Dihydrofolate reductase (PA0350)

Identification
Name: Dihydrofolate reductase
Synonyms: Not Available
Gene Name: folA
Enzyme Class:
Biological Properties
General Function: Involved in dihydrofolate reductase activity
Specific Function: Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis
Cellular Location: Not Available
KEGG Pathways:
KEGG Reactions:
Thumb+Thumb+Thumb ↔ Thumb+Thumb
Dihydrofolic acid + Hydrogen ion + NADPH ↔ NADP + Tetrahydrofolic acid
ReactionCard
Thumb+Thumb ↔ Thumb+Thumb+Thumb
Tetrahydrofolic acid + NAD ↔ Dihydrofolic acid + NADH + Hydrogen ion
ReactionCard
Thumb+2 Thumb ↔ Thumb+2 Thumb+2 Thumb
Tetrahydrofolic acid + 2 NAD ↔ Folic acid + 2 NADH + 2 Hydrogen ion
ReactionCard
Thumb+Thumb ↔ Thumb+Thumb+Thumb
Tetrahydrofolic acid + NADP ↔ Dihydrofolic acid + NADPH + Hydrogen ion
ReactionCard
Thumb+2 Thumb ↔ Thumb+2 Thumb+2 Thumb
Tetrahydrofolic acid + 2 NADP ↔ Folic acid + 2 NADPH + 2 Hydrogen ion
ReactionCard
Thumb+Thumb ↔ Thumb+Thumb+Thumb
Dihydrofolic acid + NAD ↔ Folic acid + NADH + Hydrogen ion
ReactionCard
Thumb+Thumb ↔ Thumb+Thumb+Thumb
Dihydrofolic acid + NADP ↔ Folic acid + NADPH + Hydrogen ion
ReactionCard
SMPDB Reactions:
Thumb+Thumb+Dihydrofolic acid → Thumb+NADPH+Thumb+Thumb
Dihydrofolic acid + NADP + Dihydrofolic acid → Folic acid + NADPH + Hydrogen ion + NADPH
ReactionCard
Thumb+NADPH+Thumb+Dihydrofolic acid+Thumb → Tetrahydrofolic acid+Thumb+Thumb
Dihydrofolic acid + NADPH + Hydrogen ion + Dihydrofolic acid + NADPH → Tetrahydrofolic acid + NADP + Tetrahydrofolic acid
ReactionCard
7,8-dihydrofolate monoglutamate+Thumb+NADPH+Thumb+Thumb → Thumb+Tetrahydrofolic acid+Thumb
7,8-dihydrofolate monoglutamate + Hydrogen ion + NADPH + Dihydrofolic acid + NADPH → NADP + Tetrahydrofolic acid + Tetrahydrofolic acid
ReactionCard
Thumb+2 NADPH+2 Thumb+2 Thumb → Tetrahydrofolic acid+2 Thumb+Thumb
Folic acid + 2 NADPH + 2 Hydrogen ion + 2 NADPH → Tetrahydrofolic acid + 2 NADP + Tetrahydrofolic acid
ReactionCard
PseudoCyc/BioCyc Reactions:
Thumb+Thumb+Thumb ↔ Thumb+Thumb
Dihydrofolic acid + Hydrogen ion + NADPH ↔ NADP + Tetrahydrofolic acid
ReactionCard
Thumb+Thumb ← Thumb+Thumb+Thumb
NADP + Tetrahydrofolic acid ← Hydrogen ion + NADPH + Dihydrofolic acid
ReactionCard
Complex Reactions:
Thumb+Thumb → Thumb+Thumb
Tetrahydrofolic acid + NADP → Dihydrofolic acid + NADPH
ReactionCard
Transports: Not Available
Metabolites:
PAMDB IDNameView
PAMDB000235Dihydrofolic acidMetaboCard
PAMDB000044Folic acidMetaboCard
PAMDB001633Hydrogen ionMetaboCard
PAMDB000197NADMetaboCard
PAMDB000398NADHMetaboCard
PAMDB000089NADPMetaboCard
PAMDB000615NADPHMetaboCard
PAMDB000424Tetrahydrofolic acidMetaboCard
GO Classification:
Function
binding
catalytic activity
dihydrofolate reductase activity
NADP or NADPH binding
nucleotide binding
oxidoreductase activity
oxidoreductase activity, acting on the CH-NH group of donors
oxidoreductase activity, acting on the CH-NH group of donors, NAD or NADP as acceptor
Process
cellular amino acid and derivative metabolic process
cellular amino acid metabolic process
cellular metabolic process
cellular nitrogen compound metabolic process
glycine biosynthetic process
glycine metabolic process
metabolic process
nitrogen compound metabolic process
nucleobase, nucleoside and nucleotide metabolic process
nucleobase, nucleoside, nucleotide and nucleic acid metabolic process
nucleoside phosphate metabolic process
nucleotide biosynthetic process
nucleotide metabolic process
oxidation reduction
serine family amino acid metabolic process
Gene Properties
Locus tag: PA0350
Strand: +
Entrez Gene ID: 879233
Accession: NP_249041.1
GI: 15595547
Sequence start: 393308
Sequence End: 393814
Sequence Length: 506
Gene Sequence: 
>PA0350
ATGGCACGACCGCTGGCGATGATCGCCGCCCTTGGCGAGAACCGCGCGATCGGCATCGACAACCGCCTGCCCTGGCGCCTGCCGGCCGACCTCAAGCATTTCAAGGCGATGACCCTCGGCAAGCCGGTGATCATGGGGCGCAAGACCTGGGACTCGCTGGGTCGGCCGCTGCCGGGCCGGCTAAACCTGGTGGTCAGCCGCCAGGCCGGCCTGGCACTGGAGGGTGCGGAAGTGTTCGCCAGCCTGGACGCCGCGCTGGCGCGCGCCGAGGCGTGGGCGCAGGCGGAGGACGCCGACGAACTGATGCTGATCGGCGGCGCCCAGCTCTATGCCGAGGCCTTGCCGCGCGCCGCGCGTCTCTACCTGACCCGGGTTGGCCTGGCGCCGGAGGGCGACGCCTTTTTCCCCGAGATCGATGGTGCGGCCTGGCGCCTGGCGTCGAGCATCGAACACGCCGCGGCTGACGATGCGCCTGCCTACGCCTTCGAGGTATGGGAGCGCCGCTGA
Protein Properties
Protein Residues: 168
Protein Molecular Weight: 18.2 kDa
Protein Theoretical pI: 5.3
Hydropathicity (GRAVY score): 0.021
Charge at pH 7 (predicted): -2.52
Protein Sequence: 
>PA0350
MARPLAMIAALGENRAIGIDNRLPWRLPADLKHFKAMTLGKPVIMGRKTWDSLGRPLPGRLNLVVSRQAGLALEGAEVFASLDAALARAEAWAQAEDADELMLIGGAQLYAEALPRAARLYLTRVGLAPEGDAFFPEIDGAAWRLASSIEHAAADDAPAYAFEVWERR
References
External Links:
Resource Link
Genome ID: PA0350
Entrez Gene ID: 879233
NCBI Protein ID: 15595547
General Reference: PaperBLAST - Find papers about PA0350 and its homologs