2,5-diketo-D-gluconic acid reductase B (PA4167)

Identification
Name: 2,5-diketo-D-gluconic acid reductase B
Synonyms:
  • 2,5-DKG reductase B
  • 2,5-DKGR B
  • 25DKGR-B
  • AKR5D
Gene Name: dkgB
Enzyme Class:
Biological Properties
General Function: Involved in oxidoreductase activity
Specific Function: Catalyzes the reduction of 2,5-diketo-D-gluconic acid (25DKG) to 2-keto-L-gulonic acid (2KLG)
Cellular Location: Cytoplasm
KEGG Pathways: Not Available
KEGG Reactions:
Thumb ↔ Thumb+Thumb+Thumb
NADP ↔ 2,5-Diketo-D-gluconate + NADPH + Hydrogen ion
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Thumb+Thumb ↔ Thumb+Thumb+Thumb
2-Dehydro-D-gluconate + NADP ↔ 2,5-Diketo-D-gluconate + NADPH + Hydrogen ion
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SMPDB Reactions:
Thumb+Thumb+NADPH+Thumb → Thumb+2-Keto-L-gluconate+Thumb
2,5-Diketo-D-gluconate + Hydrogen ion + NADPH + NADPH → NADP + 2-Keto-L-gluconate + 2-Dehydro-D-gluconate
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Thumb+Thumb+Thumb ↔ Hydroxyacetone+Thumb
Pyruvaldehyde + NADPH + Hydrogen ion ↔ Hydroxyacetone + NADP
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Thumb+Thumb+Thumb → Thumb+Thumb
Pyruvaldehyde + NADPH + Hydrogen ion → Lactaldehyde + NADP
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PseudoCyc/BioCyc Reactions:
Thumb+Thumb+Thumb ↔ Thumb+Thumb
Hydrogen ion + NADPH + 2,5-Diketo-D-gluconate ↔ 2-Dehydro-D-gluconate + NADP
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Thumb+Thumb+Thumb → acetol+Thumb
Hydrogen ion + Pyruvaldehyde + NADPH → acetol + NADP
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Complex Reactions:
Thumb+Thumb+Thumb → 2 -Dehydro-L-gulonate+Thumb
2,5-Diketo-D-gluconate + Hydrogen ion + NADPH → 2 -Dehydro-L-gulonate + NADP
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Thumb+Thumb+Thumb → Acetol+Thumb
Hydrogen ion + Pyruvaldehyde + NADPH → Acetol + NADP
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Transports: Not Available
Metabolites:
PAMDB IDNameView
PAMDB0005922,5-Diketo-D-gluconateMetaboCard
PAMDB0005752-Dehydro-D-gluconateMetaboCard
PAMDB001633Hydrogen ionMetaboCard
PAMDB000476LactaldehydeMetaboCard
PAMDB000089NADPMetaboCard
PAMDB000615NADPHMetaboCard
PAMDB000281PyruvaldehydeMetaboCard
GO Classification:
Function
catalytic activity
oxidoreductase activity
Process
metabolic process
oxidation reduction
Gene Properties
Locus tag: PA4167
Strand: -
Entrez Gene ID: 880245
Accession: NP_252856.1
GI: 15599362
Sequence start: 4662693
Sequence End: 4663511
Sequence Length: 818
Gene Sequence: 
>PA4167
ATGTCGTCCACCATCCACCCCCTCCCGTCCTTCGGTCTCGGCACCTTCCGCCTTACTGGCCAGGTCGTCGTCGACTCGGTGCGCAGCGCCCTCGAACTGGGCTATCGCGCCATCGATACCGCGCAGATCTACGGCAACGAGGCGGATATCGGCCGCGCGATCGCCGAGAGCGGCGTACCGCGTTCCGAGCTGTTCCTCACCACCAAGGTCTGGGTCGACAACTACGCCCGCGAAAAGCTGCTCGCCAGCCTGCGCGAGAGTCTGGAGAAACTGCGCACCGACCGGTTGGACCTGGTCCTGATCCATTGGCCGGCACCCGGCAACGGCGTCGAGCTGGGGGAGTACATGGCGGCGCTGGCGGAAGCCAAGTCCCTGGGCCTGACCCGGCGGATCGGCGTCTCCAACTTCAACATCGAACTGACCCGCCAGGCCATCGCCGCGGTCGGTGCGGGCGAGATATCCACCAACCAGATCGAACTCAGCCCTTACTTGCAGAACCGCGCGCTGACCGCCTACCTCGAGGAGCAGGGCATCGCGGTCACCTCGTACATGACCCTGGCCTACGGCAAGGTGCTGAAGGACCCGACGCTGGCCGGGATAGCCGCCAGGCACCGGGCCACGGTCGCCCAGGTGGCGCTGGCCTGGGCGATGCAACTGGGCTACGCGGTGATTCCGTCCTCGACCCGCCGCGAGAACCTCGCCAGCAACCTGCTGGCGCGCGACCTGCGCCTGGACGCCGAGGACATGGCGCGCATCGCCGGCCTCGAGCGTAACGGCCGCGAGGTCAGTCCGCAGGGCCTGGCGCCGGCCTGGGACTGA
Protein Properties
Protein Residues: 272
Protein Molecular Weight: 29.5 kDa
Protein Theoretical pI: 6.3
Hydropathicity (GRAVY score): -0.021
Charge at pH 7 (predicted): -1.28
Protein Sequence: 
>PA4167
MSSTIHPLPSFGLGTFRLTGQVVVDSVRSALELGYRAIDTAQIYGNEADIGRAIAESGVPRSELFLTTKVWVDNYAREKLLASLRESLEKLRTDRLDLVLIHWPAPGNGVELGEYMAALAEAKSLGLTRRIGVSNFNIELTRQAIAAVGAGEISTNQIELSPYLQNRALTAYLEEQGIAVTSYMTLAYGKVLKDPTLAGIAARHRATVAQVALAWAMQLGYAVIPSSTRRENLASNLLARDLRLDAEDMARIAGLERNGREVSPQGLAPAWD
References
External Links:
Resource Link
Genome ID: PA4167
Entrez Gene ID: 880245
NCBI Protein ID: 15599362
General Reference: PaperBLAST - Find papers about PA4167 and its homologs