P. aeruginosa Metabolome Database Protein PA4988

Identification

Name:

3-deoxy-D-manno-octulosonic-acid transferase

Synonyms:

KDO transferase

Gene Name:

waaA

Enzyme Class:

2.4.99.12

Biological Properties

General Function:

Involved in biosynthetic process

Specific Function:

Essential step in lipopolysaccharides biosynthesis. Acts at transfer of 3-deoxy-D-mono octulonic acid (KDO) from CMP-KDO to a tetraacyldisaccharide 1,4'-bisphosphate precursor of lipid A (lipid IVA). Transfers two molecules of KDO to lipid IVA. Degraded by FtsH; therefore FtsH regulates the addition of the sugar moiety of the LPS and thus the maturation of the LPS precursor

Cellular Location:

Cell inner membrane; Single-pass membrane protein (Probable)

KEGG Pathways:

Lipopolysaccharide biosynthesis pae00540
Metabolic pathways pae01100

KEGG Reactions:

↔

2,3,2'3'-Tetrakis(3-hydroxytetradecanoyl)-D-glucosaminyl-1,6-beta-D-glucosamine 1,4'-bisphosphate + CMP-3-Deoxy-D-manno-octulosonate + 2,3,2'3'-Tetrakis(beta-hydroxymyristoyl)-D-glucosaminyl-1,6-beta-D-glucosamine 1,4'-bisphosphate ↔ KDO-lipid IV(A) + Cytidine monophosphate
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↔

Di[3-deoxy-D-manno-octulosonyl]-lipid IV(A)

KDO-lipid IV(A) + CMP-3-Deoxy-D-manno-octulosonate ↔ Di[3-deoxy-D-manno-octulosonyl]-lipid IV(A) + Cytidine monophosphate
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Di[3-deoxy-D-manno-octulosonyl]-lipid IV(A)

↔

alpha-Kdo-(2->8)-alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-lipid IVA

Di[3-deoxy-D-manno-octulosonyl]-lipid IV(A) + CMP-3-Deoxy-D-manno-octulosonate ↔ alpha-Kdo-(2->8)-alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-lipid IVA + Cytidine monophosphate
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SMPDB Reactions:

→

Cytidine monophosphate

CMP-3-Deoxy-D-manno-octulosonate + (2-N,3-O-bis(3-Hydroxytetradecanoyl)-4-O-phosphono-beta-D-glucosaminyl)-(1->6)-(2-N,3-O-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl phosphate) → Cytidine monophosphate + Hydrogen ion + alpha-Kdo-(2??)-lipid IVA + Cytidine monophosphate
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→

Cytidine monophosphate

a-Kdo-(2->4)-a-Kdo-(2->6)-lipid IVA

alpha-Kdo-(2??)-lipid IVA + CMP-3-Deoxy-D-manno-octulosonate → Cytidine monophosphate + Hydrogen ion + a-Kdo-(2->4)-a-Kdo-(2->6)-lipid IVA + Cytidine monophosphate + a-Kdo-(2->4)-a-Kdo-(2->6)-lipid IVA
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PseudoCyc/BioCyc Reactions:

lipid IV_A

↔

CMP-3-Deoxy-D-manno-octulosonate + lipid IV_A ↔ Hydrogen ion + KDO-lipid IV(A) + Cytidine monophosphate
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↔

α-Kdo-(2->4)-α-Kdo-(2->6)-lipid IV_A

KDO-lipid IV(A) + CMP-3-Deoxy-D-manno-octulosonate ↔ Hydrogen ion + α-Kdo-(2->4)-α-Kdo-(2->6)-lipid IV_A + Cytidine monophosphate
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Complex Reactions:

→

CMP-3-Deoxy-D-manno-octulosonate + 2,3,2'3'-Tetrakis(beta-hydroxymyristoyl)-D-glucosaminyl-1,6-beta-D-glucosamine 1,4'-bisphosphate → Cytidine monophosphate + Hydrogen ion + KDO-lipid IV(A)
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→

CMP-3-Deoxy-D-manno-octulosonate + KDO-lipid IV(A) → Cytidine monophosphate + Hydrogen ion + KDO(2)-lipid IV(A)
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→

alpha-Kdo-(2->6)-lipid IV(A)

2,3,2'3'-Tetrakis(beta-hydroxymyristoyl)-D-glucosaminyl-1,6-beta-D-glucosamine 1,4'-bisphosphate + CMP-3-Deoxy-D-manno-octulosonate → alpha-Kdo-(2->6)-lipid IV(A) + Cytidine monophosphate
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Alpha-Kdo-(2->6)-lipid IV(A)

→

alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-lipid IV(A)

Alpha-Kdo-(2->6)-lipid IV(A) + CMP-3-Deoxy-D-manno-octulosonate → alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-lipid IV(A) + Cytidine monophosphate
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Transports:

Not Available

Metabolites:

PAMDB ID	Name	View
PAMDB003481	(2-N,3-O-bis(3-Hydroxytetradecanoyl)-4-O-phosphono-beta-D-glucosaminyl)-(1->6)-(2-N,3-O-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl phosphate)	MetaboCard
PAMDB000906	2,3,2'3'-Tetrakis(3-hydroxytetradecanoyl)-D-glucosaminyl-1,6-beta-D-glucosamine 1,4'-bisphosphate	MetaboCard
PAMDB001545	2,3,2'3'-Tetrakis(beta-hydroxymyristoyl)-D-glucosaminyl-1,6-beta-D-glucosamine 1,4'-bisphosphate	MetaboCard
PAMDB006295	a-Kdo-(2->4)-a-Kdo-(2->6)-lipid IVA	MetaboCard
PAMDB006335	alpha-Kdo-(2??)-lipid IVA	MetaboCard
PAMDB000981	CMP-3-Deoxy-D-manno-octulosonate	MetaboCard
PAMDB000035	Cytidine monophosphate	MetaboCard
PAMDB001633	Hydrogen ion	MetaboCard
PAMDB001643	KDO(2)-lipid IV(A)	MetaboCard
PAMDB001644	KDO-lipid IV(A)	MetaboCard

GO Classification:

Function
binding
carbohydrate binding
catalytic activity
sugar binding
transferase activity
Process
biosynthetic process
carbohydrate metabolic process
metabolic process
primary metabolic process

Gene Properties

Locus tag:

PA4988

Strand:

Entrez Gene ID:

880145

Accession:

NP_253675.1

GI:

15600181

Sequence start:

5603821

Sequence End:

5605098

Sequence Length:

1277

Gene Sequence:

>PA4988
ATGAACCGTACCCTCTATACCCTGCTGTTCCACCTCGGCTTGCCGCTGGTGGCGTTGCGCCTGTGGTGGCGCGCGCGCCAGGCGCCGGCCTATGCCAAGCGCATCGGCGAGCGTTTTTCCCTGTCCTTGCCCGAGGTGCCGCCCGGCGGCATCTGGGTGCACGCCGTCTCGGTGGGCGAGAGCATCGCCGCCGCGCCGATGGTGCGGGCGCTGCTGGAGCGCCATCCGCAGCTGCCGGTCACCGTCACCTGCATGACCCCGACCGGCTCGGAGCGCATCCGCGCATTGTTCGGCGAGCAGGTCCGGCACTGCTACCTGCCCTACGACCTGCCCTGGGCGGCGGCGCGCTTCCTCGACCGGGTGCGGCCGCGCCTGGCGGTGATCATGGAAACCGAGCTGTGGCCCAACCATATCCATGCCTGCGCCGTGCGCGGGATCCCGGTGGCGCTGGCCAATGCGCGCCTGTCCGAGCGCTCGGCGCGTGGCTACGCGCGCTTCGCCGGGCTGACCCGGCCGATGCTCGCCGAGCTGTCCTGGATCGCCGTGCAGACCGAGGCCGAGGCCGAGCGCTTCCGTCGTCTCGGCGCGCGTCCTGAGTGCGTCAGCGTGACCGGCTCGATCAAGTTCGACCTGCGCATCGACCCGCAGCTGCCGCTGGCCGCTGCCGCCCTGCGCGAGGAATGGGACGCGACGGCGCGACCGCTGTGGATCGCCGCCAGCACCCATGCCGGCGAGGACGAGATCGTCCTGGCAGCCCACCGGCGCCTGCTGGAAACGCGTCCCGACGCGTTGCTGATCCTGGTGCCGCGCCATCCGGAACGCTTCGCCGGCGTCCACGAGCTATGCCGCCGCGAAGGCTTCGCCACGGTCCGGCGCTCCGGCGGCGAGCCGGTGGCACGCGCGACCCAGGTGCTGCTCGGCGATACCATGGGCGAGCTGCTGTTCCTCTATGCCCTGGCCGACATCGCTTTCGTCGGCGGCAGCCTGGTGCCCAACGGCGGGCACAACCTGCTGGAGCCGGCGGCCCTGGGCAAGCCGGTGTTCGCCGGTCCGCACCTGTTCAACTTCCTCGACATCGCCGCGCAATTGCGCGACGCCGGGGCGCTGCTCGAGGTGACGGATGCCGGCGAGCTGTGCGACGGCCTCGCGCGCCTGTGGGCGCAGCCGGAGGTCGCGACGGCCATGGCCACTGCCGGAGAGAAGGTGCTGCGCAACAACCAGGGCGCCCTGGAGCGGCTGCTGGCGGGGTTGGCGCGGTTGCTCGGGCGCGGCGGGTAG

Protein Properties

Protein Residues:

425

Protein Molecular Weight:

46.5 kDa

Protein Theoretical pI:

8.83

Hydropathicity (GRAVY score):

0.102

Charge at pH 7 (predicted):

7.72

Protein Sequence:

>PA4988
MNRTLYTLLFHLGLPLVALRLWWRARQAPAYAKRIGERFSLSLPEVPPGGIWVHAVSVGESIAAAPMVRALLERHPQLPVTVTCMTPTGSERIRALFGEQVRHCYLPYDLPWAAARFLDRVRPRLAVIMETELWPNHIHACAVRGIPVALANARLSERSARGYARFAGLTRPMLAELSWIAVQTEAEAERFRRLGARPECVSVTGSIKFDLRIDPQLPLAAAALREEWDATARPLWIAASTHAGEDEIVLAAHRRLLETRPDALLILVPRHPERFAGVHELCRREGFATVRRSGGEPVARATQVLLGDTMGELLFLYALADIAFVGGSLVPNGGHNLLEPAALGKPVFAGPHLFNFLDIAAQLRDAGALLEVTDAGELCDGLARLWAQPEVATAMATAGEKVLRNNQGALERLLAGLARLLGRGG

References

External Links:

Resource	Link
Genome ID:	PA4988
Entrez Gene ID:	880145
NCBI Protein ID:	15600181

General Reference:

PaperBLAST - Find papers about PA4988 and its homologs

3-deoxy-D-manno-octulosonic-acid transferase (PA4988)