Nitrite (PAMDB000465)

General function:

Involved in oxidation-reduction process

Specific function:

Ammonium hydroxide + 3 NAD(P)(+) + H(2)O = nitrite + 3 NAD(P)H

Gene Name:

nirB

Locus Tag:

PA1781

Molecular weight:

89.7 kDa

Reactions

General function:

Involved in oxidoreductase activity

Specific function:

The nitrate reductase enzyme complex allows Pseudomonas aeruginosa to use nitrate as an electron acceptor during anaerobic growth. The alpha chain is the actual site of nitrate reduction

Gene Name:

narG

Locus Tag:

PA3875

Molecular weight:

141 kDa

Reactions

General function:

Involved in nitrite reductase [NAD(P)H] activity

Specific function:

Required for activity of the reductase

Gene Name:

nirD

Locus Tag:

PA1780

Molecular weight:

11.6 kDa

Reactions

General function:

Involved in iron-sulfur cluster binding

Specific function:

The nitrate reductase enzyme complex allows Pseudomonas aeruginosa to use nitrate as an electron acceptor during anaerobic growth. The beta chain is an electron transfer unit containing four cysteine clusters involved in the formation of iron-sulfur centers. Electrons are transferred from the gamma chain to the molybdenum cofactor of the alpha subunit

Gene Name:

narH

Locus Tag:

PA3874

Molecular weight:

58.1 kDa

Reactions

General function:

Involved in nitrate reductase activity

Specific function:

The nitrate reductase enzyme complex allows Pseudomonas aeruginosa to use nitrate as an electron acceptor during anaerobic growth. The gamma chain is a membrane-embedded heme-iron unit resembling cytochrome b, which transfers electrons from quinones to the beta subunit

Gene Name:

narI

Locus Tag:

PA3872

Molecular weight:

25 kDa

Reactions

General function:

Involved in oxidoreductase activity

Specific function:

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein napC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism

Gene Name:

napA

Locus Tag:

PA1174

Molecular weight:

92.9 kDa

Reactions

General function:

Energy production and conversion

Specific function:

Small subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme napC protein, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism

Gene Name:

napB

Locus Tag:

PA1173

Molecular weight:

17.9 kDa

General function:

Involved in heme binding

Specific function:

Mediates electron flow from quinones to the napAB complex

Gene Name:

napC

Locus Tag:

PA1172

Molecular weight:

22.7 kDa

General function:

Involved in unfolded protein binding

Specific function:

Chaperone required for proper molybdenum cofactor insertion and final assembly of the membrane-bound respiratory nitrate reductase 1. Required for the insertion of the molybdenum into the apo-NarG subunit, maybe by keeping NarG in an appropriate competent-open conformation for the molybdenum cofactor insertion to occur. NarJ maintains the apoNarGH complex in a soluble state. Upon insertion of the molybdenum cofactor, NarJ seems to dissociate from the activated soluble NarGH complex, before its association with the NarI subunit on the membrane

Gene Name:

narJ

Locus Tag:

PA3873

Molecular weight:

27.3 kDa