S-adenosylmethionine:tRNA ribosyltransferase-isomerase (PA3824)

Identification
Name: S-adenosylmethionine:tRNA ribosyltransferase-isomerase
Synonyms:
  • Queuosine biosynthesis protein queA
Gene Name: queA
Enzyme Class:
Biological Properties
General Function: Translation, ribosomal structure and biogenesis
Specific Function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA)
Cellular Location: Cytoplasm (Potential)
KEGG Pathways:
KEGG Reactions: Not Available
SMPDB Reactions:
7-aminomethyl-7-deazaguanosine34 in tRNA+S-adenosyl-L-methionine → Thumb+Thumb+Thumb+Thumb
7-aminomethyl-7-deazaguanosine34 in tRNA + S-adenosyl-L-methionine → Hydrogen ion + L-Methionine + Adenine + epoxyqueuosine
ReactionCard
PseudoCyc/BioCyc Reactions:
7-aminomethyl-7-deazaguanosine34 in tRNA+S-adenosyl-L-methionine → Thumb+Thumb+Thumb+Thumb
7-aminomethyl-7-deazaguanosine34 in tRNA + S-adenosyl-L-methionine → Hydrogen ion + L-Methionine + Adenine + epoxyqueuosine
ReactionCard
tRNA-with-7-aminomethyl-7-deazaguanine+PAMDB000289 → tRNAs-containing-epoxy-quenosine+PAMDB000010+PAMDB000175+PAMDB001633
tRNA-with-7-aminomethyl-7-deazaguanine + S-Adenosylmethionine → tRNAs-containing-epoxy-quenosine + Adenine + L-Methionine + Hydrogen ion
ReactionCard
Complex Reactions: Not Available
Transports: Not Available
Metabolites:
PAMDB IDNameView
PAMDB0036007-Aminomethyl-7-deazaguanosineMetaboCard
PAMDB000010AdenineMetaboCard
PAMDB003576epoxyqueuosineMetaboCard
PAMDB001633Hydrogen ionMetaboCard
PAMDB000175L-MethionineMetaboCard
GO Classification:
Function
catalytic activity
isomerase activity
transferase activity
Process
cellular macromolecule metabolic process
macromolecule metabolic process
metabolic process
ncRNA metabolic process
queuosine biosynthetic process
RNA metabolic process
tRNA metabolic process
tRNA modification
tRNA processing
Gene Properties
Locus tag: PA3824
Strand: -
Entrez Gene ID: 879887
Accession: NP_252513.1
GI: 15599019
Sequence start: 4280520
Sequence End: 4281563
Sequence Length: 1043
Gene Sequence: 
>PA3824
ATGCGTGTCGCCGATTTTCATTTCGACTTGCCCGAGGCCCTGATCGCCCGTCATCCCCTGCCTGAACGCCGCGCAAGCCGCCTGTTGGCGCTGGATGGCCCGACGGGAACCCTCGCCCACAGACAATTCGCCGATCTCCTGGACTACCTGCGTCCGGGCGACCTGATGGTGTTCAACAATACCCGGGTAATCCCGGCGCGCCTGTTCGGCCAGAAGGAATCCGGCGGCAAGCTGGAAGTCCTGGTCGAGCGCGTGCTCGACCAGCACCGGGTGCTTGCCCACATACGCGCCAGCAAGGCGCCCAAGCCCGGCACGCGGATCCTCGTCGAAGGCGGTGGCAGCGCGGAGATGCTGCAACGCCACGACGCCCTGTTCGAACTCGCGTTCGCCGAACCCGTGCTGCCTTTGCTGGAGCGGGTCGGGCACATGCCGTTGCCTCCCTATATCGACCGACCGGACGATGCGGCCGACCGCGAGCGCTACCAGACCGTCTACGCCCAGCGCGCCGGTGCGGTGGCGGCCCCGACCGCCGGCCTGCACTTCGATGAGGCGCTGCTCGAGGCGATCCGCGCGAAGGGGGTGGATACCGCCTTCGTTACCCTGCATGTCGGCGCCGGTACCTTCCAGCCGGTGCGTGTCGAGCGTATCGAGGACCATGTGATGCACCGCGAATGGCTGGAGGTCGGCCAGGACGTGGTGGACGCGGTTTCCGTCTGCCGGGCCCGCGGCGGCCGGGTAGTCGCCGTCGGCACCACCAGCGTGCGCTCGCTGGAAAGCGCGGCACGCGACGGTGAGCTGAAGCCCTTCAGCGGCGACACCGACATCTTCATCTACCCGGGCCGGCCGTTCCATGTGGTCGATGCCCTGGTCACCAACTTCCACCTGCCCGAATCCACCCTGCTGATGCTGGTCTCGGCCTTCGCCGGCTACCCGGAGACCATGGCCGCCTACGCCGCGGCGGTGGCGCAGGGCTATCGCTTCTTCAGTTACGGTGATGCCATGTTCATCACCCGCAATCCCGCGCCCCGCGGACCCGAGGACTGA
Protein Properties
Protein Residues: 347
Protein Molecular Weight: 38.2 kDa
Protein Theoretical pI: 6.45
Hydropathicity (GRAVY score): -0.09
Charge at pH 7 (predicted): -3.89
Protein Sequence: 
>PA3824
MRVADFHFDLPEALIARHPLPERRASRLLALDGPTGTLAHRQFADLLDYLRPGDLMVFNNTRVIPARLFGQKESGGKLEVLVERVLDQHRVLAHIRASKAPKPGTRILVEGGGSAEMLQRHDALFELAFAEPVLPLLERVGHMPLPPYIDRPDDAADRERYQTVYAQRAGAVAAPTAGLHFDEALLEAIRAKGVDTAFVTLHVGAGTFQPVRVERIEDHVMHREWLEVGQDVVDAVSVCRARGGRVVAVGTTSVRSLESAARDGELKPFSGDTDIFIYPGRPFHVVDALVTNFHLPESTLLMLVSAFAGYPETMAAYAAAVAQGYRFFSYGDAMFITRNPAPRGPED
References
External Links:
Resource Link
Genome ID: PA3824
Entrez Gene ID: 879887
NCBI Protein ID: 15599019
General Reference: PaperBLAST - Find papers about PA3824 and its homologs