Identification
Name: Nitrate reductase molybdenum cofactor assembly chaperone NarJ
Synonyms:
  • Redox enzyme maturation protein NarJ
Gene Name: narJ
Enzyme Class: Not Available
Biological Properties
General Function: Involved in unfolded protein binding
Specific Function: Chaperone required for proper molybdenum cofactor insertion and final assembly of the membrane-bound respiratory nitrate reductase 1. Required for the insertion of the molybdenum into the apo-NarG subunit, maybe by keeping NarG in an appropriate competent-open conformation for the molybdenum cofactor insertion to occur. NarJ maintains the apoNarGH complex in a soluble state. Upon insertion of the molybdenum cofactor, NarJ seems to dissociate from the activated soluble NarGH complex, before its association with the NarI subunit on the membrane
Cellular Location: Cytoplasm
KEGG Pathways:
KEGG Reactions:
Thumb+Acceptor+Thumb+AcceptorThumb+Reduced acceptor+Reduced acceptor
Nitrite + Acceptor + Water + Acceptor ↔ Nitrate + Reduced acceptor + Reduced acceptor
ReactionCard
2 Ferricytochrome c+Thumb+ThumbThumb+2 Ferrocytochrome c+2 Thumb
2 Ferricytochrome c + Nitrite + WaterNitrate + 2 Ferrocytochrome c + 2 Hydrogen ion
ReactionCard
SMPDB Reactions:
Nitrate+cytochrome c nitrite reductase+ThumbNitrite+cytochrome c nitrite reductase+Thumb+Thumb
Nitrate + cytochrome c nitrite reductase + Nitrate ↔ Nitrite + cytochrome c nitrite reductase + Water + Nitrite
ReactionCard
Thumb+2 Thumb+2 ElectronThumb+Thumb
Thumb+2 Thumb+"a menaquinol"Thumb+Thumb+"a menaquinone"
Nitrate + 2 Hydrogen ion + "a menaquinol" → Nitrite + Water + "a menaquinone"
ReactionCard
PseudoCyc/BioCyc Reactions:
Thumb+Acceptor+Thumb+AcceptorThumb+Reduced acceptor+Reduced acceptor
Nitrite + Acceptor + Water + Acceptor ↔ Nitrate + Reduced acceptor + Reduced acceptor
ReactionCard
2 Ferricytochrome c+Thumb+ThumbThumb+2 Ferrocytochrome c+2 Thumb
2 Ferricytochrome c + Nitrite + WaterNitrate + 2 Ferrocytochrome c + 2 Hydrogen ion
ReactionCard
Nitrate+cytochrome c nitrite reductase+ThumbNitrite+cytochrome c nitrite reductase+Thumb+Thumb
Nitrate + cytochrome c nitrite reductase + Nitrate ↔ Nitrite + cytochrome c nitrite reductase + Water + Nitrite
ReactionCard
Thumb+2 Thumb+2 ElectronThumb+Thumb
Thumb
nitrite + an oxidized unknown electron acceptor + H2O = nitrate + an reduced unknown electron acceptor
ReactionCard
Menaquinones+PAMDB001633Menaquinols
Menaquinones + Hydrogen ion → Menaquinols
ReactionCard
PAMDB000468+PAMDB001633PAMDB000465+PAMDB000142
PAMDB000468PAMDB000465
PAMDB000468+UbiquinolsPAMDB000465+PAMDB000142+Ubiquinones
Nitrate + Ubiquinols → Nitrite + Water + Ubiquinones
ReactionCard
PAMDB000468+PAMDB001633+MenaquinolsPAMDB000465+PAMDB000142+Menaquinones+PAMDB001633
Nitrate + Hydrogen ion + Menaquinols → Nitrite + Water + Menaquinones + Hydrogen ion
ReactionCard
Complex Reactions:
2 Thumb+Thumb+ThumbThumb+Thumb+Thumb+2 Thumb
2 Thumb+Thumb+ThumbThumb+Menaquinone 8+Thumb+2 Thumb
Transports: Not Available
Metabolites:
PAMDB IDNameView
PAMDB002046Ferricytochrome cMetaboCard
PAMDB003525Ferrocytochrome cMetaboCard
PAMDB001633Hydrogen ionMetaboCard
PAMDB001653Menaquinol 8MetaboCard
PAMDB000468NitrateMetaboCard
PAMDB000465NitriteMetaboCard
PAMDB004318SelenateMetaboCard
PAMDB001752SeleniteMetaboCard
PAMDB000237Ubiquinol-8MetaboCard
PAMDB001700Ubiquinone-8MetaboCard
PAMDB000142WaterMetaboCard
GO Classification:
Function
binding
protein binding
unfolded protein binding
Process
cellular component assembly
cellular component organization
cellular component organization or biogenesis
cellular protein complex assembly
chaperone-mediated protein complex assembly
macromolecular complex assembly
protein complex assembly
Gene Properties
Locus tag: PA3873
Strand: -
Entrez Gene ID: 879782
Accession: NP_252562.1
GI: 15599068
Sequence start: 4335962
Sequence End: 4336702
Sequence Length: 740
Gene Sequence:
>PA3873
ATGAACGATCACAGCCAACTGTTCCGCCTGCTCGCCCTGCTACTCGACTATCCACGCGCCGAGCTGCGCGAGGAGAGCCTCGGCCTGCATGCGCTGATCCGCACCTGCGAACTGCCGGAAGCGCTGCGCGACGGCCTCGCGGCGCTGCTCAACGAGCTCTGCCAGGGCGACCTGCTGGACGTCCAGGCGCGCTACGACGGTCTCTTCGAGCGCGGCCGCTCGGTCTCGCTGCTGCTCTTCGAGCACGTCCACGGCGAGAGCCGCGACCGTGGCCAGGCGATGGTCGACCTGCTCGACCGCTATACCGGGGCCGGCCTGCAGATCGACGTACCGGAGCTGCCGGACTACCTGCCGCTGTACCTCGAATACCTGTCGCTGCTGCCGTTCGCGGCGGCCAGCGAAGGGCTCGCCGAAGTGGCGCACATCCTCGGCCTGCTGGCGCTGCGCCTGGAGGAACGCGGCAGCGCCTACGCGGCGATTTTCGAGGCGTTGCTGGAACTCGGCGGCGAGCGCCCGGACCTCGGCGCGTTGCGTCGCGACCAGGCCCAGGAACAGCGCGACGACAGCCTGGAGGCCATCGACCGGGCCTGGGAGGAAACCCCGGTGAGCTTCACCGACCCCGCCGGCGGTTGCCCGTCGAGCAGCGGCCGCCGTCCAACGGCGTCCACCGAACAACCATTGCAATGGGTCGCCCAGCCGGTACCGCAGATGCAGTATCGAGCGGCCCGCGAAGGAGTCTGA
Protein Properties
Protein Residues: 246
Protein Molecular Weight: 27.3 kDa
Protein Theoretical pI: 4.31
Hydropathicity (GRAVY score): -0.214
Charge at pH 7 (predicted): -17.88
Protein Sequence:
>PA3873
MNDHSQLFRLLALLLDYPRAELREESLGLHALIRTCELPEALRDGLAALLNELCQGDLLDVQARYDGLFERGRSVSLLLFEHVHGESRDRGQAMVDLLDRYTGAGLQIDVPELPDYLPLYLEYLSLLPFAAASEGLAEVAHILGLLALRLEERGSAYAAIFEALLELGGERPDLGALRRDQAQEQRDDSLEAIDRAWEETPVSFTDPAGGCPSSSGRRPTASTEQPLQWVAQPVPQMQYRAAREGV
References
External Links:
Resource Link
Genome ID: PA3873
Entrez Gene ID: 879782
NCBI Protein ID: 15599068
General Reference: PaperBLAST - Find papers about PA3873 and its homologs