D-Alanine (PAMDB000333)
Enzymes
- General function:
- Involved in catalytic activity
- Specific function:
- Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal domain (cross-linking of the peptide subunits)
- Gene Name:
- mrcB
- Locus Tag:
- PA4700
- Molecular weight:
- 85.5 kDa
Reactions
| (GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala))(n)-diphosphoundecaprenol + GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol = (GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala))(n+1)-diphosphoundecaprenol + undecaprenyl diphosphate. |
- General function:
- Involved in ATP binding
- Specific function:
- Cell wall formation
- Gene Name:
- ddlB
- Locus Tag:
- PA4410
- Molecular weight:
- 34.4 kDa
Reactions
| ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. |
- General function:
- Involved in alanine racemase activity
- Specific function:
- Provides the D-alanine required for cell wall biosynthesis
- Gene Name:
- alr
- Locus Tag:
- PA4930
- Molecular weight:
- 38.3 kDa
Reactions
| L-alanine = D-alanine. |
- General function:
- Involved in D-amino-acid dehydrogenase activity
- Specific function:
- Oxidative deamination of D-amino acids
- Gene Name:
- dadA
- Locus Tag:
- PA5304
- Molecular weight:
- 47.1 kDa
Reactions
| A D-amino acid + H(2)O + acceptor = a 2-oxo acid + NH(3) + reduced acceptor. |
- General function:
- Involved in ATP binding
- Specific function:
- Cell wall formation
- Gene Name:
- ddlA
- Locus Tag:
- PA4201
- Molecular weight:
- 36.5 kDa
Reactions
| ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. |
- General function:
- Involved in catalytic activity
- Specific function:
- Interconversion of serine and glycine
- Gene Name:
- glyA
- Locus Tag:
- PA4602
- Molecular weight:
- 45.2 kDa
Reactions
| 5,10-methylenetetrahydrofolate + glycine + H(2)O = tetrahydrofolate + L-serine. |
- General function:
- Involved in penicillin binding
- Specific function:
- Cell wall formation. Essential for the formation of a septum of the murein sacculus. Synthesis of cross-linked peptidoglycan from the lipid intermediates
- Gene Name:
- ftsI
- Locus Tag:
- PA4418
- Molecular weight:
- 62.9 kDa
Reactions
| (GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala))(n)-diphosphoundecaprenol + GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol = (GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala))(n+1)-diphosphoundecaprenol + undecaprenyl diphosphate. |
- General function:
- Involved in alanine racemase activity
- Specific function:
- Isomerizes L-alanine to D-alanine which is then oxidized to pyruvate by dadA
- Gene Name:
- dadX
- Locus Tag:
- PA5302
- Molecular weight:
- 38.9 kDa
Reactions
| L-alanine = D-alanine. |
- General function:
- Involved in lyase activity
- Specific function:
- Catalyzes the cleavage of L-allo-threonine and L- threonine to glycine and acetaldehyde. L-threo-phenylserine and L- erythro-phenylserine are also good substrates
- Gene Name:
- ltaE
- Locus Tag:
- PA0902
- Molecular weight:
- 35.4 kDa
Reactions
| L-threonine = glycine + acetaldehyde. |
| L-allo-threonine = glycine + acetaldehyde. |
- General function:
- Defense mechanisms
- Specific function:
- Releases the terminal D-alanine residue from the cytoplasmic tetrapeptide recycling product L-Ala-gamma-D-Glu-meso- Dap-D-Ala. To a lesser extent, can also cleave D-Ala from murein derivatives containing the tetrapeptide, i.e. MurNAc-tetrapeptide, UDP-MurNAc-tetrapeptide, GlcNAc-MurNAc-tetrapeptide, and GlcNAc- anhMurNAc-tetrapeptide. Does not act on murein sacculi or cross- linked muropeptides. The tripeptides produced by the lcdA reaction can then be reused as peptidoglycan building blocks; lcdA is thereby involved in murein recycling. Is also essential for viability during stationary phase
- Gene Name:
- ldcA
- Locus Tag:
- PA1818
- Molecular weight:
- 82.8 kDa
Reactions
| GlcNAc-MurNAc-L-alanyl-gamma-D-glutamyl-meso-diaminopimelyl-D-alanine + H(2)O = GlcNAc-MurNAc-L-alanyl-gamma-D-glutamyl-meso-diaminopimelate + D-alanine. |
- General function:
- Involved in carboxypeptidase activity
- Specific function:
- Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors
- Gene Name:
- dacC
- Locus Tag:
- PA3999
- Molecular weight:
- 42.5 kDa
Reactions
| Preferential cleavage: (Ac)(2)-L-Lys-D-Ala-|-D-Ala. Also transpeptidation of peptidyl-alanyl moieties that are N-acyl substituents of D-alanine. |
Transporters
- General function:
- Involved in nucleotide binding
- Specific function:
- Probably part of a binding-protein-dependent transport system yecCS for an amino acid. Probably responsible for energy coupling to the transport system
- Gene Name:
- yecC
- Locus Tag:
- PA5152
- Molecular weight:
- 28.4 kDa