NADH-quinone oxidoreductase subunit K (PA2646)

Identification
Name: NADH-quinone oxidoreductase subunit K
Synonyms:
  • NADH dehydrogenase I subunit K
  • NDH-1 subunit K
  • NUO11
Gene Name: nuoK
Enzyme Class:
Biological Properties
General Function: Involved in oxidoreductase activity, acting on NADH or NADPH
Specific Function: There are 2 NADH dehydrogenases in E.coli, however only this complex is able to use dNADH (reduced nicotinamide hypoxanthine dinucleotide, deamino-NADH) and dNADH-DB (dimethoxy- 5-methyl-6-decyl-1,4-benzoquinone) as substrates
Cellular Location: Cell inner membrane; Multi-pass membrane protein
KEGG Pathways:
KEGG Reactions:
Thumb+Acceptor ↔ Thumb+Reduced acceptor
Ubiquinol-8 + Acceptor ↔ Ubiquinone-1 + Reduced acceptor
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Thumb+Thumb ↔ Thumb+Thumb
NADH + Ubiquinone-1 ↔ NAD + Ubiquinol-8
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SMPDB Reactions:
Thumb+5 Thumb+Thumb → Thumb+Thumb+Thumb
NADH + 5 Hydrogen ion + Ubiquinone-1 → Hydrogen ion + NAD + Ubiquinol-1
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Thumb+4 Thumb+2 Thumb+menaquinone-8 ? Thumb+Thumb+Thumb+Electron+4 Thumb
NADH + 4 Hydrogen ion + 2 Hydrogen ion + menaquinone-8 ? NAD + Hydrogen ion + Menaquinol 8 + Electron + 4 Hydrogen ion
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"a menaquinone"+4 Thumb+Thumb → "a menaquinol"+Thumb
"a menaquinone" + 4 Hydrogen ion + NADH → "a menaquinol" + NAD
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4 Thumb+Thumb+"a menaquinone" → Thumb+"a menaquinol"
4 Hydrogen ion + NADH + "a menaquinone" → NAD + "a menaquinol"
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PseudoCyc/BioCyc Reactions:
Thumb+Acceptor ↔ Thumb+Reduced acceptor
Ubiquinol-8 + Acceptor ↔ Ubiquinone-1 + Reduced acceptor
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Thumb+Thumb → Thumb+Thumb
NADH + Quinone → NAD + Hydroquinone
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Thumb+Thumb → Thumb+Thumb
NADH + Quinone → NAD + Hydroquinone
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Thumb+Thumb → Thumb+Thumb
NADH + Quinone → NAD + Hydroquinone
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Thumb+Thumb → Thumb+Thumb
NADH + Quinone → NAD + Hydroquinone
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Thumb+Thumb ↔ Thumb+Thumb
NADH + Ubiquinone-1 ↔ NAD + Ubiquinol-8
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Thumb+5 Thumb+Thumb → Thumb+Thumb+Thumb
NADH + 5 Hydrogen ion + Ubiquinone-1 → Hydrogen ion + NAD + Ubiquinol-1
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Thumb+4 Thumb+2 Thumb+menaquinone-8 ? Thumb+Thumb+Thumb+Electron+4 Thumb
NADH + 4 Hydrogen ion + 2 Hydrogen ion + menaquinone-8 ? NAD + Hydrogen ion + Menaquinol 8 + Electron + 4 Hydrogen ion
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Thumb
NADH + an oxidized unknown electron acceptor + H+ = NAD+ + an reduced unknown electron acceptor
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PAMDB110540+PAMDB001633+ETR-Quinones → PAMDB110542+ETR-Quinols
NADH + Hydrogen ion + ETR-Quinones → NAD+ + ETR-Quinols
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PAMDB000610+PAMDB110540+PAMDB001633 → PAMDB120506+PAMDB110542
Menaquinone + NADH + Hydrogen ion → menadiol + NAD+
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Complex Reactions:
Thumb+4 Thumb+Thumb → Thumb+Thumb+3 Thumb
2-Demethylmenaquinone 8 + 4 Hydrogen ion + NADH → 2-Demethylmenaquinol 8 + NAD + 3 Hydrogen ion
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4 Thumb+Thumb+Thumb → Thumb+Thumb+3 Thumb
4 Hydrogen ion + NADH + Ubiquinone-8 → NAD + Ubiquinol-8 + 3 Hydrogen ion
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4 Thumb+Menaquinone 8+Thumb → Thumb+Thumb+3 Thumb
4 Hydrogen ion + Menaquinone 8 + NADH → Menaquinol 8 + NAD + 3 Hydrogen ion
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Thumb+Thumb → Thumb+Thumb
NADH + Quinone → NAD + Hydroquinone
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Thumb+Thumb → Thumb+Thumb
NADH + Quinone → NAD + Hydroquinone
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Thumb+Thumb → Thumb+Thumb
NADH + Quinone → NAD + Hydroquinone
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Thumb+Thumb → Thumb+Thumb
NADH + Quinone → NAD + Hydroquinone
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Transports: Not Available
Metabolites:
PAMDB IDNameView
PAMDB0015632-Demethylmenaquinol 8MetaboCard
PAMDB0015642-Demethylmenaquinone 8MetaboCard
PAMDB001633Hydrogen ionMetaboCard
PAMDB000459HydroquinoneMetaboCard
PAMDB001653Menaquinol 8MetaboCard
PAMDB000197NADMetaboCard
PAMDB000398NADHMetaboCard
PAMDB003450QuinoneMetaboCard
PAMDB001968Ubiquinol-1MetaboCard
PAMDB000237Ubiquinol-8MetaboCard
PAMDB001833Ubiquinone-1MetaboCard
PAMDB001700Ubiquinone-8MetaboCard
GO Classification:
Function
catalytic activity
oxidoreductase activity
oxidoreductase activity, acting on NADH or NADPH
Process
ATP synthesis coupled electron transport
cellular metabolic process
electron transport chain
generation of precursor metabolites and energy
metabolic process
oxidation reduction
respiratory electron transport chain
Gene Properties
Locus tag: PA2646
Strand: +
Entrez Gene ID: 882355
Accession: NP_251336.1
GI: 15597842
Sequence start: 2992548
Sequence End: 2992856
Sequence Length: 308
Gene Sequence: 
>PA2646
ATGAACGCAATACCACTGGAACACGGCCTGGCCCTCGCCAGCGTCCTGTTCGCCCTCGGACTGGTCGGCTTGATGGTGCGACGCAACATCCTGTTCGTACTGATGAGCCTGGAAGTGATGATGAACGCCGCCGCCCTCGCCTTCGTGGTCGCCGGCAGCCGCTGGGGCCAACCCGACGGCCAGGTGATGTTCATCCTGGTGCTGAGCCTCGCGGCCGCCGAAGCGAGCATCGGCCTGGCGATCCTGCTGCAACTGTATCGCCGCTTCCACACCCTCGATATCGACGCTGCCAGCGAGATGCGCGGATGA
Protein Properties
Protein Residues: 102
Protein Molecular Weight: 11 kDa
Protein Theoretical pI: 6.51
Hydropathicity (GRAVY score): 0.981
Charge at pH 7 (predicted): -0.54
Protein Sequence: 
>PA2646
MNAIPLEHGLALASVLFALGLVGLMVRRNILFVLMSLEVMMNAAALAFVVAGSRWGQPDGQVMFILVLSLAAAEASIGLAILLQLYRRFHTLDIDAASEMRG
References
External Links:
Resource Link
Genome ID: PA2646
Entrez Gene ID: 882355
NCBI Protein ID: 15597842
General Reference: PaperBLAST - Find papers about PA2646 and its homologs