NADH-quinone oxidoreductase subunit I (PA2644)

Identification
Name: NADH-quinone oxidoreductase subunit I
Synonyms:
  • NADH dehydrogenase I subunit I
  • NDH-1 subunit I
  • NUO9
Gene Name: nuoI
Enzyme Class:
Biological Properties
General Function: Involved in electron carrier activity
Specific Function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient
Cellular Location: Cell inner membrane; Peripheral membrane protein (Potential)
KEGG Pathways:
KEGG Reactions:
Thumb+Acceptor ↔ Thumb+Reduced acceptor
Ubiquinol-8 + Acceptor ↔ Ubiquinone-1 + Reduced acceptor
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Thumb+Thumb ↔ Thumb+Thumb
NADH + Ubiquinone-1 ↔ NAD + Ubiquinol-8
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SMPDB Reactions:
Thumb+5 Thumb+Thumb → Thumb+Thumb+Thumb
NADH + 5 Hydrogen ion + Ubiquinone-1 → Hydrogen ion + NAD + Ubiquinol-1
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Thumb+4 Thumb+2 Thumb+menaquinone-8 ? Thumb+Thumb+Thumb+Electron+4 Thumb
NADH + 4 Hydrogen ion + 2 Hydrogen ion + menaquinone-8 ? NAD + Hydrogen ion + Menaquinol 8 + Electron + 4 Hydrogen ion
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"a menaquinone"+4 Thumb+Thumb → "a menaquinol"+Thumb
"a menaquinone" + 4 Hydrogen ion + NADH → "a menaquinol" + NAD
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4 Thumb+Thumb+"a menaquinone" → Thumb+"a menaquinol"
4 Hydrogen ion + NADH + "a menaquinone" → NAD + "a menaquinol"
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PseudoCyc/BioCyc Reactions:
Thumb+Acceptor ↔ Thumb+Reduced acceptor
Ubiquinol-8 + Acceptor ↔ Ubiquinone-1 + Reduced acceptor
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Thumb+Thumb → Thumb+Thumb
NADH + Quinone → NAD + Hydroquinone
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Thumb+Thumb → Thumb+Thumb
NADH + Quinone → NAD + Hydroquinone
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Thumb+Thumb → Thumb+Thumb
NADH + Quinone → NAD + Hydroquinone
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Thumb+Thumb → Thumb+Thumb
NADH + Quinone → NAD + Hydroquinone
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Thumb+Thumb → Thumb+Thumb
NADH + Quinone → NAD + Hydroquinone
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Thumb+Thumb → Thumb+Thumb
NADH + Quinone → NAD + Hydroquinone
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Thumb+Thumb ↔ Thumb+Thumb
NADH + Ubiquinone-1 ↔ NAD + Ubiquinol-8
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Thumb+5 Thumb+Thumb → Thumb+Thumb+Thumb
NADH + 5 Hydrogen ion + Ubiquinone-1 → Hydrogen ion + NAD + Ubiquinol-1
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Thumb+4 Thumb+2 Thumb+menaquinone-8 ? Thumb+Thumb+Thumb+Electron+4 Thumb
NADH + 4 Hydrogen ion + 2 Hydrogen ion + menaquinone-8 ? NAD + Hydrogen ion + Menaquinol 8 + Electron + 4 Hydrogen ion
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Thumb
NADH + an oxidized unknown electron acceptor + H+ = NAD+ + an reduced unknown electron acceptor
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PAMDB110540+PAMDB001633+ETR-Quinones → PAMDB110542+ETR-Quinols
NADH + Hydrogen ion + ETR-Quinones → NAD+ + ETR-Quinols
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PAMDB000610+PAMDB110540+PAMDB001633 → PAMDB120506+PAMDB110542
Menaquinone + NADH + Hydrogen ion → menadiol + NAD+
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Complex Reactions:
Thumb+4 Thumb+Thumb → Thumb+Thumb+3 Thumb
2-Demethylmenaquinone 8 + 4 Hydrogen ion + NADH → 2-Demethylmenaquinol 8 + NAD + 3 Hydrogen ion
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4 Thumb+Thumb+Thumb → Thumb+Thumb+3 Thumb
4 Hydrogen ion + NADH + Ubiquinone-8 → NAD + Ubiquinol-8 + 3 Hydrogen ion
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4 Thumb+Menaquinone 8+Thumb → Thumb+Thumb+3 Thumb
4 Hydrogen ion + Menaquinone 8 + NADH → Menaquinol 8 + NAD + 3 Hydrogen ion
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Thumb+Thumb → Thumb+Thumb
NADH + Quinone → NAD + Hydroquinone
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Thumb+Thumb → Thumb+Thumb
NADH + Quinone → NAD + Hydroquinone
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Thumb+Thumb → Thumb+Thumb
NADH + Quinone → NAD + Hydroquinone
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Thumb+Thumb → Thumb+Thumb
NADH + Quinone → NAD + Hydroquinone
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Thumb+Thumb → Thumb+Thumb
NADH + Quinone → NAD + Hydroquinone
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Thumb+Thumb → Thumb+Thumb
NADH + Quinone → NAD + Hydroquinone
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Transports: Not Available
Metabolites:
PAMDB IDNameView
PAMDB0015632-Demethylmenaquinol 8MetaboCard
PAMDB0015642-Demethylmenaquinone 8MetaboCard
PAMDB001633Hydrogen ionMetaboCard
PAMDB000459HydroquinoneMetaboCard
PAMDB001653Menaquinol 8MetaboCard
PAMDB000197NADMetaboCard
PAMDB000398NADHMetaboCard
PAMDB003450QuinoneMetaboCard
PAMDB001968Ubiquinol-1MetaboCard
PAMDB000237Ubiquinol-8MetaboCard
PAMDB001833Ubiquinone-1MetaboCard
PAMDB001700Ubiquinone-8MetaboCard
GO Classification:
Component
cell part
membrane
Function
4 iron, 4 sulfur cluster binding
binding
catalytic activity
electron carrier activity
iron-sulfur cluster binding
metal cluster binding
oxidoreductase activity
oxidoreductase activity, acting on NADH or NADPH
Process
metabolic process
oxidation reduction
Gene Properties
Locus tag: PA2644
Strand: +
Entrez Gene ID: 882353
Accession: NP_251334.1
GI: 15597840
Sequence start: 2991442
Sequence End: 2991990
Sequence Length: 548
Gene Sequence: 
>PA2644
GTGATCAAAGAAATCATCAACGTCGTGCATGGCACCTTCACCCAGCTTCGCAGCCTGGTGATGATCTTCGGCCATGCCTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAGCCGGTCTACCTGCCGCCGCGCTATCGTGGCCGGATCGTCCTGACCCGCGACCCCGACGGCGAGGAACGCTGCGTAGCCTGCAACCTGTGCGCCGTGGCCTGTCCGGTTGGCTGCATCTCCCTGCAGAAGGCCGAGACCGAGGACGGTCGCTGGTATCCGGAGTTCTTCCGCATCAACTTCTCGCGCTGCATCTTCTGCGGCCTGTGCGAGGAAGCCTGCCCGACCACCGCGATCCAGCTCACCCCGGACTTCGAGATGGGCGAGTTCAAGCGCCAGGACCTGGTCTACGAGAAGCACGACCTGCTGATCTCCGGGCCGGGCAAGAACCCGGACTACAACTACTACCGCGTTGCGGGGATGGCGATCGCCGGCAAGCCGAAAGGCGCCGCGCAGAACGAAGCCGAACCGATCAACGTGAAAAGCCTGCTGCCATAA
Protein Properties
Protein Residues: 182
Protein Molecular Weight: 20.6 kDa
Protein Theoretical pI: 6.72
Hydropathicity (GRAVY score): -0.238
Charge at pH 7 (predicted): -0.56
Protein Sequence: 
>PA2644
MIKEIINVVHGTFTQLRSLVMIFGHAFRKRDTLQYPEEPVYLPPRYRGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETEDGRWYPEFFRINFSRCIFCGLCEEACPTTAIQLTPDFEMGEFKRQDLVYEKHDLLISGPGKNPDYNYYRVAGMAIAGKPKGAAQNEAEPINVKSLLP
References
External Links:
Resource Link
Genome ID: PA2644
Entrez Gene ID: 882353
NCBI Protein ID: 15597840
General Reference: PaperBLAST - Find papers about PA2644 and its homologs