4-hydroxy-3-methylbut-2-enyl diphosphate reductase (PA4557)

Identification
Name: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase
Synonyms: Not Available
Gene Name: ispH
Enzyme Class:
Biological Properties
General Function: Involved in isopentenyl diphosphate biosynthetic process, mevalonate-independent pathway
Specific Function: Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). Is also involved in penicillin tolerance and control of the stringent response. Seems to directly or indirectly interact with relA to maintain it in an inactive form during normal growth
Cellular Location: Not Available
KEGG Pathways:
KEGG Reactions:
Thumb+Thumb+Thumb → Thumb+Thumb+Thumb
Hydrogen ion + 1-Hydroxy-2-methyl-2-butenyl 4-diphosphate + NADH → Water + Isopentenyl pyrophosphate + NAD
ReactionCard
Thumb+Thumb+Thumb → Thumb+Thumb+Thumb
Hydrogen ion + 1-Hydroxy-2-methyl-2-butenyl 4-diphosphate + NADH → Dimethylallylpyrophosphate + Water + NAD
ReactionCard
Thumb+Thumb+Thumb ↔ Thumb+Thumb+Thumb
1-Hydroxy-2-methyl-2-butenyl 4-diphosphate + NADPH + Hydrogen ion ↔ Isopentenyl pyrophosphate + NADP + Water
ReactionCard
Thumb+Thumb+Thumb ↔ Thumb+Thumb+Thumb
Dimethylallylpyrophosphate + NADP + Water ↔ 1-Hydroxy-2-methyl-2-butenyl 4-diphosphate + NADPH + Hydrogen ion
ReactionCard
Thumb+Thumb+Thumb ↔ Thumb+Thumb+Thumb
Isopentenyl pyrophosphate + NAD + Water ↔ 1-Hydroxy-2-methyl-2-butenyl 4-diphosphate + NADH + Hydrogen ion
ReactionCard
Thumb+Thumb+Thumb ↔ Thumb+Thumb+Thumb
Dimethylallylpyrophosphate + NAD + Water ↔ 1-Hydroxy-2-methyl-2-butenyl 4-diphosphate + NADH + Hydrogen ion
ReactionCard
Thumb+Thumb+Thumb+Thumb+Thumb ↔ Thumb+Thumb+Thumb+Thumb
Isopentenyl pyrophosphate + NAD + NADP + Water + Dimethylallylpyrophosphate ↔ 1-Hydroxy-2-methyl-2-butenyl 4-diphosphate + NADH + NADPH + Hydrogen ion
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SMPDB Reactions:
Thumb+Thumb+NADPH+Thumb → Thumb+NADPH+Isopentenyl pyrophosphate+Thumb
1-Hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate + Hydrogen ion + NADPH + NADPH → Water + NADPH + Isopentenyl pyrophosphate + Isopentenyl pyrophosphate
ReactionCard
Thumb+Thumb+NADPH+Thumb → Thumb+Thumb+Dimethylallylpyrophosphate+Thumb
1-Hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate + Hydrogen ion + NADPH + NADPH → NADP + Water + Dimethylallylpyrophosphate + Dimethylallylpyrophosphate
ReactionCard
PseudoCyc/BioCyc Reactions:
Thumb+Thumb+Thumb → Thumb+Thumb+Thumb
Hydrogen ion + 1-Hydroxy-2-methyl-2-butenyl 4-diphosphate + NADH → Water + Isopentenyl pyrophosphate + NAD
ReactionCard
Thumb+Thumb+Thumb → Thumb+Thumb+Thumb
Hydrogen ion + 1-Hydroxy-2-methyl-2-butenyl 4-diphosphate + NADH → Dimethylallylpyrophosphate + Water + NAD
ReactionCard
Thumb+Thumb+Thumb ↔ Thumb+Thumb+Thumb
1-Hydroxy-2-methyl-2-butenyl 4-diphosphate + NADPH + Hydrogen ion ↔ Isopentenyl pyrophosphate + NADP + Water
ReactionCard
Thumb+Thumb+Thumb ↔ Thumb+Thumb+Thumb
Dimethylallylpyrophosphate + NADP + Water ↔ 1-Hydroxy-2-methyl-2-butenyl 4-diphosphate + NADPH + Hydrogen ion
ReactionCard
Thumb+Thumb+Thumb ↔ Thumb+Thumb+Thumb
Isopentenyl pyrophosphate + NAD + Water ↔ 1-Hydroxy-2-methyl-2-butenyl 4-diphosphate + NADH + Hydrogen ion
ReactionCard
Thumb+Thumb+Thumb ↔ Thumb+Thumb+Thumb
Dimethylallylpyrophosphate + NAD + Water ↔ 1-Hydroxy-2-methyl-2-butenyl 4-diphosphate + NADH + Hydrogen ion
ReactionCard
Thumb+NAD(P)(+)+Thumb → Thumb+NAD(P)H
Isopentenyl pyrophosphate + NAD(P)(+) + Water → 1-Hydroxy-2-methyl-2-butenyl 4-diphosphate + NAD(P)H
ReactionCard
Thumb+NAD(P)(+)+Thumb → Thumb+NAD(P)H
Dimethylallylpyrophosphate + NAD(P)(+) + Water → 1-Hydroxy-2-methyl-2-butenyl 4-diphosphate + NAD(P)H
ReactionCard
Thumb+Thumb+Thumb+Thumb+Thumb ↔ Thumb+Thumb+Thumb+Thumb
Isopentenyl pyrophosphate + NAD + NADP + Water + Dimethylallylpyrophosphate ↔ 1-Hydroxy-2-methyl-2-butenyl 4-diphosphate + NADH + NADPH + Hydrogen ion
ReactionCard
Thumb+Thumb+NADPH+Thumb → Thumb+NADPH+Isopentenyl pyrophosphate+Thumb
1-Hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate + Hydrogen ion + NADPH + NADPH → Water + NADPH + Isopentenyl pyrophosphate + Isopentenyl pyrophosphate
ReactionCard
Thumb+Thumb+NADPH+Thumb → Thumb+Thumb+Dimethylallylpyrophosphate+Thumb
1-Hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate + Hydrogen ion + NADPH + NADPH → NADP + Water + Dimethylallylpyrophosphate + Dimethylallylpyrophosphate
ReactionCard
Complex Reactions:
Thumb+NAD(P)(+)+Thumb → Thumb+NAD(P)H
Isopentenyl pyrophosphate + NAD(P)(+) + Water → 1-Hydroxy-2-methyl-2-butenyl 4-diphosphate + NAD(P)H
ReactionCard
Thumb+NAD(P)(+)+Thumb → Thumb+NAD(P)H
Dimethylallylpyrophosphate + NAD(P)(+) + Water → 1-Hydroxy-2-methyl-2-butenyl 4-diphosphate + NAD(P)H
ReactionCard
Transports: Not Available
Metabolites:
PAMDB IDNameView
PAMDB0018681-Hydroxy-2-methyl-2-(E)-butenyl 4-diphosphateMetaboCard
PAMDB0008691-Hydroxy-2-methyl-2-butenyl 4-diphosphateMetaboCard
PAMDB000260DimethylallylpyrophosphateMetaboCard
PAMDB001633Hydrogen ionMetaboCard
PAMDB000348Isopentenyl pyrophosphateMetaboCard
PAMDB000197NADMetaboCard
PAMDB000398NADHMetaboCard
PAMDB000089NADPMetaboCard
PAMDB000615NADPHMetaboCard
PAMDB000142WaterMetaboCard
GO Classification:
Process
carboxylic acid metabolic process
cellular metabolic process
isopentenyl diphosphate biosynthetic process, mevalonate-independent pathway
metabolic process
monocarboxylic acid metabolic process
organic acid metabolic process
oxidation reduction
oxoacid metabolic process
pyruvate metabolic process
Gene Properties
Locus tag: PA4557
Strand: -
Entrez Gene ID: 879669
Accession: NP_253247.1
GI: 15599753
Sequence start: 5104986
Sequence End: 5105930
Sequence Length: 944
Gene Sequence: 
>PA4557
ATGCAAATCAAACTCGCCAATCCCCGCGGCTTCTGCGCCGGCGTGGATCGCGCCATCGAGATCGTCAACCGTGCCCTCGATGTCTTCGGTCCGCCGATCTACGTGCGTCACGAGGTGGTGCACAACAAGTTCGTCGTGGACAACCTGCGCCAGCGCGGCGCCATCTTCGTCGAGGAACTCGATCAGGTGCCGAACAACGTCATCGTCATCTTCAGCGCCCACGGCGTTTCCCAGGCGGTCCGCAAGGAAGCCGAGGGGCGCGGCCTGAAGGTTTTCGACGCGACCTGCCCGCTGGTGACCAAAGTGCACATGGAAGTGGTGCGCTACAGCCGCGACGGCCACGAATGCGTGCTGATCGGGCATGAAGGGCACCCCGAGGTGGAAGGCACCATGGGCCAGTACGATGCCAGCAACGGTGGTGCCATCTACCTAGTGGAGGACGAGGCCGACGTCGCCGCGCTGGAGGTGCGCAAGCCCGAAGCCCTGCACTACGTGACCCAGACGACCCTGTCGATGGACGACACCTCGAAGGTCATCGATGCCCTGCGCGCCAAGTTCCCGCAGATCCAGGGACCGCGCAAGAACGACATCTGCTATGCCACCCAGAACCGCCAGGATGCCGTGAAGGAACTGGCCGACCAGTGCGACATGGTCCTGGTGGTGGGCAGCCCCAACAGTTCCAACTCCAACCGCCTGCGCGAACTCGCCGAGCGGATGGGCACGCCGGCCTACCTGATCGACGGCGCCGAGGACATGCAACGCGGCTGGTTCGACGGTGTGCGTCGCATCGGAATCACCGCAGGCGCTTCCGCGCCGGAAGTGCTGGTGCGCGGCGTGATCGCCCAGCTACGCGAGTGGGGGGCGTCGGAGGAGCAGGAACTGGAGGGGCGGGAGGAGAACATTACCTTCTCGATGCCCAAGGAACTGCGGGTCAAGGCTTTGTGA
Protein Properties
Protein Residues: 314
Protein Molecular Weight: 34.8 kDa
Protein Theoretical pI: 5.15
Hydropathicity (GRAVY score): -0.277
Charge at pH 7 (predicted): -9.21
Protein Sequence: 
>PA4557
MQIKLANPRGFCAGVDRAIEIVNRALDVFGPPIYVRHEVVHNKFVVDNLRQRGAIFVEELDQVPNNVIVIFSAHGVSQAVRKEAEGRGLKVFDATCPLVTKVHMEVVRYSRDGHECVLIGHEGHPEVEGTMGQYDASNGGAIYLVEDEADVAALEVRKPEALHYVTQTTLSMDDTSKVIDALRAKFPQIQGPRKNDICYATQNRQDAVKELADQCDMVLVVGSPNSSNSNRLRELAERMGTPAYLIDGAEDMQRGWFDGVRRIGITAGASAPEVLVRGVIAQLREWGASEEQELEGREENITFSMPKELRVKAL
References
External Links:
Resource Link
Genome ID: PA4557
Entrez Gene ID: 879669
NCBI Protein ID: 15599753
General Reference: PaperBLAST - Find papers about PA4557 and its homologs