5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (PA1927)

Identification
Name: 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase
Synonyms:
  • Cobalamin-independent methionine synthase
  • Methionine synthase, vitamin-B12 independent isozyme
Gene Name: metE
Enzyme Class:
Biological Properties
General Function: Involved in 5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity
Specific Function: Catalyzes the transfer of a methyl group from 5- methyltetrahydrofolate to homocysteine resulting in methionine formation
Cellular Location: Not Available
KEGG Pathways:
KEGG Reactions:
Thumb+Thumb ↔ Thumb+Thumb+Thumb
5-Methyltetrahydrofolic acid + L-Homocysteine ↔ Hydrogen ion + L-Methionine + Tetrahydrofolic acid
ReactionCard
Thumb+Thumb ↔ Thumb+Thumb
5-Methyltetrahydropteroyltri-L-glutamic acid + L-Homocysteine ↔ Tetrahydropteroyltri-L-glutamic acid + L-Methionine
ReactionCard
SMPDB Reactions:
Homocysteine+Thumb+Thumb → Thumb+Thumb
Homocysteine + N5-methyl--tetrahydropteroyl tri-L-glutamate + Homocysteine → L-Methionine + tetrahydropteroyltri-L-glutamate
ReactionCard
PseudoCyc/BioCyc Reactions:
Thumb+Thumb ↔ Thumb+Thumb+Thumb
5-Methyltetrahydrofolic acid + L-Homocysteine ↔ Hydrogen ion + L-Methionine + Tetrahydrofolic acid
ReactionCard
Thumb+Thumb → Thumb+tetrahydropteroyl tri-L-glutamate
L-Homocysteine + 5-Methyltetrahydropteroyltri-L-glutamic acid → L-Methionine + tetrahydropteroyl tri-L-glutamate
ReactionCard
Complex Reactions:
Thumb+Thumb → Thumb+Thumb
5-methyltetrahydropteroyltri-L-glutamate + L-Homocysteine → tetrahydropteroyltri-L-glutamate + L-Methionine
ReactionCard
Transports: Not Available
Metabolites:
PAMDB IDNameView
PAMDB006316 N5-methyl--tetrahydropteroyl tri-L-glutamateMetaboCard
PAMDB0003685-Methyltetrahydrofolic acidMetaboCard
PAMDB0035445-methyltetrahydropteroyltri-L-glutamateMetaboCard
PAMDB0002455-Methyltetrahydropteroyltri-L-glutamic acidMetaboCard
PAMDB006170HomocysteineMetaboCard
PAMDB001633Hydrogen ionMetaboCard
PAMDB000067L-HomocysteineMetaboCard
PAMDB000175L-MethionineMetaboCard
PAMDB001428SelenohomocysteineMetaboCard
PAMDB000424Tetrahydrofolic acidMetaboCard
PAMDB003550tetrahydropteroyltri-L-glutamateMetaboCard
PAMDB000283Tetrahydropteroyltri-L-glutamic acidMetaboCard
GO Classification:
Function
5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity
binding
catalytic activity
cation binding
ion binding
metal ion binding
methyltransferase activity
S-methyltransferase activity
transferase activity
transferase activity, transferring one-carbon groups
transition metal ion binding
zinc ion binding
Process
cellular amino acid and derivative metabolic process
cellular amino acid biosynthetic process
cellular amino acid metabolic process
cellular metabolic process
metabolic process
methionine biosynthetic process
sulfur amino acid biosynthetic process
sulfur amino acid metabolic process
Gene Properties
Locus tag: PA1927
Strand: +
Entrez Gene ID: 877662
Accession: NP_250617.1
GI: 15597123
Sequence start: 2106580
Sequence End: 2108880
Sequence Length: 2300
Gene Sequence: 
>PA1927
ATGGCCCTGGCCCACACTCTCGGCTTCCCACGCATCGGACGCGATCGCGAACTGAAAAAAGCCCAGGAAGCGTTCTGGAAGGGCGAGCTGGACGAGGCCGGCCTGCGGGCCGTCGGTCGGCAGCTGCGCGCTGCCCACTGGCAGGTACAGAAGGATGCCGGCATCCAGTTGCTGCCGGTCGGCGATTTCGCCTGGTATGACCAGGTGCTGACCCATTCGCTGACCTTCGGCGTGATCCCCGAGCGCTTCCGGGCGCACGGCGAGGCCGGGCCGACCCTGCATACCCTGTTCGGCATGGCCCGTGGCGTCAGCGACGACAGTTGTTGCGGCGGTGCCCATGCCCAGGAAATGACCAAGTGGTTCGACACCAACTATCACTACCTGGTCCCCGAGTTCAGCGCCGATCAGCAGTTCCAGCTGAGCTGGGAACAACTGTTCGAGGAGGTCGACGAGGCCAGGGCCCTCGGCCACCAGGTGAAGCCGGTGCTGATCGGTCCGCTGACCTACCTGTGGCTGGGCAAGGCCAAGGGCGCCGAGTTCGACAGGCTCGACCTGCTGGACCGCCTGCTGCCGCTCTACGGCCAGATCTTCCGTCGTCTCGCCGGCCAGGGCGTGGAGTGGGTGCAGATCGACGAGCCGATCCTGGTCCTCGACCTGCCGCAGGCATGGAAGAATGCCTTCGAGCGCGCCTACAACCTGCTACAGAGCGAGCCGCTGAAGAAGCTGGTCGCCACCTACTTCGGCGGCCTGGAAGACAACCTGGGCCTGGCCGCCAACCTCCCGGTGGACGGCCTGCACATCGACCTGGTGCGAGCGCCGGAACAGTATCCGAGCATCCTCGACCGCCTGCCGGCCTACAAGGTCGTCTCGCTGGGCCTGGTCAACGGGCGCAACGTCTGGCGCTGCGACCTGGAGAAAGCCCTGGAGGTGGTGCGCCATGCCCGGGAGCGCCTGGGCGAGCGGCTGTGGGTCGCGCCGTCCTGCTCGCTGCTGCACAGCCCGGTGGACCTGGAACGCGAAGACGGGCTGGACGCCGAGCTGAAGAGCTGGCTGGCCTTCGCCGTGCAGAAGTGCCGCGAAGTCGCGGTGCTGGCCCGTGCCGCCACTGAGCCGGAAGCCGCCGAGGTGCTTGCCGCCCTGGAAGAGAGCCGTGCGGTCCAGGCCAGCCGCGCTTCCTCGCCGCGAATCCACAAGCCGGCCGTGCAGGCGCGCCTGGCCGCGATCAAGGCCAGCGACGCCCAGCGTCGCTCGCCGTTCGCCGAACGCATCGCCAGGCAGCGTGCCGGGCTCGACCTGCCGGCGTTCCCGACCACTACCATCGGCTCTTTCCCGCAGACCTCGTCGATCCGCCTGGCCCGCCAGTCGTTCAAGCAGGGCAAGCTGAGCGAGGCCGAGTACATCGAGGCGATGCACAGCGAGATCCGCCATGCGGTGCAGATCCAGGAACAACTGGGGCTGGACGTGCTGGTACATGGCGAAGCCGAGCGCAACGACATGGTCGAGTACTTCGCCGAGCAACTGGACGGCTACGTCTTCACCCGCTTCGGCTGGGTGCAGAGCTACGGCTCGCGCTGCGTGAAGCCGGCGGTGATCTATGGCGACCTGAGCCGTCCGCGGGCCATGACCGTGGAGTGGATCAGGTACGCCCAGAGCCTTACCGACAAGGTGATGAAGGGCATGCTGACCGGCCCGGTGACCATGCTGATGTGGTCCTTCCCGCGCGAGGACGTGTCTCGCGAGGTGCAGGCGCGCCAGTTGGCCCTGGCGATCCGCGACGAAGTGGTCGACCTGGAGGCCGCCGGCATCAGGATCGTGCAGATCGACGAGGCGGCTTTCCGCGAAGGCCTGCCGCTGCGCCGCAACGCCTGGCCGCACTATCTGGAGTGGGCCACCGAGGCCTTCCGCCTGTGCGCCTCCGGAGTCCGCGACGAAACCCAGATCCATACGCACATGTGCTACAGCGAGTTCAACGATGTGATCGAGTCCATCGCCGCCATGGACGCCGACGTGATCACCATCGAGACCTCGCGTTCGGACATGGAGCTGCTGGAGGCTTTCGAGCAGTTCGACTACCCGAACGAGATCGGTCCGGGCGTCTACGACATCCACTCGCCACGCGTGCCGAGCCGGGAAGAGATCGTCGCGTTGCTGCGCAAGGCGGCCCGGCGGATACCCGCCGAGCGGCTCTGGGTCAACCCGGACTGCGGCCTGAAGACCCGCGCCTGGCCGGAAACCGAGGCAGCGCTGGTGAACATGGTCGCCGCGGCCCGCGAACTGCGTGGCGACCTCGCCCGCGGTTGA
Protein Properties
Protein Residues: 766
Protein Molecular Weight: 86.2 kDa
Protein Theoretical pI: 5.67
Hydropathicity (GRAVY score): -0.227
Charge at pH 7 (predicted): -14.64
Protein Sequence: 
>PA1927
MALAHTLGFPRIGRDRELKKAQEAFWKGELDEAGLRAVGRQLRAAHWQVQKDAGIQLLPVGDFAWYDQVLTHSLTFGVIPERFRAHGEAGPTLHTLFGMARGVSDDSCCGGAHAQEMTKWFDTNYHYLVPEFSADQQFQLSWEQLFEEVDEARALGHQVKPVLIGPLTYLWLGKAKGAEFDRLDLLDRLLPLYGQIFRRLAGQGVEWVQIDEPILVLDLPQAWKNAFERAYNLLQSEPLKKLVATYFGGLEDNLGLAANLPVDGLHIDLVRAPEQYPSILDRLPAYKVVSLGLVNGRNVWRCDLEKALEVVRHARERLGERLWVAPSCSLLHSPVDLEREDGLDAELKSWLAFAVQKCREVAVLARAATEPEAAEVLAALEESRAVQASRASSPRIHKPAVQARLAAIKASDAQRRSPFAERIARQRAGLDLPAFPTTTIGSFPQTSSIRLARQSFKQGKLSEAEYIEAMHSEIRHAVQIQEQLGLDVLVHGEAERNDMVEYFAEQLDGYVFTRFGWVQSYGSRCVKPAVIYGDLSRPRAMTVEWIRYAQSLTDKVMKGMLTGPVTMLMWSFPREDVSREVQARQLALAIRDEVVDLEAAGIRIVQIDEAAFREGLPLRRNAWPHYLEWATEAFRLCASGVRDETQIHTHMCYSEFNDVIESIAAMDADVITIETSRSDMELLEAFEQFDYPNEIGPGVYDIHSPRVPSREEIVALLRKAARRIPAERLWVNPDCGLKTRAWPETEAALVNMVAAARELRGDLARG
References
External Links:
Resource Link
Genome ID: PA1927
Entrez Gene ID: 877662
NCBI Protein ID: 15597123
General Reference: PaperBLAST - Find papers about PA1927 and its homologs