Identification
Name: Fatty acid oxidation complex subunit alpha
Synonyms:
  • Enoyl-CoA hydratase/Delta(3)-cis-Delta(2)-trans-enoyl-CoA isomerase/3-hydroxybutyryl-CoA epimerase
  • 3-hydroxyacyl-CoA dehydrogenase
Gene Name: fadB
Enzyme Class:
Biological Properties
General Function: Involved in 3-hydroxyacyl-CoA dehydrogenase activity
Specific Function: Catalyzes the formation of an hydroxyacyl-CoA by addition of water on enoyl-CoA. Also exhibits 3-hydroxyacyl-CoA epimerase and 3-hydroxyacyl-CoA dehydrogenase activities. Involved in the aerobic and anaerobic degradation of long-chain fatty acids
Cellular Location: Not Available
KEGG Pathways:
Transports:
Transport References:
  • Orth, J. D., Conrad, T. M., Na, J., Lerman, J. A., Nam, H., Feist, A. M., Palsson, B. O. (2011). "A comprehensive genome-scale reconstruction of Escherichia coli metabolism--2011." Mol Syst Biol 7:535. Pubmed: 21988831
KEGG Reactions: Not Available
SMPDB Reactions: Not Available
PseudoCyc/BioCyc Reactions:
ThumbThumb+Thumb
Thumb+Thumb+ThumbThumb+Thumb
ThumbThumb+Thumb
ThumbThumb+Thumb
Thumb+Thumb+ThumbThumb+Thumb
ThumbThumb+Thumb
Thumb+Thumb+ThumbThumb+Thumb
ThumbThumb+Thumb
Thumb+Thumb+ThumbThumb+Thumb
ThumbThumb+Thumb
Thumb+Thumb+ThumbThumb+Thumb
ThumbThumb+Thumb
Thumb+Thumb+ThumbThumb+Thumb
Thumb+Thumb+ThumbThumb+Thumb
ThumbThumb+Thumb
Thumb+Thumb+ThumbThumb+Thumb
PAMDB120265+PAMDB110542PAMDB120027+PAMDB110540+PAMDB001633
PAMDB120021PAMDB120517+PAMDB000142
Thumb+ThumbThumb
Thumb+ThumbThumb+Thumb+Thumb
ThumbThumb
Thumb+ThumbThumb+Thumb+Thumb
ThumbThumb+Thumb
ThumbThumb
ThumbThumb+Thumb
Thumb+ThumbThumb
Thumb+ThumbThumb+Thumb+Thumb
Thumb+ThumbThumb+Thumb
Thumb+ThumbThumb+Thumb+Thumb
Thumb+ThumbThumb+Thumb+Thumb
Thumb+ThumbThumb+Thumb+Thumb
(S)-3-Hydroxyisobutyrate+ThumbThumb+Thumb+Thumb
Thumb+ThumbThumb+Thumb+Thumb
(6Z,9Z,12Z,15Z,18Z,21Z)-3-Hydroxytetracosahexa-6,9,12,15,18,21-enoyl-CoA(2E,6Z,9Z,12Z,15Z,18Z,21Z)-Tetracosahepta-2,6,9,12,15,18,21-enoyl-CoA+Thumb
(6Z,9Z,12Z,15Z,18Z,21Z)-3-Hydroxytetracosahexa-6,9,12,15,18,21-enoyl-CoA ↔ (2E,6Z,9Z,12Z,15Z,18Z,21Z)-Tetracosahepta-2,6,9,12,15,18,21-enoyl-CoA + Water
ReactionCard
(6Z,9Z,12Z,15Z,18Z)-3-Hydroxytetracosapenta-6,9,12,15,18-enoyl-CoA(2E,6Z,9Z,12Z,15Z,18Z)-Tetracosahexa-2,6,9,12,15,18-enoyl-CoA+Thumb
(6Z,9Z,12Z,15Z,18Z)-3-Hydroxytetracosapenta-6,9,12,15,18-enoyl-CoA ↔ (2E,6Z,9Z,12Z,15Z,18Z)-Tetracosahexa-2,6,9,12,15,18-enoyl-CoA + Water
ReactionCard
Thumb+ThumbThumb
Thumb+ThumbThumb+Thumb+Thumb
(S)-3-hydroxyacyl-CoA+Thumb3-oxoacyl-CoA+Thumb
(S)-3-hydroxyacyl-CoA + NAD → 3-oxoacyl-CoA + NADH
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(3S)-3-hydroxyacyl-CoAtrans-2(or 3)-enoyl-CoA+Thumb
(3S)-3-hydroxyacyl-CoA → trans-2(or 3)-enoyl-CoA + Water
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Thumb(R)-3-hydroxybutanoyl-CoA
3-Hydroxybutyryl-CoA → (R)-3-hydroxybutanoyl-CoA
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(3S)-3-Hydroxyacyl-CoAThumb+trans-3-Enoyl-CoA+Thumb
(3S)-3-Hydroxyacyl-CoA ↔ trans-2,3-Dehydroacyl-CoA + trans-3-Enoyl-CoA + Water
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(3S)-3-Hydroxyacyl-CoA+ThumbThumb+Thumb+Thumb
(3S)-3-Hydroxyacyl-CoA + NAD3-Oxoacyl-CoA + NADH + Hydrogen ion
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PAMDB120596+PAMDB110542PAMDB120590+PAMDB110540+PAMDB001633
trans-2-cis-5-dienoyl-CoAtrans-3-cis-5-dienoyl-CoA
trans-2-cis-5-dienoyl-CoA → trans-3-cis-5-dienoyl-CoA
ReactionCard
Trans-3-enoyl-CoAsTRANS-D2-ENOYL-COA
Trans-3-enoyl-CoAs → TRANS-D2-ENOYL-COA
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PAMDB120070PAMDB120059
PAMDB110396PAMDB110395
D-3-HYDROXYACYL-COAL-3-HYDROXYACYL-COA
D-3-HYDROXYACYL-COA → L-3-HYDROXYACYL-COA
ReactionCard
PAMDB120145PAMDB120517+PAMDB000142
PAMDB120180PAMDB120140+PAMDB000142
L-3-HYDROXYACYL-COA+PAMDB1105423-KETOACYL-COA+PAMDB110540+PAMDB001633
L-3-HYDROXYACYL-COA + NAD+ → 3-KETOACYL-COA + NADH + Hydrogen ion
ReactionCard
PAMDB120234PAMDB120187+PAMDB000142
PAMDB120180+PAMDB110542PAMDB120306+PAMDB110540+PAMDB001633
PAMDB120354+PAMDB110542PAMDB120340+PAMDB110540+PAMDB001633
PAMDB120324+PAMDB000142PAMDB120265
PAMDB120508+PAMDB110542PAMDB120367+PAMDB110540+PAMDB001633
PAMDB120354PAMDB120318+PAMDB000142
PAMDB120075+PAMDB110542PAMDB120400+PAMDB110540+PAMDB001633
PAMDB120391+PAMDB110542PAMDB120369+PAMDB110540+PAMDB001633
PAMDB120417+PAMDB110542PAMDB120345+PAMDB110540+PAMDB001633
D-3-HYDROXYACYL-COACis-2-enoyl-CoAs+PAMDB000142
D-3-HYDROXYACYL-COA → Cis-2-enoyl-CoAs + Water
ReactionCard
Long-Chain-3S-Hydroxyacyl-CoAs+PAMDB110542Long-Chain-oxoacyl-CoAs+PAMDB110540+PAMDB001633
Long-Chain-3S-Hydroxyacyl-CoAs + NAD+ → Long-Chain-oxoacyl-CoAs + NADH + Hydrogen ion
ReactionCard
PAMDB120480+PAMDB110542PAMDB120469+PAMDB110540+PAMDB001633
L-3-HYDROXYACYL-COATRANS-D2-ENOYL-COA+PAMDB000142
L-3-HYDROXYACYL-COA → TRANS-D2-ENOYL-COA + Water
ReactionCard
PAMDB120075PAMDB120028+PAMDB000142
PAMDB120234+PAMDB110542PAMDB001633+PAMDB110398+PAMDB110540
PAMDB120508PAMDB120470+PAMDB000142
PAMDB120564+PAMDB110542PAMDB120541+PAMDB110540+PAMDB001633
Long-Chain-3S-Hydroxyacyl-CoAsLong-Chain-Trans-23-Dehydroacyl-CoA+PAMDB000142
Long-Chain-3S-Hydroxyacyl-CoAs → Long-Chain-Trans-23-Dehydroacyl-CoA + Water
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CIS-DELTA3-ENOYL-COATRANS-D2-ENOYL-COA
CIS-DELTA3-ENOYL-COA → TRANS-D2-ENOYL-COA
ReactionCard
Complex Reactions: Not Available
Transports:
Metabolites:
PAMDB IDNameView
PAMDB000857(2E)-5-Methylhexa-2,4-dienoyl-CoAMetaboCard
PAMDB000543(2E)-Decenoyl-CoAMetaboCard
PAMDB000522(2E)-Dodecenoyl-CoAMetaboCard
PAMDB000541(2E)-Hexadecenoyl-CoAMetaboCard
PAMDB000544(2E)-Octenoyl-CoAMetaboCard
PAMDB000542(2E)-Tetradecenoyl-CoAMetaboCard
PAMDB000859(3S)-3-Hydroxyadipyl-CoAMetaboCard
PAMDB003540(3Z)-Dodec-3-enoyl-CoAMetaboCard
PAMDB002036(S)-3-Hydroxybutanoyl-CoAMetaboCard
PAMDB000532(S)-3-Hydroxydodecanoyl-CoAMetaboCard
PAMDB000529(S)-3-Hydroxyhexadecanoyl-CoAMetaboCard
PAMDB000234(S)-3-Hydroxyisobutyryl-CoAMetaboCard
PAMDB001510(S)-3-Hydroxyoctadecanoyl-CoAMetaboCard
PAMDB000530(S)-3-Hydroxytetradecanoyl-CoAMetaboCard
PAMDB000534(S)-Hydroxydecanoyl-CoAMetaboCard
PAMDB000538(S)-Hydroxyhexanoyl-CoAMetaboCard
PAMDB000536(S)-Hydroxyoctanoyl-CoAMetaboCard
PAMDB000448(S)-Methylmalonic acid semialdehydeMetaboCard
PAMDB0002772-Methylacetoacetyl-CoAMetaboCard
PAMDB0009203-Hydroxy-5-methylhex-4-enoyl-CoAMetaboCard
PAMDB0002803-Hydroxybutyryl-CoAMetaboCard
PAMDB0006983-Hydroxyisovaleryl-CoAMetaboCard
PAMDB0004013-Methylcrotonyl-CoAMetaboCard
PAMDB0009263-Oxoadipyl-CoAMetaboCard
PAMDB0005353-Oxodecanoyl-CoAMetaboCard
PAMDB0005333-Oxododecanoyl-CoAMetaboCard
PAMDB0006693-Oxohexadecanoyl-CoAMetaboCard
PAMDB0005393-Oxohexanoyl-CoAMetaboCard
PAMDB0015793-Oxooctadecanoyl-CoAMetaboCard
PAMDB0005373-Oxooctanoyl-CoAMetaboCard
PAMDB0005313-Oxotetradecanoyl-CoAMetaboCard
PAMDB0009495-Carboxy-2-pentenoyl-CoAMetaboCard
PAMDB0009525-Methyl-3-oxo-4-hexenoyl-CoAMetaboCard
PAMDB000397Acetoacetyl-CoAMetaboCard
PAMDB000455Acrylyl-CoAMetaboCard
PAMDB000429Crotonoyl-CoAMetaboCard
PAMDB001633Hydrogen ionMetaboCard
PAMDB000225Methacrylyl-CoAMetaboCard
PAMDB000197NADMetaboCard
PAMDB000398NADHMetaboCard
PAMDB001668Octadecenoyl-CoA (N-C18:1CoA)MetaboCard
PAMDB000433Tiglyl-CoAMetaboCard
PAMDB000812trans,cis-Lauro-2,6-dienoyl-CoAMetaboCard
PAMDB000540trans-2-Hexenoyl-CoAMetaboCard
PAMDB001759Trans-Octadec-2-enoyl-CoAMetaboCard
PAMDB000142WaterMetaboCard
GO Classification:
Component
fatty acid beta-oxidation multienzyme complex
macromolecular complex
protein complex
Function
3-hydroxyacyl-CoA dehydrogenase activity
3-hydroxybutyryl-CoA epimerase activity
binding
carbon-oxygen lyase activity
catalytic activity
coenzyme binding
cofactor binding
dodecenoyl-CoA delta-isomerase activity
enoyl-CoA hydratase activity
hydro-lyase activity
intramolecular oxidoreductase activity
intramolecular oxidoreductase activity, transposing C=C bonds
isomerase activity
lyase activity
oxidoreductase activity
oxidoreductase activity, acting on CH-OH group of donors
oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
racemase and epimerase activity
racemase and epimerase activity, acting on hydroxy acids and derivatives
Process
carboxylic acid metabolic process
cellular metabolic process
fatty acid catabolic process
fatty acid metabolic process
lipid catabolic process
lipid metabolic process
metabolic process
monocarboxylic acid metabolic process
organic acid metabolic process
oxidation reduction
oxoacid metabolic process
primary metabolic process
Gene Properties
Locus tag: PA3014
Strand: -
Entrez Gene ID: 878680
Accession: NP_251704.1
GI: 15598210
Sequence start: 3374460
Sequence End: 3376607
Sequence Length: 2147
Gene Sequence:
>PA3014
ATGATTTACCAAGGTAAAGCCATCACGGTTAAGCCTCTTGAGGGCGGCATCGTCGAGTTGAATTTCGATCTCAAGGGCGAGTCCGTCAACAAGTTCAACCGTCTCACCCTCAGTGAGTTGCGTGCGGCAGTCGATGCGATCAAGGCCGATGCATCGGTCAAGGGCGTGATCGTGACCAGCGGCAAGGACGTGTTCATCGTTGGTGCCGACATCACCGAGTTCGTTGACAACTTCCAGCTGCCCGACGAGGAACTGATGGCGGGCAACCTCGAAGCCAACAAGATCTTCAGCGACTTCGAAGACCTGGACGTACCCACCGTTGCCGCGATCAACGGCATCGCCCTCGGCGGCGGCCTGGAGATGTGCCTGGCCGCGGACTTCCGCGTGATGAGCGCGACCGCCAAGGTCGGCCTGCCGGAAGTCAAGCTGGGCATCTACCCGGGCTTCGGCGGCACCGTCCGCCTGCCGCGCCTGATCGGCTGCGATAACGCCGTCGAGTGGATCGCCTCGGGCAAGGAAAACAAGGCCGAGGACGCCCTGAAGGTCGGCGCCGTCGATGCCGTGGTCGCTCCCGAGCAACTGCAGGCCGCTGCCCTCGACCTGGCCAAGCGCGCCGTCGCCGGCGAGCTGGACCACAAGGCACGGCGCCAGCCGAAGCTGGAAAAGCTGAAGCTGAATGCAATCGAGCAGATGATGGCCTTCGAAACCGCCAAGGGCTTCGTCGCCGGCCAGGCCGGTCCGAACTACCCGGCGCCGGTGGAAGCCATCAAGAGCATCCAGAAGGCCGCCAACTTCGGCCGCGACAAGGCGCTGGAAGTCGAAGCCGCCGGCTTCGTCAAGCTGGCCAAGACCTCGGTCGCGCAGAGTCTGATCGGCCTGTTCCTGAATGACCAGGAACTGAAGAAGAAAGCCAAGAAATACGACGAAGTCGCCAAGGACGTGAAACTGGCCGCCGTGCTCGGCGCCGGCATCATGGGCGGCGGCATCGCCTATCAGTCGGCGCTCAAGGGCACCCCGATCCTGATGAAGGATATCCGCGAAGAGGGTATCCAGATGGGCCTGAACGAGGCCGCCAAGCTGCTCGGCAAGCGCGTCGAGAAGGGTCGCCTGACCCCGGCGAAGATGGCCGAGGCGCTCAATGGCATCCGTCCGACCATGTCCTATGGCGACTTCGGCAACGTCGACATCGTCGTCGAAGCCGTCGTCGAGAACCCCAAGGTCAAGCAAGCCGTGCTCGCCGAAGTGGAAGGCGCGGTGAAGGAAGACGCGATCATCGCCTCCAACACTTCGACCATCTCCATCAGCCTGCTGGCCCAGGCGCTGAAGCGTCCGGAAAACTTCTGCGGCATGCATTTCTTCAACCCGGTGCACATGATGCCGCTGGTGGAAGTGATCCGTGGCGAGAAGACCGGTGAAACCGCCATCGCCACCACCGTCGCCTACGCCAAGAAGATGGGCAAGAGCCCGATCGTGGTCAACGATTGCCCAGGCTTCCTGGTCAACCGTGTGCTGTTCCCGTACTTCGGCGGCTTCGCCAAGCTGCTGAGCTTCGGCGTCGACTTCGTGCGCATCGACAAGGTGATGGAGAAGTTCGGCTGGCCCATGGGCCCGGCCTACCTGTCCGACGTGGTCGGCATCGACACCGGCCACCATGGCCGCGACGTCATGGCCGAGGGCTTCCCGGACCGCATGGCGGTCGAGGGCAAGACCGCCGTCGACGTGATGTACGAGGCCAACCGCCTGGGCCAGAAGAACGGCAAGGGCTTCTACGCCTACGAGACCGACAAGCGCGGCAAGCCGAAGAAGGTCACCGATCCGCAGGCCTACGAAGTGCTCAAGCCGATCGTGGTCGAGCAGCGCGAAGTGACCGACGAGGACATCGTCAACTTCATGATGATCCCGCTGTGCCTGGAAACCGTGCGTTGCCTGGAAGACGGCATCGTCGAGACCGCTGCCGAGGCCGATATGGGCCTGATCTACGGCATCGGCTTCCCGCCCTTCCGTGGTGGTGCTCTGCGCTACATCGATTCGATCGGTGTCGCCGAGTTCGTGGCCCTGGCCGACAAGTACGCCGAGCTGGGCGCGCTGTACCACCCGACCGCGAAGCTGCGTGAAATGGCCAAGAACGGTCAGAAATTCTTCGGTTAA
Protein Properties
Protein Residues: 715
Protein Molecular Weight: 77 kDa
Protein Theoretical pI: 5.57
Hydropathicity (GRAVY score): 0.026
Charge at pH 7 (predicted): -6.69
Protein Sequence:
>PA3014
MIYQGKAITVKPLEGGIVELNFDLKGESVNKFNRLTLSELRAAVDAIKADASVKGVIVTSGKDVFIVGADITEFVDNFQLPDEELMAGNLEANKIFSDFEDLDVPTVAAINGIALGGGLEMCLAADFRVMSATAKVGLPEVKLGIYPGFGGTVRLPRLIGCDNAVEWIASGKENKAEDALKVGAVDAVVAPEQLQAAALDLAKRAVAGELDHKARRQPKLEKLKLNAIEQMMAFETAKGFVAGQAGPNYPAPVEAIKSIQKAANFGRDKALEVEAAGFVKLAKTSVAQSLIGLFLNDQELKKKAKKYDEVAKDVKLAAVLGAGIMGGGIAYQSALKGTPILMKDIREEGIQMGLNEAAKLLGKRVEKGRLTPAKMAEALNGIRPTMSYGDFGNVDIVVEAVVENPKVKQAVLAEVEGAVKEDAIIASNTSTISISLLAQALKRPENFCGMHFFNPVHMMPLVEVIRGEKTGETAIATTVAYAKKMGKSPIVVNDCPGFLVNRVLFPYFGGFAKLLSFGVDFVRIDKVMEKFGWPMGPAYLSDVVGIDTGHHGRDVMAEGFPDRMAVEGKTAVDVMYEANRLGQKNGKGFYAYETDKRGKPKKVTDPQAYEVLKPIVVEQREVTDEDIVNFMMIPLCLETVRCLEDGIVETAAEADMGLIYGIGFPPFRGGALRYIDSIGVAEFVALADKYAELGALYHPTAKLREMAKNGQKFFG
References
External Links:
Resource Link
Genome ID: PA3014
Entrez Gene ID: 878680
NCBI Protein ID: 15598210
General Reference: PaperBLAST - Find papers about PA3014 and its homologs