Polyribonucleotide nucleotidyltransferase (PA4740)

Identification
Name: Polyribonucleotide nucleotidyltransferase
Synonyms:
  • Polynucleotide phosphorylase
  • PNPase
Gene Name: pnp
Enzyme Class:
Biological Properties
General Function: Involved in RNA binding
Specific Function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction
Cellular Location: Cytoplasm.
KEGG Pathways:
KEGG Reactions:
RNA+Thumb ↔ RNA+Thumb
RNA + Phosphate ↔ RNA + ADP
ReactionCard
RNA+Thumb ↔ RNA+Thumb
RNA + Phosphate ↔ RNA + Uridine 5'-diphosphate
ReactionCard
RNA+Thumb ↔ RNA+Thumb
RNA + Phosphate ↔ RNA + Guanosine diphosphate
ReactionCard
RNA+Thumb ↔ RNA+Thumb
RNA + Phosphate ↔ RNA + CDP
ReactionCard
RNA+Thumb ↔ NDP
RNA + Phosphate ↔ NDP
ReactionCard
SMPDB Reactions: Not Available
PseudoCyc/BioCyc Reactions:
RNA+Thumb ↔ RNA+Thumb
RNA + Phosphate ↔ RNA + ADP
ReactionCard
RNA+Thumb ↔ RNA+Thumb
RNA + Phosphate ↔ RNA + Uridine 5'-diphosphate
ReactionCard
RNA+Thumb ↔ RNA+Thumb
RNA + Phosphate ↔ RNA + Guanosine diphosphate
ReactionCard
RNA+Thumb ↔ RNA+Thumb
RNA + Phosphate ↔ RNA + CDP
ReactionCard
RNA(n+1)+Thumb → RNA(n)+a nucleoside diphosphate
RNA(n+1) + Inorganic phosphate → RNA(n) + a nucleoside diphosphate
ReactionCard
RNA+Thumb ↔ NDP
RNA + Phosphate ↔ NDP
ReactionCard
Thumb
a single-stranded RNA + phosphate = a single-stranded RNA + a nucleoside diphosphate
ReactionCard
Complex Reactions:
RNA(n+1)+Thumb → RNA(n)+a nucleoside diphosphate
RNA(n+1) + Inorganic phosphate → RNA(n) + a nucleoside diphosphate
ReactionCard
Transports: Not Available
Metabolites:
PAMDB IDNameView
PAMDB000345ADPMetaboCard
PAMDB000415CDPMetaboCard
PAMDB000296Guanosine diphosphateMetaboCard
PAMDB001776Inorganic phosphateMetaboCard
PAMDB000383PhosphateMetaboCard
PAMDB000123Uridine 5'-diphosphateMetaboCard
GO Classification:
Component
intracellular membrane-bounded organelle
membrane-bounded organelle
mitochondrion
organelle
Function
3'-5' exonuclease activity
3'-5'-exoribonuclease activity
binding
catalytic activity
exonuclease activity
hydrolase activity
hydrolase activity, acting on ester bonds
nuclease activity
nucleic acid binding
nucleotidyltransferase activity
polyribonucleotide nucleotidyltransferase activity
RNA binding
transferase activity
transferase activity, transferring phosphorus-containing groups
Process
cellular macromolecule metabolic process
macromolecule metabolic process
metabolic process
mRNA catabolic process
RNA catabolic process
RNA metabolic process
RNA processing
Gene Properties
Locus tag: PA4740
Strand: -
Entrez Gene ID: 881671
Accession: NP_253428.1
GI: 15599934
Sequence start: 5323374
Sequence End: 5325479
Sequence Length: 2105
Gene Sequence: 
>PA4740
GTGAACCCGGTAACCAAGCAATTCCAGTTCGGTCAGTCGACCGTCACTCTCGAGACCGGCCGTATCGCTCGCCAGGCCACCGGCGCCGTACTGGTCACCATGGACGACGTCAGCGTGCTGGTCACCGTGGTCGGCGCCAAGTCGCCGGCCGAAGGCCGCGACTTCTTCCCGCTGTCCGTGCACTACCAGGAAAAGACCTACGCCGCAGGCCGTATCCCCGGTGGTTTCTTCAAGCGTGAAGGACGTCCTTCCGAGAAGGAAACCCTGACCTCGCGCCTGATCGACCGTCCGATCCGTCCGCTGTTCCCCGAAGGTTTCATGAATGAAGTGCAGGTCGTCTGCACCGTCGTCTCCACCAACAAGAAGTCCGATCCGGACATCGCCGCGATGATCGGCACCTCCGCCGCCCTGGCGATCTCCGGCATTCCGTTCGCCGGCCCGATCGGCGCCGCCCGCGTCGGTTTCCATCCGGAAATCGGCTACATCCTCAACCCCACCTACGAGCAACTGCAGAGTTCCAGCCTCGACATGGTCGTGGCCGGTACCGAAGACGCCGTGCTGATGGTGGAGTCCGAGGCCGACGAACTGACCGAAGACCAGATGCTCGGTGCCGTGCTGTTCGCCCATGACGAGTTCCAGGCGGTGATCCGTGCCGTCAAGGAGCTGGCCGCCGAAGCTGGCAAGCCGGCCTGGGACTGGAAAGCCCCGGCGGAAAACACCGTGCTGGTCAACGCCATCAAGGCAGAGCTGGGCGAAGCGATCTCCCAGGCCTATACCATCACCATCAAGCAGGACCGCTACAACCGTCTGGGCGAGCTGCGCGACCAGGCCGTGGCCCTGTTCGCCGGCGAGGAAGAGGGCAAGTTCCCGGCTTCCGAGGTCAAGGACGTATTCGGCCTGCTGGAATACCGCACCGTTCGCGAGAACATCGTCAACGGCAAGCCGCGTATCGACGGTCGTGACACCCGTACCGTGCGTCCGCTGCGCATCGAAGTCGGCGTGCTCGGCAAGACCCACGGTTCCGCGCTGTTCACCCGTGGCGAAACCCAGGCCCTGGTCGTTGCGACCCTCGGCACCGCGCGTGATGCCCAGCTGCTGGATACCCTGGAAGGCGAGCGCAAGGACGCCTTCATGCTGCACTACAACTTCCCGCCGTTCTCGGTAGGCGAGTGCGGCCGCATGGGCAGCCCGGGTCGTCGTGAAATCGGCCATGGCCGTCTGGCCCGTCGTGGCGTCGCCGCCATGCTGCCGACCCAGGACGAGTTCCCCTACACCATTCGCGTGGTTTCCGAGATCACCGAGTCCAACGGTTCCAGCTCGATGGCCTCCGTGTGCGGCGCCTCCCTGGCGCTGATGGATGCCGGTGTGCCGGTCAAGGCGCCGGTCGCCGGTATCGCCATGGGCCTGGTCAAGGAAGGCGAGAAGTTCGCCGTCCTGACCGACATCCTCGGTGACGAGGACCACCTGGGCGACATGGACTTCAAGGTTGCCGGTACCGACAAGGGCGTCACCGCCCTGCAGATGGACATCAAGATCAACGGTATCACCGAAGAGATCATGGAGATCGCCCTGGGCCAGGCCCTGGAAGCTCGCCTGAACATCCTCGGCCAGATGAACCAGGTCATCGCCAAGCCGCGCGCCGAGCTGTCCGAGAACGCCCCGACCATGCTGCAGATGAAGATCGACTCGGACAAGATCCGCGACGTCATCGGCAAGGGCGGCGCGACCATCCGTGGTATCTGCGAAGAGACCAAGGCCTCGATCGACATCGAGGACGATGGCAGCGTGAAGATCTACGGCGAAACCAAGGAAGCCGCCGAGGCCGCCAAGCTGCGCGTCCTGGCGATCACCGCGGAAGCCGAGATCGGCAAGATCTACGTCGGCAAGGTGGAGCGCATCGTCGACTTCGGTGCCTTCGTCAACATCCTGCCGGGCAAGGACGGCCTGGTGCACATCTCGCAGATCAGCGACAAGCGCATCGACAAGGTCACCGACGTGCTGCAGGAAGGCCAGGAAGTGAAGGTCCTGGTGCTCGACGTGGACAACCGTGGCCGCATCAAGCTGTCGATCAAGGACGTGGCTGCCGCCGAAGCTTCCGGCGTGTAA
Protein Properties
Protein Residues: 701
Protein Molecular Weight: 75.4 kDa
Protein Theoretical pI: 4.81
Hydropathicity (GRAVY score): -0.07
Charge at pH 7 (predicted): -20.13
Protein Sequence: 
>PA4740
MNPVTKQFQFGQSTVTLETGRIARQATGAVLVTMDDVSVLVTVVGAKSPAEGRDFFPLSVHYQEKTYAAGRIPGGFFKREGRPSEKETLTSRLIDRPIRPLFPEGFMNEVQVVCTVVSTNKKSDPDIAAMIGTSAALAISGIPFAGPIGAARVGFHPEIGYILNPTYEQLQSSSLDMVVAGTEDAVLMVESEADELTEDQMLGAVLFAHDEFQAVIRAVKELAAEAGKPAWDWKAPAENTVLVNAIKAELGEAISQAYTITIKQDRYNRLGELRDQAVALFAGEEEGKFPASEVKDVFGLLEYRTVRENIVNGKPRIDGRDTRTVRPLRIEVGVLGKTHGSALFTRGETQALVVATLGTARDAQLLDTLEGERKDAFMLHYNFPPFSVGECGRMGSPGRREIGHGRLARRGVAAMLPTQDEFPYTIRVVSEITESNGSSSMASVCGASLALMDAGVPVKAPVAGIAMGLVKEGEKFAVLTDILGDEDHLGDMDFKVAGTDKGVTALQMDIKINGITEEIMEIALGQALEARLNILGQMNQVIAKPRAELSENAPTMLQMKIDSDKIRDVIGKGGATIRGICEETKASIDIEDDGSVKIYGETKEAAEAAKLRVLAITAEAEIGKIYVGKVERIVDFGAFVNILPGKDGLVHISQISDKRIDKVTDVLQEGQEVKVLVLDVDNRGRIKLSIKDVAAAEASGV
References
External Links:
Resource Link
Genome ID: PA4740
Entrez Gene ID: 881671
NCBI Protein ID: 15599934
General Reference: PaperBLAST - Find papers about PA4740 and its homologs