| Identification |
| Name: |
Glyoxylate/hydroxypyruvate reductase B |
| Synonyms: |
- 2-ketoaldonate reductase
- 2-ketogluconate reductase
- 2KR
|
| Gene Name: |
ghrB |
| Enzyme Class: |
|
| Biological Properties |
| General Function: |
Involved in oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor |
| Specific Function: |
Catalyzes the NADPH-dependent reduction of glyoxylate and hydroxypyruvate into glycolate and glycerate, respectively. Can also reduce 2,5-diketo-D-gluconate (25DKG) to 5-keto-D- gluconate (5KDG), 2-keto-D-gluconate (2KDG) to D-gluconate, and 2- keto-L-gulonate (2KLG) to L-idonate (IA), but it is not its physiological function. Inactive towards 2-oxoglutarate, oxaloacetate, pyruvate, 5-keto-D-gluconate, D-fructose and L- sorbose. Activity with NAD is very low |
| Cellular Location: |
Cytoplasm (Probable) |
| KEGG Pathways: |
|
| KEGG Reactions: |
| | | | | | |
 | + |  | ↔ | 2-Keto-D-gluconic acid | + |  | + |  | + |  |
| |
|
| SMPDB Reactions: |
|
 | + | NADPH | + | | + | | → | | + | 5-Keto-D-gluconate | + |  |
| | |
2-Keto-L-gluconate | + | | + | NADPH | + | | + | | → | | + |  |
| |
|
| PseudoCyc/BioCyc Reactions: |
|
| Complex Reactions: |
| | |
2 -Dehydro-L-gulonate | + | | + |  | → | | + |  |
| | |
2 -Dehydro-L-gulonate | + | | + | | → |  | + | |
| | |
2 -Dehydro-L-gulonate | + | | + | | → | | + | |
| | |
2 -Dehydro-L-gulonate | + | | + | | → | | + | |
| | | | | | |
 | + | NAD(P)(+) | → |  | + | NAD(P)H |
| |
|
| Transports: |
Not Available |
| Metabolites: |
|
| GO Classification: |
| Function |
|---|
| binding | | catalytic activity | | cofactor binding | | NAD or NADH binding | | nucleotide binding | | oxidoreductase activity | | oxidoreductase activity, acting on CH-OH group of donors | | oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor | | Process |
|---|
| metabolic process |
|
| Gene Properties |
| Locus tag: |
PA2263 |
| Strand: |
+ |
| Entrez Gene ID: |
877698 |
| Accession: |
NP_250953.1 |
| GI: |
15597459 |
| Sequence start: |
2492064 |
| Sequence End: |
2493050 |
| Sequence Length: |
986 |
| Gene Sequence: |
>PA2263
ATGAAGAAAGTGGTTCTCTACAAACGTCTCTCCGCGCCCCTGATGGAACGCCTGCGCGAACGGGTCGAGGTGCTCCTGGTCGAGGAGCCCGGCCGCGACGGCCTGGCCCGCCTGCGCGACGCCCTGCCCGAGGCCCACGGCCTGCTCGGCGCCAGCCTGCGCCTGGACGCCGGACTGCTCGACCTGGCACCGCGACTGGAAGCCATCGCCAGCGTCTCGGTGGGCGTCGACAACTATGACATCGACTACCTCGACCGCCGCGGCATCCTCCTCAGCAATACCCCCGACGTGCTCACCGAGACCACCGCCGACACCGGCTTCGCGCTGATCCTGGCGACAGCCCGGCGGGTAGTGGAACTGGCCGGCTGGGTGCGCGCCGGGGAATGGAAGAAGAGCGTCGGCGCCGCCCAGTTCGGTACCGACGTGCATGGCAAGACCCTCGGTATCGTCGGCATGGGCCGCATCGGCGAGGCCCTGGCCCGGCGCGGGCACCACGGCTTCGGCATGCGCGTGCTGTACCACAGCCATAGCCCGAAGCCCCACGTCGAAGAACGCTACGCAGCGAGCTACCGCCCCCTCGACGCGCTGCTCGAGGAATCCGACTTCGTCTGCCTGACCCTGCCGCTGACCGCCGCCACCGAAGGCCTGATCGGTGCCGCGCAGTTCGCCCGGATGCGCCCGCAGGCGATCTTCATCAACATCTCCCGTGGCCGGGTGGTCGACGAGGCGGCCCTGATCGAGGCCCTGGCCCAGCGGCGGATCCGCGCCGCCGGCCTCGACGTGTTCGAGCGCGAGCCGCTGTCTCCCGACTCGCCGCTGCTGCGCCTGCCGAACGTGGTGGCCACTCCCCACATCGGCTCGGCCACCGAGGAAACCCGCGAAGCCATGGCCCGCTGCGCGGTAGACAACCTGCTGGCCGCCCTCGCCGGCGCGCGGCCGCTGAACCTGGTCAATCCTTCGGCCTGGGCGCGACGGCGAGGCGCCTGA |
| Protein Properties |
| Protein Residues: |
328 |
| Protein Molecular Weight: |
35.6 kDa |
| Protein Theoretical pI: |
8.69 |
| Hydropathicity (GRAVY score): |
-0.006 |
| Charge at pH 7 (predicted): |
3.87 |
| Protein Sequence: |
>PA2263
MKKVVLYKRLSAPLMERLRERVEVLLVEEPGRDGLARLRDALPEAHGLLGASLRLDAGLLDLAPRLEAIASVSVGVDNYDIDYLDRRGILLSNTPDVLTETTADTGFALILATARRVVELAGWVRAGEWKKSVGAAQFGTDVHGKTLGIVGMGRIGEALARRGHHGFGMRVLYHSHSPKPHVEERYAASYRPLDALLEESDFVCLTLPLTAATEGLIGAAQFARMRPQAIFINISRGRVVDEAALIEALAQRRIRAAGLDVFEREPLSPDSPLLRLPNVVATPHIGSATEETREAMARCAVDNLLAALAGARPLNLVNPSAWARRRGA |
| References |
| External Links: |
|
| General Reference: |
PaperBLAST - Find papers about PA2263 and its homologs
|
