Methionine synthase (PA1843)

Identification
Name: Methionine synthase
Synonyms:
  • 5-methyltetrahydrofolate--homocysteine methyltransferase
  • Methionine synthase, vitamin-B12-dependent
  • MS
Gene Name: metH
Enzyme Class:
Biological Properties
General Function: Involved in methionine synthase activity
Specific Function: Catalyzes the transfer of a methyl group from methyl- cobalamin to homocysteine, yielding enzyme-bound cob(I)alamin and methionine. Subsequently, remethylates the cofactor using methyltetrahydrofolate
Cellular Location: Not Available
KEGG Pathways:
KEGG Reactions:
Thumb+Thumb ↔ Thumb+Thumb+Thumb
5-Methyltetrahydrofolic acid + L-Homocysteine ↔ Hydrogen ion + L-Methionine + Tetrahydrofolic acid
ReactionCard
Thumb+Thumb ↔ Thumb+Thumb
5-Methyltetrahydrofolic acid + L-Homocysteine ↔ Tetrahydrofolic acid + L-Methionine
ReactionCard
SMPDB Reactions:
5-Methyltetrahydrofolic acid+Homocysteine+Thumb+Thumb → Tetrahydrofolic acid+Thumb+Thumb
5-Methyltetrahydrofolic acid + Homocysteine + 5-Methyltetrahydrofolic acid + Homocysteine → Tetrahydrofolic acid + L-Methionine + Tetrahydrofolic acid
ReactionCard
Homocysteine+Thumb+Thumb → Thumb+Thumb
Homocysteine + N5-methyl--tetrahydropteroyl tri-L-glutamate + Homocysteine → L-Methionine + tetrahydropteroyltri-L-glutamate
ReactionCard
Homocysteine+Thumb+Thumb → Thumb+Thumb
Homocysteine + N5-methyl--tetrahydropteroyl tri-L-glutamate + Homocysteine → tetrahydropteroyltri-L-glutamate + L-Methionine
ReactionCard
PseudoCyc/BioCyc Reactions:
Thumb+Thumb ↔ Thumb+Thumb+Thumb
5-Methyltetrahydrofolic acid + L-Homocysteine ↔ Hydrogen ion + L-Methionine + Tetrahydrofolic acid
ReactionCard
Thumb+Thumb ? Thumb+Thumb
L-Homocysteine + 5-Methyltetrahydrofolic acid ? L-Methionine + Tetrahydrofolic acid
ReactionCard
Complex Reactions:
Thumb+Thumb → Thumb+Thumb
5-Methyltetrahydrofolic acid + L-Homocysteine → Tetrahydrofolic acid + L-Methionine
ReactionCard
Transports: Not Available
Metabolites:
PAMDB IDNameView
PAMDB006316 N5-methyl--tetrahydropteroyl tri-L-glutamateMetaboCard
PAMDB0003685-Methyltetrahydrofolic acidMetaboCard
PAMDB0002455-Methyltetrahydropteroyltri-L-glutamic acidMetaboCard
PAMDB006170HomocysteineMetaboCard
PAMDB001633Hydrogen ionMetaboCard
PAMDB000067L-HomocysteineMetaboCard
PAMDB000175L-MethionineMetaboCard
PAMDB006361N5-methyl--tetrahydropteroyl tri-L-glutamateMetaboCard
PAMDB001428SelenohomocysteineMetaboCard
PAMDB000424Tetrahydrofolic acidMetaboCard
PAMDB003550tetrahydropteroyltri-L-glutamateMetaboCard
PAMDB000283Tetrahydropteroyltri-L-glutamic acidMetaboCard
GO Classification:
Component
cell part
intracellular
Function
binding
catalytic activity
cation binding
cobalamin binding
homocysteine S-methyltransferase activity
ion binding
metal ion binding
methionine synthase activity
methyltransferase activity
S-methyltransferase activity
transferase activity
transferase activity, transferring one-carbon groups
transition metal ion binding
vitamin binding
zinc ion binding
Process
cellular amino acid and derivative metabolic process
cellular amino acid metabolic process
cellular aromatic compound metabolic process
cellular metabolic process
metabolic process
methionine biosynthetic process
pteridine and derivative metabolic process
sulfur amino acid biosynthetic process
sulfur amino acid metabolic process
Gene Properties
Locus tag: PA1843
Strand: -
Entrez Gene ID: 880631
Accession: NP_250534.1
GI: 15597040
Sequence start: 1999889
Sequence End: 2003593
Sequence Length: 3704
Gene Sequence: 
>PA1843
ATGTCCAGCCCGCTCACCGATCGCAGCGCCCGCCTGCAAGCCCTCCAGCACGCCCTCAGGGAACGTATCCTGATCCTCGATGGCGGCATGGGCACCATGATCCAGAGCTACAAGCTGGAAGAGGCCGACTACCGCGGCGAGCGCTTCGCCGACTGGCCGAGCGACGTGAAAGGCAACAACGACCTCTTGCTGCTGAGCCGCCCGGACGTGATCCAGGCCATCGAGAAGGCCTACCTCGACGCCGGCGCCGACATCCTCGAGACCAACACCTTCAACGCCACCCAGGTGTCCCAGGCCGACTACGGCATGCAGTCGCTGGCCTACGAACTCAACGTCGAAGGGGCGCGCCTGGCCCGCCAGGTGGCGGACGCGAAGACCGCCGAGACCCCGGACAAGCCGCGTTTCGTCGCCGGCGTGCTCGGCCCGACCAGCCGCACCTGCTCGATTTCCCCGGACGTGAACAACCCCGGCTACCGCAACGTCACCTTCGACGAACTGGTGGAGAACTACGTCGAGGCGACCCGAGGCCTGATCGAAGGCGGCGCCGACCTGATCCTGATCGAGACCATCTTCGACACCCTCAACGCCAAGGCGGCGATCTTCGCCGTCCAGGGCGTGTTCGAGGAACTCGGCGTGGAGCTGCCGATCATGATCTCCGGAACCATCACCGACGCCTCCGGCCGCACCCTGTCGGGCCAGACCACCGAGGCCTTCTGGAACTCGGTGCGGCATGCCCGGCCGATCTCGGTAGGCCTGAACTGCGCCCTCGGCGCCAAGGAATTGCGGCCGTACATCGAGGAACTGTCGACCAAGGCCGACACTCATGTCTCGGCCCACCCCAACGCCGGCCTGCCGAACGCCTTCGGCGAATACGACGAATCGCCGGCGGAAATGGCCGTGGTGGTCGAGGAATTCGCCGCCGCCGGCTTCCTCAATATCGTCGGCGGCTGCTGCGGCACCACCCCGGCGCACATCGAGGCGATCGCCAAGGCAGTGGCCAAGTACCCGCCGCGGGCCATCCCGGAGATTCCCCGGGCCTGTCGCCTGTCCGGCCTGGAGCCGTTCACCATCGACCGCAGCTCGCTGTTCGTCAACGTCGGCGAGCGCACCAACATCACCGGTTCGGCCAAGTTCGCCCGGCTGATCCGCGAGGAAAACTACGCGGAAGCTCTCGAGGTCGCCCAGCAGCAGGTGGAAGCCGGCGCCCAGGTGATCGACATCAACATGGACGAAGGCATGCTGGACTCGAAGGCGGCCATGGTCACCTTCCTCAACCTGATCGCCTCCGAGCCCGACATCTCGCGCGTGCCGATCATGATCGACTCCTCCAAGTGGGAAGTGATCGAGGCCGGCCTGAAGTGCATCCAGGGCAAGGGCATCGTCAACTCGATCTCGATGAAGGAAGGCGTCGAGGCCTTCAAGCACCATGCCCGCCTGTGCAAGCGCTACGGCGCCGCGGTGGTGGTGATGGCCTTCGACGAGGACGGCCAGGCCGACACCCAGGCGCGCAAGGAAGAAATCTGCAAGCGCTCCTACGACATCCTGGTCGACGAAGTCGGCTTCCCACCGGAAGACATCATCTTCGATGCGAACATCTTCGCCATCGCCACCGGCATCGAGGAACACAACAACTACGCGGTCGATTTCATCAACGCCTGCGCCTACATCCGCGACAACCTCCCCTACGCCCTGAGCTCGGGCGGGGTGTCCAACGTGTCCTTCTCGTTCCGCGGCAACAACCCGGTACGCGAGGCGATCCACTCGGTGTTCCTCTACTACGCGATCCGCAACGGCCTGACCATGGGCATCGTCAACGCCGGCCAGCTGGAAATCTACGACGAGATTCCGAAAGCGCTGCGCGACCGGGTCGAGGACGTGGTGCTCAACCGCACGCCCGAGGCCACCGAGGCCCTGCTGGCGATCGCCGACGACTACAAGGGCGGCGGCGCGGTCAAGGAGGCCGAGGACGAGGAATGGCGCAGCTACAGCGTCGAGAAGCGCCTCGAGCATGCGCTGGTCAAGGGCATCACCACCTGGATCGTCGAGGACACCGAGGAATGCCGCCAGCAGTGTGCGCGTCCCATCGAGGTCATCGAAGGTCCGCTGATGTCCGGGATGAACGTGGTCGGCGACCTGTTCGGCGCCGGCAAGATGTTCCTCCCGCAGGTGGTCAAGTCCGCGCGAGTGATGAAGCAGGCGGTGGCCCACCTGATTCCCTTCATCGAGGCGGAGAAAGGCGACAAGCCGGAAGCCAAGGGCAAGATCCTGATGGCCACGGTGAAGGGCGACGTGCACGACATCGGCAAGAACATCGTCGGCGTGGTGCTCGGCTGCAACGGCTATGACGTGGTCGACCTCGGCGTGATGGTGCCGGCGGAGAAGATCCTGCAGACCGCCATCGCCGAGAAATGCGACATCATCGGCCTGTCTGGCCTGATCACGCCGTCGCTGGACGAGATGGTCCACGTCGCCAAGGAAATGCAGCGGCAGAATTTCCAGTTGCCGCTGATGATCGGCGGCGCCACTACCTCGAAGGCGCATACCGCGGTGAAGATCGATCCGCAGTACAGCAACGACGCGGTGGTCTACGTCACCGACGCCTCGCGCGCGGTAGGCGTGGCCACCAGCCTGCTGTCCAAGGAGCTGAAGGCCGACTACGTGGCCCGCACCCGCGCCGACTACGCGGTGGTCCGCGAACGCACGGCCAACCGCAGCGCCCGCACCGAGCGGCTGAGCTACGAACAGGCGATCGCCAACAAGCCGGCGTTCGACTGGGCCGGCTACCAGGCGCCGACGCCTTCCTTCACCGGCGTCAGGGTGCTCGACGAGATCGACCTCGCGGTGCTCGCCGAGTACATCGACTGGACGCCGTTCTTCATTTCCTGGGACCTGGCCGGCAAGTACCCGCGCATCCTCACCGACGAGGTGGTCGGCGAGGCCGCCACCTCGTTGTTCAACGACGCCCAGGCGATGCTGAAGAAGCTGATCGACGAGAAGCTGATCAAGGCCCGCGCGGTGTTCGGCTTCTGGCCGGCCAACCAGGTCGAGCACGACGACCTGGAGGTCTACGGCGCCGATGGCGAGACCCTCGCCACCCTGCACCACCTGCGGCAGCAGACGATCAAGCCGGACGGCAAGCCGAACCTGTCGCTGGCCGATTTCGTCGCGCCGAAGGAAAGCGGCGTGCGCGACTACATCGGCGGCTTCATCACCACCGCCGGGATCGGCGCCGAGGAAGTGGCCAAGGCGTACGAAGCCAAGGGCGACGACTACAACAGCATCATGGTCAAGGCGCTCGCCGACCGCCTCGCCGAAGCCTGCGCCGAGTGGCTGCACGAGCGGGTGCGCAAGGAGTACTGGGGCTACGCCCGCGACGAACACCTCGACAACGAGGCCTTGATCAAGGAGCAATACGTCGGCATCCGCCCGGCACCGGGCTACCCGGCCTGCCCCGACCATACCGAGAAAGGCACTCTGTTCGAACTGCTCGATCCGCAGGGCCTGTCCGGCGTCAGCCTGACCGAGCACTACGCGATGTTCCCGGCCGCGGCGGTCAGCGGTTGGTATTTCGCCCACCCGCAGGCGCAGTACTTCGCGGTCGGCAAGATCGACAAGGACCAGGTGGAACGCTACAGCCAGCGCAAGGGCCAGGAAGCCAGCGTCAGCGAGCGCTGGCTGGCGCCGAACCTTGGCTACGATGACTGA
Protein Properties
Protein Residues: 1234
Protein Molecular Weight: 135.1 kDa
Protein Theoretical pI: 4.62
Hydropathicity (GRAVY score): -0.154
Charge at pH 7 (predicted): -53.05
Protein Sequence: 
>PA1843
MSSPLTDRSARLQALQHALRERILILDGGMGTMIQSYKLEEADYRGERFADWPSDVKGNNDLLLLSRPDVIQAIEKAYLDAGADILETNTFNATQVSQADYGMQSLAYELNVEGARLARQVADAKTAETPDKPRFVAGVLGPTSRTCSISPDVNNPGYRNVTFDELVENYVEATRGLIEGGADLILIETIFDTLNAKAAIFAVQGVFEELGVELPIMISGTITDASGRTLSGQTTEAFWNSVRHARPISVGLNCALGAKELRPYIEELSTKADTHVSAHPNAGLPNAFGEYDESPAEMAVVVEEFAAAGFLNIVGGCCGTTPAHIEAIAKAVAKYPPRAIPEIPRACRLSGLEPFTIDRSSLFVNVGERTNITGSAKFARLIREENYAEALEVAQQQVEAGAQVIDINMDEGMLDSKAAMVTFLNLIASEPDISRVPIMIDSSKWEVIEAGLKCIQGKGIVNSISMKEGVEAFKHHARLCKRYGAAVVVMAFDEDGQADTQARKEEICKRSYDILVDEVGFPPEDIIFDANIFAIATGIEEHNNYAVDFINACAYIRDNLPYALSSGGVSNVSFSFRGNNPVREAIHSVFLYYAIRNGLTMGIVNAGQLEIYDEIPKALRDRVEDVVLNRTPEATEALLAIADDYKGGGAVKEAEDEEWRSYSVEKRLEHALVKGITTWIVEDTEECRQQCARPIEVIEGPLMSGMNVVGDLFGAGKMFLPQVVKSARVMKQAVAHLIPFIEAEKGDKPEAKGKILMATVKGDVHDIGKNIVGVVLGCNGYDVVDLGVMVPAEKILQTAIAEKCDIIGLSGLITPSLDEMVHVAKEMQRQNFQLPLMIGGATTSKAHTAVKIDPQYSNDAVVYVTDASRAVGVATSLLSKELKADYVARTRADYAVVRERTANRSARTERLSYEQAIANKPAFDWAGYQAPTPSFTGVRVLDEIDLAVLAEYIDWTPFFISWDLAGKYPRILTDEVVGEAATSLFNDAQAMLKKLIDEKLIKARAVFGFWPANQVEHDDLEVYGADGETLATLHHLRQQTIKPDGKPNLSLADFVAPKESGVRDYIGGFITTAGIGAEEVAKAYEAKGDDYNSIMVKALADRLAEACAEWLHERVRKEYWGYARDEHLDNEALIKEQYVGIRPAPGYPACPDHTEKGTLFELLDPQGLSGVSLTEHYAMFPAAAVSGWYFAHPQAQYFAVGKIDKDQVERYSQRKGQEASVSERWLAPNLGYDD
References
External Links:
Resource Link
Genome ID: PA1843
Entrez Gene ID: 880631
NCBI Protein ID: 15597040
General Reference: PaperBLAST - Find papers about PA1843 and its homologs