7-alpha-hydroxysteroid dehydrogenase (PA4022)

Identification
Name: 7-alpha-hydroxysteroid dehydrogenase
Synonyms:
  • 7-alpha-HSDH
Gene Name: hdhA
Enzyme Class:
Biological Properties
General Function: Involved in oxidoreductase activity
Specific Function: 7-alpha-dehydroxylation of cholic acid, yielding deoxycholic acid and lithocholic acid, respectively. Highest affinity with taurochenodeoxycholic acid
Cellular Location: Not Available
KEGG Pathways: Not Available
KEGG Reactions:
Thumb+Thumb ↔ Thumb+Thumb
Cholic acid + NAD ↔ NADH + Hydrogen ion
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SMPDB Reactions: Not Available
PseudoCyc/BioCyc Reactions:
Thumb+Thumb ↔ Thumb+Thumb
Cholic acid + NAD ↔ NADH + Hydrogen ion
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PAMDB000217+PAMDB110541+PAMDB000142 → PAMDB110762+PAMDB110539+PAMDB001633
Acetaldehyde + NADP+ + Water → acetate + NADPH + Hydrogen ion
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PAMDB120585+PAMDB000142+PAMDB110542 → PAMDB120067+PAMDB110540+PAMDB001633
benzaldehyde + Water + NAD+ → benzoate + NADH + Hydrogen ion
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PAMDB120034+PAMDB110542+PAMDB000142 → PAMDB110740+PAMDB110540+PAMDB001633
L-lactaldehyde + NAD+ + Water → (S)-lactate + NADH + Hydrogen ion
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PAMDB120152+PAMDB000142+PAMDB110542 → PAMDB120128+PAMDB110540+PAMDB001633
4-acetamidobutanal + Water + NAD+ → 4-acetamidobutanoate + NADH + Hydrogen ion
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PAMDB000217+PAMDB000142+PAMDB110542 → PAMDB110762+PAMDB110540+PAMDB001633
Acetaldehyde + Water + NAD+ → acetate + NADH + Hydrogen ion
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PAMDB120368+NAD-P-OR-NOP+PAMDB000142 → PAMDB120348+PAMDB001633+PAMDB110539
apo-4'-lycopenal + NAD-P-OR-NOP + Water → apo-4'-lycopenoate + Hydrogen ion + NADPH
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PAMDB120585+PAMDB000142+PAMDB110541 → PAMDB120067+PAMDB110539+PAMDB001633
benzaldehyde + Water + NADP+ → benzoate + NADPH + Hydrogen ion
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PAMDB120342+PAMDB110542+PAMDB000142 → PAMDB120312+PAMDB110540+PAMDB001633
phytenal + NAD+ + Water → phytenate + NADH + Hydrogen ion
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2-Me-Branched-234-Sat-FALD+PAMDB110542+PAMDB000142 → 2-Me-Branched-234-Sat-FA+PAMDB110540+PAMDB001633
2-Me-Branched-234-Sat-FALD + NAD+ + Water → 2-Me-Branched-234-Sat-FA + NADH + Hydrogen ion
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Odd-Straight-Chain-234-Sat-FALD+PAMDB110542+PAMDB000142 → Odd-Straight-Chain-234-Sat-FA+PAMDB110540+PAMDB001633
Odd-Straight-Chain-234-Sat-FALD + NAD+ + Water → Odd-Straight-Chain-234-Sat-FA + NADH + Hydrogen ion
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Aldehydes+PAMDB110542+PAMDB000142 → Carboxylates+PAMDB110540+PAMDB001633
Aldehydes + NAD+ + Water → Carboxylates + NADH + Hydrogen ion
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Aldehydes+PAMDB110541+PAMDB000142 → Carboxylates+PAMDB110539+PAMDB001633
Aldehydes + NADP+ + Water → Carboxylates + NADPH + Hydrogen ion
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PAMDB120041+PAMDB110542+PAMDB000142 → PAMDB110449+PAMDB110540+PAMDB001633
salicylaldehyde + NAD+ + Water → salicylate + NADH + Hydrogen ion
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Complex Reactions: Not Available
Transports: Not Available
Metabolites:
PAMDB IDNameView
PAMDB0035573-Alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholanateMetaboCard
PAMDB001819Cholic acidMetaboCard
PAMDB000197NADMetaboCard
PAMDB000398NADHMetaboCard
GO Classification:
Function
binding
catalytic activity
oxidoreductase activity
Process
metabolic process
oxidation reduction
Gene Properties
Locus tag: PA4022
Strand: +
Entrez Gene ID: 879017
Accession: NP_252711.1
GI: 15599217
Sequence start: 4502271
Sequence End: 4503791
Sequence Length: 1520
Gene Sequence: 
>PA4022
ATGCGTTACGCCCATCCCGGTAGCGAAGGCGCCATCGTTTCCTTCAAGGCCCGCTACGGCAACTACATCGGCGGCGAGTTCGTGCCTCCGGTCAAGGGCCAGTACTTCACCAACACCTCGCCGGTGAACGGCCAGCCGATCGCCGAGTTCCCCCGTTCCACCGCCGAGGACATCGACAAGGCCCTCGACGCCGCCCACGCCGCCGCCGACGCCTGGGGCCGCACCTCGGTGCAGGAGCGCTCGAACATCCTGCTGAAGATCGCCGACCGCATCGAGCAGAACCTGGAACTGCTCGCCGTCACCGAGACCTGGGACAACGGCAAGGCCGTGCGCGAGACCCTCAACGCCGACATCCCCCTGGCCGCCGACCACTTCCGCTACTTCGCTGGCTGCATTCGCGCCCAGGAAGGCTCCGCCGCCGAGATCAACGACAGCACCGTGGCCTACCACATCCATGAGCCGCTGGGCGTGGTCGGGCAGATCATCCCGTGGAACTTCCCGCTGCTGATGGCCGCCTGGAAGCTGGCCCCGGCGCTGGCCGCCGGCAACTGCGTGGTGCTCAAGCCGGCCGAGCAGACCCCGCTGGGCATCTGCGTGCTGCTGGAACTCATCGGCGACCTGCTGCCGCCGGGCGTGCTCAATGTCGTCCAGGGCTTCGGCAGGGAGGCCGGCGAGGCCCTGGCCACCAGCAAGCGCATCGCCAAGATCGCCTTCACCGGTTCCACCCCGGTCGGCTCGCACATCCTCAAGTGCGCGGCGGAGAACATCATTCCCTCCACCGTCGAGTTGGGCGGCAAGAGCCCGAACATCTATTTCGAAGACATCATGCAGGCCGAGCCGGCGTTCATCGAGAAGGCCGCCGAGGGCCTGGTGCTGGCCTTCTTCAACCAGGGCGAGGTGTGCACCTGCCCGTCCCGCGCGCTGGTCCAGGAGTCGATCTACCCGGCGTTCATGGAAGAGGTGCTGAAAAAGGTCAGGGCGATCAAGCGCGGCGACCCGCTGGACACCGAGACCATGGTCGGCGCCCAGGCTTCCCAGCAGCAGTACGAGAAGATCCTCTCCTACCTCGACATCGCCCAGCAGGAAGGCGCCGAGCTGCTCGCCGGCGGCAGCGTCGAGAAGCTCGAAGGCAACCTGGCCAGCGGCTACTACATCCAGCCGACCCTGCTCAAGGGACACAACGGCATGCGCGTGTTCCAGGAGGAAATCTTCGGCCCGGTGGTCGGCGTCACCACCTTCAAGGACGAAGCCGAGGCCCTGGCCATCGCCAACGACACCGAGTACGGCCTCGGCGCCGGCCTCTGGACCCGCGACATCAACCGCGCCTACCGCATGGGCCGCGGGATCAAGGCCGGTCGCGTGTGGACCAACTGCTACCACCTGTACCCGGCCCACGCCGCGTTCGGCGGCTACAAGAAGTCCGGCGTCGGTCGCGAGACGCACAAGATGATGCTCGACCACTACCAGCAGACCAAGAACCTGCTGGTGAGCTACGACATCAACCCGCTGGGCTTCTTCTAG
Protein Properties
Protein Residues: 506
Protein Molecular Weight: 55 kDa
Protein Theoretical pI: 5.49
Hydropathicity (GRAVY score): -0.079
Charge at pH 7 (predicted): -9.55
Protein Sequence: 
>PA4022
MRYAHPGSEGAIVSFKARYGNYIGGEFVPPVKGQYFTNTSPVNGQPIAEFPRSTAEDIDKALDAAHAAADAWGRTSVQERSNILLKIADRIEQNLELLAVTETWDNGKAVRETLNADIPLAADHFRYFAGCIRAQEGSAAEINDSTVAYHIHEPLGVVGQIIPWNFPLLMAAWKLAPALAAGNCVVLKPAEQTPLGICVLLELIGDLLPPGVLNVVQGFGREAGEALATSKRIAKIAFTGSTPVGSHILKCAAENIIPSTVELGGKSPNIYFEDIMQAEPAFIEKAAEGLVLAFFNQGEVCTCPSRALVQESIYPAFMEEVLKKVRAIKRGDPLDTETMVGAQASQQQYEKILSYLDIAQQEGAELLAGGSVEKLEGNLASGYYIQPTLLKGHNGMRVFQEEIFGPVVGVTTFKDEAEALAIANDTEYGLGAGLWTRDINRAYRMGRGIKAGRVWTNCYHLYPAHAAFGGYKKSGVGRETHKMMLDHYQQTKNLLVSYDINPLGFF
References
External Links:
Resource Link
Genome ID: PA4022
Entrez Gene ID: 879017
NCBI Protein ID: 15599217
General Reference: PaperBLAST - Find papers about PA4022 and its homologs