UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase (PA4417)

Identification
Name: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase
Synonyms:
  • Meso-A2pm-adding enzyme
  • Meso-diaminopimelate-adding enzyme
  • UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase
  • UDP-MurNAc-tripeptide synthetase
  • UDP-N-acetylmuramyl-tripeptide synthetase
Gene Name: murE
Enzyme Class:
Biological Properties
General Function: Involved in ATP binding
Specific Function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan. Is also able to use many meso-diaminopimelate analogs as substrates, although much less efficiently, but not L-lysine
Cellular Location: Cytoplasm (Probable)
KEGG Pathways:
KEGG Reactions:
Thumb+Thumb+Thumb ↔ Thumb+Thumb+Thumb
Adenosine triphosphate + UDP-N-Acetylmuramoyl-L-alanyl-D-glutamate + Meso-2,6-Diaminoheptanedioate ↔ ADP + Phosphate + UDP-N-Acetylmuramoyl-L-alanyl-D-glutamyl-meso-2,6-diaminoheptanedioate
ReactionCard
SMPDB Reactions:
UDP-N-acetylmuramoyl-L-alanyl-D-glutamate+Thumb+Thumb+Thumb → Adenosine diphosphate+Thumb+Thumb+Thumb+Thumb
UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + Meso-2,6-Diaminoheptanedioate + Adenosine triphosphate + UDP-N-Acetylmuramoyl-L-alanyl-D-glutamate → Adenosine diphosphate + Phosphate + Hydrogen ion + UDP-N-Acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diaminopimelate + ADP
ReactionCard
PseudoCyc/BioCyc Reactions:
meso-diaminopimelate+Thumb+Thumb → Thumb+Thumb+Thumb+Thumb
meso-diaminopimelate + UDP-N-Acetylmuramoyl-L-alanyl-D-glutamate + Adenosine triphosphate → Hydrogen ion + UDP-N-Acetylmuramoyl-L-alanyl-D-glutamyl-meso-2,6-diaminoheptanedioate + Phosphate + ADP
ReactionCard
Complex Reactions:
Thumb+Thumb+Thumb → Thumb+Thumb+Thumb+Thumb
Diaminopimelic acid + Adenosine triphosphate + UDP-N-Acetylmuramoyl-L-alanyl-D-glutamate → ADP + Hydrogen ion + Phosphate + UDP-N-Acetylmuramoyl-L-alanyl-D-glutamyl-meso-2,6-diaminoheptanedioate
ReactionCard
Transports: Not Available
Metabolites:
PAMDB IDNameView
PAMDB000148Adenosine triphosphateMetaboCard
PAMDB000345ADPMetaboCard
PAMDB000360Diaminopimelic acidMetaboCard
PAMDB001633Hydrogen ionMetaboCard
PAMDB001776Inorganic phosphateMetaboCard
PAMDB002055Meso-2,6-DiaminoheptanedioateMetaboCard
PAMDB000383PhosphateMetaboCard
PAMDB001049UDP-N-Acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diaminopimelateMetaboCard
PAMDB000649UDP-N-Acetylmuramoyl-L-alanyl-D-glutamateMetaboCard
PAMDB000650UDP-N-Acetylmuramoyl-L-alanyl-D-glutamyl-meso-2,6-diaminoheptanedioateMetaboCard
GO Classification:
Component
cell part
cytoplasm
intracellular part
Function
acid-amino acid ligase activity
adenyl nucleotide binding
adenyl ribonucleotide binding
ATP binding
binding
catalytic activity
ligase activity
ligase activity, forming carbon-nitrogen bonds
nucleoside binding
purine nucleoside binding
Process
aminoglycan metabolic process
biological regulation
biosynthetic process
carbohydrate metabolic process
cell division
cell wall biogenesis
cell wall organization or biogenesis
cellular cell wall organization or biogenesis
cellular component organization or biogenesis
cellular process
glycosaminoglycan metabolic process
metabolic process
peptidoglycan biosynthetic process
peptidoglycan metabolic process
peptidoglycan-based cell wall biogenesis
polysaccharide metabolic process
primary metabolic process
regulation of biological quality
regulation of cell shape
Gene Properties
Locus tag: PA4417
Strand: -
Entrez Gene ID: 881246
Accession: NP_253107.1
GI: 15599613
Sequence start: 4951141
Sequence End: 4952604
Sequence Length: 1463
Gene Sequence: 
>PA4417
ATGCCTATGAGCCTGAACCAACTGTTTCCCCAGGCCGAGCGCGATCTGCTGATCCGCGAGCTGACCCTGGATAGCCGCGGCGTTCGTCCGGGCGACCTGTTCCTGGCGGTGCCGGGCGGGCGCCAGGATGGTCGTGCGCACATCGCCGATGCCCTGGCCAAGGGCGCGGCTGCCGTGGCTTACGAGGCGGAAGGCGCCGGAGAGTTGCCGCCCAGCGATGCGCCGCTGATCGCGGTGAAGGGGCTGGCCGCGCAACTGTCGGCGGTCGCCGGGCGTTTCTACGGCGAGCCGAGCCGCGGGCTGGACCTGATCGGCGTCACCGGCACCAACGGCAAGACCAGCGTCAGCCAACTGGTGGCCCAGGCCCTGGATCTGCTCGGCGAGCGCTGCGGCATCGTCGGCACCCTCGGCACCGGTTTCTACGGCGCCCTGGAGAGCGGCCGGCACACCACGCCGGACCCGCTCGCGGTGCAGGCCACGCTGGCCACGCTGAAGCAGGCCGGCGCCCGCGCGGTAGCGATGGAAGTGTCTTCCCACGGCCTCGACCAGGGCCGCGTGGCGGCGCTCGGTTTCGATATCGCGGTGTTCACCAATCTGTCCCGCGACCACCTCGACTATCACGGTTCGATGGAAGCCTATGCCGCCGCCAAGGCCAAGCTGTTCGCCTGGCCGGGCCTGCGCTGCCGGGTGATCAACCTGGACGACGATTTCGGCCGTCGACTGGCCGGCGAGGAGCAGGACTCGGAGCTGATCACCTACAGCCTCACCGACAGCTCGGCGTTCCTCTATTGCCGCGAAGCGCGCTTCGGCGACGCCGGCATCGAGGCGGCGCTGGTCACTCCGCACGGCGAGGGCCTGCTGCGCAGCCCGTTGCTCGGCCGCTTCAACCTGAGCAACCTGCTGGCGGCGGTCGGTGCGTTGCTTGGCCTGGGTTATCCCCTGGGCGATATCCTCCGCACTTTGCCGCAACTGCAGGGGCCGGTCGGCCGCATGCAGCGCCTGGGAGGCGGCGACAAGCCGCTGGTGGTGGTGGACTACGCGCATACTCCCGACGCCCTGGAAAAAGTCCTGGAGGCCCTGCGTCCGCACGCGGCCGCGCGCCTGCTGTGCCTGTTCGGCTGCGGTGGCGATCGCGATGCCGGCAAGCGTCCGCTGATGGCTGCGATCGCCGAACGCCTGGCGGATGAGGTGCTGGTCACCGACGACAACCCGCGCACCGAGGCCAGTGCGGCGATCATCGCCGATATCCGCAAAGGCTTCGCTGCCGCTGACAAGGTTACCTTCCTGCCGTCGCGCGGTGAGGCGATCGCCCATCTGATCGCTTCCGCTGCGGTGGATGACGTGGTGCTCCTGGCCGGCAAGGGTCACGAGGATTATCAGGAGATCGACGGCGTACGCCATCCGTTCTCCGACATCGAGCAGGCCGAGCGCGCCCTGGCCGCCTGGGAGGTGCCGCATGCTTGA
Protein Properties
Protein Residues: 487
Protein Molecular Weight: 51.3 kDa
Protein Theoretical pI: 5.11
Hydropathicity (GRAVY score): 0.044
Charge at pH 7 (predicted): -13.24
Protein Sequence: 
>PA4417
MPMSLNQLFPQAERDLLIRELTLDSRGVRPGDLFLAVPGGRQDGRAHIADALAKGAAAVAYEAEGAGELPPSDAPLIAVKGLAAQLSAVAGRFYGEPSRGLDLIGVTGTNGKTSVSQLVAQALDLLGERCGIVGTLGTGFYGALESGRHTTPDPLAVQATLATLKQAGARAVAMEVSSHGLDQGRVAALGFDIAVFTNLSRDHLDYHGSMEAYAAAKAKLFAWPGLRCRVINLDDDFGRRLAGEEQDSELITYSLTDSSAFLYCREARFGDAGIEAALVTPHGEGLLRSPLLGRFNLSNLLAAVGALLGLGYPLGDILRTLPQLQGPVGRMQRLGGGDKPLVVVDYAHTPDALEKVLEALRPHAAARLLCLFGCGGDRDAGKRPLMAAIAERLADEVLVTDDNPRTEASAAIIADIRKGFAAADKVTFLPSRGEAIAHLIASAAVDDVVLLAGKGHEDYQEIDGVRHPFSDIEQAERALAAWEVPHA
References
External Links:
Resource Link
Genome ID: PA4417
Entrez Gene ID: 881246
NCBI Protein ID: 15599613
General Reference: PaperBLAST - Find papers about PA4417 and its homologs