Glutamate synthase [NADPH] large chain (PA5036)

Identification
Name: Glutamate synthase [NADPH] large chain
Synonyms:
  • Glutamate synthase subunit alpha
  • GLTS alpha chain
  • NADPH-GOGAT
Gene Name: gltB
Enzyme Class:
Biological Properties
General Function: Involved in catalytic activity
Specific Function: 2 L-glutamate + NADP(+) = L-glutamine + 2- oxoglutarate + NADPH
Cellular Location: Not Available
KEGG Pathways:
KEGG Reactions:
Thumb+Thumb+Thumb+Thumb → 2 Thumb+Thumb
alpha-Ketoglutarate + L-Glutamine + Hydrogen ion + NADPH → 2 L-Glutamate + NADP
ReactionCard
2 Thumb+Thumb ↔ Thumb+Thumb+Thumb+Thumb
2 L-Glutamate + NAD ↔ L-Glutamine + alpha-Ketoglutarate + NADH + Hydrogen ion
ReactionCard
2 Thumb+Thumb ↔ Thumb+Thumb+Thumb+Thumb
2 L-Glutamate + NADP ↔ L-Glutamine + alpha-Ketoglutarate + NADPH + Hydrogen ion
ReactionCard
2 Thumb+Thumb+Thumb+Thumb ↔ Thumb+Thumb+Thumb+Thumb
2 L-Glutamate + NADP + Ammonia + Water ↔ L-Glutamine + alpha-Ketoglutarate + NADPH + Hydrogen ion
ReactionCard
SMPDB Reactions:
2 L-Glutamic acid+Thumb+2 Thumb → Thumb+NADPH+Thumb+Thumb+Thumb
2 L-Glutamic acid + NADP + 2 L-Glutamate → L-Glutamine + NADPH + Hydrogen ion + Oxoglutaric acid + NADPH
ReactionCard
Thumb+Thumb+NADPH+Thumb+Thumb → 2 L-Glutamic acid+Thumb+2 Thumb
L-Glutamine + Oxoglutaric acid + NADPH + Hydrogen ion + NADPH → 2 L-Glutamic acid + NADP + 2 L-Glutamate
ReactionCard
PseudoCyc/BioCyc Reactions:
2 Thumb+Thumb → Thumb+Thumb+Thumb
2 L-Glutamate + NADP → L-Glutamine + Oxoglutaric acid + NADPH
ReactionCard
2 Thumb+Thumb → Thumb+Thumb+Thumb
2 L-Glutamate + NADP → L-Glutamine + Oxoglutaric acid + NADPH
ReactionCard
Complex Reactions: Not Available
Transports: Not Available
Metabolites:
PAMDB IDNameView
PAMDB000466alpha-KetoglutarateMetaboCard
PAMDB000017AmmoniaMetaboCard
PAMDB001633Hydrogen ionMetaboCard
PAMDB000055L-GlutamateMetaboCard
PAMDB000163L-GlutamineMetaboCard
PAMDB000197NADMetaboCard
PAMDB000398NADHMetaboCard
PAMDB000089NADPMetaboCard
PAMDB000615NADPHMetaboCard
PAMDB000623Oxoglutaric acidMetaboCard
PAMDB000142WaterMetaboCard
GO Classification:
Function
catalytic activity
glutamate synthase activity
oxidoreductase activity
oxidoreductase activity, acting on the CH-NH2 group of donors
Process
cellular amino acid and derivative metabolic process
cellular amino acid metabolic process
cellular metabolic process
glutamate biosynthetic process
glutamate metabolic process
glutamine family amino acid metabolic process
metabolic process
nitrogen compound metabolic process
oxidation reduction
Gene Properties
Locus tag: PA5036
Strand: -
Entrez Gene ID: 881222
Accession: NP_253723.1
GI: 15600229
Sequence start: 5667696
Sequence End: 5672141
Sequence Length: 4445
Gene Sequence: 
>PA5036
ATGAAAGCAGGTCTGTACCATCCTGAAACGTTCAAGGATAACTGCGGATTTGGCCTGATCGCCCATATGCAGGGCGAGCCGAGCCATCAACTTCTGCAAACCGCAATCGAAGCACTGACCTGCATGACCCACCGCGGTGGGATCAATGCCGATGGCAAGACCGGGGACGGCTGCGGGCTGCTGATCCAGAAGCCCGACCTGTTCCTGCGTGCCGTCGCCAGGGAAGCCTTTTCTGTCGACCTGCCCGAGCAATACGCGGTGGGCATGGTGTTCTTCAACCAGGACCCGGTGAAAGCCGAGGCGGCACGCGAGAACATGAATCGCGAGATCGTCGCCGCCGGACTGGAGCTGGTCGGCTGGCGCAAGGTGCCGATCGATACCAGCGTTCTCGGCCGCCTGGCGCTGGAGCGCCTGCCGCAGATCGAACAGGTGTTCATCGGCGGCGCCGGCCTGAGCGACCAGGACTTCGCCATCAAGCTGTTCAGCGCACGGCGCCGCTCGTCGGTGGCCAACGCCGCCGACAGCGACCACTACATCTGCAGCTTTTCGCACAAGACCATCATCTACAAGGGCCTGATGATGCCGGCCGACCTGGCCGCCTTCTATCCGGACCTTGGCGACGAGCGCCTGCAGACCGCGATCTGCGTATTCCACCAGCGCTTCTCCACCAACACCCTGCCGAAATGGCCGCTGGCGCAGCCGTTCCGCTTCCTCGCCCACAACGGCGAGATCAACACCATCACCGGCAACCGCAACTGGGCCCAGGCGCGGCGGACCAAGTTCACCAACGAACTGATCCCCGACCTGGAAGAGCTCGGCCCGCTGGTGAACCGCGTCGGCTCCGACTCGTCGAGCATGGACAACATGCTCGAACTGATGGTCACCGGCGGCATGGACCTGTTCCGCGGCCTGCGCATGATCATCCCGCCGGCCTGGCAGAACGTCGAAACCATGGACGCCGACCTGCGCGCGTTCTACGAGTACAACTCGCTGCACATGGAGCCCTGGGATGGCCCCGCCGGCGTCGTGCTGACCGACGGTCGCTATGCCGTCTGCCTGCTCGACCGCAATGGCCTGCGCCCGTCGCGCTGGGTCACCACGAAGAACGGCTACATCACCCTGGCTTCGGAAATCGGCGTCTGGGACTACAAGCCCGAGGACGTCATCGCCAAGGGTCGCGTCGGCCCGGGGCAGATCCTCGCGGTGGACACCGAGACCGGCCAGGTCCTGCACAGCGACGACATCGACAACCGCCTGAAGTCGCGCCATCCCTACAAGCAGTGGCTGCGGCAGAATGCCCTGCGCATCCAGGCGACCCTGGACGACGACCATGGCGTGGCCAGCTACGACGCCGACCAGCTCAAGCAATTCATGAAGATGTTCCAGGTCACCTTCGAGGAGCGTGACCAGGTGCTGCGTCCGCTCGGCGAGCAGGGCCAGGAAGCGGTGGGCTCCATGGGCGACGATACGCCGATGGCGATCCTCTCCCGGCGCATCCGCTCTCCGTACGATTACTTCCGCCAGCAATTCGCCCAGGTCACCAACCCGCCGATCGACCCGCTGCGCGAAGCCATCGTGATGTCCCTGGAAACCTGCCTGGGCGTCGAGCAGAACATCTTCGAGGAAGCCCCGCACCATGCCAACCAGGCGATCCTCACCACTCCGGTGATCTCGCCGGCGAAGTGGCGGACCATCATGACCCTGGACCGCCCGGGCTTCGACCGCCACTTCATCGACCTCAACTACGACGAGTCGATGGGCCTGGAGGCCGCGGTGCGCAACATCGCCGACCAGGCCGAGGAGGCCGTGCGCGGCGGCAAGGTGCTGCTGATCCTCAGCGACCGCCATATCGCTCCCGGCAAACTGCCGGTGCATGCTGCCCTGGCCGTCGGCGCGGTGCATCACCGCCTGGTGCAGACCGGGCTGCGCTGCGACTCCAACATCCTGGTGGAGACCGCCACCGCCCGCGATCCGCACCATTTCGCGGTGCTGATCGGCTTCGGCGCCTCGGCGGTCTATCCGTTCCTCGCCTACGAGGTGCTGGCCGACCTGATCCGCACCGGTGAAGTGCTGGGCGACCTCTACGAGGTGTTCAAGTACTACCGCAAGGGGATCTCCAAGGGCCTGATGAAGATTCTCTCGAAGATGGGCATCTCCACCGTCGCGTCCTACCGTGGCGCGCAGCTGTTCGAGGCGGTGGGCCTGTCCGACGAGGTCACCGACCTGTGCTTCACCGGAGTCGCCAGCCGCATCCAGGGCGCGCGCTTCGTCGATATCGAGAACGAGCAGAAGCTCCTGGCCGCCGAGGCCTGGAGCAACCGCAAGCCGATCCAGCAGGGCGGCTTGCTGAAGTTCGTCTATGGCGGCGAATACCACGCCTACAACCCGGATGTGGTGAATACCCTGCAGGCTGCCGTGCAGCAGGGCGACTACGAGAAGTTCAAGGAATACACCGCGCTGGTGGACCAGCGCCCAGTGTCGATGATCCGCGACCTGTTGCAGGTGAAGACCGCCGCGCAGCCGCTGGCGCTCGACGAGGTGGAGCCGCTGGAAGCCATCTTCAAGCGCTTCGATGCCGCCGGGATTTCCCTCGGCGCGCTGTCGCCGGAGGCCCACGAGGCGCTGGCCGAGGCGATGAACACCCTGGGCGGTCGCTCCAACTCCGGCGAGGGCGGCGAGGACCCGGCGCGCTACGGCACGTTGAAGAGTTCGAAGATCAAGCAGGTGGCTACCGGCCGCTTCGGCGTGACCCCGGAATACCTGGTCAACGCCGAGGTCCTGCAGATCAAGGTGGCCCAGGGTGCCAAGCCCGGCGAGGGCGGCCAGTTGCCGGGCGGCAAGGTCAACGGCCTGATCGCCCGCCTGCGCTATGCGGTGCCCGGCGTGACCCTGATCTCGCCGCCGCCGCACCACGACATCTACTCCATCGAAGACCTGGCGCAGCTGATCTTCGACCTCAAGCAGGTCAACCCGCAGGCGCTGGTATCGGTGAAGCTGGTATCCGAGCCGGGCGTCGGCACCATCGCCGCCGGCGTGGCCAAGGCCTATGCGGACCTGATCACCATCTCCGGCTACGACGGCGGTACCGGCGCCTCGCCGATCACCTCGATCAAGTACGCCGGCTCGCCCTGGGAACTGGGCCTGGCGGAAACCCACCAGACCCTGCGCGGCAACGACCTGCGCGGCAAGGTCCGGGTGCAGACCGATGGCGGCCTGAAGACAGGCCTGGACGTGATCAAGGCGGCCATTCTCGGCGCCGAGAGCTTCGGCTTCGGCACCGCGCCGATGATCGCCCTGGGCTGCAAGTACCTGCGCATCTGCCATCTGAACAACTGCGCCACCGGCGTGGCGACGCAGAACGACAAGTTGCGCAAGGACCACTTCATCGGCACCACCGCGATGGTGATCAACTTCTTCACCTTCATCGCCACGGAAACCCGCGAGTGGCTGGCGCGCCTCGGCGTGCGCAGCCTGGGCGAGCTGATCGGCCGCACCGACCTGCTGGAGATCCTCCCCGGCGAGACCGCCAAGCAGCAGAACCTGGATCTCGCTCCGTTGCTGGGCAGTGAGCTGATCCCGGCCGACAAGCCGCAGTTCTGCGAGGTGGAGAAAAACCCGCCGTTCGACCAGGGCCTGCTGGCCGAGAAGATGGTCGAGCTGTCGAAGGCCGCCATCGAAGGCCTGAGCGGCGGCGAGTACGAGCTGGACATCTGCAACTGCGACCGATCCATCGGCGCGCGGGTATCCGGCGAGATCGCCCGCCTGCACGGCAACCAGGGCATGGCCAAGGCACCGGTGGTGTTCCGTTTCAAGGGCACCGCGGGACAGAGCTTCGGCGTGTGGAACGCCGGTGGCCTGCACCTGTACCTGGAAGGCGACGCCAACGACTACGTAGGCAAGGGCATGACCGGCGGCAAGCTGGTGATCACCCCGCCCAAGGGCAGTCCGTTCAAGACCCAGGAATCGGCCATCGTCGGCAACACCTGCCTGTACGGCGCCACCGGCGGCAAGCTGTTCGCCGCCGGAACCGCCGGCGAGCGCTTCGCCGTGCGCAACTCCGGTTCCCATGCGGTGGTGGAAGGTACCGGCGACCACTGCTGCGAATACATGACCGGCGGTTTCGTCTGCGTGCTGGGCAAGACCGGCTACAACTTCGGCTCCGGCATGACCGGGGGCTTCGCCTACGTCCTCGACATGGACAACACTTTCGTCGACCGCGTGAACCACGAGCTGGTGGAAATCCAGCGGATCAGCGGCGAGGCGATGGAGGCCTATCGCAGCCACCTGCGCAAGGTCCTGGTCGAGTACGTGAACGAAACGGCAAGCGAGTGGGGCGCGAACATCCTGGAAAACCTGGATGACTACCTGCGTCGGTTCTGGCTGGTGAAACCGAAGGCGGCCAGCCTCGGTTCGCTGCTGACCAGCACCCGTGCCAACCCGCAATAA
Protein Properties
Protein Residues: 1481
Protein Molecular Weight: 161.6 kDa
Protein Theoretical pI: 6.03
Hydropathicity (GRAVY score): -0.143
Charge at pH 7 (predicted): -19.3
Protein Sequence: 
>PA5036
MKAGLYHPETFKDNCGFGLIAHMQGEPSHQLLQTAIEALTCMTHRGGINADGKTGDGCGLLIQKPDLFLRAVAREAFSVDLPEQYAVGMVFFNQDPVKAEAARENMNREIVAAGLELVGWRKVPIDTSVLGRLALERLPQIEQVFIGGAGLSDQDFAIKLFSARRRSSVANAADSDHYICSFSHKTIIYKGLMMPADLAAFYPDLGDERLQTAICVFHQRFSTNTLPKWPLAQPFRFLAHNGEINTITGNRNWAQARRTKFTNELIPDLEELGPLVNRVGSDSSSMDNMLELMVTGGMDLFRGLRMIIPPAWQNVETMDADLRAFYEYNSLHMEPWDGPAGVVLTDGRYAVCLLDRNGLRPSRWVTTKNGYITLASEIGVWDYKPEDVIAKGRVGPGQILAVDTETGQVLHSDDIDNRLKSRHPYKQWLRQNALRIQATLDDDHGVASYDADQLKQFMKMFQVTFEERDQVLRPLGEQGQEAVGSMGDDTPMAILSRRIRSPYDYFRQQFAQVTNPPIDPLREAIVMSLETCLGVEQNIFEEAPHHANQAILTTPVISPAKWRTIMTLDRPGFDRHFIDLNYDESMGLEAAVRNIADQAEEAVRGGKVLLILSDRHIAPGKLPVHAALAVGAVHHRLVQTGLRCDSNILVETATARDPHHFAVLIGFGASAVYPFLAYEVLADLIRTGEVLGDLYEVFKYYRKGISKGLMKILSKMGISTVASYRGAQLFEAVGLSDEVTDLCFTGVASRIQGARFVDIENEQKLLAAEAWSNRKPIQQGGLLKFVYGGEYHAYNPDVVNTLQAAVQQGDYEKFKEYTALVDQRPVSMIRDLLQVKTAAQPLALDEVEPLEAIFKRFDAAGISLGALSPEAHEALAEAMNTLGGRSNSGEGGEDPARYGTLKSSKIKQVATGRFGVTPEYLVNAEVLQIKVAQGAKPGEGGQLPGGKVNGLIARLRYAVPGVTLISPPPHHDIYSIEDLAQLIFDLKQVNPQALVSVKLVSEPGVGTIAAGVAKAYADLITISGYDGGTGASPITSIKYAGSPWELGLAETHQTLRGNDLRGKVRVQTDGGLKTGLDVIKAAILGAESFGFGTAPMIALGCKYLRICHLNNCATGVATQNDKLRKDHFIGTTAMVINFFTFIATETREWLARLGVRSLGELIGRTDLLEILPGETAKQQNLDLAPLLGSELIPADKPQFCEVEKNPPFDQGLLAEKMVELSKAAIEGLSGGEYELDICNCDRSIGARVSGEIARLHGNQGMAKAPVVFRFKGTAGQSFGVWNAGGLHLYLEGDANDYVGKGMTGGKLVITPPKGSPFKTQESAIVGNTCLYGATGGKLFAAGTAGERFAVRNSGSHAVVEGTGDHCCEYMTGGFVCVLGKTGYNFGSGMTGGFAYVLDMDNTFVDRVNHELVEIQRISGEAMEAYRSHLRKVLVEYVNETASEWGANILENLDDYLRRFWLVKPKAASLGSLLTSTRANPQ
References
External Links:
Resource Link
Genome ID: PA5036
Entrez Gene ID: 881222
NCBI Protein ID: 15600229
General Reference: PaperBLAST - Find papers about PA5036 and its homologs