L-Methionine (PAMDB000175)

General function:

Involved in nucleotide binding

Specific function:

Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation

Gene Name:

metG

Locus Tag:

PA3482

Molecular weight:

74.9 kDa

Reactions

General function:

Involved in transferase activity

Specific function:

An aromatic amino acid + 2-oxoglutarate = an aromatic oxo acid + L-glutamate

Gene Name:

tyrB

Locus Tag:

PA3139

Molecular weight:

43.3 kDa

Reactions

General function:

Involved in oxidoreductase activity, acting on a sulfur group of donors, disulfide as acceptor

Specific function:

Could have an important function as a repair enzyme for proteins that have been inactivated by oxidation. Catalyzes the reversible oxidation-reduction of methionine sulfoxide in proteins to methionine

Gene Name:

msrA

Locus Tag:

PA5018

Molecular weight:

23.5 kDa

Reactions

General function:

Involved in peptide-methionine-(S)-S-oxide reductase activity

Specific function:

Peptide-L-methionine + thioredoxin disulfide + H(2)O = peptide-L-methionine (R)-S-oxide + thioredoxin

Gene Name:

msrB

Locus Tag:

PA2827

Molecular weight:

14.8 kDa

Reactions

General function:

Involved in methionine adenosyltransferase activity

Specific function:

Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the enzyme. Is essential for growth

Gene Name:

metK

Locus Tag:

PA0546

Molecular weight:

42.7 kDa

Reactions

General function:

Involved in [formate-C-acetyltransferase]-activating enzyme activity

Specific function:

Activation of anaerobic ribonucleoside-triphosphate reductase under anaerobic conditions by generation of an organic free radical, using S-adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine

Gene Name:

nrdG

Locus Tag:

PA1919

Molecular weight:

25.7 kDa

General function:

Involved in catalytic activity

Specific function:

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism

Gene Name:

bioB

Locus Tag:

PA0500

Molecular weight:

39.1 kDa

Reactions

General function:

Involved in methionine synthase activity

Specific function:

Catalyzes the transfer of a methyl group from methyl- cobalamin to homocysteine, yielding enzyme-bound cob(I)alamin and methionine. Subsequently, remethylates the cofactor using methyltetrahydrofolate

Gene Name:

metH

Locus Tag:

PA1843

Molecular weight:

135.1 kDa

Reactions

General function:

Involved in 5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity

Specific function:

Catalyzes the transfer of a methyl group from 5- methyltetrahydrofolate to homocysteine resulting in methionine formation

Gene Name:

metE

Locus Tag:

PA1927

Molecular weight:

86.2 kDa

Reactions

General function:

Involved in coproporphyrinogen oxidase activity

Specific function:

Anaerobic transformation of coproporphyrinogen-III into protoporphyrinogen-IX

Gene Name:

hemN

Locus Tag:

PA1546

Molecular weight:

52.5 kDa

Reactions

General function:

Involved in coproporphyrinogen oxidase activity

Specific function:

Not Available

Gene Name:

yggW

Locus Tag:

PA0386

Molecular weight:

42.5 kDa

General function:

Involved in catalytic activity

Specific function:

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives. Free octanoate is not a substrate for lipA

Gene Name:

lipA

Locus Tag:

PA2862

Molecular weight:

32.7 kDa

Reactions

General function:

Involved in catalytic activity

Specific function:

Catalyzes the rearrangement of 1-deoxy-D-xylulose 5- phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S

Gene Name:

thiG

Locus Tag:

PA0381

Molecular weight:

28.2 kDa

Reactions

General function:

Involved in thiamine biosynthetic process

Specific function:

Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction

Gene Name:

thiC

Locus Tag:

PA4973

Molecular weight:

69.8 kDa

Reactions

General function:

Involved in electron carrier activity

Specific function:

Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions

Gene Name:

trxA

Locus Tag:

PA5240

Molecular weight:

11.9 kDa

General function:

Not Available

Specific function:

Not Available

Gene Name:

yncA

Locus Tag:

PA4866

Molecular weight:

18.7 kDa

General function:

Translation, ribosomal structure and biogenesis

Specific function:

Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA)

Gene Name:

queA

Locus Tag:

PA3824

Molecular weight:

38.2 kDa

Reactions

General function:

Involved in 4 iron, 4 sulfur cluster binding

Specific function:

Specifically methylates position 2 of adenine 2503 in 23S rRNA

Gene Name:

rlmN

Locus Tag:

PA3806

Molecular weight:

41.7 kDa

Reactions

General function:

tRNA methylthiolation

Specific function:

Catalyzes the methylthiolation of N6-(dimethylallyl)adenosine (i(6)A), leading to the formation of 2-methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine.

Gene Name:

miaB

Locus Tag:

PA3980

Molecular weight:

50 kDa

Reactions

General function:

RNA modification

Specific function:

Catalyzes the methylthiolation of the residue Asp-89 of ribosomal protein S12.

Gene Name:

rimO

Locus Tag:

PA0916

Molecular weight:

49 kDa

Reactions