Penicillin-binding protein 1B (PA4700)

Identification
Name: Penicillin-binding protein 1B
Synonyms:
  • PBP-1b
  • PBP1b
  • Murein polymerase
  • Penicillin-insensitive transglycosylase
  • Peptidoglycan TGase
  • Peptidoglycan glycosyltransferase
  • Penicillin-sensitive transpeptidase
  • DD-transpeptidase
Gene Name: mrcB
Enzyme Class:
Biological Properties
General Function: Involved in catalytic activity
Specific Function: Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal domain (cross-linking of the peptide subunits)
Cellular Location: Cell inner membrane; Single-pass type II membrane protein
KEGG Pathways:
KEGG Reactions:
Thumb+[GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-g-D-Glu-A2pm-D-Ala-D-Ala)]n-diphosphoundecaprenol ↔ di-trans,poly-cis-Undecaprenyl diphosphate+[GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-g-D-Glu-A2pm-D-Ala-D-Ala)]n-diphosphoundecaprenol
Undecaprenyl-diphospho-N-acetylmuramoyl-(N-acetylglucosamine)-L-alanyl-D-glutamyl-meso-2,6-diaminopimeloyl-D-alanyl-D-alanine + [GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-g-D-Glu-A2pm-D-Ala-D-Ala)]n-diphosphoundecaprenol ↔ di-trans,poly-cis-Undecaprenyl diphosphate + [GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-g-D-Glu-A2pm-D-Ala-D-Ala)]n-diphosphoundecaprenol
ReactionCard
[GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-g-D-Glu-L-Lys-D-Ala-D-Ala)]n-diphosphoundecaprenol+Thumb ↔ Thumb
[GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-g-D-Glu-L-Lys-D-Ala-D-Ala)]n-diphosphoundecaprenol + Undecaprenyl-diphospho-N-acetylmuramoyl-(N-acetylglucosamine)-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine ↔ Undecaprenyl diphosphate
ReactionCard
SMPDB Reactions:
2 Thumb → Thumb+Thumb+ a peptidoglycan dimer (meso-diaminopimelate containing)
2 lipid II(A) → Undecaprenyl diphosphate + Hydrogen ion + a peptidoglycan dimer (meso-diaminopimelate containing)
ReactionCard
a peptidoglycan dimer (meso-diaminopimelate containing) → Thumb+a peptidoglycan with D,D cross-links (meso-diaminopimelate containing)
a peptidoglycan dimer (meso-diaminopimelate containing) → D-Alanine + a peptidoglycan with D,D cross-links (meso-diaminopimelate containing)
ReactionCard
2 Thumb → a peptidoglycan dimer (meso-diaminopimelate containing)+Thumb+Thumb
2 Undecaprenyl-diphospho-N-acetylmuramoyl-(N-acetylglucosamine)-L-alanyl-D-glutaminyl-meso-2,6-diaminopimeloyl-D-alanyl-D-alanine → a peptidoglycan dimer (meso-diaminopimelate containing) + Undecaprenyl diphosphate + Hydrogen ion
ReactionCard
PseudoCyc/BioCyc Reactions:
two linked disacharide pentapeptide murein units (uncrosslinked, middle of chain) → Thumb+two disacharide linked murein units, pentapeptide crosslinked tetrapeptide (A2pm->D-ala) (middle of chain)
two linked disacharide pentapeptide murein units (uncrosslinked, middle of chain) → D-Alanine + two disacharide linked murein units, pentapeptide crosslinked tetrapeptide (A2pm->D-ala) (middle of chain)
ReactionCard
three linked disacharide pentapeptide murein units (uncrosslinked, middle of chain) → 2 Thumb+three disacharide linked murein units (pentapeptide crosslinked tetrapeptide (A2pm->D-ala) tetrapeptide corsslinked tetrapeptide (A2pm->D-ala)) (middle of chain)
three linked disacharide pentapeptide murein units (uncrosslinked, middle of chain) → 2 D-Alanine + three disacharide linked murein units (pentapeptide crosslinked tetrapeptide (A2pm->D-ala) tetrapeptide corsslinked tetrapeptide (A2pm->D-ala)) (middle of chain)
ReactionCard
two linked disacharide pentapeptide murein units (uncrosslinked, middle of chain) → Thumb+two disacharide linked murein units, pentapeptide corsslinked tripeptide (A2pm->A2pm) (middle of chain)
two linked disacharide pentapeptide murein units (uncrosslinked, middle of chain) → D-Alanyl-D-alanine + two disacharide linked murein units, pentapeptide corsslinked tripeptide (A2pm->A2pm) (middle of chain)
ReactionCard
2 Thumb → Thumb+Thumb+ a peptidoglycan dimer (meso-diaminopimelate containing)
2 lipid II(A) → Undecaprenyl diphosphate + Hydrogen ion + a peptidoglycan dimer (meso-diaminopimelate containing)
ReactionCard
a peptidoglycan dimer (meso-diaminopimelate containing) → Thumb+a peptidoglycan with D,D cross-links (meso-diaminopimelate containing)
a peptidoglycan dimer (meso-diaminopimelate containing) → D-Alanine + a peptidoglycan with D,D cross-links (meso-diaminopimelate containing)
ReactionCard
Thumb+[GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-g-D-Glu-A2pm-D-Ala-D-Ala)]n-diphosphoundecaprenol ↔ di-trans,poly-cis-Undecaprenyl diphosphate+[GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-g-D-Glu-A2pm-D-Ala-D-Ala)]n-diphosphoundecaprenol
Undecaprenyl-diphospho-N-acetylmuramoyl-(N-acetylglucosamine)-L-alanyl-D-glutamyl-meso-2,6-diaminopimeloyl-D-alanyl-D-alanine + [GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-g-D-Glu-A2pm-D-Ala-D-Ala)]n-diphosphoundecaprenol ↔ di-trans,poly-cis-Undecaprenyl diphosphate + [GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-g-D-Glu-A2pm-D-Ala-D-Ala)]n-diphosphoundecaprenol
ReactionCard
(GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala))(n)-diphosphoundecaprenol+Thumb → (GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala))(n+1)-diphosphoundecaprenol+Thumb
(GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala))(n)-diphosphoundecaprenol + GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol → (GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala))(n+1)-diphosphoundecaprenol + Undecaprenyl diphosphate
ReactionCard
(GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala))(n)-diphosphoundecaprenol+Thumb → (GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala))(n+1)-diphosphoundecaprenol+Thumb
(GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala))(n)-diphosphoundecaprenol + GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol → (GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala))(n+1)-diphosphoundecaprenol + Undecaprenyl diphosphate
ReactionCard
[GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-g-D-Glu-L-Lys-D-Ala-D-Ala)]n-diphosphoundecaprenol+Thumb ↔ Thumb
[GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-g-D-Glu-L-Lys-D-Ala-D-Ala)]n-diphosphoundecaprenol + Undecaprenyl-diphospho-N-acetylmuramoyl-(N-acetylglucosamine)-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine ↔ Undecaprenyl diphosphate
ReactionCard
Complex Reactions:
Thumb+two linked disacharide pentapeptide murein units (uncrosslinked, middle of chain) → Thumb+Thumb+three linked disacharide pentapeptide murein units (uncrosslinked, middle of chain)
Undecaprenyl-diphospho-N-acetylmuramoyl-(N-acetylglucosamine)-L-alanyl-D-glutamyl-meso-2,6-diaminopimeloyl-D-alanyl-D-alanine + two linked disacharide pentapeptide murein units (uncrosslinked, middle of chain) → Hydrogen ion + Undecaprenyl diphosphate + three linked disacharide pentapeptide murein units (uncrosslinked, middle of chain)
ReactionCard
2 Thumb → 2 Thumb+2 Thumb+two linked disacharide pentapeptide murein units (uncrosslinked, middle of chain)
2 Undecaprenyl-diphospho-N-acetylmuramoyl-(N-acetylglucosamine)-L-alanyl-D-glutamyl-meso-2,6-diaminopimeloyl-D-alanyl-D-alanine → 2 Hydrogen ion + 2 Undecaprenyl diphosphate + two linked disacharide pentapeptide murein units (uncrosslinked, middle of chain)
ReactionCard
two linked disacharide pentapeptide murein units (uncrosslinked, middle of chain) → Thumb+two disacharide linked murein units, pentapeptide crosslinked tetrapeptide (A2pm->D-ala) (middle of chain)
two linked disacharide pentapeptide murein units (uncrosslinked, middle of chain) → D-Alanine + two disacharide linked murein units, pentapeptide crosslinked tetrapeptide (A2pm->D-ala) (middle of chain)
ReactionCard
three linked disacharide pentapeptide murein units (uncrosslinked, middle of chain) → 2 Thumb+three disacharide linked murein units (pentapeptide crosslinked tetrapeptide (A2pm->D-ala) tetrapeptide corsslinked tetrapeptide (A2pm->D-ala)) (middle of chain)
three linked disacharide pentapeptide murein units (uncrosslinked, middle of chain) → 2 D-Alanine + three disacharide linked murein units (pentapeptide crosslinked tetrapeptide (A2pm->D-ala) tetrapeptide corsslinked tetrapeptide (A2pm->D-ala)) (middle of chain)
ReactionCard
two linked disacharide pentapeptide murein units (uncrosslinked, middle of chain) → Thumb+two disacharide linked murein units, pentapeptide corsslinked tripeptide (A2pm->A2pm) (middle of chain)
two linked disacharide pentapeptide murein units (uncrosslinked, middle of chain) → D-Alanyl-D-alanine + two disacharide linked murein units, pentapeptide corsslinked tripeptide (A2pm->A2pm) (middle of chain)
ReactionCard
(GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala))(n)-diphosphoundecaprenol+Thumb → (GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala))(n+1)-diphosphoundecaprenol+Thumb
(GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala))(n)-diphosphoundecaprenol + GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol → (GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala))(n+1)-diphosphoundecaprenol + Undecaprenyl diphosphate
ReactionCard
(GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala))(n)-diphosphoundecaprenol+Thumb → (GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala))(n+1)-diphosphoundecaprenol+Thumb
(GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala))(n)-diphosphoundecaprenol + GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol → (GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala))(n+1)-diphosphoundecaprenol + Undecaprenyl diphosphate
ReactionCard
Transports: Not Available
Metabolites:
PAMDB IDNameView
PAMDB000333D-AlanineMetaboCard
PAMDB000504D-Alanyl-D-alanineMetaboCard
PAMDB003414GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenolMetaboCard
PAMDB001633Hydrogen ionMetaboCard
PAMDB003413lipid II(A)MetaboCard
PAMDB000394Undecaprenyl diphosphateMetaboCard
PAMDB001056Undecaprenyl-diphospho-N-acetylmuramoyl-(N-acetylglucosamine)-L-alanyl-D-glutaminyl-meso-2,6-diaminopimeloyl-D-alanyl-D-alanineMetaboCard
PAMDB001058Undecaprenyl-diphospho-N-acetylmuramoyl-(N-acetylglucosamine)-L-alanyl-D-glutamyl-meso-2,6-diaminopimeloyl-D-alanyl-D-alanineMetaboCard
GO Classification:
Component
cell part
cell wall
external encapsulating structure
peptidoglycan-based cell wall
Function
binding
catalytic activity
drug binding
hydrolase activity
penicillin binding
peptidase activity
peptidoglycan glycosyltransferase activity
transferase activity
transferase activity, transferring glycosyl groups
transferase activity, transferring hexosyl groups
Process
aminoglycan metabolic process
carbohydrate metabolic process
cell wall biogenesis
cell wall organization or biogenesis
cellular cell wall organization or biogenesis
cellular component organization or biogenesis
glycosaminoglycan metabolic process
metabolic process
peptidoglycan biosynthetic process
peptidoglycan metabolic process
peptidoglycan-based cell wall biogenesis
polysaccharide metabolic process
primary metabolic process
response to antibiotic
response to chemical stimulus
response to stimulus
Gene Properties
Locus tag: PA4700
Strand: -
Entrez Gene ID: 881511
Accession: NP_253388.1
GI: 15599894
Sequence start: 5277968
Sequence End: 5280292
Sequence Length: 2324
Gene Sequence: 
>PA4700
ATGACGCGTCCCCGATCCCCCCGTTCCCGTAACAGCAAAGCACGCCCGGCACCCGGCCTGAACAAATGGCTCAGCTGGGCTCTCAAGCTCGGCCTCGTCGGCCTGGTGCTCCTCGCCGGCTTCGCCATCTATCTCGATGCCGTGGTGCAGGAGAAATTCTCCGGCCGGCGCTGGACCATCCCGGCGAAGGTCTATGCCCGCCCGCTGGAGCTGTTCAACGGCCTGAAGCTGAGCAGGGAAGACTTCCTGCGCGAACTCGACGCCCTCGGCTACCGTCGCGAGCCCAGCGTGAGCGGCCCTGGAACCGTATCGGTGGCGGCCAGCGCGGTCGAACTCAATACCCGTGGCTTCCAGTTCTACGAAGGCGCGGAACCGGCCCAGCGCGTGCGCGTGCGCTTCAACGGCAACTACGTCAGCGGGCTGAGCCAGGCCAACGGCAAGGAACTGGCGGTGGCGCGCCTTGAGCCGCTGCTGATCGGCGGCTTGTATCCGGCGCACCATGAGGATCGCATCCTGGTCAAGCTCGACCAGGTGCCGACCTACCTGATCGACACCCTGGTCGCGGTCGAGGATCGCGACTTCTGGAACCACCATGGTGTGTCGCTGAAATCCGTGGCGCGGGCGGTGTGGGTCAACACCACGGCCGGCCAGTTGCGCCAGGGCGGCAGCACCCTGACCCAGCAGTTGGTGAAGAACTTCTTCCTGAGCAATGAGCGCAGCCTGAGCCGCAAGATCAACGAAGCGATGATGGCGGTGCTGCTGGAACTGCACTACGACAAGCGCGACATCCTCGAGTCCTATCTCAACGAGGTGTTCCTCGGCCAGGATGGGCAACGAGCGATCCATGGCTTCGGCCTGGCCAGCCAGTATTTCTTCAGCCAGCCGTTGGCCGAGCTCAAGCTCGACCAGGTGGCGCTGCTGGTCGGCATGGTCAAGGGGCCGTCCTACTTCAACCCGCGGCGTTACCCGGATCGTGCGCTGGCGCGGCGCAACCTGGTGCTCGACGTGCTCGCCGAGCAGGGCGTGGCGACCCAGCAGGAAGTCGACGCCGCCAAGCTGCGCCCGCTCGGCGTGACCCGGCAGGGCAGCATGGCCGACAGCTCCTATCCGGCCTTCCTCGACCTGGTCAAGCGCCAGTTGCGCCAGGACTACCGCGACGAGGACCTGACTGAGGAAGGGCTGCGCATCTTCACCAGCTTCGATCCGATCCTGCAGGAGAAGGCCGAGACCTCCGTCAACGAAACGCTCAAGCGTCTCAGTGGGCGCAAGGGGGTCGACCAGGTCGAGGCGGCGATGGTCGTGACCAATCCGGAAACCGGTGAGATCCAGGCCCTGATCGGCAGTCGCGACCCGCGCTTCGCCGGCTTCAACCGGGCCCTGGACGCGGTGCGGCCGATCGGTTCGCTGATCAAGCCGGCGGTCTACCTGACCGCTCTCGAGCGGCCGAGCAAGTACACCCTGACCACCTGGGTACAGGACGAGCCCTTCGCGGTGAAAGGCCAGGACGGCCAGGTCTGGCGACCGCAGAACTACGACCGGCGCTCGCATGGCACGATCTTCCTGTACCAGGGCCTGGCAAACTCCTACAACCTGTCCACCGCCAAGCTCGGCCTCGACGTCGGTGTGCCCAACGTGCTGCAGACCGTCGCCCGGCTGGGTATCAACCGTGACTGGCCGGCCTATCCGTCGATGCTGCTGGGCGCCGGTTCGCTCAGTCCGATGGAGGTCGCCACCATGTACCAGACCATCGCCAGCGGCGGCTTCAACACGCCGTTGCGGGGGATCCGCAGCGTCCTCACTGCCGACGGCCAGCCGCTCAAGCGCTATCCGTTCCAGGTCGAACAGCGCTTCGACTCGGGCGCCGTCTACCTGGTGCAGAACGCCATGCAGCGGGTCATGCGCGAAGGCACCGGGCGTTCGGTGTACAGTCAATTGCCGTCGTCGCTGACCCTCGCCGGCAAGACCGGCACCAGCAACGACTCGCGCGATAGCTGGTTCTCCGGTTTCGGCGGCGACCTCCAGGCTGTGGTCTGGCTCGGTCGCGACGACAACGGCAAGACGCCGCTTACCGGCGCCACCGGTGCCTTGCAGGTGTGGGCCAGCTTCATGCGCAAGGCGCATCCGCAATCGCTGGAAATGCCGATGCCGGAGAACGTGGTGATGGCCTGGGTCGACGCGCAGACCGGGCAGGGCTCGGCGGCGGATTGCCCGAATGCCGTACAGATGCCCTATATTCGTGGCAGCGAGCCCGCCCAGGGCCCAGGCTGCGGGAGCCAGAACCCCGCCGGCGAGGTGATGGACTGGGTACGTGGCTGGCTGAATTGA
Protein Properties
Protein Residues: 774
Protein Molecular Weight: 85.5 kDa
Protein Theoretical pI: 9.57
Hydropathicity (GRAVY score): -0.282
Charge at pH 7 (predicted): 11.89
Protein Sequence: 
>PA4700
MTRPRSPRSRNSKARPAPGLNKWLSWALKLGLVGLVLLAGFAIYLDAVVQEKFSGRRWTIPAKVYARPLELFNGLKLSREDFLRELDALGYRREPSVSGPGTVSVAASAVELNTRGFQFYEGAEPAQRVRVRFNGNYVSGLSQANGKELAVARLEPLLIGGLYPAHHEDRILVKLDQVPTYLIDTLVAVEDRDFWNHHGVSLKSVARAVWVNTTAGQLRQGGSTLTQQLVKNFFLSNERSLSRKINEAMMAVLLELHYDKRDILESYLNEVFLGQDGQRAIHGFGLASQYFFSQPLAELKLDQVALLVGMVKGPSYFNPRRYPDRALARRNLVLDVLAEQGVATQQEVDAAKLRPLGVTRQGSMADSSYPAFLDLVKRQLRQDYRDEDLTEEGLRIFTSFDPILQEKAETSVNETLKRLSGRKGVDQVEAAMVVTNPETGEIQALIGSRDPRFAGFNRALDAVRPIGSLIKPAVYLTALERPSKYTLTTWVQDEPFAVKGQDGQVWRPQNYDRRSHGTIFLYQGLANSYNLSTAKLGLDVGVPNVLQTVARLGINRDWPAYPSMLLGAGSLSPMEVATMYQTIASGGFNTPLRGIRSVLTADGQPLKRYPFQVEQRFDSGAVYLVQNAMQRVMREGTGRSVYSQLPSSLTLAGKTGTSNDSRDSWFSGFGGDLQAVVWLGRDDNGKTPLTGATGALQVWASFMRKAHPQSLEMPMPENVVMAWVDAQTGQGSAADCPNAVQMPYIRGSEPAQGPGCGSQNPAGEVMDWVRGWLN
References
External Links:
Resource Link
Genome ID: PA4700
Entrez Gene ID: 881511
NCBI Protein ID: 15599894
General Reference: PaperBLAST - Find papers about PA4700 and its homologs