Undecaprenyl phosphate-alpha-4-amino-4-deoxy-L-arabinose arabinosyl transferase (PA3556)

Identification
Name: Undecaprenyl phosphate-alpha-4-amino-4-deoxy-L-arabinose arabinosyl transferase
Synonyms:
  • 4-amino-4-deoxy-L-arabinose lipid A transferase
  • Polymyxin resistance protein pmrK
  • Undecaprenyl phosphate-alpha-L-Ara4N transferase
Gene Name: arnT
Enzyme Class:
Biological Properties
General Function: Involved in transferase activity, transferring pentosyl groups
Specific Function: Catalyzes the transfer of the L-Ara4N moiety of the glycolipid undecaprenyl phosphate-alpha-L-Ara4N to lipid A. The modified arabinose is attached to lipid A and is required for resistance to polymyxin and cationic antimicrobial peptides
Cellular Location: Cell inner membrane; Multi-pass membrane protein
KEGG Pathways:
  • Cationic antimicrobial peptide (CAMP) resistance pae01503
KEGG Reactions:
Undecaprenyl phosphate alpha-L-Ara4N+Thumb ↔ Lipid IIA+Thumb
Undecaprenyl phosphate alpha-L-Ara4N + 2,3,2'3'-Tetrakis(3-hydroxytetradecanoyl)-D-glucosaminyl-1,6-beta-D-glucosamine 1,4'-bisphosphate ↔ Lipid IIA + Di-trans,poly-cis-undecaprenyl phosphate
ReactionCard
Undecaprenyl phosphate alpha-L-Ara4N+Di[3-deoxy-D-manno-octulosonyl]-lipid IV(A) ↔ alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[4-P-L-Ara4N]-lipid IVA+Thumb
Undecaprenyl phosphate alpha-L-Ara4N + Di[3-deoxy-D-manno-octulosonyl]-lipid IV(A) ↔ alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[4-P-L-Ara4N]-lipid IVA + Di-trans,poly-cis-undecaprenyl phosphate
ReactionCard
Thumb+Thumb+Thumb ↔ Thumb+Thumb+Thumb
undecaprenyl phosphate-4-amino-4-deoxy-L-arabinose + KDO2-Lipid A + 2,3,2'3'-Tetrakis(beta-hydroxymyristoyl)-D-glucosaminyl-1,6-beta-D-glucosamine 1,4'-bisphosphate ↔ alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[4-P-L-Ara4N]-lipid A + Di-trans,poly-cis-undecaprenyl phosphate + Lipid IIA
ReactionCard
SMPDB Reactions:
2 Thumb+Thumb → 2 di-trans,octa-cis-undecaprenyl diphosphate+Thumb
2 4-Amino-4-deoxy-alpha-L-arabinopyranosyl di-trans,octa-cis-undecaprenyl phosphate + (KDO)2-lipid A → 2 di-trans,octa-cis-undecaprenyl diphosphate + L-Ara4N-modified KDO2-Lipid A
ReactionCard
PseudoCyc/BioCyc Reactions:
Thumb+4-amino-4-deoxy-α-L-arabinopyranosyl ditrans,octacis-undecaprenyl phosphate → Thumb+Thumb
KDO2-Lipid A + 4-amino-4-deoxy-α-L-arabinopyranosyl ditrans,octacis-undecaprenyl phosphate → L-Ara4N-modified KDO2-Lipid A + Di-trans,poly-cis-undecaprenyl phosphate
ReactionCard
Complex Reactions:
core oligosaccharide lipid A+Thumb → Thumb+Thumb
core oligosaccharide lipid A + undecaprenyl phosphate-4-amino-4-deoxy-L-arabinose → 4-Amino-4-deoxy-L-arabinose modified core oligosaccharide lipid A + Undecaprenyl phosphate
ReactionCard
Transports: Not Available
Metabolites:
PAMDB IDNameView
PAMDB006296(KDO)2-lipid AMetaboCard
PAMDB0009062,3,2'3'-Tetrakis(3-hydroxytetradecanoyl)-D-glucosaminyl-1,6-beta-D-glucosamine 1,4'-bisphosphateMetaboCard
PAMDB0015452,3,2'3'-Tetrakis(beta-hydroxymyristoyl)-D-glucosaminyl-1,6-beta-D-glucosamine 1,4'-bisphosphateMetaboCard
PAMDB0034904-Amino-4-deoxy-alpha-L-arabinopyranosyl di-trans,octa-cis-undecaprenyl phosphateMetaboCard
PAMDB0018924-Amino-4-deoxy-L-arabinoseMetaboCard
PAMDB0015824-Amino-4-deoxy-L-arabinose modified core oligosaccharide lipid AMetaboCard
PAMDB001145Di-trans,poly-cis-undecaprenyl phosphateMetaboCard
PAMDB001643KDO(2)-lipid IV(A)MetaboCard
PAMDB001447KDO2-Lipid AMetaboCard
PAMDB001379L-Ara4N-modified KDO2-Lipid AMetaboCard
PAMDB003413lipid II(A)MetaboCard
PAMDB005112Lipid IIAMetaboCard
PAMDB001054Undecaprenyl phosphateMetaboCard
PAMDB001760undecaprenyl phosphate-4-amino-4-deoxy-L-arabinoseMetaboCard
GO Classification:
Component
cell part
membrane
Function
catalytic activity
mannosyltransferase activity
transferase activity
transferase activity, transferring glycosyl groups
transferase activity, transferring hexosyl groups
Process
macromolecule metabolic process
macromolecule modification
metabolic process
protein amino acid glycosylation
protein amino acid O-linked glycosylation
protein modification process
Gene Properties
Locus tag: PA3556
Strand: +
Entrez Gene ID: 879122
Accession: NP_252246.1
GI: 15598752
Sequence start: 3984890
Sequence End: 3986539
Sequence Length: 1649
Gene Sequence: 
>PA3556
ATGAGCCGCCGCCAGACCTGTTCGCTGCTGCTGATCGCCTTCGGCCTGTTCTACCTGGTTCCATTGAGCAACCATGGCCTGTGGATTCCCGACGAGACCCGCTACGCGCAGATCAGCCAGGCGATGCTGCTGGGCGGCGACTGGGTGTCGCCGCACTTCCTCGGCCTGCGCTACTTCGAGAAGCCGGTCGCCGGCTACTGGATGATCGCCCTCGGCCAGGCGGTCTTCGGCGAAAACCTGTTCGGCGTGCGCATCGCCTCGGTCGTCGCCACCGCCCTCAGCGTCCTGCTCGCCTACCTGCTGGCCCGCCGCCTGTGGCGCGACCCGCGCACCAGCCTGGCATGCGCGCTGCTCTACGCCAGCTTCGGCTTGATCGCCGGGCAGTCCGGCTATGCCAACCTCGACCCGCAATTCACCTTCTGGGTCAACCTGAGCCTGGTGGCGCTGTGGCATGCCCTCGACGCGGGGAGCCGCCGCGCGCGCCTGCTCGGCTGGACCCTGCTCGGCCTCGCCTGCGGCATGGGCTTCCTGACCAAGGGCTTCCTCGCCTGGCTGCTGCCGGTACTGGTCGCCCTGCCCTACATGCTCTGGCAGCGGCGCTGGCGCGAGCTGCTTGGCTACGGCGCGCTGGCGGTGCTGGCGGCGCTGCTCGTCTGCCTGCCCTGGGCGCTCGCCGTGCATGCACGGGAAGCGGACTACTGGCGGTTCTTCTTCTGGCACGAACACATCCGCCGCTTCGCCGGCGAAGACGCCCAGCACTCTCGCCCGTGGTGGTTCTACCTGCCGTTGCTGGCGGTCGCCTGCCTGCCCTGGAGCGGCCTGCTGCCGAGCGCTCTGCGCCAGGCCTGGCACGAGCGGCGCCAGGCGCCGGTGGTCTTCCTGGCGCTATGGCTGCTGTTGCCGCTGGCGTTCTTCAGCCTGAGCAGGGGCAAGCTGCCGACCTACATCATGCCCTGCCTGCTGCCGCTGGCACTGCTCATGGGCCACGCCCTGGTGCAGCGGCTGCGCCTGGGGAACAGCGTCGCGCTGCGCGGCAACGGGCTGCTCAACCTGGGCCTGGCGCTCCTCGCGCTGGCGGCCCTGGCCTACCTGCAACTGCGCAAGCCGGTGTACCAGGAAGAACCCTTCGAGCTGTTCCTGGTCCTGCTGGTGATCGGCGCCTGGGCCGCCGCCGGCCTCGCCCAGTGGCGCTACCCGCTACGCGCCTGGGCCGCGCCGCTGCTGGCGAGCTGGGTGCTGATCGCGCTGCTGCCGGCAGCGATGCCCAACCATGTGGTGCAGAACAAGACCCCCGACCTGTTCGTCGCCGAACACCTCGATGAACTGACCGGCGCCCGCCATCTGCTGAGCAATGACCTCGGCGCCGCCTCGGCCCTCGCCTGGCGCCTGCGTCGTAGCGACGTGACGCTCTACGACACGCGCGGCGAGCTGAAGTACGGCCTCTCCTACCCCGAGCACAGCCAGCGCAGCGTGCCCTTGGCAGACATCCGCCAGTGGCTCTGGCGGGCACGCCAGGACGGCTCGATTGCCGTGCTGCTGCGGATCAACAGCGCCAGCGACCGCTACCAGCTGGCGCTGTTGCCGGGCGACGGCGAACGCTACCGGAACGGCAACCTGGTCCTGGCGATCCTGCCGCAGGTGCGCCCATGA
Protein Properties
Protein Residues: 549
Protein Molecular Weight: 61.7 kDa
Protein Theoretical pI: 10.1
Hydropathicity (GRAVY score): 0.399
Charge at pH 7 (predicted): 20.93
Protein Sequence: 
>PA3556
MSRRQTCSLLLIAFGLFYLVPLSNHGLWIPDETRYAQISQAMLLGGDWVSPHFLGLRYFEKPVAGYWMIALGQAVFGENLFGVRIASVVATALSVLLAYLLARRLWRDPRTSLACALLYASFGLIAGQSGYANLDPQFTFWVNLSLVALWHALDAGSRRARLLGWTLLGLACGMGFLTKGFLAWLLPVLVALPYMLWQRRWRELLGYGALAVLAALLVCLPWALAVHAREADYWRFFFWHEHIRRFAGEDAQHSRPWWFYLPLLAVACLPWSGLLPSALRQAWHERRQAPVVFLALWLLLPLAFFSLSRGKLPTYIMPCLLPLALLMGHALVQRLRLGNSVALRGNGLLNLGLALLALAALAYLQLRKPVYQEEPFELFLVLLVIGAWAAAGLAQWRYPLRAWAAPLLASWVLIALLPAAMPNHVVQNKTPDLFVAEHLDELTGARHLLSNDLGAASALAWRLRRSDVTLYDTRGELKYGLSYPEHSQRSVPLADIRQWLWRARQDGSIAVLLRINSASDRYQLALLPGDGERYRNGNLVLAILPQVRP
References
External Links:
Resource Link
Genome ID: PA3556
Entrez Gene ID: 879122
NCBI Protein ID: 15598752
General Reference: PaperBLAST - Find papers about PA3556 and its homologs