Formate dehydrogenase, nitrate-inducible, iron-sulfur subunit (PA4811)

Identification
Name: Formate dehydrogenase, nitrate-inducible, iron-sulfur subunit
Synonyms:
  • Anaerobic formate dehydrogenase iron-sulfur subunit
  • Formate dehydrogenase-N subunit beta
  • FDH-N subunit beta
Gene Name: fdnH
Enzyme Class: Not Available
Biological Properties
General Function: Involved in electron carrier activity
Specific Function: Formate dehydrogenase allows E.coli to use formate as major electron donor during anaerobic respiration, when nitrate is used as electron acceptor. The beta chain is an electron transfer unit containing 4 cysteine clusters involved in the formation of iron-sulfur centers. Electrons are transferred from the gamma chain to the molybdenum cofactor of the alpha subunit
Cellular Location: Cell membrane; Single-pass membrane protein
KEGG Pathways:
KEGG Reactions:
Thumb+Thumb ↔ Thumb+Thumb+Thumb
Formic acid + NAD ↔ Hydrogen ion + Carbon dioxide + NADH
ReactionCard
SMPDB Reactions: Not Available
PseudoCyc/BioCyc Reactions:
Thumb+Thumb ↔ Thumb+Thumb+Thumb
Formic acid + NAD ↔ Hydrogen ion + Carbon dioxide + NADH
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Thumb
formate[inner membrane] + NAD+[inner membrane] → CO2[inner membrane] + NADH[inner membrane] + H+[inner membrane]
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Thumb
a menaquinone[membrane] + 2 H+[in] + 2 e-[membrane] → a menaquinol[membrane]
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Thumb
CO2[out] + H+[out] + 2 e-[membrane] → formate[out]
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PAMDB110760+ETR-Quinones+PAMDB001633 → PAMDB000568+ETR-Quinols
formate + ETR-Quinones + Hydrogen ion → Carbon dioxide + ETR-Quinols
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Complex Reactions:
2 Thumb+Menaquinone 8+Thumb → Thumb+Thumb+Thumb
2 Hydrogen ion + Menaquinone 8 + Formic acid → Menaquinol 8 + Carbon dioxide + Hydrogen ion
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2 Thumb+Thumb+Thumb → Thumb+Thumb+Thumb
2 Hydrogen ion + Ubiquinone-8 + Formic acid → Ubiquinol-8 + Carbon dioxide + Hydrogen ion
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Transports: Not Available
Metabolites:
PAMDB IDNameView
PAMDB000568Carbon dioxideMetaboCard
PAMDB000053Formic acidMetaboCard
PAMDB001633Hydrogen ionMetaboCard
PAMDB001653Menaquinol 8MetaboCard
PAMDB000197NADMetaboCard
PAMDB000398NADHMetaboCard
PAMDB000237Ubiquinol-8MetaboCard
PAMDB001700Ubiquinone-8MetaboCard
GO Classification:
Component
cell part
integral to membrane
intrinsic to membrane
membrane part
Function
binding
catalytic activity
electron carrier activity
formate dehydrogenase activity
iron-sulfur cluster binding
metal cluster binding
oxidoreductase activity
oxidoreductase activity, acting on the aldehyde or oxo group of donors
oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
Process
cellular metabolic process
cellular respiration
energy derivation by oxidation of organic compounds
generation of precursor metabolites and energy
metabolic process
Gene Properties
Locus tag: PA4811
Strand: -
Entrez Gene ID: 880193
Accession: NP_253499.1
GI: 15600005
Sequence start: 5397690
Sequence End: 5398619
Sequence Length: 929
Gene Sequence: 
>PA4811
ATGAGTAGTCAGGACATCCACGCCCGCTCCGCGACCACCACGCCGATGCCCTCGGTGCGGAACCAGGAGGAAGTGGCGAAACTGATCGACGTGTCCAAGTGCATCGGTTGCAAGGCCTGCCAGGTCGCCTGCTCGGAATGGAACGAGCTGCGCGACGAGGTCGGCCACAACCACGGCACCTACGACAACCCCATGGACCTGACGGCCGACTCGTGGACGGTGATGCGTTTCACCGAGCACGAGAACGAGGCCGGCAACCTCGAGTGGCTGATCCGCAAGGATGGCTGCATGCACTGCGCCGAGCCGGGCTGCCTGAAGGCCTGCCCGAGCCCGGGAGCGATCGTGAAGTACGCCAACGGCATCGTCGACTTCAACCAGGACAAGTGCATCGGTTGCGGCTACTGCATCACCGGTTGCCCGTTCGACATCCCACGGATCTCGCAGAAGGACCACAAGGCCTACAAGTGCAGCCTGTGTTCCGACCGCGTCTCGGTGGGCATGGAGCCGGCCTGCGTGAAGACCTGCCCGACCGGCGCCATCGTCTTCGGCAGCAAGGAGGACATGAAGGAACACGCCGCCGAACGCATCGTCGACCTGAAGAGCCGCGGTTACGAGAACGCCGGCCTGTACGACCCCGAAGGGGTGGGCGGCACCCACGTGATGTACGTGCTGCACCACGCCGACAAGCCGTCGCTCTATGCCGGCCTGGCCGACCAGCCGAGCGTCAGCCCGCTGGTCAGCCTGTGGAAGGGCGTGACCAAGCCGCTGGCGCTGCTGGCCATGGGCGCCACGGTGCTCGCCGGGTTCTTCCACTATGTGCGCGTCGGTCCCAACCGCGCGGACGACGAGGAAGACCACGGGCAAGCCGAGGCACCGGTGCACCAGGTCGATCCGTCGGTACACACCTTTGACCCGCACGAGCGTCGTTGA
Protein Properties
Protein Residues: 309
Protein Molecular Weight: 33.8 kDa
Protein Theoretical pI: 5.97
Hydropathicity (GRAVY score): -0.359
Charge at pH 7 (predicted): -8.88
Protein Sequence: 
>PA4811
MSSQDIHARSATTTPMPSVRNQEEVAKLIDVSKCIGCKACQVACSEWNELRDEVGHNHGTYDNPMDLTADSWTVMRFTEHENEAGNLEWLIRKDGCMHCAEPGCLKACPSPGAIVKYANGIVDFNQDKCIGCGYCITGCPFDIPRISQKDHKAYKCSLCSDRVSVGMEPACVKTCPTGAIVFGSKEDMKEHAAERIVDLKSRGYENAGLYDPEGVGGTHVMYVLHHADKPSLYAGLADQPSVSPLVSLWKGVTKPLALLAMGATVLAGFFHYVRVGPNRADDEEDHGQAEAPVHQVDPSVHTFDPHERR
References
External Links:
Resource Link
Genome ID: PA4811
Entrez Gene ID: 880193
NCBI Protein ID: 15600005
General Reference: PaperBLAST - Find papers about PA4811 and its homologs