Peptidoglycan synthase ftsI (PA4418)

Identification
Name: Peptidoglycan synthase ftsI
Synonyms:
  • Penicillin-binding protein 3
  • PBP-3
  • Peptidoglycan glycosyltransferase 3
Gene Name: ftsI
Enzyme Class:
Biological Properties
General Function: Involved in penicillin binding
Specific Function: Cell wall formation. Essential for the formation of a septum of the murein sacculus. Synthesis of cross-linked peptidoglycan from the lipid intermediates
Cellular Location: Cell inner membrane; Single-pass membrane protein; Periplasmic side.
KEGG Pathways:
KEGG Reactions:
Thumb+[GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-g-D-Glu-A2pm-D-Ala-D-Ala)]n-diphosphoundecaprenol ↔ di-trans,poly-cis-Undecaprenyl diphosphate+[GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-g-D-Glu-A2pm-D-Ala-D-Ala)]n-diphosphoundecaprenol
Undecaprenyl-diphospho-N-acetylmuramoyl-(N-acetylglucosamine)-L-alanyl-D-glutamyl-meso-2,6-diaminopimeloyl-D-alanyl-D-alanine + [GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-g-D-Glu-A2pm-D-Ala-D-Ala)]n-diphosphoundecaprenol ↔ di-trans,poly-cis-Undecaprenyl diphosphate + [GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-g-D-Glu-A2pm-D-Ala-D-Ala)]n-diphosphoundecaprenol
ReactionCard
[GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-g-D-Glu-L-Lys-D-Ala-D-Ala)]n-diphosphoundecaprenol+Thumb ↔ Thumb
[GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-g-D-Glu-L-Lys-D-Ala-D-Ala)]n-diphosphoundecaprenol + Undecaprenyl-diphospho-N-acetylmuramoyl-(N-acetylglucosamine)-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine ↔ Undecaprenyl diphosphate
ReactionCard
SMPDB Reactions:
a peptidoglycan dimer (meso-diaminopimelate containing) → Thumb+a peptidoglycan with D,D cross-links (meso-diaminopimelate containing)
a peptidoglycan dimer (meso-diaminopimelate containing) → D-Alanine + a peptidoglycan with D,D cross-links (meso-diaminopimelate containing)
ReactionCard
PseudoCyc/BioCyc Reactions:
a peptidoglycan dimer (meso-diaminopimelate containing)+Thumb → a peptidoglycan with D,D cross-links (meso-diaminopimelate containing)+Thumb+Thumb
a peptidoglycan dimer (meso-diaminopimelate containing) + Water → a peptidoglycan with D,D cross-links (meso-diaminopimelate containing) + Undecaprenyl diphosphate + D-Alanine
ReactionCard
peptidoglycan D-alanyl-DAP crosslink+Thumb ↔ peptidoglycan tetrapeptide, glycan chain 2+peptidoglycan tetrapeptide, glycan chain 1
peptidoglycan D-alanyl-DAP crosslink + Water ↔ peptidoglycan tetrapeptide, glycan chain 2 + peptidoglycan tetrapeptide, glycan chain 1
ReactionCard
Complex Reactions:
two linked disacharide pentapeptide murein units (uncrosslinked, middle of chain) → Thumb+two disacharide linked murein units, pentapeptide crosslinked tetrapeptide (A2pm->D-ala) (middle of chain)
two linked disacharide pentapeptide murein units (uncrosslinked, middle of chain) → D-Alanine + two disacharide linked murein units, pentapeptide crosslinked tetrapeptide (A2pm->D-ala) (middle of chain)
ReactionCard
three linked disacharide pentapeptide murein units (uncrosslinked, middle of chain) → 2 Thumb+three disacharide linked murein units (pentapeptide crosslinked tetrapeptide (A2pm->D-ala) tetrapeptide corsslinked tetrapeptide (A2pm->D-ala)) (middle of chain)
three linked disacharide pentapeptide murein units (uncrosslinked, middle of chain) → 2 D-Alanine + three disacharide linked murein units (pentapeptide crosslinked tetrapeptide (A2pm->D-ala) tetrapeptide corsslinked tetrapeptide (A2pm->D-ala)) (middle of chain)
ReactionCard
(GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala))(n)-diphosphoundecaprenol+Thumb → (GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala))(n+1)-diphosphoundecaprenol+Thumb
(GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala))(n)-diphosphoundecaprenol + GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol → (GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala))(n+1)-diphosphoundecaprenol + Undecaprenyl diphosphate
ReactionCard
Transports: Not Available
Metabolites:
PAMDB IDNameView
PAMDB000333D-AlanineMetaboCard
PAMDB003414GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenolMetaboCard
PAMDB000394Undecaprenyl diphosphateMetaboCard
PAMDB001058Undecaprenyl-diphospho-N-acetylmuramoyl-(N-acetylglucosamine)-L-alanyl-D-glutamyl-meso-2,6-diaminopimeloyl-D-alanyl-D-alanineMetaboCard
PAMDB000142WaterMetaboCard
GO Classification:
Function
binding
drug binding
penicillin binding
Process
cell wall biogenesis
cell wall organization or biogenesis
cellular cell wall organization or biogenesis
cellular component organization or biogenesis
peptidoglycan-based cell wall biogenesis
Gene Properties
Locus tag: PA4418
Strand: -
Entrez Gene ID: 881247
Accession: NP_253108.1
GI: 15599614
Sequence start: 4952604
Sequence End: 4954343
Sequence Length: 1739
Gene Sequence: 
>PA4418
ATGAAACTGAATTATTTCCAGGGCGCCCTCTACCCATGGCGGTTCTGCGTGATCGTCGGCCTGCTGCTGGCGATGGTCGGCGCCATCGTCTGGCGAATCGTCGACCTGCACGTGATCGACCATGACTTCCTCAAGGGCCAGGGCGACGCGCGTAGCGTGCGGCATATCGCCATCCCTGCGCACCGGGGCCTGATCACTGACCGCAACGGCGAACCGCTGGCGGTGAGCACCCCGGTCACCACCCTGTGGGCCAACCCCAAGGAGCTGATGACCGCCAAGGAACGCTGGCCGCAACTGGCGGCGGCGCTCGGGCAGGATACCAAGCTGTTCGCCGACCGCATCGAGCAGAACGCCGAGCGCGAGTTCATCTATCTGGTCCGTGGGCTGACCCCGGAGCAGGGCGAAGGCGTGATCGCCCTGAAGGTGCCCGGCGTGTACTCCATCGAGGAGTTTCGGCGTTTCTACCCGGCTGGCGAAGTGGTGGCCCATGCGGTCGGCTTTACCGATGTCGACGACCGCGGTCGCGAAGGTATCGAGCTGGCTTTCGACGAATGGCTGGCCGGCGTGCCGGGCAAGCGCCAGGTGCTCAAGGATCGCCGTGGCCGCGTGATCAAGGACGTGCAGGTCACCAAGAATGCCAAACCGGGCAAGACCCTTGCGCTGTCCATCGACCTGCGCCTGCAGTACCTGGCTCATCGCGAACTGCGCAACGCTCTGCTGGAAAACGGCGCCAAGGCCGGCAGCTTGGTGATCATGGACGTGAAGACCGGGGAGATCCTGGCCATGACCAACCAGCCCACCTACAACCCGAACAATCGTCGTAACCTGCAGCCGGCGGCCATGCGCAACCGGGCGATGATCGACGTGTTCGAGCCGGGCTCGACGGTCAAGCCGTTCTCGATGAGCGCGGCGCTGGCCAGCGGGCGCTGGAAACCCAGCGATATCGTCGACGTCTACCCGGGCACCCTGCAGATCGGCCGCTACACCATTCGCGACGTATCGCGCAATTCGCGGCAACTCGATCTCACCGGCATCCTGATCAAGTCGAGCAACGTCGGCATCAGCAAGATCGCCTTCGACATCGGCGCCGAATCCATCTACTCGGTCATGCAACAGGTCGGTCTCGGGCAGGACACGGGGTTGGGCTTCCCCGGCGAGCGCGTCGGCAACCTGCCCAACCACCGCAAGTGGCCGAAGGCGGAAACCGCGACCCTGGCCTACGGCTACGGTCTCTCGGTAACCGCGATCCAGTTGGCGCATGCCTATGCGGCCCTGGCCAACGACGGCAAGAGCGTGCCGCTGAGCATGACCCGAGTCGACCGCGTGCCGGATGGTGTGCAGGTGATCTCGCCTGAAGTGGCTTCCACCGTGCAGGGCATGCTGCAACAAGTGGTCGAGGCCCAGGGCGGGGTGTTCCGCGCCCAGGTGCCGGGTTACCACGCCGCCGGCAAGAGCGGGACCGCGCGCAAGGTCTCGGTCGGCACCAAGGGCTACCGGGAAAACGCCTATCGCTCGCTGTTCGCCGGTTTCGCCCCGGCCACCGATCCGCGCATCGCGATGGTCGTGGTGATCGACGAGCCGAGCAAGGCGGGCTACTTCGGCGGCCTGGTGTCGGCGCCGGTGTTCAGTAAGGTCATGGCTGGCGCGCTGCGCCTGATGAACGTGCCGCCGGATAACCTGCCGACGGCCACCGAACAGCAGCAGGTCAATGCTGCGCCCGCAAAAGGAGGGCGTGGCTGA
Protein Properties
Protein Residues: 579
Protein Molecular Weight: 62.9 kDa
Protein Theoretical pI: 10.04
Hydropathicity (GRAVY score): -0.125
Charge at pH 7 (predicted): 16.14
Protein Sequence: 
>PA4418
MKLNYFQGALYPWRFCVIVGLLLAMVGAIVWRIVDLHVIDHDFLKGQGDARSVRHIAIPAHRGLITDRNGEPLAVSTPVTTLWANPKELMTAKERWPQLAAALGQDTKLFADRIEQNAEREFIYLVRGLTPEQGEGVIALKVPGVYSIEEFRRFYPAGEVVAHAVGFTDVDDRGREGIELAFDEWLAGVPGKRQVLKDRRGRVIKDVQVTKNAKPGKTLALSIDLRLQYLAHRELRNALLENGAKAGSLVIMDVKTGEILAMTNQPTYNPNNRRNLQPAAMRNRAMIDVFEPGSTVKPFSMSAALASGRWKPSDIVDVYPGTLQIGRYTIRDVSRNSRQLDLTGILIKSSNVGISKIAFDIGAESIYSVMQQVGLGQDTGLGFPGERVGNLPNHRKWPKAETATLAYGYGLSVTAIQLAHAYAALANDGKSVPLSMTRVDRVPDGVQVISPEVASTVQGMLQQVVEAQGGVFRAQVPGYHAAGKSGTARKVSVGTKGYRENAYRSLFAGFAPATDPRIAMVVVIDEPSKAGYFGGLVSAPVFSKVMAGALRLMNVPPDNLPTATEQQQVNAAPAKGGRG
References
External Links:
Resource Link
Genome ID: PA4418
Entrez Gene ID: 881247
NCBI Protein ID: 15599614
General Reference: PaperBLAST - Find papers about PA4418 and its homologs