tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase (PA3980)

Identification
Name: tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase
Synonyms: Not Available
Gene Name: miaB
Enzyme Class:
Biological Properties
General Function: tRNA methylthiolation
Specific Function: Catalyzes the methylthiolation of N6-(dimethylallyl)adenosine (i(6)A), leading to the formation of 2-methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine.
Cellular Location: Not Available
KEGG Pathways: Not Available
KEGG Reactions:
2 Thumb ↔ Thumb+2 Thumb+Thumb
2 S-Adenosylmethionine ↔ S-Adenosylhomocysteine + 2 L-Methionine + 5'-Deoxyadenosine
ReactionCard
SMPDB Reactions: Not Available
PseudoCyc/BioCyc Reactions:
2 Thumb ↔ Thumb+2 Thumb+Thumb
2 S-Adenosylmethionine ↔ S-Adenosylhomocysteine + 2 L-Methionine + 5'-Deoxyadenosine
ReactionCard
6-Dimethylallyladenosine37-tRNAs+PAMDB000289 → PAMDB120072+PAMDB000205+PAMDB000175+PAMDB001583+PAMDB001633
6-Dimethylallyladenosine37-tRNAs + S-Adenosylmethionine → 2-methylthio-N6-dimethylallyladenosine37 in tRNA + S-Adenosylhomocysteine + L-Methionine + 5'-Deoxyadenosine + Hydrogen ion
ReactionCard
PAMDB120230+PAMDB000289 → PAMDB120072+PAMDB000205+PAMDB001633
2-thio-N6-dimethylallyladenosine37 in tRNA + S-Adenosylmethionine → 2-methylthio-N6-dimethylallyladenosine37 in tRNA + S-Adenosylhomocysteine + Hydrogen ion
ReactionCard
6-Dimethylallyladenosine37-tRNAs+PAMDB000289 → PAMDB120230+PAMDB000175+PAMDB001583
6-Dimethylallyladenosine37-tRNAs + S-Adenosylmethionine → 2-thio-N6-dimethylallyladenosine37 in tRNA + L-Methionine + 5'-Deoxyadenosine
ReactionCard
Complex Reactions: Not Available
Transports: Not Available
Metabolites:
PAMDB IDNameView
PAMDB0015835'-DeoxyadenosineMetaboCard
PAMDB000175L-MethionineMetaboCard
PAMDB000205S-AdenosylhomocysteineMetaboCard
PAMDB000289S-AdenosylmethionineMetaboCard
GO Classification:
Function
4 iron, 4 sulfur cluster binding
cytoplasm
iron ion binding
N6-isopentenyladenosine methylthiotransferase activity
tRNA methylation
tRNA methylthiolation
Gene Properties
Locus tag: PA3980
Strand: +
Entrez Gene ID: 878903
Accession: NP_252669.1
GI: 15599175
Sequence start: 4459879
Sequence End: 4461219
Sequence Length: 1340
Gene Sequence: 
>PA3980
ATGGCCAAGAAGCTTTTCATCGAAACCCACGGCTGCCAGATGAACGAGTACGACTCGTCGCGAATGGCGGACCTGCTCGGTGAACACCAAGCCCTCGAGGTGACCGAAAACGCCGCCGAGGCCGACGTCATCCTGCTCAATACCTGCTCGATCCGCGAGAAGGCCCAGGAAAAGGTGTTTTCCAAGCTCGGCATGTGGCGCGAGCTGAAGCAGCAGAATCCCGACCTGGTGATCGGCGTCGGCGGCTGCGTGGCCAGCCAGGAAGGCGCCGCGATCCGTGAGCGCGCGCCCTATGTCGACGTGGTCTTCGGCCCGCAGACCCTGCACCGCCTGCCGGAAATGATCGACGCCGCGCGCAGCACGCGCAAGCCGCAGGTCGACGTGTCCTTCCCGGAAATCGAGAAATTCGACCGCCTGCCGGAACCGCGGGTCGATGGCCCCACCGCCTTCGTCTCGGTGATGGAAGGCTGCAGCAAGTACTGCAGCTTCTGCGTGGTGCCCTACACCCGCGGCGAGGAAGTCAGCCGACCATTCGACGACGTGATCGCCGAAGTGATCCACCTGGCCGAGAACGGCGTCCGCGAAGTCACCCTGCTGGGACAGAACGTCAACGGCTTCCGCGGCCTGACCCACGATGGCCGCCTGGCCGACTTCGCCGAACTGCTGCGGGTGGTCGCCGCCGTCGACGGTATCGAGCGCATCCGCTATACCACCTCGCACCCGCTGGAATTCTCCGACGCGCTGATCCAGGCCCACGCCGAGGTGCCGGAGCTGGTGAAATTCATCCACCTGCCGGTGCAGTCGGGCTCCGACCGCGTGCTGGCGGCGATGAAGCGCAACCATACCGTGCTGGAATACAAGTCGCGTATCCGCAAGCTGAAGGCCGCGGTACCGGACATCTGCATCAGCTCCGACTTCATCGTCGGTTTCCCCGGCGAAACCGAGAAGGACTTCGAGCAGACCATGAAGCTGGTGGAGGACGTCGGCTTCGACTTCTCCTTCTCCTTCATCTACAGCGCGCGGCCCGGCACGCCCGCCGCCGACCTCGCCGACGACCTGCCGGAAGAAGTGAAGAAACAGCGCCTGCAGATCCTCCAGAGCCGCATCCACCAGCAAGGCTACGAGATCAGCCGGCGCATGGTCGGCAGCACCCAGCGGATCCTGGTCACCGACTTCTCGAAGAAGGACCCAGGCATGCTCCAGGGGCGCACCGAGAACAACCGGATCGTCAACTTCCGCTGCGACAATCCACGCCTGATCGGCCAGTTCGCCCAGGTGCACATCGACGACGCGCTGCCGCACTCGCTGCGCGGTACCCTGATCGACAGCACCCTGCATTGA
Protein Properties
Protein Residues: 446
Protein Molecular Weight: 50 kDa
Protein Theoretical pI: 5.78
Hydropathicity (GRAVY score): -0.267
Charge at pH 7 (predicted): -9.09
Protein Sequence: 
>PA3980
MAKKLFIETHGCQMNEYDSSRMADLLGEHQALEVTENAAEADVILLNTCSIREKAQEKVFSKLGMWRELKQQNPDLVIGVGGCVASQEGAAIRERAPYVDVVFGPQTLHRLPEMIDAARSTRKPQVDVSFPEIEKFDRLPEPRVDGPTAFVSVMEGCSKYCSFCVVPYTRGEEVSRPFDDVIAEVIHLAENGVREVTLLGQNVNGFRGLTHDGRLADFAELLRVVAAVDGIERIRYTTSHPLEFSDALIQAHAEVPELVKFIHLPVQSGSDRVLAAMKRNHTVLEYKSRIRKLKAAVPDICISSDFIVGFPGETEKDFEQTMKLVEDVGFDFSFSFIYSARPGTPAADLADDLPEEVKKQRLQILQSRIHQQGYEISRRMVGSTQRILVTDFSKKDPGMLQGRTENNRIVNFRCDNPRLIGQFAQVHIDDALPHSLRGTLIDSTLH
References
External Links:
Resource Link
Genome ID: PA3980
Entrez Gene ID: 878903
NCBI Protein ID: 15599175
General Reference: PaperBLAST - Find papers about PA3980 and its homologs