Identification
Name: Respiratory nitrate reductase 1 alpha chain
Synonyms:
  • Nitrate reductase A subunit alpha
  • Quinol-nitrate oxidoreductase subunit alpha
Gene Name: narG
Enzyme Class:
Biological Properties
General Function: Involved in oxidoreductase activity
Specific Function: The nitrate reductase enzyme complex allows E.coli to use nitrate as an electron acceptor during anaerobic growth. The alpha chain is the actual site of nitrate reduction
Cellular Location: Cell membrane; Peripheral membrane protein
KEGG Pathways:
KEGG Reactions:
Thumb+Acceptor+Thumb+AcceptorThumb+Reduced acceptor+Reduced acceptor
Nitrite + Acceptor + Water + Acceptor ↔ Nitrate + Reduced acceptor + Reduced acceptor
ReactionCard
2 Ferricytochrome c+Thumb+ThumbThumb+2 Ferrocytochrome c+2 Thumb
2 Ferricytochrome c + Nitrite + WaterNitrate + 2 Ferrocytochrome c + 2 Hydrogen ion
ReactionCard
SMPDB Reactions:
Nitrate+cytochrome c nitrite reductase+ThumbNitrite+cytochrome c nitrite reductase+Thumb+Thumb
Nitrate + cytochrome c nitrite reductase + Nitrate ↔ Nitrite + cytochrome c nitrite reductase + Water + Nitrite
ReactionCard
Thumb+2 Thumb+2 ElectronThumb+Thumb
Thumb+2 Thumb+"a menaquinol"Thumb+Thumb+"a menaquinone"
Nitrate + 2 Hydrogen ion + "a menaquinol" → Nitrite + Water + "a menaquinone"
ReactionCard
PseudoCyc/BioCyc Reactions:
Thumb+Acceptor+Thumb+AcceptorThumb+Reduced acceptor+Reduced acceptor
Nitrite + Acceptor + Water + Acceptor ↔ Nitrate + Reduced acceptor + Reduced acceptor
ReactionCard
2 Ferricytochrome c+Thumb+ThumbThumb+2 Ferrocytochrome c+2 Thumb
2 Ferricytochrome c + Nitrite + WaterNitrate + 2 Ferrocytochrome c + 2 Hydrogen ion
ReactionCard
Thumb+acceptorThumb+reduced acceptor
Nitrite + acceptor → Nitrate + reduced acceptor
ReactionCard
Nitrate+cytochrome c nitrite reductase+ThumbNitrite+cytochrome c nitrite reductase+Thumb+Thumb
Nitrate + cytochrome c nitrite reductase + Nitrate ↔ Nitrite + cytochrome c nitrite reductase + Water + Nitrite
ReactionCard
Thumb+2 Thumb+2 ElectronThumb+Thumb
Thumb
nitrite + an oxidized unknown electron acceptor + H2O = nitrate + an reduced unknown electron acceptor
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Menaquinones+PAMDB001633Menaquinols
Menaquinones + Hydrogen ion → Menaquinols
ReactionCard
PAMDB000468+PAMDB001633PAMDB000465+PAMDB000142
PAMDB000468PAMDB000465
PAMDB000468+UbiquinolsPAMDB000465+PAMDB000142+Ubiquinones
Nitrate + Ubiquinols → Nitrite + Water + Ubiquinones
ReactionCard
PAMDB000468+PAMDB001633+MenaquinolsPAMDB000465+PAMDB000142+Menaquinones+PAMDB001633
Nitrate + Hydrogen ion + Menaquinols → Nitrite + Water + Menaquinones + Hydrogen ion
ReactionCard
Complex Reactions:
2 Thumb+Thumb+ThumbThumb+Thumb+Thumb+2 Thumb
2 Thumb+Thumb+ThumbThumb+Menaquinone 8+Thumb+2 Thumb
Thumb+acceptorThumb+reduced acceptor
Nitrite + acceptor → Nitrate + reduced acceptor
ReactionCard
Transports: Not Available
Metabolites:
PAMDB IDNameView
PAMDB002046Ferricytochrome cMetaboCard
PAMDB003525Ferrocytochrome cMetaboCard
PAMDB001633Hydrogen ionMetaboCard
PAMDB001653Menaquinol 8MetaboCard
PAMDB000468NitrateMetaboCard
PAMDB000465NitriteMetaboCard
PAMDB004318SelenateMetaboCard
PAMDB001752SeleniteMetaboCard
PAMDB000237Ubiquinol-8MetaboCard
PAMDB001700Ubiquinone-8MetaboCard
PAMDB000142WaterMetaboCard
GO Classification:
Component
macromolecular complex
nitrate reductase complex
protein complex
Function
binding
catalytic activity
cation binding
electron carrier activity
ion binding
metal ion binding
molybdenum ion binding
nitrate reductase activity
oxidoreductase activity
oxidoreductase activity, acting on other nitrogenous compounds as donors
transition metal ion binding
Process
metabolic process
nitrate metabolic process
nitrogen compound metabolic process
oxidation reduction
Gene Properties
Locus tag: PA3875
Strand: -
Entrez Gene ID: 879780
Accession: NP_252564.1
GI: 15599070
Sequence start: 4338261
Sequence End: 4342046
Sequence Length: 3785
Gene Sequence:
>PA3875
ATGAGTCACCTGCTCGACCGCCTGCAGTTCTTCAAGAAGAAGCAGGGCGAATTCGCCGATGGCCACGGCGAGACCAGCAACGAGAGCCGCGCCTGGGAAGGTGCCTACCGGCAGCGCTGGCAGCACGACAAGATCGTGCGCTCCACCCACGGGGTGAACTGCACCGGCTCCTGCTCCTGGAAGATCTACGTGAAGAACGGCCTGATCACCTGGGAAACCCAGCAGACCGACTACCCGCGCACCCGTCCGGACCTGCCCAACCACGAGCCGCGCGGCTGCCCGCGCGGGGCCAGCTATTCCTGGTACATCTACAGCGCCAACCGCCTGAAGTACCCGAAGGTGCGCAAGCCGTTGCTCAAGCTCTGGCGCGAGGCGCGGGCGCAGCACGGCGACCCGGTGAACGCCTGGGCCAGCATCGTCGAGGACGCCGCCAAGGCGAAGAGCTACAAGAGCCAGCGCGGCCTGGGTGGCTTCGTCCGTTCCAGCTGGGACGAGGTCACCGAGATCATCGCCGCGGCCAACGTCTACACCGCCAAGACCTACGGTCCGGACCGGGTGATCGGCTTCTCGCCGATCCCGGCCATGTCGATGGTCAGCTACGCCGCCGGCGCCCGCTACCTGTCGCTGATCGGCGGGGTCTGCCTGAGCTTCTACGACTGGTACTGCGACCTGCCGCCGGCCAGCCCGCAGATCTGGGGCGAGCAGACCGACGTGCCGGAGTCGGCCGACTGGTACAACTCCAGCTACATCATCGCCTGGGGCTCCAACGTGCCGCAGACGCGGACCCCGGACGCGCACTTCTTCACCGAGGTGCGCTACAAGGGCACCAAGACCGTCTCCATCACCCCGGACTATTCCGAGGTGGCCAAGCTCACCGACCTCTGGCTCAACCCCAAGCAGGGCACCGACGCCGCGCTGGGCATGGCCTTCGGTCACGTGATCCTGAAGGAGTTCCACCTCGACCGGCCGAGCGCCTACTTCGTCGACTACTGCCGCCAGTACACCGACATGCCGATGCTGGTGTTGCTGGAAGAACACGCCGGCGGCGCGTTCAAGCCGACCCGCTACCTGCGCGCCGCCGACCTGGCGGACAACCTCGGCCAGGACAACAACCCCGAGTGGAAGACCATCGCCTACGACGAGCGCAGCGGCGGGCTGGTCTCGCCCACCGGCGCCATCGGCTACCGCTGGGGCGAGTCTGGCAAGTGGAACATCGCCGAGCTGGACGGCAGGAGCGGTGACCAGACGCGCCTGCAACTGTCGCTGCTCGATGGCCCGGAACATGCCTGCGAGGTGGCCTTCCCGTACTTCGCCGGGCAGGAGCACCCGCACTTCAAGGGCGTCGCCAACGACGAGGTACTGCTGCGCCGGGTGCCGTTCCGCGAGATCGTCGCGGCGGACGGCAAGCGCCTGCGGGTGGCCACCGTCTATGACCTGCAGATGGCCAACTACAGCATCGACCGCGGCCTGGGCGGCGACAACGTGGCGACCTCCTACGAGGACGCCGACACGCCCTATACCCCGGCCTGGCAGGAGCGCATCACCGGCGTCCCGGCGGCGCGCGCGACGCAGGTCGCCCGCGAGTTCGCCGACAGCGCCGACAAGACCCGCGGCAAGGCGATGGTGATCATCGGCGCGGCGATGAACCACTGGTACCACATGGACATGAACTACCGCGCGGTCATCAACATGCTGATGATGTGCGGCTGCATCGGCCAAAGCGGCGGCGGCTGGGCGCACTATGTCGGCCAGGAGAAGCTGCGCCCGCAGACCGGCTGGGCGCCGCTGGCCTTCGGCCTGGACTGGAGCCGGCCGCCGCGGCAGATGAACGGCACCAGCTTCTTCTACCTGCACAGCTCGCAATGGCGCCACGAGAAGCTGTCGATGCACGAGGTGCTGTCGCCGCTGGCCGACGCCAGCCGCTTCGCCGAACACGCCCTGGACTACAACATCCAGGCCGAACGCCTCGGCTGGCTGCCGTCGGCGCCGCAACTGAACCGCAACCCGCTGCGCATCGCCGCCGAGGCCGAGGCCGCCGGCCTGCCGGTCGCCGACTATGTGGTGCGTGAACTGAAGAGCGGCGGCCTGCGCTTCGCCAGCGAATCGCCGGACGATCCGCAGAACTTCCCGCGCAACATGTTCATCTGGCGCTCCAACCTGCTGGGCTCCTCCGGCAAGGGGCACGAGTACATGCTCAAGTACCTGCTCGGGGCGAAGAACGGGGTGATGAACGATGACCTCGGCAAGGCCGGCGGTCCGCGTCCCACCGAGGTCGACTGGGTCGACGACGGTGCCGAGGGCAAGCTCGACCTGGTCACCACCCTGGACTTCCGCATGTCCTCCACCTGCATGTACTCGGACATCGTCCTGCCGACCGCTACCTGGTACGAGAAGGACGACCTCAACACCTCCGACATGCACCCCTTCATCCATCCGCTGTCGGCGGCCACCGATCCGGCCTGGGAAGCCAAGAGCGACTGGGAGATCTACAAGGCCATCGCCAAGAAGTTCTCCGCCGTCGCCGAAGGCCACCTCGGCGTGGAGCAGGACCTGGTCACGGTGCCGCTGCTGCATGACACCCCCACCGAGCTGGCGCAGCCGTTCGGCGGCGACGGCCATGACTGGAAGAAGGGCGAGTGCGAGCCGGTGCCGGGACGCAACCTGCCGACGCTGCACCTGGTCGAGCGCGACTACCCGAACGTCTACCGCAAGTTCACCTCGCTCGGTCCGCTGCTGGACAAGCTGGGCAACGGCGGCAAGGGCATCGGCTGGAACACCGAGAAGGAAGTGAAGCTGGTCGGCGACCTCAACCATCGCGTGGTCGAGAGCGGCGTCAGCCAGGGCCGGCCGCGTATCGACAGCGCCATCGACGCCGCCGAGGTGGTCCTCGCCCTGGCTCCGGAAACCAACGGCCAGGTCGCGGTCAAGGCCTGGGAAGCGCTGTCGAAGATCACCGGCCGCGAGCACGCCCACCTGGCGCTGCCCAAGGAAGACGAGAAGATCCGCTTCCGCGACATCCAGGTGCAGCCGCGCAAGATCATCTCCAGCCCGACCTGGTCCGGCCTCGAGGACGAGCACGTCAGCTACAACGCCGGCTACACCAACGTCCACGAGCTGATCCCGTGGCGCACCATCACCGGTCGCCAGCAGTTCTACCAGGACCACCCGTGGATGCAGGCGTTCGGCGAAGGCTTCGTCAGCTACCGGCCGCCGGTCAACACCCGGACCACCGAGAAACTGTTGAACAGGAAGCCCAACGGCAACCCGGAGATCACCCTGAACTGGATCACCCCGCACCAGAAATGGGGCATCCACTCCACCTACAGCGACAACCTGCTGATGCTCACCCTGTCGCGCGGCGGTCCGATCATCTGGCTCAGCGAGCACGACGCGGCCAAGGCCGGGATCGTCGATAACGACTGGGTCGAGGTGTTCAACGCCAACGGCGCGGCGACCTGCCGCGCGGTGGTCAGCCAGCGGGTCAAGGACGGCATGGTGATGATGTACCACGCCCAGGAACGCATCGTGAACGTACCCGGCAGCGAGACCACCGGCACCCGTGGCGGCCACCACAACTCGGTGACCCGCGTGGTGCTCAAGCCGACCCACATGATCGGCGGCTACGCCCAGCAGGCCTGGGGCTTCAACTACTACGGCACGGTCGGCTGCAACCGCGACGAGTTCGTCGTGGTGCGCAAGATGAGCAAGGTCGACTGGCTGGACGAACCCCGCCACGGCGGACTCGGCGGCGACGCCCTGCCGCAACCGCTGCCCCAGGACATTTGA
Protein Properties
Protein Residues: 1261
Protein Molecular Weight: 141 kDa
Protein Theoretical pI: 6.74
Hydropathicity (GRAVY score): -0.482
Charge at pH 7 (predicted): -5.47
Protein Sequence:
>PA3875
MSHLLDRLQFFKKKQGEFADGHGETSNESRAWEGAYRQRWQHDKIVRSTHGVNCTGSCSWKIYVKNGLITWETQQTDYPRTRPDLPNHEPRGCPRGASYSWYIYSANRLKYPKVRKPLLKLWREARAQHGDPVNAWASIVEDAAKAKSYKSQRGLGGFVRSSWDEVTEIIAAANVYTAKTYGPDRVIGFSPIPAMSMVSYAAGARYLSLIGGVCLSFYDWYCDLPPASPQIWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGTKTVSITPDYSEVAKLTDLWLNPKQGTDAALGMAFGHVILKEFHLDRPSAYFVDYCRQYTDMPMLVLLEEHAGGAFKPTRYLRAADLADNLGQDNNPEWKTIAYDERSGGLVSPTGAIGYRWGESGKWNIAELDGRSGDQTRLQLSLLDGPEHACEVAFPYFAGQEHPHFKGVANDEVLLRRVPFREIVAADGKRLRVATVYDLQMANYSIDRGLGGDNVATSYEDADTPYTPAWQERITGVPAARATQVAREFADSADKTRGKAMVIIGAAMNHWYHMDMNYRAVINMLMMCGCIGQSGGGWAHYVGQEKLRPQTGWAPLAFGLDWSRPPRQMNGTSFFYLHSSQWRHEKLSMHEVLSPLADASRFAEHALDYNIQAERLGWLPSAPQLNRNPLRIAAEAEAAGLPVADYVVRELKSGGLRFASESPDDPQNFPRNMFIWRSNLLGSSGKGHEYMLKYLLGAKNGVMNDDLGKAGGPRPTEVDWVDDGAEGKLDLVTTLDFRMSSTCMYSDIVLPTATWYEKDDLNTSDMHPFIHPLSAATDPAWEAKSDWEIYKAIAKKFSAVAEGHLGVEQDLVTVPLLHDTPTELAQPFGGDGHDWKKGECEPVPGRNLPTLHLVERDYPNVYRKFTSLGPLLDKLGNGGKGIGWNTEKEVKLVGDLNHRVVESGVSQGRPRIDSAIDAAEVVLALAPETNGQVAVKAWEALSKITGREHAHLALPKEDEKIRFRDIQVQPRKIISSPTWSGLEDEHVSYNAGYTNVHELIPWRTITGRQQFYQDHPWMQAFGEGFVSYRPPVNTRTTEKLLNRKPNGNPEITLNWITPHQKWGIHSTYSDNLLMLTLSRGGPIIWLSEHDAAKAGIVDNDWVEVFNANGAATCRAVVSQRVKDGMVMMYHAQERIVNVPGSETTGTRGGHHNSVTRVVLKPTHMIGGYAQQAWGFNYYGTVGCNRDEFVVVRKMSKVDWLDEPRHGGLGGDALPQPLPQDI
References
External Links:
Resource Link
Genome ID: PA3875
Entrez Gene ID: 879780
NCBI Protein ID: 15599070
General Reference: PaperBLAST - Find papers about PA3875 and its homologs