Thiazole synthase (PA0381)

Identification
Name: Thiazole synthase
Synonyms: Not Available
Gene Name: thiG
Enzyme Class:
Biological Properties
General Function: Involved in catalytic activity
Specific Function: Catalyzes the rearrangement of 1-deoxy-D-xylulose 5- phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S
Cellular Location: Cytoplasm
KEGG Pathways:
KEGG Reactions:
C15815+Thumb+Iminoglycine ↔ Thumb
C15815 + L-Tyrosine + Iminoglycine ↔ 4-Methyl-5-(2-phosphoethyl)-thiazole
ReactionCard
Thumb+Thumb ↔ Thumb+2 Thumb
1-Deoxy-D-xylulose 5-phosphate + 2-iminoacetate ↔ 2-[(2R,5Z)-2-Carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate + 2 Water
ReactionCard
SMPDB Reactions:
Thumb+Thumb+a thiocarboxy-[ThiS-Protein] → Thumb+a ThiS sulfur-carrier protein+2 Thumb
1-Deoxy-D-xylulose 5-phosphate + Dehydroglycine + a thiocarboxy-[ThiS-Protein] → 2-((2R,5Z)-2-Carboxy-4-methylthiazol-5(2H)-ylidene)ethyl phosphate + a ThiS sulfur-carrier protein + 2 Water
ReactionCard
PseudoCyc/BioCyc Reactions:
Thumb+Thumb+Thumb → Thumb+Thumb+Thumb+Thumb+Thumb+Thumb
S-Adenosylmethionine + NADPH + L-Tyrosine → p-Cresol + 5'-Deoxyadenosine + Dehydroglycine + Hydrogen ion + L-Methionine + NADP
ReactionCard
C15815+Thumb+Iminoglycine ↔ Thumb
C15815 + L-Tyrosine + Iminoglycine ↔ 4-Methyl-5-(2-phosphoethyl)-thiazole
ReactionCard
Thumb+Thumb+thiocarboxy-adenylate-[sulfur-carrier protein ThiS] → Thumb+[sulfur-carrier protein ThiS]+2 Thumb
1-Deoxy-D-xylulose 5-phosphate + 2-iminoacetate + thiocarboxy-adenylate-[sulfur-carrier protein ThiS] → 2-((2R,5Z)-2-Carboxy-4-methylthiazol-5(2H)-ylidene)ethyl phosphate + [sulfur-carrier protein ThiS] + 2 Water
ReactionCard
Thumb+Thumb ↔ Thumb+2 Thumb
1-Deoxy-D-xylulose 5-phosphate + 2-iminoacetate ↔ 2-[(2R,5Z)-2-Carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate + 2 Water
ReactionCard
Complex Reactions:
Thumb+Thumb+Thumb → Thumb+Thumb+Thumb+Thumb+Thumb+Thumb
S-Adenosylmethionine + NADPH + L-Tyrosine → p-Cresol + 5'-Deoxyadenosine + Dehydroglycine + Hydrogen ion + L-Methionine + NADP
ReactionCard
Thumb+Thumb+thiocarboxy-adenylate-[sulfur-carrier protein ThiS] → Thumb+[sulfur-carrier protein ThiS]+2 Thumb
1-Deoxy-D-xylulose 5-phosphate + 2-iminoacetate + thiocarboxy-adenylate-[sulfur-carrier protein ThiS] → 2-((2R,5Z)-2-Carboxy-4-methylthiazol-5(2H)-ylidene)ethyl phosphate + [sulfur-carrier protein ThiS] + 2 Water
ReactionCard
Transports: Not Available
Metabolites:
PAMDB IDNameView
PAMDB0003011-Deoxy-D-xylulose 5-phosphateMetaboCard
PAMDB0034432-((2R,5Z)-2-Carboxy-4-methylthiazol-5(2H)-ylidene)ethyl phosphateMetaboCard
PAMDB0035452-iminoacetateMetaboCard
PAMDB0045432-[(2R,5Z)-2-Carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphateMetaboCard
PAMDB0009424-Methyl-5-(2-phosphoethyl)-thiazoleMetaboCard
PAMDB0015835'-DeoxyadenosineMetaboCard
PAMDB001730DehydroglycineMetaboCard
PAMDB001633Hydrogen ionMetaboCard
PAMDB000175L-MethionineMetaboCard
PAMDB000060L-TyrosineMetaboCard
PAMDB000089NADPMetaboCard
PAMDB000615NADPHMetaboCard
PAMDB001747p-CresolMetaboCard
PAMDB000289S-AdenosylmethionineMetaboCard
PAMDB000142WaterMetaboCard
GO Classification:
Function
catalytic activity
Process
cellular aromatic compound metabolic process
cellular metabolic process
metabolic process
thiamin and derivative metabolic process
thiamin biosynthetic process
thiamin metabolic process
Gene Properties
Locus tag: PA0381
Strand: +
Entrez Gene ID: 879802
Accession: NP_249072.1
GI: 15595578
Sequence start: 423719
Sequence End: 424516
Sequence Length: 797
Gene Sequence: 
>PA0381
ATGAGCCAAGCATCCAGTACCGATACCCCCTTCGTCATCGCCGGCCGTACCTATGGCTCGCGCCTGCTGGTGGGGACCGGCAAGTACAAGGACCTCGACGAGACCCGCCGGGCCATCGAGGCCTCCGGAGCGGAGATCGTCACGGTGGCGGTGCGGCGCACCAACATCGGGCAGAATCCCGACGAGCCGAACCTGCTGGATGTGATCCCGCCGGATCGCTACACCATCCTGCCGAACACCGCCGGCTGCTATGACGCGGTCGAGGCCGTGCGCACCTGCCGCCTGGCCCGCGAGCTGCTGGACGGGCACAACCTGGTCAAGCTGGAAGTCCTCGCCGACCAGAAGACCCTGTTCCCCAACGTCGTCGAGACCCTCAAGGCCGCCGAGCAACTGGTGAAGGACGGCTTCGACGTGATGGTGTACACCAGCGACGACCCGATCATCGCCCGCCAACTGGCCGAGATCGGCTGCATCGCGGTGATGCCGCTGGCCGGCCTGATCGGCTCCGGCCTGGGCATCTGCAACCCCTACAACCTGCGCATCATCCTCGAGGAAGCCAAGGTCCCGGTGCTGGTAGACGCCGGCGTGGGCACCGCTTCCGATGCAGCGATCGCCATGGAGCTGGGTTGCGAGGCGGTGCTGATGAACACCGCCATCGCCCATGCCAAGGACCCGGTGATGATGGCCGAGGCCATGAAGCACGCGATCGTCGCCGGTCGTCTCGCCTACCTGGCCGGACGCATGCCGCGCAAGCTGTATGCCAGCGCCTCGTCGCCGCTGGACGGCCTGATCGACTGA
Protein Properties
Protein Residues: 265
Protein Molecular Weight: 28.2 kDa
Protein Theoretical pI: 4.75
Hydropathicity (GRAVY score): 0.182
Charge at pH 7 (predicted): -7.43
Protein Sequence: 
>PA0381
MSQASSTDTPFVIAGRTYGSRLLVGTGKYKDLDETRRAIEASGAEIVTVAVRRTNIGQNPDEPNLLDVIPPDRYTILPNTAGCYDAVEAVRTCRLARELLDGHNLVKLEVLADQKTLFPNVVETLKAAEQLVKDGFDVMVYTSDDPIIARQLAEIGCIAVMPLAGLIGSGLGICNPYNLRIILEEAKVPVLVDAGVGTASDAAIAMELGCEAVLMNTAIAHAKDPVMMAEAMKHAIVAGRLAYLAGRMPRKLYASASSPLDGLID
References
External Links:
Resource Link
Genome ID: PA0381
Entrez Gene ID: 879802
NCBI Protein ID: 15595578
General Reference: PaperBLAST - Find papers about PA0381 and its homologs