogen ion → menadiol + NAD+
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Complex Reactions:
Thumb+4 Thumb+Thumb → Thumb+Thumb+3 Thumb
2-Demethylmenaquinone 8 + 4 Hydrogen ion + NADH → 2-Demethylmenaquinol 8 + NAD + 3 Hydrogen ion
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4 Thumb+Thumb+Thumb → Thumb+Thumb+3 Thumb
4 Hydrogen ion + NADH + Ubiquinone-8 → NAD + Ubiquinol-8 + 3 Hydrogen ion
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4 Thumb+Menaquinone 8+Thumb → Thumb+Thumb+3 Thumb
4 Hydrogen ion + Menaquinone 8 + NADH → Menaquinol 8 + NAD + 3 Hydrogen ion
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Thumb+Thumb → Thumb+Thumb
NADH + Quinone → NAD + Hydroquinone
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Thumb+Thumb → Thumb+Thumb
NADH + Quinone → NAD + Hydroquinone
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Transports: Not Available
Metabolites:
PAMDB IDNameView
PAMDB0015632-Demethylmenaquinol 8MetaboCard
PAMDB0015642-Demethylmenaquinone 8MetaboCard
PAMDB001633Hydrogen ionMetaboCard
PAMDB000459HydroquinoneMetaboCard
PAMDB001653Menaquinol 8MetaboCard
PAMDB000197NADMetaboCard
PAMDB000398NADHMetaboCard
PAMDB003450QuinoneMetaboCard
PAMDB001968Ubiquinol-1MetaboCard
PAMDB000237Ubiquinol-8MetaboCard
PAMDB001833Ubiquinone-1MetaboCard
PAMDB001700Ubiquinone-8MetaboCard
GO Classification:
Function
catalytic activity
NADH dehydrogenase (quinone) activity
NADH dehydrogenase (ubiquinone) activity
NADH dehydrogenase activity
oxidoreductase activity
oxidoreductase activity, acting on NADH or NADPH
Process
ATP synthesis coupled electron transport
cellular metabolic process
electron transport chain
generation of precursor metabolites and energy
metabolic process
oxidation reduction
respiratory electron transport chain
Gene Properties
Locus tag: PA2648
Strand: +
Entrez Gene ID: 882357
Accession: NP_251338.1
GI: 15597844
Sequence start: 2994728
Sequence End: 2996257
Sequence Length: 1529
Gene Sequence: 
>PA2648
ATGATTCTGCCCTGGCTAATCCTGATCCCCTTCATCGGCGGTTTCCTCTGCTGGATCGCCGAACACAGCAGCAAGACCCTGCCCCGCTGGATCGCGCTGCTTTCGATGACCCTGGTCCTGATCCTGAGCCTGTGGATCTGGGCCACCGGCGACTTCCAGCTGGCGCCCGCGCCCGGCGGCGAGCCGGAATGGACCCTGCAATTCAAGGTGCTGTGGATCGAGCGTCTCGGCATCAGCATCCACCTGGCGATGGATGGCCTGTCGCTGCTGATGGTCGCCCTCACCGGCCTGCTCGGCGTGCTCTCGGTCCTCTGCTCCTGGAACGAGATCCAGCGCCGCATCGGCTTCTTCCATCTGAACCTGCTGTGGATCCTGGGCGGTGTGATCGGCGTGTTCCTCGCCATCGACCTGTTCCTGTTCTTCTTCTTCTGGGAAATGATGCTGGTGCCGATGTACTTCCTCATCGCGCTGTGGGGACACAGTTCCGATGACGGCAAGAAGACCCGCATCTACGCCGCCACCAAGTTCTTCATCTTCACCCAGGCCAGCGGCCTGGTGATGCTGGTGGCGATCCTCGGCCTGGTGTTCGTCAACTTCAATGCCACCGGCGTGATCACCTTCGACTACGCCACCCTGCTGAAGACCCAGCTGAGCCCCCACGTGGAATGGCTGCTGATGCTCGGCTTCTTCGTCGCCTTCGCGGTGAAGATGCCGGTGGTGCCGGTGCATTCCTGGCTGCCGGACGCCCACGCCCAGGCGCCCACCGCCGGCTCCGTGGACCTCGCCGGCATCCTCCTGAAGACCGCCGCCTACGGCCTGATCCGCTTCGCCCTGCCGCTGTTCCCCAACGCCTCGGCGGAATTCGCGCCGATCGCCATGTGGCTCGGCATCATCGGCATCTTCTACGGTGCCCTGCTGTCCTTCGCGCAGACCGACATCAAGCGCCTGGTCGCCTACTCCAGCGTTTCGCACATGGGCTTCGTGATGATCGGCATCTACTCCGGCAGCCAGGTAGCGCTGCAGGGCGTGGTGGTGCAGATGATCGCCCACGGCCTCTCCGCCGCGGCGCTGTTCATCCTCTGCGGCCAGCTCTACGAGCGCCTGCATACCCGCGACATGCGCAAGATGGGCGGCCTGTGGTCGCGCATGCCCTACCTGCCGGCGATCAGCCTGTTCTTCGCCTCGGCGTCGCTGGGCCTGCCCGGTACCGGCAACTTCGTCGGGGAGTTCCTGATCCTGATCGGCGCCTTCAAGGTGGTCCCGGTGATCATCGTGATCGCCACCTTCGGCCTGGTCTTCGCCTCGGTCTACTCGCTGATCATGATCCACCGCGCCTACTTCGGCCCCAGCCAGTCCGACGAGCCGATCCTCGGCCTCGATGCCCGCGAACTGAGCATGGTGCTCGGCCTGGCGGTCCTGCTGGTCCTGCTCGGGGTCTACCCGCAGCCAGTGCTGGACATCTCCGCGGCCAGCATGCACGGCGTGCAGCAATGGCTGGGCGCAGCCCTTTCCACCCTTGCAGGACGGTAG
Protein Properties
Protein Residues: 509
Protein Molecular Weight: 55.7 kDa
Protein Theoretical pI: 7.75
Hydropathicity (GRAVY score): 0.948
Charge at pH 7 (predicted): 2.78
Protein Sequence: 
>PA2648
MILPWLILIPFIGGFLCWIAEHSSKTLPRWIALLSMTLVLILSLWIWATGDFQLAPAPGGEPEWTLQFKVLWIERLGISIHLAMDGLSLLMVALTGLLGVLSVLCSWNEIQRRIGFFHLNLLWILGGVIGVFLAIDLFLFFFFWEMMLVPMYFLIALWGHSSDDGKKTRIYAATKFFIFTQASGLVMLVAILGLVFVNFNATGVITFDYATLLKTQLSPHVEWLLMLGFFVAFAVKMPVVPVHSWLPDAHAQAPTAGSVDLAGILLKTAAYGLIRFALPLFPNASAEFAPIAMWLGIIGIFYGALLSFAQTDIKRLVAYSSVSHMGFVMIGIYSGSQVALQGVVVQMIAHGLSAAALFILCGQLYERLHTRDMRKMGGLWSRMPYLPAISLFFASASLGLPGTGNFVGEFLILIGAFKVVPVIIVIATFGLVFASVYSLIMIHRAYFGPSQSDEPILGLDARELSMVLGLAVLLVLLGVYPQPVLDISAASMHGVQQWLGAALSTLAGR
References
External Links:
Resource Link
Genome ID: PA2648
Entrez Gene ID: 882357
NCBI Protein ID: 15597844
General Reference: PaperBLAST - Find papers about PA2648 and its homologs