Ketol-acid reductoisomerase (PA4694)

Identification
Name: Ketol-acid reductoisomerase
Synonyms:
  • Acetohydroxy-acid isomeroreductase
  • Alpha-keto-beta-hydroxylacil reductoisomerase
Gene Name: ilvC
Enzyme Class:
Biological Properties
General Function: Involved in oxidation-reduction process
Specific Function: (R)-2,3-dihydroxy-3-methylbutanoate + NADP(+) = (S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH
Cellular Location: Not Available
KEGG Pathways:
KEGG Reactions:
Thumb+Thumb+Thumb ↔ Thumb+Thumb
2-Dehydropantoate + Hydrogen ion + NADPH ↔ NADP + (R)-Pantoate
ReactionCard
Thumb+Thumb+Thumb ↔ Thumb+Thumb
2-Acetolactate + NADPH + Hydrogen ion ↔ 2,3-Dihydroxyisovaleric acid + NADP
ReactionCard
Thumb+Thumb ↔ Thumb+Thumb+Thumb
(R)-2,3-Dihydroxy-isovalerate + NADP ↔ 3-Hydroxy-3-methyl-2-oxobutanoic acid + NADPH + Hydrogen ion
ReactionCard
Thumb+Thumb ↔ Thumb+Thumb+Thumb
(R) 2,3-Dihydroxy-3-methylvalerate + NADP ↔ (R)-3-Hydroxy-3-methyl-2-oxopentanoate + NADPH + Hydrogen ion
ReactionCard
Thumb ↔ Thumb
2-Aceto-2-hydroxy-butyrate ↔ (R)-3-Hydroxy-3-methyl-2-oxopentanoate
ReactionCard
Thumb ↔ Thumb
(S)-2-Acetolactate ↔ 3-Hydroxy-3-methyl-2-oxobutanoic acid
ReactionCard
Thumb+Thumb ↔ Thumb+Thumb+Thumb
(R)-2,3-Dihydroxy-isovalerate + NADP ↔ (S)-2-Acetolactate + NADPH + Hydrogen ion
ReactionCard
SMPDB Reactions:
Thumb+Thumb+NADPH+Thumb → Thumb+Thumb
(R)-2,3-Dihydroxy-isovalerate + Hydrogen ion + NADPH + NADPH → NADP + (R) 2,3-Dihydroxy-3-methylvalerate
ReactionCard
Thumb+NADPH+Thumb+Thumb → Thumb+Thumb
2-Aceto-2-hydroxy-butyrate + NADPH + Hydrogen ion + NADPH → NADP + (R) 2,3-Dihydroxy-3-methylvalerate
ReactionCard
2-dehydropantoate+NADPH+Thumb+Thumb+Thumb → Thumb+(R)-pantoate+Thumb
2-dehydropantoate + NADPH + Hydrogen ion + 2-Dehydropantoate + NADPH → NADP + (R)-pantoate + (R)-Pantoate
ReactionCard
Thumb+Thumb+NADPH+Thumb → Thumb+Thumb
(S)-2-Acetolactate + Hydrogen ion + NADPH + NADPH → NADP + (R)-2,3-Dihydroxy-isovalerate
ReactionCard
Thumb ↔ Thumb
(S)-2-Acetolactate ↔ 3-Hydroxy-3-methyl-2-oxobutanoic acid
ReactionCard
PseudoCyc/BioCyc Reactions:
Thumb+Thumb+Thumb ↔ Thumb+Thumb
2-Dehydropantoate + Hydrogen ion + NADPH ↔ NADP + (R)-Pantoate
ReactionCard
Thumb+Thumb+Thumb ↔ Thumb+Thumb
2-Aceto-2-hydroxy-butyrate + Hydrogen ion + NADPH ↔ (R) 2,3-Dihydroxy-3-methylvalerate + NADP
ReactionCard
Complex Reactions:
Thumb+Thumb ↔ Thumb+Thumb+Thumb
(R)-2,3-Dihydroxy-isovalerate + NADP ↔ (S)-2-Acetolactate + Hydrogen ion + NADPH
ReactionCard
Thumb+Thumb → Thumb+Thumb
(R)-2,3-Dihydroxy-isovalerate + NADP → (S)-2-Acetolactate + NADPH
ReactionCard
Thumb+Thumb → Thumb+Thumb
(R) 2,3-Dihydroxy-3-methylvalerate + NADP → 2-Aceto-2-hydroxy-butyrate + NADPH
ReactionCard
Transports: Not Available
Metabolites:
PAMDB IDNameView
PAMDB000822(R) 2,3-Dihydroxy-3-methylvalerateMetaboCard
PAMDB000589(R)-2,3-Dihydroxy-isovalerateMetaboCard
PAMDB000863(R)-3-Hydroxy-3-methyl-2-oxopentanoateMetaboCard
PAMDB000865(R)-PantoateMetaboCard
PAMDB000697(S)-2-AcetolactateMetaboCard
PAMDB0018752,3-Dihydroxyisovaleric acidMetaboCard
PAMDB0005492-Aceto-2-hydroxy-butyrateMetaboCard
PAMDB0005502-AcetolactateMetaboCard
PAMDB0005762-DehydropantoateMetaboCard
PAMDB0009193-Hydroxy-3-methyl-2-oxobutanoic acidMetaboCard
PAMDB001633Hydrogen ionMetaboCard
PAMDB000089NADPMetaboCard
PAMDB000615NADPHMetaboCard
GO Classification:
Function
binding
catalytic activity
ketol-acid reductoisomerase activity
NADP or NADPH binding
nucleotide binding
oxidoreductase activity
oxidoreductase activity, acting on CH-OH group of donors
oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
Process
branched chain family amino acid biosynthetic process
cellular amino acid and derivative metabolic process
cellular amino acid biosynthetic process
cellular amino acid metabolic process
cellular metabolic process
metabolic process
oxidation reduction
Gene Properties
Locus tag: PA4694
Strand: -
Entrez Gene ID: 881483
Accession: NP_253382.1
GI: 15599888
Sequence start: 5272455
Sequence End: 5273471
Sequence Length: 1016
Gene Sequence: 
>PA4694
ATGCGCGTTTTCTACGATAAAGACTGTGACCTCTCGATCATCCAGGGCAAGAAAGTTGCCATCATCGGCTACGGCTCCCAGGGCCACGCCCATGCCTGCAACCTGAAGGACTCCGGCGTCGACGTCACCGTGGGCCTGCGTAGCGGCTCCGCCACCGTGGCCAAGGCCGAAGCGCACGGTCTGAAGGTTGCCGACGTGAAGACCGCCGTCGCCGCAGCCGACGTGGTCATGATCCTCACCCCGGACGAGTTCCAGGGCCGCCTGTACAAGGAAGAGATCGAGCCGAACCTGAAGAAGGGCGCCACCCTGGCCTTCGCTCACGGCTTCTCCATCCACTACAACCAGGTCGTCCCGCGCGCCGACCTCGACGTGATCATGATCGCGCCGAAGGCACCGGGTCACACCGTGCGTTCCGAGTTCGTCAAGGGCGGTGGCATCCCTGACCTGATCGCCATCTACCAGGACGCTTCCGGCAACGCCAAGAACGTCGCCCTGTCCTACGCCTGCGGCGTCGGCGGCGGTCGTACCGGTATCATCGAAACCACCTTCAAGGACGAGACCGAAACCGACCTGTTCGGTGAGCAGGCCGTTCTCTGCGGTGGTTGCGTCGAGCTGGTCAAGGCCGGTTTCGAAACCCTGGTCGAAGCCGGTTACGCGCCGGAAATGGCCTACTTCGAGTGCCTGCACGAGCTGAAGCTGATCGTCGACCTGATGTACGAAGGCGGCATCGCCAACATGAACTACTCCATCTCCAACAATGCCGAATACGGTGAGTACGTAACCGGTCCGGAGGTGATCAACGCCGAGTCCCGTGCTGCCATGCGCAACGCCCTGAAGCGCATCCAGGACGGCGAGTACGCGAAAATGTTCATTACCGAAGGTGCGGCCAACTACCCGTCGATGACTGCCTACCGCCGCAACAACGCCGCTCACCCGATCGAGCAGATCGGCGAGAAGCTGCGCGCGATGATGCCGTGGATCGCAGCCAACAAGATCGTCGACAAGAGCAAGAACTAA
Protein Properties
Protein Residues: 338
Protein Molecular Weight: 36.4 kDa
Protein Theoretical pI: 5.65
Hydropathicity (GRAVY score): -0.092
Charge at pH 7 (predicted): -6.26
Protein Sequence: 
>PA4694
MRVFYDKDCDLSIIQGKKVAIIGYGSQGHAHACNLKDSGVDVTVGLRSGSATVAKAEAHGLKVADVKTAVAAADVVMILTPDEFQGRLYKEEIEPNLKKGATLAFAHGFSIHYNQVVPRADLDVIMIAPKAPGHTVRSEFVKGGGIPDLIAIYQDASGNAKNVALSYACGVGGGRTGIIETTFKDETETDLFGEQAVLCGGCVELVKAGFETLVEAGYAPEMAYFECLHELKLIVDLMYEGGIANMNYSISNNAEYGEYVTGPEVINAESRAAMRNALKRIQDGEYAKMFITEGAANYPSMTAYRRNNAAHPIEQIGEKLRAMMPWIAANKIVDKSKN
References
External Links:
Resource Link
Genome ID: PA4694
Entrez Gene ID: 881483
NCBI Protein ID: 15599888
General Reference: PaperBLAST - Find papers about PA4694 and its homologs