Identification
Name: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase
Synonyms: Not Available
Gene Name: ispH
Enzyme Class:
Biological Properties
General Function: Involved in isopentenyl diphosphate biosynthetic process, mevalonate-independent pathway
Specific Function: Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). Is also involved in penicillin tolerance and control of the stringent response. Seems to directly or indirectly interact with relA to maintain it in an inactive form during normal growth
Cellular Location: Not Available
KEGG Pathways:
KEGG Reactions:
Thumb+Thumb+ThumbThumb+Thumb+Thumb
Thumb+Thumb+ThumbThumb+Thumb+Thumb
Thumb+Thumb+ThumbThumb+Thumb+Thumb
Thumb+Thumb+ThumbThumb+Thumb+Thumb
Thumb+Thumb+ThumbThumb+Thumb+Thumb
Thumb+Thumb+ThumbThumb+Thumb+Thumb
Thumb+Thumb+Thumb+Thumb+ThumbThumb+Thumb+Thumb+Thumb
SMPDB Reactions:
Thumb+Thumb+NADPH+ThumbThumb+NADPH+Isopentenyl pyrophosphate+Thumb
Thumb+Thumb+NADPH+ThumbThumb+Thumb+Dimethylallylpyrophosphate+Thumb
PseudoCyc/BioCyc Reactions:
Thumb+Thumb+ThumbThumb+Thumb+Thumb
Thumb+Thumb+ThumbThumb+Thumb+Thumb
Thumb+Thumb+ThumbThumb+Thumb+Thumb
Thumb+Thumb+ThumbThumb+Thumb+Thumb
Thumb+Thumb+ThumbThumb+Thumb+Thumb
Thumb+Thumb+ThumbThumb+Thumb+Thumb
Thumb+NAD(P)(+)+ThumbThumb+NAD(P)H
Thumb+NAD(P)(+)+ThumbThumb+NAD(P)H
Thumb+Thumb+Thumb+Thumb+ThumbThumb+Thumb+Thumb+Thumb
Thumb+Thumb+NADPH+ThumbThumb+NADPH+Isopentenyl pyrophosphate+Thumb
Thumb+Thumb+NADPH+ThumbThumb+Thumb+Dimethylallylpyrophosphate+Thumb
Complex Reactions:
Thumb+NAD(P)(+)+ThumbThumb+NAD(P)H
Thumb+NAD(P)(+)+ThumbThumb+NAD(P)H
Transports: Not Available
Metabolites:
PAMDB IDNameView
PAMDB0018681-Hydroxy-2-methyl-2-(E)-butenyl 4-diphosphateMetaboCard
PAMDB0008691-Hydroxy-2-methyl-2-butenyl 4-diphosphateMetaboCard
PAMDB000260DimethylallylpyrophosphateMetaboCard
PAMDB001633Hydrogen ionMetaboCard
PAMDB000348Isopentenyl pyrophosphateMetaboCard
PAMDB000197NADMetaboCard
PAMDB000398NADHMetaboCard
PAMDB000089NADPMetaboCard
PAMDB000615NADPHMetaboCard
PAMDB000142WaterMetaboCard
GO Classification:
Process
carboxylic acid metabolic process
cellular metabolic process
isopentenyl diphosphate biosynthetic process, mevalonate-independent pathway
metabolic process
monocarboxylic acid metabolic process
organic acid metabolic process
oxidation reduction
oxoacid metabolic process
pyruvate metabolic process
Gene Properties
Locus tag: PA4557
Strand: -
Entrez Gene ID: 879669
Accession: NP_253247.1
GI: 15599753
Sequence start: 5104986
Sequence End: 5105930
Sequence Length: 944
Gene Sequence:
>PA4557
ATGCAAATCAAACTCGCCAATCCCCGCGGCTTCTGCGCCGGCGTGGATCGCGCCATCGAGATCGTCAACCGTGCCCTCGATGTCTTCGGTCCGCCGATCTACGTGCGTCACGAGGTGGTGCACAACAAGTTCGTCGTGGACAACCTGCGCCAGCGCGGCGCCATCTTCGTCGAGGAACTCGATCAGGTGCCGAACAACGTCATCGTCATCTTCAGCGCCCACGGCGTTTCCCAGGCGGTCCGCAAGGAAGCCGAGGGGCGCGGCCTGAAGGTTTTCGACGCGACCTGCCCGCTGGTGACCAAAGTGCACATGGAAGTGGTGCGCTACAGCCGCGACGGCCACGAATGCGTGCTGATCGGGCATGAAGGGCACCCCGAGGTGGAAGGCACCATGGGCCAGTACGATGCCAGCAACGGTGGTGCCATCTACCTAGTGGAGGACGAGGCCGACGTCGCCGCGCTGGAGGTGCGCAAGCCCGAAGCCCTGCACTACGTGACCCAGACGACCCTGTCGATGGACGACACCTCGAAGGTCATCGATGCCCTGCGCGCCAAGTTCCCGCAGATCCAGGGACCGCGCAAGAACGACATCTGCTATGCCACCCAGAACCGCCAGGATGCCGTGAAGGAACTGGCCGACCAGTGCGACATGGTCCTGGTGGTGGGCAGCCCCAACAGTTCCAACTCCAACCGCCTGCGCGAACTCGCCGAGCGGATGGGCACGCCGGCCTACCTGATCGACGGCGCCGAGGACATGCAACGCGGCTGGTTCGACGGTGTGCGTCGCATCGGAATCACCGCAGGCGCTTCCGCGCCGGAAGTGCTGGTGCGCGGCGTGATCGCCCAGCTACGCGAGTGGGGGGCGTCGGAGGAGCAGGAACTGGAGGGGCGGGAGGAGAACATTACCTTCTCGATGCCCAAGGAACTGCGGGTCAAGGCTTTGTGA
Protein Properties
Protein Residues: 314
Protein Molecular Weight: 34.8 kDa
Protein Theoretical pI: 5.15
Hydropathicity (GRAVY score): -0.277
Charge at pH 7 (predicted): -9.21
Protein Sequence:
>PA4557
MQIKLANPRGFCAGVDRAIEIVNRALDVFGPPIYVRHEVVHNKFVVDNLRQRGAIFVEELDQVPNNVIVIFSAHGVSQAVRKEAEGRGLKVFDATCPLVTKVHMEVVRYSRDGHECVLIGHEGHPEVEGTMGQYDASNGGAIYLVEDEADVAALEVRKPEALHYVTQTTLSMDDTSKVIDALRAKFPQIQGPRKNDICYATQNRQDAVKELADQCDMVLVVGSPNSSNSNRLRELAERMGTPAYLIDGAEDMQRGWFDGVRRIGITAGASAPEVLVRGVIAQLREWGASEEQELEGREENITFSMPKELRVKAL
References
External Links:
Resource Link
Genome ID: PA4557
Entrez Gene ID: 879669
NCBI Protein ID: 15599753
General Reference: PaperBLAST - Find papers about PA4557 and its homologs