Identification
Name: Respiratory nitrate reductase 1 gamma chain
Synonyms:
  • Cytochrome B-NR
  • Nitrate reductase A subunit gamma
  • Quinol-nitrate oxidoreductase subunit gamma
Gene Name: narI
Enzyme Class:
Biological Properties
General Function: Involved in nitrate reductase activity
Specific Function: The nitrate reductase enzyme complex allows E.coli to use nitrate as an electron acceptor during anaerobic growth. The gamma chain is a membrane-embedded heme-iron unit resembling cytochrome b, which transfers electrons from quinones to the beta subunit
Cellular Location: Cell inner membrane; Multi-pass membrane protein
KEGG Pathways:
KEGG Reactions:
Thumb+Acceptor+Thumb+AcceptorThumb+Reduced acceptor+Reduced acceptor
Nitrite + Acceptor + Water + Acceptor ↔ Nitrate + Reduced acceptor + Reduced acceptor
ReactionCard
2 Ferricytochrome c+Thumb+ThumbThumb+2 Ferrocytochrome c+2 Thumb
2 Ferricytochrome c + Nitrite + WaterNitrate + 2 Ferrocytochrome c + 2 Hydrogen ion
ReactionCard
SMPDB Reactions:
Nitrate+cytochrome c nitrite reductase+ThumbNitrite+cytochrome c nitrite reductase+Thumb+Thumb
Nitrate + cytochrome c nitrite reductase + Nitrate ↔ Nitrite + cytochrome c nitrite reductase + Water + Nitrite
ReactionCard
Thumb+2 Thumb+2 ElectronThumb+Thumb
Thumb+2 Thumb+"a menaquinol"Thumb+Thumb+"a menaquinone"
Nitrate + 2 Hydrogen ion + "a menaquinol" → Nitrite + Water + "a menaquinone"
ReactionCard
PseudoCyc/BioCyc Reactions:
Thumb+Acceptor+Thumb+AcceptorThumb+Reduced acceptor+Reduced acceptor
Nitrite + Acceptor + Water + Acceptor ↔ Nitrate + Reduced acceptor + Reduced acceptor
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2 Ferricytochrome c+Thumb+ThumbThumb+2 Ferrocytochrome c+2 Thumb
2 Ferricytochrome c + Nitrite + WaterNitrate + 2 Ferrocytochrome c + 2 Hydrogen ion
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Thumb+acceptorThumb+reduced acceptor
Nitrite + acceptor → Nitrate + reduced acceptor
ReactionCard
Nitrate+cytochrome c nitrite reductase+ThumbNitrite+cytochrome c nitrite reductase+Thumb+Thumb
Nitrate + cytochrome c nitrite reductase + Nitrate ↔ Nitrite + cytochrome c nitrite reductase + Water + Nitrite
ReactionCard
Thumb+2 Thumb+2 ElectronThumb+Thumb
Thumb
nitrite + an oxidized unknown electron acceptor + H2O = nitrate + an reduced unknown electron acceptor
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Menaquinones+PAMDB001633Menaquinols
Menaquinones + Hydrogen ion → Menaquinols
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PAMDB000468+PAMDB001633PAMDB000465+PAMDB000142
PAMDB000468PAMDB000465
PAMDB000468+UbiquinolsPAMDB000465+PAMDB000142+Ubiquinones
Nitrate + Ubiquinols → Nitrite + Water + Ubiquinones
ReactionCard
PAMDB000468+PAMDB001633+MenaquinolsPAMDB000465+PAMDB000142+Menaquinones+PAMDB001633
Nitrate + Hydrogen ion + Menaquinols → Nitrite + Water + Menaquinones + Hydrogen ion
ReactionCard
Complex Reactions:
2 Thumb+Thumb+ThumbThumb+Thumb+Thumb+2 Thumb
2 Thumb+Thumb+ThumbThumb+Menaquinone 8+Thumb+2 Thumb
Thumb+acceptorThumb+reduced acceptor
Nitrite + acceptor → Nitrate + reduced acceptor
ReactionCard
Transports: Not Available
Metabolites:
PAMDB IDNameView
PAMDB002046Ferricytochrome cMetaboCard
PAMDB003525Ferrocytochrome cMetaboCard
PAMDB001633Hydrogen ionMetaboCard
PAMDB001653Menaquinol 8MetaboCard
PAMDB000468NitrateMetaboCard
PAMDB000465NitriteMetaboCard
PAMDB004318SelenateMetaboCard
PAMDB001752SeleniteMetaboCard
PAMDB000237Ubiquinol-8MetaboCard
PAMDB001700Ubiquinone-8MetaboCard
PAMDB000142WaterMetaboCard
GO Classification:
Component
macromolecular complex
nitrate reductase complex
protein complex
Function
catalytic activity
nitrate reductase activity
oxidoreductase activity
oxidoreductase activity, acting on other nitrogenous compounds as donors
Process
metabolic process
oxidation reduction
Gene Properties
Locus tag: PA3872
Strand: -
Entrez Gene ID: 878884
Accession: NP_252561.1
GI: 15599067
Sequence start: 4335276
Sequence End: 4335959
Sequence Length: 683
Gene Sequence:
>PA3872
ATGTCGACCAATCTTCTGTTCTTCGGGATCTATCCCTATGTCGCGCTGCTGATCTGCCTGGTCGGCAGCTGGGCGCGCTTCGACCTCTCGCAGTACACCTGGAAGGCCGGTTCCAGCCAGATGCTCAGCAAGAAGGGCATGCGGGTATACAGCAACCTATTCCACGTCGGCGTGCTGTTCATCCTCGCCGGCCACTTCGTCGGCCTGCTGACCCCGGCCTCGGTCTACCACCACCTGATCAGCACCGAGAACAAGCAACTGCTGGCGATGGTCTCCGGCGGCTTCTTCGGCGTGCTCTGCTTCATCGGCCTGAGCGGACTGATCCTGCGCCGCCTGACCGACGCACGGGTGCGCGCCACCGGCAACGCCTCCGACCTGATGATCCTGCTGGTGCTCTACGCCCAGCTGATCCTCGGCCTCTCCACCATCGTCGCCTCGACCCATCACATGGATGGCTCGGTGATGGTGATGCTCGCCGAATGGGCCCAGGCCATCGTCACCCTGCGTCCGCTGGCGGCGGCCGAGGCCATCGCGCCGGTGGGCCTGGTCTACAAGCTGCACGTCGGCCTGGGCCTTACCCTGTTCGTGCTGTTCCCCTTCACCCGCCTGGTGCACATCGTCAGCGCGCCGGTGTGGTACCTGGGCCGGCGCTACCAGATCGTGCGTCAGAAACGTCCTGCCTGA
Protein Properties
Protein Residues: 227
Protein Molecular Weight: 25 kDa
Protein Theoretical pI: 10.43
Hydropathicity (GRAVY score): 0.751
Charge at pH 7 (predicted): 12.84
Protein Sequence:
>PA3872
MSTNLLFFGIYPYVALLICLVGSWARFDLSQYTWKAGSSQMLSKKGMRVYSNLFHVGVLFILAGHFVGLLTPASVYHHLISTENKQLLAMVSGGFFGVLCFIGLSGLILRRLTDARVRATGNASDLMILLVLYAQLILGLSTIVASTHHMDGSVMVMLAEWAQAIVTLRPLAAAEAIAPVGLVYKLHVGLGLTLFVLFPFTRLVHIVSAPVWYLGRRYQIVRQKRPA
References
External Links:
Resource Link
Genome ID: PA3872
Entrez Gene ID: 878884
NCBI Protein ID: 15599067
General Reference: PaperBLAST - Find papers about PA3872 and its homologs