Reaction Details
Reaction: Thumb
a sulfurated [sulfur carrier] + dethiobiotin + 2 S-adenosyl-L-methionine + 2 a reduced [2Fe-2S] ferredoxin → an unsulfurated [sulfur carrier] + biotin + 2 5'-deoxyadenosine + 2 L-methionine + 2 an oxidized [2Fe-2S] ferredoxin
Enzymes:
External Links:
ResourceLink
PseudoCyc Reaction ID:2.8.1.6-RXN
References:
  • Berkovitch F, Nicolet Y, Wan JT, Jarrett JT, Drennan CL (2004). "Crystal structure of biotin synthase, an S-adenosylmethionine-dependent radical enzyme." Science 303(5654);76-9. PMID: 14704425
  • Lotierzo M, Tse Sum Bui B, Florentin D, Escalettes F, Marquet A (2005). "Biotin synthase mechanism: an overview." Biochem Soc Trans 33(Pt 4);820-3. PMID: 16042606
  • Reyda MR, Fugate CJ, Jarrett JT (2009). "A complex between biotin synthase and the iron-sulfur cluster assembly chaperone HscA that enhances in vivo cluster assembly." Biochemistry 48(45);10782-92. PMID: 19821612
  • Shiuan D, Campbell A (1988). "Transcriptional regulation and gene arrangement of Escherichia coli, Citrobacter freundii and Salmonella typhimurium biotin operons." Gene 67(2);203-11. PMID: 2971595
  • Taylor AM, Farrar CE, Jarrett JT (2008). "9-Mercaptodethiobiotin is formed as a competent catalytic intermediate by Escherichia coli biotin synthase." Biochemistry 47(35);9309-17. PMID: 18690713
  • Ugulava NB, Gibney BR, Jarrett JT (2001). "Biotin synthase contains two distinct iron-sulfur cluster binding sites: chemical and spectroelectrochemical analysis of iron-sulfur cluster interconversions." Biochemistry 40(28);8343-51. PMID: 11444981
  • Zhang S, Sanyal I, Bulboaca GH, Rich A, Flint DH (1994). "The gene for biotin synthase from Saccharomyces cerevisiae: cloning, sequencing, and complementation of Escherichia coli strains lacking biotin synthase." Arch Biochem Biophys 309(1);29-35. PMID: 8117110