2-oxoglutarate dehydrogenase E1 component (PA1585)

Identification
Name: 2-oxoglutarate dehydrogenase E1 component
Synonyms:
  • Alpha-ketoglutarate dehydrogenase
Gene Name: sucA
Enzyme Class:
Biological Properties
General Function: Involved in oxoglutarate dehydrogenase (succinyl-transferring) activity
Specific Function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components:2- oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and lipoamide dehydrogenase (E3)
Cellular Location: Not Available
KEGG Pathways:
KEGG Reactions:
Thumb+Thumb+Thumb ↔ Thumb+Thumb+Thumb+Thumb
Oxoadipic acid + Coenzyme A + NAD ↔ Glutaryl-CoA + Carbon dioxide + NADH + Hydrogen ion
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Thumb+Enzyme N6-(lipoyl)lysine ↔ [Dihydrolipoyllysine-residue succinyltransferase] S-glutaryldihydrolipoyllysine+Thumb
Oxoadipic acid + Enzyme N6-(lipoyl)lysine ↔ [Dihydrolipoyllysine-residue succinyltransferase] S-glutaryldihydrolipoyllysine + Carbon dioxide
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Thumb ↔ [Dihydrolipoyllysine-residue succinyltransferase] S-succinyldihydrolipoyllysine+Thumb
alpha-Ketoglutarate ↔ [Dihydrolipoyllysine-residue succinyltransferase] S-succinyldihydrolipoyllysine + Carbon dioxide
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SMPDB Reactions:
Thumb+Thumb+Thumb → Thumb+Thumb+Succinyl-CoA+Thumb
Oxoglutaric acid + NAD + Coenzyme A → NADH + Carbon dioxide + Succinyl-CoA + Succinyl-CoA
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a [2-oxoglutarate dehydrogenase E2 protein] N6-lipoyl-L-lysine+Thumb+Thumb → a [2-oxoglutarate dehydrogenase E2 protein] N6-S-succinyldihydrolipoyl-L-lysine+Thumb
a [2-oxoglutarate dehydrogenase E2 protein] N6-lipoyl-L-lysine + Oxoglutaric acid + Hydrogen ion → a [2-oxoglutarate dehydrogenase E2 protein] N6-S-succinyldihydrolipoyl-L-lysine + Carbon dioxide
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PseudoCyc/BioCyc Reactions:
Thumb+Thumb+Thumb ↔ Thumb+Thumb+Thumb+Thumb
Oxoadipic acid + Coenzyme A + NAD ↔ Glutaryl-CoA + Carbon dioxide + NADH + Hydrogen ion
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Thumb+Enzyme N6-(lipoyl)lysine ↔ [Dihydrolipoyllysine-residue succinyltransferase] S-glutaryldihydrolipoyllysine+Thumb
Oxoadipic acid + Enzyme N6-(lipoyl)lysine ↔ [Dihydrolipoyllysine-residue succinyltransferase] S-glutaryldihydrolipoyllysine + Carbon dioxide
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Thumb+[dihydrolipoyllysine-residue succinyltransferase] lipoyllysine → [dihydrolipoyllysine-residue succinyltransferase] S-succinyldihydrolipoyllysine+Thumb
Oxoglutaric acid + [dihydrolipoyllysine-residue succinyltransferase] lipoyllysine → [dihydrolipoyllysine-residue succinyltransferase] S-succinyldihydrolipoyllysine + Carbon dioxide
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Thumb ↔ [Dihydrolipoyllysine-residue succinyltransferase] S-succinyldihydrolipoyllysine+Thumb
alpha-Ketoglutarate ↔ [Dihydrolipoyllysine-residue succinyltransferase] S-succinyldihydrolipoyllysine + Carbon dioxide
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Thumb+Thumb+Thumb → Thumb+Thumb+Succinyl-CoA+Thumb
Oxoglutaric acid + NAD + Coenzyme A → NADH + Carbon dioxide + Succinyl-CoA + Succinyl-CoA
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Thumb
glycine + a tetrahydrofolate + NAD+ → a 5,10-methylene-tetrahydrofolate + ammonium + CO2 + NADH
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Thumb
pyruvate + coenzyme A + NAD+ → acetyl-CoA + CO2 + NADH
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Thumb
pyruvate + lipoamide → S-acetyldihydrolipoamide + CO2
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Thumb
acetyl-CoA + dihydrolipoamide ← S-acetyldihydrolipoamide + coenzyme A
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Thumb
2-oxoglutarate + coenzyme A + NAD+ → succinyl-CoA + CO2 + NADH
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Thumb
a [glycine-cleavage complex H protein] N6-aminomethyldihydrolipoyl-L-lysine + a tetrahydrofolate ↔ a [glycine-cleavage complex H protein] N6-dihydrolipoyl-L-lysine + a 5,10-methylene-tetrahydrofolate + ammonium
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Thumb
glycine + a [glycine-cleavage complex H protein] N6-lipoyl-L-lysine + H+ ↔ a [glycine-cleavage complex H protein] N6-aminomethyldihydrolipoyl-L-lysine + CO2
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Thumb
succinyl-lipoate + coenzyme A → succinyl-CoA + DIHYDROLIPOYL-OXO-GLUT-E2
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Thumb
a [lipoyl-carrier protein] N6-dihydrolipoyl-L-lysine + NAD+ = a [lipoyl-carrier protein]-N6-lipoyl-L-lysine + NADH + H+
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Thumb
2-oxoglutarate + a [2-oxoglutarate dehydrogenase E2 protein] N6-lipoyl-L-lysine + H+ → a [2-oxoglutarate dehydrogenase E2 protein] N6-S-succinyldihydrolipoyl-L-lysine + CO2
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Thumb
succinyl-CoA + a [2-oxoglutarate dehydrogenase E2 protein] N6-dihydrolipoyl-L-lysine = a [2-oxoglutarate dehydrogenase E2 protein] N6-S-succinyldihydrolipoyl-L-lysine + coenzyme A
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Thumb
a [2-oxoglutarate dehydrogenase E2 protein] N6-dihydrolipoyl-L-lysine + NAD+ = a [2-oxoglutarate dehydrogenase E2 protein] N6-lipoyl-L-lysine + NADH + H+
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Complex Reactions:
Thumb+Thumb+Thumb → Thumb+Thumb+Thumb
alpha-Ketoglutarate + Coenzyme A + NAD → Carbon dioxide + NADH + Succinyl-CoA
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Thumb+[dihydrolipoyllysine-residue succinyltransferase] lipoyllysine → [dihydrolipoyllysine-residue succinyltransferase] S-succinyldihydrolipoyllysine+Thumb
Oxoglutaric acid + [dihydrolipoyllysine-residue succinyltransferase] lipoyllysine → [dihydrolipoyllysine-residue succinyltransferase] S-succinyldihydrolipoyllysine + Carbon dioxide
ReactionCard
Transports: Not Available
Metabolites:
PAMDB IDNameView
PAMDB000466alpha-KetoglutarateMetaboCard
PAMDB000568Carbon dioxideMetaboCard
PAMDB000381Coenzyme AMetaboCard
PAMDB000344Glutaryl-CoAMetaboCard
PAMDB001633Hydrogen ionMetaboCard
PAMDB000197NADMetaboCard
PAMDB000398NADHMetaboCard
PAMDB000093Oxoadipic acidMetaboCard
PAMDB000623Oxoglutaric acidMetaboCard
PAMDB000228Succinyl-CoAMetaboCard
PAMDB000361Thiamine pyrophosphateMetaboCard
GO Classification:
Function
binding
catalytic activity
oxidoreductase activity
oxidoreductase activity, acting on the aldehyde or oxo group of donors
oxidoreductase activity, acting on the aldehyde or oxo group of donors, disulfide as acceptor
oxoglutarate dehydrogenase (succinyl-transferring) activity
thiamin pyrophosphate binding
vitamin binding
Process
alcohol metabolic process
glucose catabolic process
glucose metabolic process
glycolysis
hexose metabolic process
metabolic process
monosaccharide metabolic process
small molecule metabolic process
Gene Properties
Locus tag: PA1585
Strand: +
Entrez Gene ID: 881879
Accession: NP_250276.1
GI: 15596782
Sequence start: 1724244
Sequence End: 1727075
Sequence Length: 2831
Gene Sequence: 
>PA1585
ATGCACGAAAGCGTAATGCAGCGGATGTGGAACAGTGCCCATCTATCCGGTGGTAACGCTGCCTACGTCGAAGAGCTCTACGAGCTCTACCTGCACGACCCTAACGCTGTGCCAGAAGAGTGGCGCACGTATTTCCAGAAGCTTCCCGCCGATGGCAATCCCGCCCCCGACGTCTCGCACTCCACAGTGCGCGATCATTTCGTCCTGCTGGCCAAGAACCAGCGCCGTGCCCAGCCGGTTTCCGCCGGTAGCGTGAGCAGCGAGCACGAGAAGAAGCAGGTCGAAGTCCTTCGTCTCATTCACGCTTACCGTCTCCGCGGCCACCAGGCTTCCACCCTCGACCCGCTCGGCCTGTGGCAGCGTCCCGCGCCTGCCGACCTGTCGATCGACCACTACGGTCTGACGGGTGCCGACCTGGACACCACTTTCCGCACCGGTGAGCTTTACATCGGCAAGGAAGAGGCGACCCTGCGTGAAATCGTCGACAGCCTGAAGAGCACCTACTGCGGTACCTTCGGCGCCGAGTTCATGCACATCGTCGATTCCGAGCAGCGCAAGTGGTTCCTCCAGCGTCTGGAAAGCGTGCGTGGTCGTCCCGGCTTCTCCGCCGAGGCGCGTGCGCACCTGCTCGAGCGCCTGACCGCGGCCGAAGGCCTGGAGAAGTACCTGGGCACCAAGTACCCGGGCACCAAGCGTTTCGGCCTGGAAGGCGGCGAAAGCCTGATCCCGATGGTCGACGAGATCATCCAGCGCTGCGGTTCCTACGGCGCCAAGGAAATCGTCATCGGCATGGCCCACCGCGGTCGCCTGAACGTCCTGGTCAACACCCTGGGCAAGAACCCGCGCGACCTGTTCGACGAGTTCGAAGGCAAGAAGATCGTCGAGCTGGGCTCCGGTGACGTGAAGTACCACCAGGGCTTCTCCTCGAACGTGATGACCAGCGGTGGCGAAGTCCACCTGGCGCTGGCGTTCAACCCGTCCCACCTGGAAATCGTTTCCCCGGTGGTCGAGGGTTCCGTTCGTGCTCGCCAGGACCGCCGCAAGGACAGCTCCGGCGACAAGGTGGTACCGATTTCCATCCACGGCGATGCCGCCTTCGCGGGGCAGGGCGTGGTCATGGAAACCTTCCAGATGTCGCAGACCCGCGCCTACAAGACCGGTGGCACCATCCACCTGGTGATCAACAACCAGGTCGGTTTCACCACCAGCCGCCAGGACGATGCGCGTTCCACCGAGTACGCCACCGACGTGGCGAAGATGATTCAGGCGCCGATCTTCCACGTCAACGGCGACGATCCCGAAGCCGTGCTGTTCGTCACCCAACTGGCCGTCGACTACCGCATGCAGTTCAAGCGTGACGTGGTCATCGACCTGGTCTGCTACCGTCGCCGCGGCCACAACGAGGCGGACGAGCCGAGCGGCACCCAGCCGCTGATGTACCAGCAGATCGCCAAGCAGCGTACTACCCGCGAGCTGTACGCCGATGCCCTGGTGAATGCCGGCGTGCTCAGCGCCGAGCAGGTTCAGTCGAAGATCGACGACTACCGCGACGCCCTTGACAACGGCCTGCACGTGGTGAAGAGCCTGGTCAAGGAACCCAACAAGGAACTGTTCGTCGACTGGCGTCCGTACCTGGGTCATGCCTGGACCGCGCGTCACGACACCCGCTTCGACCTGAAGACCCTGCAGGAACTGTCCAGCAAGATGCTGGAAGTGCCGGAAGGCTTCGTGGTTCAGCGCCAGGTTTCGAAGATCTACGAAGATCGCCAGAAGATGGCGGCCGGTGGCCTGCCGATCAACTGGGGCTTCGCCGAGACCCTGGCCTACGCGACCCTGCTGTTCGAAGGCCATCCGGTACGGATGACCGGCCAGGACGTCGGCCGTGGCACCTTCTCGCACCGTCACGCGGTGCTGCACAACCAGAAGGATGACTCGGTCTACGTACCGCTGGCCAATCTGTTCGACGGCCAGCCGCGCCTGGATATCTACGATTCCTTCCTTTCGGAAGAGGCCGTGCTGGCGTTCGAATACGGTTTCGCCACCACCACGCCGAACTCGCTGGTGATCTGGGAAGCCCAGTTCGGCGACTTCGCCAACGGCGCCCAGGTGGTCATCGACCAGTTCATCACCAGTGGCGAAAGCAAATGGGGCCGACTCTGCGGCCTGACCATGCTGCTGCCGCACGGCTATGAGGGGCAGGGCCCGGAGCACTCCTCCGCGCGCCTGGAGCGCTACCTGCAGCTCTGTGCCGAGCAGAACATCCAGGTCTGCGTGCCGACCACCCCGGCTCAGGTCTACCACATGCTCCGCCGTCAGGTGATCCGCCCGCTGCGCAAGCCGCTGGTGGTGATGACGCCGAAGTCCCTGCTGCGTCACAAGCTCGCCATCTCGACCCTGGAAGATCTGGCCAACGGTTCCTTCCAGACTGTGATCCCGGAAATCGACAGCCTGGATCCGAAGAAGGTCGACCGCGTCGTGCTGTGCAGCGGCAAGGTGTACTACGACCTGCTGGAGAAGCGTCGCGCGGAAGGCCGCGAAGATACCGCGATCGTCCGTATCGAGCAGCTGTATCCGTTCCCGGAAGACGACCTGGCGGAAGTTCTGGCTCCGTACAAGAACCTCAAGCACATCGTCTGGTGCCAGGAAGAGCCGATGAACCAGGGCGCCTGGTTCTGCAGCCAGCATCACATGCGTCGTGTCATCGCCGCGCACAAGAAGGGCCTCAATCTCGAGTACGCCGGCCGCGAAGGCTCCGCTGCTCCGGCTTGCGGCTACGCCTCGATGCACGCCGAGCAGCAGGAAAAACTGCTGCAAGACGCCTTTACTGTTTAA
Protein Properties
Protein Residues: 943
Protein Molecular Weight: 105.9 kDa
Protein Theoretical pI: 6.54
Hydropathicity (GRAVY score): -0.378
Charge at pH 7 (predicted): -8.83
Protein Sequence: 
>PA1585
MHESVMQRMWNSAHLSGGNAAYVEELYELYLHDPNAVPEEWRTYFQKLPADGNPAPDVSHSTVRDHFVLLAKNQRRAQPVSAGSVSSEHEKKQVEVLRLIHAYRLRGHQASTLDPLGLWQRPAPADLSIDHYGLTGADLDTTFRTGELYIGKEEATLREIVDSLKSTYCGTFGAEFMHIVDSEQRKWFLQRLESVRGRPGFSAEARAHLLERLTAAEGLEKYLGTKYPGTKRFGLEGGESLIPMVDEIIQRCGSYGAKEIVIGMAHRGRLNVLVNTLGKNPRDLFDEFEGKKIVELGSGDVKYHQGFSSNVMTSGGEVHLALAFNPSHLEIVSPVVEGSVRARQDRRKDSSGDKVVPISIHGDAAFAGQGVVMETFQMSQTRAYKTGGTIHLVINNQVGFTTSRQDDARSTEYATDVAKMIQAPIFHVNGDDPEAVLFVTQLAVDYRMQFKRDVVIDLVCYRRRGHNEADEPSGTQPLMYQQIAKQRTTRELYADALVNAGVLSAEQVQSKIDDYRDALDNGLHVVKSLVKEPNKELFVDWRPYLGHAWTARHDTRFDLKTLQELSSKMLEVPEGFVVQRQVSKIYEDRQKMAAGGLPINWGFAETLAYATLLFEGHPVRMTGQDVGRGTFSHRHAVLHNQKDDSVYVPLANLFDGQPRLDIYDSFLSEEAVLAFEYGFATTTPNSLVIWEAQFGDFANGAQVVIDQFITSGESKWGRLCGLTMLLPHGYEGQGPEHSSARLERYLQLCAEQNIQVCVPTTPAQVYHMLRRQVIRPLRKPLVVMTPKSLLRHKLAISTLEDLANGSFQTVIPEIDSLDPKKVDRVVLCSGKVYYDLLEKRRAEGREDTAIVRIEQLYPFPEDDLAEVLAPYKNLKHIVWCQEEPMNQGAWFCSQHHMRRVIAAHKKGLNLEYAGREGSAAPACGYASMHAEQQEKLLQDAFTV
References
External Links:
Resource Link
Genome ID: PA1585
Entrez Gene ID: 881879
NCBI Protein ID: 15596782
General Reference: PaperBLAST - Find papers about PA1585 and its homologs