NAD-dependent malic enzyme (PA3471)

Identification
Name: NAD-dependent malic enzyme
Synonyms:
  • NAD-ME
Gene Name: sfcA
Enzyme Class:
Biological Properties
General Function: Involved in oxidoreductase activity
Specific Function: (S)-malate + NAD(+) = pyruvate + CO(2) + NADH
Cellular Location: Not Available
KEGG Pathways:
KEGG Reactions:
Thumb+Thumb → Thumb+Thumb+Thumb
L-Malic acid + NAD → Carbon dioxide + NADH + Pyruvic acid
ReactionCard
Thumb+Thumb ↔ Thumb+Thumb+Thumb+Thumb
L-Malic acid + NAD ↔ Pyruvic acid + Carbon dioxide + NADH + Hydrogen ion
ReactionCard
Thumb+Thumb+Thumb ↔ Thumb+Thumb+Thumb
L-Malic acid + NAD + Oxalacetic acid ↔ Pyruvic acid + Carbon dioxide + NADH
ReactionCard
SMPDB Reactions:
L-Malic acid+Thumb+Thumb → Thumb+Thumb+Thumb
L-Malic acid + NAD + L-Malic acid → Carbon dioxide + NADH + Pyruvic acid
ReactionCard
PseudoCyc/BioCyc Reactions:
Thumb+Thumb → Thumb+Thumb+Thumb
L-Malic acid + NAD → Carbon dioxide + NADH + Pyruvic acid
ReactionCard
Complex Reactions:
Thumb+Thumb → Thumb+Thumb+Thumb
L-Malic acid + NAD → Pyruvic acid + Carbon dioxide + NADH
ReactionCard
Transports: Not Available
Metabolites:
PAMDB IDNameView
PAMDB000568Carbon dioxideMetaboCard
PAMDB001633Hydrogen ionMetaboCard
PAMDB000058L-Malic acidMetaboCard
PAMDB000197NADMetaboCard
PAMDB000398NADHMetaboCard
PAMDB000102Pyruvic acidMetaboCard
GO Classification:
Function
binding
catalytic activity
cation binding
ion binding
malate dehydrogenase activity
malic enzyme activity
metal ion binding
NAD or NADH binding
nucleotide binding
oxidoreductase activity
oxidoreductase activity, acting on CH-OH group of donors
oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
Process
carboxylic acid metabolic process
cellular metabolic process
dicarboxylic acid metabolic process
malate metabolic process
metabolic process
organic acid metabolic process
oxidation reduction
oxoacid metabolic process
Gene Properties
Locus tag: PA3471
Strand: -
Entrez Gene ID: 879925
Accession: NP_252161.1
GI: 15598667
Sequence start: 3883560
Sequence End: 3885254
Sequence Length: 1694
Gene Sequence: 
>PA3471
ATGACCGAAACAGCCAAGCGTCCCTTGTACGTTCCCCATGCCGGCCCATCGCTGCTGGAGATGCCGCTGCTGAACAAAGGCAGCGCGTTCAGTACCCAGGAACGCATCGATTTCAACCTGCAGGGCCTGCTGCCGCACAACATCGAGACCATCGAGGAGCAGACCGAGCGCGCCTACAGCCAGTACAACCTGTGCAACACCGATCTGGACCGCCACATCTTCCTGCGCTCGATCCAGGACAACAACGAGACCCTGTTCTTCCGCCTGCTCGAGGAGCACCTGGAAGAAATGATGCCGATCATCTACACCCCCACGGTCGGCCAGGCCTGCCAGGAGTTCTCGAAGATCTACCGGACCCACCGCGGCCTGTTCATCTCCTACCCGGACCGCGAGCGGATCGACGACATCCTGCGCAGCGCCACCAAGAACAACGTGAAGATCGTGGTGGTCACCGACAGCGAGCGGATCCTCGGCCTCGGCGACCAGGGCATCGGCGGGATGGGCATCCCGATCGGCAAGCTGTCCCTGTACACCGCCTGCGGCGGTATCAGCCCGGCCTACACCCTGCCGGTGGTGCTGGACGTCGGCACCAACAACCCGGACCTGCTCAACGACCCGATGTACATGGGCTGGCGCCACGAGCGGGTGAGCGGGGCGCAGTACGAGGAGTTCGTCGACCTGTTCATCCAGGCGATCAAGCGCCGCTGGCCCAACGTCCTGCTGCAATTCGAGGACTTCGCCCAGACCAATGCCATGCCGTTGCTGGAGCGCTACAAGGACGAGCTGTGCTGCTTCAACGACGACATCCAGGGCACCGCCGCGGTGGCCGTGGGCACCCTGCTGGCGGCTTGCAAGGCCAAGGGCGAGAAGCTCAGCGAGCAGACCGTGACCTTCGTCGGCGCCGGCTCCGCCGGTTGCGGCATCGCCGAACAGATCATCGCCGCCATGCAACTGGAGGGCCTGGACGAGGCCCAGGCGCGTCGGCGCATCTTCATGGTCGACCGCTGGGGCCTGCTCACCGACGACATGAGCAACCTGCTCGACTTCCAGCACCGCCTGGCGCAGAAGCGCGCCGATCTCGGTGCCTGGGGCGGCCAGCAGGGCGACGACCTGGCGTTGCTGGAAGTGATCCGCAATGCCCGGCCGACGGTGCTGATCGGCGTCTCCGGGCAGCGCGGGCTGTTTTCCGAAGAGGTCATCCGTGAGCTGCACAGCCATTGCAAGCAGCCGCTGGTGATGCCGCTGTCCAACCCGACCTCGCGGGTCGAGGCGACTCCGCAGGAAATCCTCAACTGGACCGACGGCCAGGCGCTGGTCGCCACCGGTAGCCCGTTCCAACCGGTGCAGGTGGGCGACAAGCGGATTCCCATCGCCCAGTGCAACAACGCCTATATCTTCCCCGGCATCGGCCTGGGGGTGATCGCCGCGCGGGCCAATCGGGTCACCGAAGGCATGCTGATGGCCGCGGCCAACGCCCTGGCCAACTGCTCGCCGATCGTTACCCAGGGCGAGGGCGCCGTGTTGCCGGCGCTGGGCGACATCCGCGAGGTCAGCAAGCGCATCGCCGTCGCCGTGGCCAAGCAGGCCCAGGCCGAGGGCAAGGCCCTGCATACCAGCGACGAGGTACTGAACGACGCCATCGAGGCGAACTTCTGGTTCCCGCGCTACCGTGCCTACCGCCGGACTTCGTTCTGA
Protein Properties
Protein Residues: 564
Protein Molecular Weight: 62.4 kDa
Protein Theoretical pI: 5.18
Hydropathicity (GRAVY score): -0.154
Charge at pH 7 (predicted): -11.87
Protein Sequence: 
>PA3471
MTETAKRPLYVPHAGPSLLEMPLLNKGSAFSTQERIDFNLQGLLPHNIETIEEQTERAYSQYNLCNTDLDRHIFLRSIQDNNETLFFRLLEEHLEEMMPIIYTPTVGQACQEFSKIYRTHRGLFISYPDRERIDDILRSATKNNVKIVVVTDSERILGLGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNPDLLNDPMYMGWRHERVSGAQYEEFVDLFIQAIKRRWPNVLLQFEDFAQTNAMPLLERYKDELCCFNDDIQGTAAVAVGTLLAACKAKGEKLSEQTVTFVGAGSAGCGIAEQIIAAMQLEGLDEAQARRRIFMVDRWGLLTDDMSNLLDFQHRLAQKRADLGAWGGQQGDDLALLEVIRNARPTVLIGVSGQRGLFSEEVIRELHSHCKQPLVMPLSNPTSRVEATPQEILNWTDGQALVATGSPFQPVQVGDKRIPIAQCNNAYIFPGIGLGVIAARANRVTEGMLMAAANALANCSPIVTQGEGAVLPALGDIREVSKRIAVAVAKQAQAEGKALHTSDEVLNDAIEANFWFPRYRAYRRTSF
References
External Links:
Resource Link
Genome ID: PA3471
Entrez Gene ID: 879925
NCBI Protein ID: 15598667
General Reference: PaperBLAST - Find papers about PA3471 and its homologs