Phosphomethylpyrimidine synthase (PA4973)

Identification
Name: Phosphomethylpyrimidine synthase
Synonyms:
  • Hydroxymethylpyrimidine phosphate synthase
  • HMP-P synthase
  • HMP-phosphate synthase
  • HMPP synthase
  • Thiamine biosynthesis protein thiC
Gene Name: thiC
Enzyme Class:
Biological Properties
General Function: Involved in thiamine biosynthetic process
Specific Function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction
Cellular Location: Not Available
KEGG Pathways:
KEGG Reactions:
Thumb+Thumb ↔ Thumb+Thumb+Thumb+Thumb+Thumb+Thumb
4-Amino-5-hydroxymethyl-2-methylpyrimidine + S-Adenosylmethionine ↔ 5-Aminoimidazole ribonucleotide + 4-Amino-2-methyl-5-phosphomethylpyrimidine + 5'-Deoxyadenosine + L-Methionine + Formic acid + CO
ReactionCard
SMPDB Reactions:
Thumb+S-adenosyl-L-methionine → 3 Thumb+Thumb+Thumb+Thumb+Thumb+4-amino-2-methyl-5-phosphomethylpyrimidine
5-Aminoimidazole ribonucleotide + S-adenosyl-L-methionine → 3 Hydrogen ion + CO + Formic acid + L-Methionine + 5'-Deoxyadenosine + 4-amino-2-methyl-5-phosphomethylpyrimidine
ReactionCard
PseudoCyc/BioCyc Reactions:
Thumb+Thumb ? Thumb+Thumb+Thumb+Thumb+carbon monoxide+Thumb
5-Aminoimidazole ribonucleotide + S-Adenosylmethionine ? 4-Amino-2-methyl-5-phosphomethylpyrimidine + 5'-Deoxyadenosine + L-Methionine + Formic acid + carbon monoxide + Hydrogen ion
ReactionCard
Complex Reactions:
Thumb+Thumb+Thumb → Thumb+2 Thumb+3 Thumb+Thumb
5-Aminoimidazole ribonucleotide + Water + NAD → 4-Amino-2-methyl-5-phosphomethylpyrimidine + 2 Formic acid + 3 Hydrogen ion + NADH
ReactionCard
Transports: Not Available
Metabolites:
PAMDB IDNameView
PAMDB0009324-Amino-2-methyl-5-phosphomethylpyrimidineMetaboCard
PAMDB0009344-Amino-5-hydroxymethyl-2-methylpyrimidineMetaboCard
PAMDB0015835'-DeoxyadenosineMetaboCard
PAMDB0003065-Aminoimidazole ribonucleotideMetaboCard
PAMDB003394COMetaboCard
PAMDB000053Formic acidMetaboCard
PAMDB001633Hydrogen ionMetaboCard
PAMDB000175L-MethionineMetaboCard
PAMDB000197NADMetaboCard
PAMDB000398NADHMetaboCard
PAMDB000289S-AdenosylmethionineMetaboCard
PAMDB000142WaterMetaboCard
GO Classification:
Process
cellular aromatic compound metabolic process
cellular metabolic process
metabolic process
thiamin and derivative metabolic process
thiamin biosynthetic process
thiamin metabolic process
Gene Properties
Locus tag: PA4973
Strand: -
Entrez Gene ID: 879702
Accession: NP_253660.1
GI: 15600166
Sequence start: 5581762
Sequence End: 5583645
Sequence Length: 1883
Gene Sequence: 
>PA4973
ATGAGCGCAACGCAGAAGAACAACATCACCCGCCTTGAGCAGCTCGACCGCCAGTCGACGCAGCCTTTCCCGAACTCGCGCAAGGTCTACCTGACCGGCTCGCGCCCGGACATCCGCGTCCCGGTGCGGGAAATCTCCCTCGCCGACACCCCCACCGCCTTCGGCGGCGAAAAAAACCCGCCGGTGTTCGTCTACGACACCTCCGGCCCGTATACCGACCCTGAGGTCCGCATCGACCTGCGCAAGGGCCTGCCTGACGTGCGTTCGCGCTGGATCGACGAGCGCGGCGACACCGAGATCCTCCCCGGCCTGACCTCCGAGTTCGGCCAGGCCCGCCTGGCCGACGCCAGCCTCGACGCCCTGCGCTTCGCCCACGTGCGCACGCCGCGCCGGGCCAAGCCGGGCGCCAACGTCTCGCAGATGCACTACGCGAAGAAAGGCATCATCACCCCGGAGATGGAATACATTGCCATCCGCGAGAACATGAAGCTGCAGGAAGCCCGGGCCGCCGGCCTGCTCGACCAGCAGCACCCGGGGCACAGCTTCGGTGCCAACATCCCGAAGGAAATCACCCCCGAGTTCGTCCGCGAGGAAGTCGCCCGCGGCCGCGCGATCATTCCGGCGAACATCAACCACACCGAGCTTGAGCCGATGATCATCGGCCGCAACTTCCTGGTGAAGATCAACGGCAACATCGGCAACAGCGCGCTGGGCTCCTCGATCGAGGAAGAAGTGGAGAAGCTCACCTGGGGTATCCGCTGGGGCGCCGACACGGTGATGGACCTGTCCACCGGCAAGCACATCCATGAAACCCGCGAGTGGATCCTGCGCAACAGCCCGGTACCGATCGGCACCGTACCGATCTACCAGGCGCTGGAGAAGGTCAACGGCGTCGCCGAGGACCTGACCTGGGAAATCTTCCGCGACACCCTGATCGAACAGGCCGAGCAGGGCGTCGACTACTTCACCATCCACGCCGGCGTGCTGCTGCGCTATGTGCCGCTGACCGCCAAGCGGGTCACCGGCATCGTTTCCCGCGGCGGCTCGATCATGGCCAAGTGGTGCCTGGCGCACCACCAGGAGAACTTCCTCTACACGCATTTCGAAGAGATCTGCGAAATCATGAAGGCCTACGACGTCAGCTTCTCGCTGGGCGATGGCCTGCGTCCGGGCTCGGTGGCCGACGCCAACGACGCCGCCCAGTTCGGCGAGCTGGAAACCCTCGGCGAGCTGACCAAGATCGCCTGGAAGCACGATGTGCAGGTGATGATCGAAGGTCCCGGCCACGTGCCGATGCAATTGATCAAGGAAAACATGGACAAGCAGCTGGAGTGCTGTGACGAGGCGCCGTTCTACACCCTCGGCCCGCTGACCACCGACATCGCCCCCGGCTACGACCACATCACCTCCGGCATCGGCGCGGCGATGATCGGCTGGTTCGGCTGCGCCATGCTCTGCTACGTCACGCCCAAGGAGCACCTGGGTCTGCCGAACAAGGATGATGTGAAGACCGGGATCATCACCTACAAGATCGCCGCCCATGCCGCCGACCTCGCCAAGGGCCACCCGGGCGCGCAGATCCGCGACAACGCGCTGTCCAAGGCGCGCTTCGAGTTCCGTTGGGAGGACCAGTTCAACCTTGGCCTGGACCCGGACACCGCGCGGGCGTTCCATGACGAGACCCTGCCGAAGGACTCGGCCAAGGTCGCGCACTTCTGCTCCATGTGCGGACCGAAGTTCTGCTCGATGAAGATCACCCAGGAAGTCCGCGACTACGCCAAGGAGAACGGCCTGTCCGACGAGAGCAAGGCCATCGAGGCCGGCTTCCAGGAGCAGGCGGCGCGCTTCAAGGACGAAGGATCGGTGATCTACAGGCAGGTCTAG
Protein Properties
Protein Residues: 627
Protein Molecular Weight: 69.8 kDa
Protein Theoretical pI: 5.83
Hydropathicity (GRAVY score): -0.364
Charge at pH 7 (predicted): -12.66
Protein Sequence: 
>PA4973
MSATQKNNITRLEQLDRQSTQPFPNSRKVYLTGSRPDIRVPVREISLADTPTAFGGEKNPPVFVYDTSGPYTDPEVRIDLRKGLPDVRSRWIDERGDTEILPGLTSEFGQARLADASLDALRFAHVRTPRRAKPGANVSQMHYAKKGIITPEMEYIAIRENMKLQEARAAGLLDQQHPGHSFGANIPKEITPEFVREEVARGRAIIPANINHTELEPMIIGRNFLVKINGNIGNSALGSSIEEEVEKLTWGIRWGADTVMDLSTGKHIHETREWILRNSPVPIGTVPIYQALEKVNGVAEDLTWEIFRDTLIEQAEQGVDYFTIHAGVLLRYVPLTAKRVTGIVSRGGSIMAKWCLAHHQENFLYTHFEEICEIMKAYDVSFSLGDGLRPGSVADANDAAQFGELETLGELTKIAWKHDVQVMIEGPGHVPMQLIKENMDKQLECCDEAPFYTLGPLTTDIAPGYDHITSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKDDVKTGIITYKIAAHAADLAKGHPGAQIRDNALSKARFEFRWEDQFNLGLDPDTARAFHDETLPKDSAKVAHFCSMCGPKFCSMKITQEVRDYAKENGLSDESKAIEAGFQEQAARFKDEGSVIYRQV
References
External Links:
Resource Link
Genome ID: PA4973
Entrez Gene ID: 879702
NCBI Protein ID: 15600166
General Reference: PaperBLAST - Find papers about PA4973 and its homologs