UDP-N-acetylmuramoylalanine--D-glutamate ligase (PA4414)

Identification
Name: UDP-N-acetylmuramoylalanine--D-glutamate ligase
Synonyms:
  • D-glutamic acid-adding enzyme
  • UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase
Gene Name: murD
Enzyme Class:
Biological Properties
General Function: Involved in ATP binding
Specific Function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA)
Cellular Location: Cytoplasm
KEGG Pathways:
KEGG Reactions:
Thumb+Thumb+Thumb ↔ Thumb+Thumb+Thumb+Thumb
Adenosine triphosphate + D-Glutamic acid + UDP-N-Acetylmuramoyl-L-alanine ↔ ADP + Hydrogen ion + Phosphate + UDP-N-Acetylmuramoyl-L-alanyl-D-glutamate
ReactionCard
Thumb+Thumb+Thumb ↔ Thumb+Thumb+Thumb
Adenosine triphosphate + UDP-N-Acetylmuramoyl-L-alanine + D-Glutamic acid ↔ ADP + Phosphate + UDP-N-Acetylmuramoyl-L-alanyl-D-glutamate
ReactionCard
SMPDB Reactions:
Thumb+Thumb+Thumb → Adenosine diphosphate+Thumb+UDP-N-acetylmuramoyl-L-alanyl-D-glutamate+Thumb+Thumb
UDP-N-Acetylmuramoyl-L-alanine + Adenosine triphosphate + D-Glutamic acid → Adenosine diphosphate + Phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + ADP + UDP-N-Acetylmuramoyl-L-alanyl-D-glutamate
ReactionCard
Thumb+Thumb+Thumb → Thumb+Thumb+Thumb
UDP-N-Acetylmuramyl-L-Ala + Adenosine triphosphate + D-Glutamic acid → UDP-N-Acetylmuramoyl-L-alanyl-D-glutamate + ADP + Phosphate
ReactionCard
PseudoCyc/BioCyc Reactions:
Thumb+Thumb+Thumb ↔ Thumb+Thumb+Thumb+Thumb
Adenosine triphosphate + D-Glutamic acid + UDP-N-Acetylmuramoyl-L-alanine ↔ ADP + Hydrogen ion + Phosphate + UDP-N-Acetylmuramoyl-L-alanyl-D-glutamate
ReactionCard
Thumb+Thumb+Thumb → Thumb+Thumb+Thumb+Thumb
D-Glutamic acid + UDP-N-Acetylmuramoyl-L-alanine + Adenosine triphosphate → Hydrogen ion + UDP-N-Acetylmuramoyl-L-alanyl-D-glutamate + Phosphate + ADP
ReactionCard
Complex Reactions: Not Available
Transports: Not Available
Metabolites:
PAMDB IDNameView
PAMDB000148Adenosine triphosphateMetaboCard
PAMDB000345ADPMetaboCard
PAMDB000491D-Glutamic acidMetaboCard
PAMDB001633Hydrogen ionMetaboCard
PAMDB001776Inorganic phosphateMetaboCard
PAMDB000383PhosphateMetaboCard
PAMDB000648UDP-N-Acetylmuramoyl-L-alanineMetaboCard
PAMDB000649UDP-N-Acetylmuramoyl-L-alanyl-D-glutamateMetaboCard
PAMDB001300UDP-N-Acetylmuramyl-L-AlaMetaboCard
GO Classification:
Component
cell part
cytoplasm
intracellular part
Function
acid-amino acid ligase activity
adenyl nucleotide binding
adenyl ribonucleotide binding
ATP binding
binding
catalytic activity
ligase activity
ligase activity, forming carbon-nitrogen bonds
nucleoside binding
purine nucleoside binding
UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
Process
aminoglycan metabolic process
biological regulation
biosynthetic process
carbohydrate metabolic process
cell division
cell wall biogenesis
cell wall organization or biogenesis
cellular cell wall organization or biogenesis
cellular component organization or biogenesis
cellular process
glycosaminoglycan metabolic process
metabolic process
peptidoglycan biosynthetic process
peptidoglycan metabolic process
peptidoglycan-based cell wall biogenesis
polysaccharide metabolic process
primary metabolic process
regulation of biological quality
regulation of cell shape
Gene Properties
Locus tag: PA4414
Strand: -
Entrez Gene ID: 881268
Accession: NP_253104.1
GI: 15599610
Sequence start: 4947330
Sequence End: 4948676
Sequence Length: 1346
Gene Sequence: 
>PA4414
ATGAGCCTGATCGCCTCCGACCACTTCCGCATCGTTGTCGGCCTCGGCAAGAGCGGCATGTCCCTGGTGCGCTACCTGGCGCGCCGCGGCTTGCCTTTCGCCGTGGTCGATACCCGAGAGAACCCGCCGGAGCTGGCCACCCTGCGTGCCCAGTATCCGCAGGTGGAAGTGCGTTGCGGCGAACTCGACGCCGAGTTCCTCTGCTCCGCCCGCGAACTCTATGTCAGCCCCGGCTTGTCGCTGCGCACCCCTGCGCTGGTACAGGCCGCCGCGAAAGGCGTGCGCATCTCCGGTGACATCGATCTCTTCGCCCGCGAGGCGAAGGCCCCGATCGTCGCCATCACCGGTTCCAACGCGAAGAGCACCGTGACCACCCTGGTGGGCGAAATGGCGGTGGCCGCGGACAAGCGTGTCGCCGTCGGCGGCAACCTCGGCACCCCGGCGCTCGACCTGCTGGCCGACGACATCGAGCTGTACGTGTTGGAGCTGTCGAGCTTCCAGCTGGAAACCTGCGATCGCCTCAACGCCGAGGTGGCGACCGTGCTGAACGTCAGCGAAGACCATATGGATCGCTACGACGGCATGGCTGACTACCACCTGGCCAAGCACCGGATCTTCCGCGGTGCCCGCCAGGTCGTGGTGAATCGCGCCGATGCCCTGACCCGACCGCTGATCGCCGATACCGTGCCGTGCTGGTCGTTCGGCCTGAACAAGCCGGACTTCAAGGCTTTCGGCCTGATCGAGGAAGACGGCCAGAAGTGGCTGGCGTTCCAGTTCGACAAGCTGCTGCCGGTTGGCGAACTGAAGATCCGTGGCGCCCACAACTATTCCAACGCGCTCGCCGCGCTGGCGCTGGGCCATGCGGTCGGCCTGCCGTTCGACGCCATGCTCGGCGCGCTGAAGGCGTTTTCCGGCCTGGCTCATCGCTGCCAGTGGGTACGCGAGCGGCAGGGCGTGAGCTACTACGACGATTCCAAGGCCACCAACGTCGGCGCCGCCCTGGCGGCGATCGAGGGGCTGGGTGCCGACATCGACGGCAAGCTGGTGCTGCTCGCCGGCGGAGACGGCAAGGGCGCCGATTTCCATGACCTGCGCGAGCCGGTCGCGCGCTTCTGCCGGGCGGTGGTACTGCTTGGCCGTGACGCCGGGCTGATTGCCCAGGCACTGGGCAACGCGGTACCGCTGGTGCGCGTCGCAACGCTGGACGAAGCAGTCCGGCAGGCCGCCGAGCTGGCCCGCGAAGGCGATGCGGTGCTGTTGTCGCCGGCCTGCGCGAGCCTGGACATGTTCAAGAACTTCGAAGAACGCGGACGCCTGTTCGCCAAAGCCGTAGAGGAGCTAGCGTGA
Protein Properties
Protein Residues: 448
Protein Molecular Weight: 48.1 kDa
Protein Theoretical pI: 5.7
Hydropathicity (GRAVY score): 0.125
Charge at pH 7 (predicted): -6.27
Protein Sequence: 
>PA4414
MSLIASDHFRIVVGLGKSGMSLVRYLARRGLPFAVVDTRENPPELATLRAQYPQVEVRCGELDAEFLCSARELYVSPGLSLRTPALVQAAAKGVRISGDIDLFAREAKAPIVAITGSNAKSTVTTLVGEMAVAADKRVAVGGNLGTPALDLLADDIELYVLELSSFQLETCDRLNAEVATVLNVSEDHMDRYDGMADYHLAKHRIFRGARQVVVNRADALTRPLIADTVPCWSFGLNKPDFKAFGLIEEDGQKWLAFQFDKLLPVGELKIRGAHNYSNALAALALGHAVGLPFDAMLGALKAFSGLAHRCQWVRERQGVSYYDDSKATNVGAALAAIEGLGADIDGKLVLLAGGDGKGADFHDLREPVARFCRAVVLLGRDAGLIAQALGNAVPLVRVATLDEAVRQAAELAREGDAVLLSPACASLDMFKNFEERGRLFAKAVEELA
References
External Links:
Resource Link
Genome ID: PA4414
Entrez Gene ID: 881268
NCBI Protein ID: 15599610
General Reference: PaperBLAST - Find papers about PA4414 and its homologs