Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex (PA1586)

Identification
Name: Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex
Synonyms:
  • 2-oxoglutarate dehydrogenase complex component E2
  • OGDC-E2
  • Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex
Gene Name: sucB
Enzyme Class:
Biological Properties
General Function: Involved in transferase activity, transferring acyl groups
Specific Function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components:2- oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and lipoamide dehydrogenase (E3)
Cellular Location: Not Available
KEGG Pathways:
KEGG Reactions:
Thumb+Enzyme N6-(dihydrolipoyl)lysine+Thumb ↔ Thumb+[Dihydrolipoyllysine-residue succinyltransferase] S-succinyldihydrolipoyllysine
Succinyl-CoA + Enzyme N6-(dihydrolipoyl)lysine + Enzyme N6-(dihydrolipoyl)lysine ↔ Coenzyme A + [Dihydrolipoyllysine-residue succinyltransferase] S-succinyldihydrolipoyllysine
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Thumb+Enzyme N6-(dihydrolipoyl)lysine ↔ Thumb+[Dihydrolipoyllysine-residue succinyltransferase] S-glutaryldihydrolipoyllysine
Glutaryl-CoA + Enzyme N6-(dihydrolipoyl)lysine ↔ Coenzyme A + [Dihydrolipoyllysine-residue succinyltransferase] S-glutaryldihydrolipoyllysine
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SMPDB Reactions:
Thumb+Thumb+Thumb → Thumb+Thumb+Succinyl-CoA+Thumb
Oxoglutaric acid + NAD + Coenzyme A → NADH + Carbon dioxide + Succinyl-CoA + Succinyl-CoA
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a [2-oxoglutarate dehydrogenase E2 protein] N6-S-succinyldihydrolipoyl-L-lysine+Thumb → a [2-oxoglutarate dehydrogenase E2 protein] N6-dihydrolipoyl-L-lysine+Thumb
a [2-oxoglutarate dehydrogenase E2 protein] N6-S-succinyldihydrolipoyl-L-lysine + Succinyl-CoA → a [2-oxoglutarate dehydrogenase E2 protein] N6-dihydrolipoyl-L-lysine + Coenzyme A
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PseudoCyc/BioCyc Reactions:
Thumb+Enzyme N6-(dihydrolipoyl)lysine+Thumb ↔ Thumb+[Dihydrolipoyllysine-residue succinyltransferase] S-succinyldihydrolipoyllysine
Succinyl-CoA + Enzyme N6-(dihydrolipoyl)lysine + Enzyme N6-(dihydrolipoyl)lysine ↔ Coenzyme A + [Dihydrolipoyllysine-residue succinyltransferase] S-succinyldihydrolipoyllysine
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Thumb+Enzyme N6-(dihydrolipoyl)lysine ↔ Thumb+[Dihydrolipoyllysine-residue succinyltransferase] S-glutaryldihydrolipoyllysine
Glutaryl-CoA + Enzyme N6-(dihydrolipoyl)lysine ↔ Coenzyme A + [Dihydrolipoyllysine-residue succinyltransferase] S-glutaryldihydrolipoyllysine
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Thumb+Thumb+Thumb → Thumb+Thumb+Succinyl-CoA+Thumb
Oxoglutaric acid + NAD + Coenzyme A → NADH + Carbon dioxide + Succinyl-CoA + Succinyl-CoA
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Thumb
glycine + a tetrahydrofolate + NAD+ → a 5,10-methylene-tetrahydrofolate + ammonium + CO2 + NADH
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Thumb
pyruvate + coenzyme A + NAD+ → acetyl-CoA + CO2 + NADH
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Thumb
pyruvate + lipoamide → S-acetyldihydrolipoamide + CO2
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Thumb
acetyl-CoA + dihydrolipoamide ← S-acetyldihydrolipoamide + coenzyme A
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Thumb
2-oxoglutarate + coenzyme A + NAD+ → succinyl-CoA + CO2 + NADH
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Thumb
a [glycine-cleavage complex H protein] N6-aminomethyldihydrolipoyl-L-lysine + a tetrahydrofolate ↔ a [glycine-cleavage complex H protein] N6-dihydrolipoyl-L-lysine + a 5,10-methylene-tetrahydrofolate + ammonium
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Thumb
glycine + a [glycine-cleavage complex H protein] N6-lipoyl-L-lysine + H+ ↔ a [glycine-cleavage complex H protein] N6-aminomethyldihydrolipoyl-L-lysine + CO2
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Thumb
succinyl-lipoate + coenzyme A → succinyl-CoA + DIHYDROLIPOYL-OXO-GLUT-E2
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Thumb
a [lipoyl-carrier protein] N6-dihydrolipoyl-L-lysine + NAD+ = a [lipoyl-carrier protein]-N6-lipoyl-L-lysine + NADH + H+
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Thumb
2-oxoglutarate + a [2-oxoglutarate dehydrogenase E2 protein] N6-lipoyl-L-lysine + H+ → a [2-oxoglutarate dehydrogenase E2 protein] N6-S-succinyldihydrolipoyl-L-lysine + CO2
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Complex Reactions:
Thumb+Thumb+Thumb → Thumb+Thumb+Thumb
alpha-Ketoglutarate + Coenzyme A + NAD → Carbon dioxide + NADH + Succinyl-CoA
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Transports: Not Available
Metabolites:
PAMDB IDNameView
PAMDB000466alpha-KetoglutarateMetaboCard
PAMDB000568Carbon dioxideMetaboCard
PAMDB000381Coenzyme AMetaboCard
PAMDB004301Enzyme N6-(dihydrolipoyl)lysineMetaboCard
PAMDB000344Glutaryl-CoAMetaboCard
PAMDB001633Hydrogen ionMetaboCard
PAMDB000197NADMetaboCard
PAMDB000398NADHMetaboCard
PAMDB000623Oxoglutaric acidMetaboCard
PAMDB000228Succinyl-CoAMetaboCard
GO Classification:
Component
macromolecular complex
oxoglutarate dehydrogenase complex
protein complex
Function
acyltransferase activity
binding
catalytic activity
dihydrolipoyllysine-residue succinyltransferase activity
protein binding
S-acyltransferase activity
S-succinyltransferase activity
transferase activity
transferase activity, transferring acyl groups
transferase activity, transferring acyl groups other than amino-acyl groups
Process
acetyl-CoA catabolic process
acetyl-CoA metabolic process
cellular metabolic process
coenzyme metabolic process
cofactor metabolic process
metabolic process
tricarboxylic acid cycle
Gene Properties
Locus tag: PA1586
Strand: +
Entrez Gene ID: 881930
Accession: NP_250277.1
GI: 15596783
Sequence start: 1727118
Sequence End: 1728347
Sequence Length: 1229
Gene Sequence: 
>PA1586
ATGGCTATCGAAATCAAAGCCCCAACCTTCCCGGAATCGGTCGCCGACGGCACCGTCGCCACCTGGCACAAGAAGCCGGGCGAAGCAGTCAAGCGCGACGAACTGATCGTCGACATCGAGACCGACAAGGTGGTCATCGAGGTTCTCGCCGAGGCTGACGGCGTGCTGGCCGAGATCATCAAGAACGAAGGCGACACCGTTCTGAGCAACGAGCTGCTCGGCAAGCTGAACGAAGGCGGCGCCGCCGCTCCGGCCGCTCCGGCTGCCGCTGCGCCCGCCGCCGCTCCGGCTGCACAGGCCGCCGCTCCGGCCGCCGCTGGCGGCGACGACGCCATCCTGTCCCCGGCCGCGCGCAAGCTGGCCGAGGAGGCCGGCATCGATCCGAACAGCATCGCCGGCACCGGCAAGGGCGGTCGCGTGACCAAGGAAGACGTGGTCGCTGCGGTCGAAGCGAAGAAGAACGCCCCGGCCGCCCCGGCCAAGCCTGCCGCCCCGGCTGCCGAGGCGCCGATCTTCGCCGCCGGCGACCGTGTCGAGAAGCGCGTGCCGATGACCCGCCTGCGGGCCAAGGTCGCCGAGCGCCTGGTCGAGGCGCAGTCCGCCATGGCCATGCTGACCACCTTCAACGAGGTCAACATGAAGCCGATCATGGACCTGCGTTCGAAGTACAAGGACCTGTTCGAGAAGAAGCACAACGGCGTGCGCCTGGGCTTCATGTCCTTCTTCGTCAAGGCCGCCACCGAGGCGCTGAAGCGCTTCCCGGGGGTCAACGCTTCCATCGACGGCAACGACATCGTCTACCACGGCTACCAGGACATCGGCGTTGCCGTTTCCAGCGACCGCGGCCTGGTGGTTCCGGTACTGCGTAACGCCGAGTTCATGAGCCTGGCCGAGATCGAGGGCGGCATCGCCAACTTCGGCAAGAAGGCGAAGGAAGGCAAGCTGACCATCGAAGACATGACCGGCGGCACCTTCACCATCTCCAACGGTGGCGTGTTCGGTTCCCTGCTGTCGACCCCGATCGTCAACCCGCCGCAGACCGCCATCCTCGGCATGCACAAGATCCAGGAGCGCCCGATGGCCGTGAACGGCCAGGTGGTGATCCTGCCGATGATGTATCTGGCGCTGTCCTACGACCACCGTCTGATCGACGGCAAGGAAGCGGTGAGCTTCCTGGTGGCGATCAAGGACCTGCTGGAAGATCCGGCTCGCCTGCTGCTGGACGTCTGA
Protein Properties
Protein Residues: 409
Protein Molecular Weight: 42.9 kDa
Protein Theoretical pI: 5.21
Hydropathicity (GRAVY score): 0.055
Charge at pH 7 (predicted): -6.78
Protein Sequence: 
>PA1586
MAIEIKAPTFPESVADGTVATWHKKPGEAVKRDELIVDIETDKVVIEVLAEADGVLAEIIKNEGDTVLSNELLGKLNEGGAAAPAAPAAAAPAAAPAAQAAAPAAAGGDDAILSPAARKLAEEAGIDPNSIAGTGKGGRVTKEDVVAAVEAKKNAPAAPAKPAAPAAEAPIFAAGDRVEKRVPMTRLRAKVAERLVEAQSAMAMLTTFNEVNMKPIMDLRSKYKDLFEKKHNGVRLGFMSFFVKAATEALKRFPGVNASIDGNDIVYHGYQDIGVAVSSDRGLVVPVLRNAEFMSLAEIEGGIANFGKKAKEGKLTIEDMTGGTFTISNGGVFGSLLSTPIVNPPQTAILGMHKIQERPMAVNGQVVILPMMYLALSYDHRLIDGKEAVSFLVAIKDLLEDPARLLLDV
References
External Links:
Resource Link
Genome ID: PA1586
Entrez Gene ID: 881930
NCBI Protein ID: 15596783
General Reference: PaperBLAST - Find papers about PA1586 and its homologs