5,10-methylenetetrahydrofolate reductase (PA0430)

Identification
Name: 5,10-methylenetetrahydrofolate reductase
Synonyms: Not Available
Gene Name: metF
Enzyme Class:
Biological Properties
General Function: Involved in methylenetetrahydrofolate reductase (NADPH) activity
Specific Function: 5-methyltetrahydrofolate + NAD(P)(+) = 5,10- methylenetetrahydrofolate + NAD(P)H
Cellular Location: Not Available
KEGG Pathways:
KEGG Reactions:
2 Thumb+Thumb+Thumb → Thumb+Thumb
2 Hydrogen ion + 5,10-Methylene-THF + NADH → 5-Methyltetrahydrofolic acid + NAD
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Thumb+Thumb ↔ Thumb+Thumb+Thumb
5-Methyltetrahydrofolic acid + NADP ↔ 5,10-Methylene-THF + NADPH + Hydrogen ion
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Thumb+Thumb ↔ Thumb+Thumb+Thumb
5-Methyltetrahydrofolic acid + NAD ↔ 5,10-Methylene-THF + NADH + Hydrogen ion
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Thumb+Thumb+Thumb ↔ Thumb+Thumb+Thumb+Thumb
5-Methyltetrahydrofolic acid + NAD + NADP ↔ 5,10-Methylene-THF + NADH + NADPH + Hydrogen ion
ReactionCard
SMPDB Reactions:
5,10-Methylene-THF+NADPH+Thumb+Thumb+Thumb → 5-Methyltetrahydrofolic acid+Thumb+Thumb
5,10-Methylene-THF + NADPH + Hydrogen ion + 5,10-Methylene-THF + NADPH → 5-Methyltetrahydrofolic acid + NADP + 5-Methyltetrahydrofolic acid
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PseudoCyc/BioCyc Reactions:
2 Thumb+Thumb+Thumb → Thumb+Thumb
2 Hydrogen ion + 5,10-Methylene-THF + NADH → 5-Methyltetrahydrofolic acid + NAD
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Thumb+Thumb ↔ Thumb+Thumb+Thumb
5-Methyltetrahydrofolic acid + NAD ↔ 5,10-Methylene-THF + NADH + Hydrogen ion
ReactionCard
Complex Reactions:
Thumb+NAD(P)(+) → Thumb+NAD(P)H
5-Methyltetrahydrofolic acid + NAD(P)(+) → 5,10-Methylene-THF + NAD(P)H
ReactionCard
Transports: Not Available
Metabolites:
PAMDB IDNameView
PAMDB0003535,10-Methylene-THFMetaboCard
PAMDB0003685-Methyltetrahydrofolic acidMetaboCard
PAMDB001633Hydrogen ionMetaboCard
PAMDB000197NADMetaboCard
PAMDB000398NADHMetaboCard
PAMDB000089NADPMetaboCard
PAMDB000615NADPHMetaboCard
GO Classification:
Function
catalytic activity
methylenetetrahydrofolate reductase (NADPH) activity
oxidoreductase activity
oxidoreductase activity, acting on the CH-NH group of donors
oxidoreductase activity, acting on the CH-NH group of donors, NAD or NADP as acceptor
Process
cellular amino acid and derivative metabolic process
cellular amino acid metabolic process
cellular metabolic process
metabolic process
methionine biosynthetic process
methionine metabolic process
oxidation reduction
sulfur amino acid biosynthetic process
sulfur amino acid metabolic process
Gene Properties
Locus tag: PA0430
Strand: -
Entrez Gene ID: 877765
Accession: NP_249121.1
GI: 15595627
Sequence start: 481196
Sequence End: 482068
Sequence Length: 872
Gene Sequence: 
>PA0430
GTGGTCGCGTCCAAGGAACCGATCATGAGTCAGAGCGAACGCCGTTTCAGCTTCGAGTTCTTCCCGGCGAAGACCGAGGCCGGCCATGAAAAGCTGTTGGCCACCGCCCGCAACCTGGCGGGCTACAAGCCCGACTTCTTCTCCTGCACCTACGGCGCCGGCGGATCCACCCGCGACCGCACGTTGAGTACCGTGCTGCAACTGGACGGCGAGGTGAAGGTGCCGACCGCGCCGCACCTGTCCTGTGTCGGCGACTCGAAAGCCGAGTTGCGCGAACTGCTCGGCCGCTACCGCGAGGCCGGCATCCGCCGCATCGTCGCCCTGCGCGGCGACCTGCCGTCGGGCATGGGCATGGCCAGCGGCGAACTGCGCTACGCCAACGAACTGGTGGACTTCATCCGCACCGAGACCGGCGACCACTTCCACATCGAGGTCGCCGCCTATCCGGAGGTCCACCCCCAGGCGCGCAGCTTCGAGGATGACCTGGCGAACTTCGTGCGCAAGGTGAAGGCCGGCGCCAGCAGCGCCATCACCCAGTACTTCTTCAACGCCGATGCCTATTTCTACTTCGTCGAGCGGGTCGCCAAGCTCGGCGTGGACATCCCGGTGGTCCCCGGCATCATGCCGATCACCAACTACTCCAAGCTGGCGCGCTTCTCCGACGCCTGCGGCGCCGAACTGCCGCGCTGGATCCGCAAGCAACTGGAAGCCTACGGCGACGACAGCCGCAGCATCCAGGCCTTCGGCGAGCAGGTCATCAGCGAGATGTGCGAACGCCTGCTGGAGGGCGGCGCACCGGGACTGCATTTCTATACTTTGAACCAGGCCGATCCGAGCCTGGCGATCTGGAAGAATCTCCAGCTGCCACGCTGA
Protein Properties
Protein Residues: 290
Protein Molecular Weight: 32.2 kDa
Protein Theoretical pI: 6.52
Hydropathicity (GRAVY score): -0.236
Charge at pH 7 (predicted): -1.68
Protein Sequence: 
>PA0430
MVASKEPIMSQSERRFSFEFFPAKTEAGHEKLLATARNLAGYKPDFFSCTYGAGGSTRDRTLSTVLQLDGEVKVPTAPHLSCVGDSKAELRELLGRYREAGIRRIVALRGDLPSGMGMASGELRYANELVDFIRTETGDHFHIEVAAYPEVHPQARSFEDDLANFVRKVKAGASSAITQYFFNADAYFYFVERVAKLGVDIPVVPGIMPITNYSKLARFSDACGAELPRWIRKQLEAYGDDSRSIQAFGEQVISEMCERLLEGGAPGLHFYTLNQADPSLAIWKNLQLPR
References
External Links:
Resource Link
Genome ID: PA0430
Entrez Gene ID: 877765
NCBI Protein ID: 15595627
General Reference: PaperBLAST - Find papers about PA0430 and its homologs