Biotin synthase (PA0500)

Identification
Name: Biotin synthase
Synonyms: Not Available
Gene Name: bioB
Enzyme Class:
Biological Properties
General Function: Involved in catalytic activity
Specific Function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism
Cellular Location: Not Available
KEGG Pathways:
KEGG Reactions:
Thumb+Sulfur donor+2 Thumb+2 e-+2 Thumb ↔ Thumb+2 Thumb+2 Thumb
Dethiobiotin + Sulfur donor + 2 S-Adenosylmethionine + 2 e- + 2 Hydrogen ion ↔ Biotin + 2 L-Methionine + 2 5'-Deoxyadenosine
ReactionCard
SMPDB Reactions:
Thumb+2 S-adenosyl-L-methionine+2 Thumb+Thumb → Thumb+2 Thumb+2 Thumb
Dethiobiotin + 2 S-adenosyl-L-methionine + 2 Hydrogen ion + a sulfurated [sulfur carrier] → Biotin + 2 L-Methionine + 2 5'-Deoxyadenosine
ReactionCard
PseudoCyc/BioCyc Reactions:
[2Fe-2S] iron-sulfur cluster+Thumb+Thumb → [2Fe-1S] desulfurated iron-sulfur cluster+Thumb+Thumb+Thumb+Thumb
[2Fe-2S] iron-sulfur cluster + S-Adenosylmethionine + Dethiobiotin → [2Fe-1S] desulfurated iron-sulfur cluster + Biotin + 5'-Deoxyadenosine + Hydrogen ion + L-Methionine
ReactionCard
Thumb+Sulfur donor+2 Thumb+2 e-+2 Thumb ↔ Thumb+2 Thumb+2 Thumb
Dethiobiotin + Sulfur donor + 2 S-Adenosylmethionine + 2 e- + 2 Hydrogen ion ↔ Biotin + 2 L-Methionine + 2 5'-Deoxyadenosine
ReactionCard
Thumb+2 S-adenosyl-L-methionine+2 Thumb+Thumb → Thumb+2 Thumb+2 Thumb
Dethiobiotin + 2 S-adenosyl-L-methionine + 2 Hydrogen ion + a sulfurated [sulfur carrier] → Biotin + 2 L-Methionine + 2 5'-Deoxyadenosine
ReactionCard
Thumb
a sulfurated [sulfur carrier] + dethiobiotin + 2 S-adenosyl-L-methionine + 2 a reduced [2Fe-2S] ferredoxin → an unsulfurated [sulfur carrier] + biotin + 2 5'-deoxyadenosine + 2 L-methionine + 2 an oxidized [2Fe-2S] ferredoxin
ReactionCard
Complex Reactions:
[2Fe-2S] iron-sulfur cluster+Thumb+Thumb → [2Fe-1S] desulfurated iron-sulfur cluster+Thumb+Thumb+Thumb+Thumb
[2Fe-2S] iron-sulfur cluster + S-Adenosylmethionine + Dethiobiotin → [2Fe-1S] desulfurated iron-sulfur cluster + Biotin + 5'-Deoxyadenosine + Hydrogen ion + L-Methionine
ReactionCard
Transports: Not Available
Metabolites:
PAMDB IDNameView
PAMDB0015835'-DeoxyadenosineMetaboCard
PAMDB006303a sulfurated [sulfur carrier]MetaboCard
PAMDB000008BiotinMetaboCard
PAMDB000517DethiobiotinMetaboCard
PAMDB001633Hydrogen ionMetaboCard
PAMDB000486Hydrogen sulfideMetaboCard
PAMDB000175L-MethionineMetaboCard
PAMDB000289S-AdenosylmethionineMetaboCard
GO Classification:
Function
binding
biotin synthase activity
catalytic activity
iron-sulfur cluster binding
metal cluster binding
sulfurtransferase activity
transferase activity
transferase activity, transferring sulfur-containing groups
Process
biotin biosynthetic process
biotin metabolic process
cellular metabolic process
cofactor metabolic process
metabolic process
small molecule metabolic process
vitamin metabolic process
water-soluble vitamin metabolic process
Gene Properties
Locus tag: PA0500
Strand: +
Entrez Gene ID: 877668
Accession: NP_249191.1
GI: 15595697
Sequence start: 559644
Sequence End: 560702
Sequence Length: 1058
Gene Sequence: 
>PA0500
ATGAGTGCAACCGCTTCCGTCGCCACCCGTCACGACTGGTCCCTCGCCGAAGTCCGTGCCCTGTTCGAGCAACCGTTCAACGACCTGCTGTTCCAGGCGCAGACGGTGCATCGCGCGCACTTCGACCCGAACCGCGTGCAGGTCTCGACGCTGCTGTCGATCAAGACCGGCGCCTGCCCCGAGGACTGCAAGTACTGCCCGCAGTCCGGCCACTACAACACCGGCCTGGACAAGGAGAAGCTGATGGAGGTGCAGAAGGTCCTCGAGGCGGCGGCCGAGGCCAAGGCCATCGGTTCGACCCGCTTCTGCATGGGCGCTGCCTGGAAGCACCCGTCGGCCAAGGACATGCCCTACGTCCTGGAGATGGTCAAGGGCGTGAAGAAGCTCGGCCTGGAGACCTGCATGACCCTCGGTCGCCTGACCCAGGAACAGACCCAGGCGCTGGCGGACGCCGGCCTCGACTACTACAACCACAACCTGGATACCTCGCCGGAGTTCTACGGCAACATCATCACCACCCGCACCTACAGCGAGCGCCTGCAGACCCTGGCCTACGTGCGCGAGGCGGGGATGAAGATCTGCTCCGGCGGCATCCTCGGCATGGGCGAGTCGGTGGACGACCGCGCCGGCCTGCTGATCCAACTGGCGAACCTGCCGGAGCACCCGGAGTCGGTGCCGATCAACATGCTGGTGAAGGTCAAGGGCACGCCCCTGGCGGAAGAGAAGGACGTCGATCCCTTCGACTTCATCCGCACCCTGGCGGTGGCCCGGATCATGATGCCGAAGTCCCACGTGCGCCTGTCCGCCGGCCGCGAGCAGATGAACGAGCAGATGCAGGCGCTGGCCTTCATGGCCGGGGCCAACTCGATCTTCTACGGCGAAAAGCTGCTGACCACGAAGAACCCGCAGGCCGAAAAGGACATGCAGTTGTTCGCCCGTCTCGGCATCAAGCCGGAAGAGCGCGAAGAGCACGCCGACGAAGTGCACCAGGCCGCCATCGAGCAGGCGTTGGTGGAACAACGCGAATCGAAGCTGTTCTATAACGCCGCTTCCGCCTGA
Protein Properties
Protein Residues: 352
Protein Molecular Weight: 39.1 kDa
Protein Theoretical pI: 6.01
Hydropathicity (GRAVY score): -0.315
Charge at pH 7 (predicted): -5.77
Protein Sequence: 
>PA0500
MSATASVATRHDWSLAEVRALFEQPFNDLLFQAQTVHRAHFDPNRVQVSTLLSIKTGACPEDCKYCPQSGHYNTGLDKEKLMEVQKVLEAAAEAKAIGSTRFCMGAAWKHPSAKDMPYVLEMVKGVKKLGLETCMTLGRLTQEQTQALADAGLDYYNHNLDTSPEFYGNIITTRTYSERLQTLAYVREAGMKICSGGILGMGESVDDRAGLLIQLANLPEHPESVPINMLVKVKGTPLAEEKDVDPFDFIRTLAVARIMMPKSHVRLSAGREQMNEQMQALAFMAGANSIFYGEKLLTTKNPQAEKDMQLFARLGIKPEEREEHADEVHQAAIEQALVEQRESKLFYNAASA
References
External Links:
Resource Link
Genome ID: PA0500
Entrez Gene ID: 877668
NCBI Protein ID: 15595697
General Reference: PaperBLAST - Find papers about PA0500 and its homologs